CA2760768C - Modele de tissu pulmonaire - Google Patents

Modele de tissu pulmonaire Download PDF

Info

Publication number
CA2760768C
CA2760768C CA2760768A CA2760768A CA2760768C CA 2760768 C CA2760768 C CA 2760768C CA 2760768 A CA2760768 A CA 2760768A CA 2760768 A CA2760768 A CA 2760768A CA 2760768 C CA2760768 C CA 2760768C
Authority
CA
Canada
Prior art keywords
cells
pulmonary
tissue culture
model
dimensional
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2760768A
Other languages
English (en)
Other versions
CA2760768A1 (fr
Inventor
Judit Erzsebet Pongracz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
PECS, University of
Original Assignee
PECS, University of
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by PECS, University of filed Critical PECS, University of
Publication of CA2760768A1 publication Critical patent/CA2760768A1/fr
Application granted granted Critical
Publication of CA2760768C publication Critical patent/CA2760768C/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5082—Supracellular entities, e.g. tissue, organisms
    • G01N33/5088—Supracellular entities, e.g. tissue, organisms of vertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0688—Cells from the lungs or the respiratory tract
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1323—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/28—Vascular endothelial cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C12N2503/04—Screening or testing on artificial tissues

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Organic Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Pulmonology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L'invention concerne une culture tissulaire d'un modèle pulmonaire tridimensionnel dépourvu de structure tissulaire mise au point par génie tissulaire, qui est exempte de toute structure artificielle; des modèles tridimensionnels de tissu pulmonaire sain ainsi que des tissus pathologiques. Le produit de l'invention peut être commercialisé, p. ex. sous la forme de cultures tissulaires, de plaques ou de réseaux comprenant ces cultures ou de trousses. L'invention peut être appliquée dans la recherche médicale et scientifique pour tester l'effet de composés sur un tissu pulmonaire, à des fins de criblage, d'essai et/ou d'évaluation de médicaments, et dans certains cas pour diagnostiquer des maladies pulmonaires.
CA2760768A 2009-05-05 2010-05-05 Modele de tissu pulmonaire Active CA2760768C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
HUP0900819 2009-05-05
HU0900819A HUP0900819A2 (en) 2009-05-05 2009-05-05 Lung tissue culture
PCT/IB2010/051978 WO2010128464A1 (fr) 2009-05-05 2010-05-05 Modèle de tissu pulmonaire

Publications (2)

Publication Number Publication Date
CA2760768A1 CA2760768A1 (fr) 2010-11-11
CA2760768C true CA2760768C (fr) 2019-10-29

Family

ID=89989476

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2760768A Active CA2760768C (fr) 2009-05-05 2010-05-05 Modele de tissu pulmonaire

Country Status (7)

Country Link
JP (1) JP2013504303A (fr)
CN (1) CN102369277B (fr)
AU (1) AU2010244121B2 (fr)
CA (1) CA2760768C (fr)
HU (1) HUP0900819A2 (fr)
SG (1) SG175425A1 (fr)
WO (1) WO2010128464A1 (fr)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HUE029316T2 (en) 2010-11-04 2017-03-28 Univ Of Pecs Tüdõszövetmodell
JP5778412B2 (ja) * 2010-11-29 2015-09-16 花王株式会社 Ｃｇｒｐ応答調節剤の評価又は選択方法
TWI438145B (zh) * 2011-12-08 2014-05-21 中原大學 連續式產氫裝置及其方法
US20140099709A1 (en) * 2012-06-19 2014-04-10 Organovo, Inc. Engineered three-dimensional connective tissue constructs and methods of making the same
JP6463275B2 (ja) * 2013-01-08 2019-01-30 イエール ユニバーシティ ヒトおよび大型哺乳動物の肺のバイオリアクター
WO2015061372A1 (fr) 2013-10-21 2015-04-30 Hemoshear, Llc Modèle in vitro pour micro-environnement tumoral
CN104316661B (zh) * 2014-10-08 2016-03-23 清华大学 用于生物毒性检测的肺组织模型及生物毒性检测方法
CA2972226A1 (fr) * 2014-12-24 2016-06-30 Ube Industries, Ltd. Fluide de surnageant de culture de cellules derive de tissu pulmonaire
US20190128870A1 (en) * 2016-04-04 2019-05-02 Humeltis Diagnostic Methods For Patient Specific Therapeutic Decision Making In Cancer Care
CN106754669B (zh) * 2016-11-23 2020-04-17 河海大学常州校区 基于反应-扩散模型的多细胞结构的制备方法及制备系统
CN107267439A (zh) * 2017-05-16 2017-10-20 苏州大学 三维肺微组织模型的构建方法及应用
CN108660076A (zh) * 2018-05-21 2018-10-16 中国科学院苏州生物医学工程技术研究所 一种仿真肺芯片模型
CN109161531A (zh) * 2018-10-16 2019-01-08 首都医科大学附属北京胸科医院 一种基于类器官技术个体化肺癌原代细胞培养的方法
GB202005154D0 (en) * 2020-04-07 2020-05-20 Univ Of Hertfordshire Higher Education Corporation Method and model

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6828111B2 (en) * 2000-01-13 2004-12-07 Wayne State University Three-dimensional in vitro model of human preneoplastic breast disease
WO2003010302A1 (fr) * 2001-07-26 2003-02-06 Kazunori Kataoka Construction cellulaire cultivee contenant des spheroides de cellules animales cultivees et utilisation correspondante
WO2007083504A1 (fr) * 2003-08-01 2007-07-26 Norimasa Nakamura Tissu synthétique en 3d auto-organisé sans échafaudage
US7709256B2 (en) * 2004-04-28 2010-05-04 Vaxdesign Corp. Disease model incorporation into an artificial immune system (AIS)
US8628964B2 (en) * 2006-10-11 2014-01-14 Drexel University Fetal pulmonary cells and uses thereof
US20100034791A1 (en) * 2007-02-14 2010-02-11 Drexel University Engineered Lung Tissue Construction for High Throughput Toxicity Screening and Drug Discovery

Also Published As

Publication number Publication date
WO2010128464A1 (fr) 2010-11-11
CN102369277B (zh) 2014-12-17
SG175425A1 (en) 2011-12-29
CN102369277A (zh) 2012-03-07
AU2010244121B2 (en) 2015-07-16
HUP0900819A2 (en) 2011-01-28
JP2013504303A (ja) 2013-02-07
CA2760768A1 (fr) 2010-11-11
AU2010244121A1 (en) 2011-12-15
HU0900819D0 (en) 2010-07-28

Similar Documents

Publication Publication Date Title
CA2760768C (fr) Modele de tissu pulmonaire
US9151744B2 (en) Lung tissue model
JP2021508249A (ja) 免疫細胞オルガノイド共培養物
Choi et al. Organogenesis of adult lung in a dish: Differentiation, disease and therapy
EP3699275A1 (fr) Organoïde d'organe et procédé de production associé
JP2018506971A (ja) 胃腸上皮組織構築物を生成する方法 ナンシー・オールブリトン（Ｎａｎｃｙ Ａｌｌｂｒｉｔｔｏｎ）、ユリ・ワン（Ｙｕｌｉ Ｗａｎｇ）、クリストファー・シムズ（Ｃｈｒｉｓｔｏｐｈｅｒ Ｓｉｍｓ）、スコット・マグネス（Ｓｃｏｔｔ Ｍａｇｎｅｓｓ）及びスコット・ブルトマン（Ｓｃｏｔｔ Ｂｕｌｔｍａｎ）
JP2017524377A (ja) 単一細胞由来オルガノイド
ES2664375T3 (es) Precursores de células estrelladas hepáticas y métodos para aislarlos
TW202039828A (zh) 肝細胞擴增方法
Cao et al. Understanding the role of the microenvironment during definitive hemopoietic development
TW202200786A (zh) 自肺上皮細胞或肺癌細胞製造類器官之方法
CN111876371A (zh) 心脏细胞培养材料
Clause et al. A Three-Dimensional Gel Bioreactor for Assessment of Cardiomyocyte Induction in Skeletal Muscle–Derived Stem Cells
JP7274683B2 (ja) 多能性幹細胞から樹状分岐した集合管を伴う腎臓構造を作製する方法
US9687512B2 (en) Isolated cardiac stem cells and methods of their use
EP3927814A1 (fr) Procédés pour la maturation de cardiomyocytes sur des ecm dérivées de cellules de luquide amniotique, constructions cellulaires et utilisations pour la cardiotoxicité et le criblage proarythmique de composés médicamenteux
JP2008206510A (ja) 腸管幹／前駆細胞の取得方法
Perugini et al. A Substrate-Mimicking Basement Membrane Drives the Organization of Human Mesenchymal Stromal Cells and Endothelial Cells Into Perivascular Niche-Like Structures
JP7576647B2 (ja) 肝臓オルガノイド疾患モデルおよびその作製方法
Ochiai et al. Posology and Serum-/Xeno-Free Engineered Adipose Stromal Cells Cell Sheets
Forghani Fabrication of a Quasi-3D Vascularized Bone Like Construct
TW202417612A (zh) 非真皮乳突細胞至真皮乳突細胞之轉分化(transdifferentiation)
CN114317398A (zh) Gli1和EpCAM基因共同标记的肝祖细胞群及其应用
Costa Adipose stromal vascular fraction cell sheets as vascular strategies for tissue engineering and regenerative medicine applications
Costea Epithelial-mesenchymal interactions in normal and neoplastic human oral mucosa. A study on in vitro organotypic models

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20150501