CA2766351C - Chimeric primers with hairpin conformations and methods of using same - Google Patents

Chimeric primers with hairpin conformations and methods of using same Download PDF

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CA2766351C
CA2766351C CA2766351A CA2766351A CA2766351C CA 2766351 C CA2766351 C CA 2766351C CA 2766351 A CA2766351 A CA 2766351A CA 2766351 A CA2766351 A CA 2766351A CA 2766351 C CA2766351 C CA 2766351C
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sequence
tag
nucleic acid
tag sequence
primer
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CA2766351A1 (en
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Douglas F. Whitman
Hongwei Zhang
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Luminex Corp
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6818Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6823Release of bound markers
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2766351A 2009-06-29 2010-06-29 Chimeric primers with hairpin conformations and methods of using same Active CA2766351C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US22127109P 2009-06-29 2009-06-29
US61/221,271 2009-06-29
PCT/US2010/040354 WO2011008530A2 (en) 2009-06-29 2010-06-29 Chimeric primers with hairpin conformations and methods of using same

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CA2766351A1 CA2766351A1 (en) 2011-01-20
CA2766351C true CA2766351C (en) 2018-02-27

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US (5) US9587270B2 (OSRAM)
EP (2) EP2449104B1 (OSRAM)
JP (3) JP5785942B2 (OSRAM)
CN (1) CN102686728B (OSRAM)
CA (1) CA2766351C (OSRAM)
WO (1) WO2011008530A2 (OSRAM)

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