CA2817011C - Variant, recombinant beta-glucocerebrosidase proteins with increased stability and increased retained catalytic activity - Google Patents

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Publication number

CA2817011C

CA2817011C CA2817011A CA2817011A CA2817011C CA 2817011 C CA2817011 C CA 2817011C CA 2817011 A CA2817011 A CA 2817011A CA 2817011 A CA2817011 A CA 2817011A CA 2817011 C CA2817011 C CA 2817011C

Authority

CA

Canada

Prior art keywords

leu

ala

ser

gly

pro

Prior art date

2010-11-08

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

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• 230000000717 retained effect Effects 0.000 title claims description 90
• 108010017544 Glucosylceramidase Proteins 0.000 title abstract description 18
• 102000004547 Glucosylceramidase Human genes 0.000 title abstract description 18
• 208000015439 Lysosomal storage disease Diseases 0.000 claims abstract description 31
• 108090000623 proteins and genes Proteins 0.000 claims description 271
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• 241000282414 Homo sapiens Species 0.000 claims description 37
• 230000007935 neutral effect Effects 0.000 claims description 33
• 125000003275 alpha amino acid group Chemical group 0.000 claims description 29
• 238000006467 substitution reaction Methods 0.000 claims description 29
• 238000011282 treatment Methods 0.000 claims description 14
• 208000015872 Gaucher disease Diseases 0.000 claims description 12
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• 239000000203 mixture Substances 0.000 claims description 8
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