CA2693102C - Tailored multi-site combinatorial assembly - Google Patents

Tailored multi-site combinatorial assembly Download PDF

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Publication number
CA2693102C
CA2693102C CA2693102A CA2693102A CA2693102C CA 2693102 C CA2693102 C CA 2693102C CA 2693102 A CA2693102 A CA 2693102A CA 2693102 A CA2693102 A CA 2693102A CA 2693102 C CA2693102 C CA 2693102C
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Canada
Prior art keywords
primers
primer
template
group
polynucleotide
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Active
Application number
CA2693102A
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English (en)
French (fr)
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CA2693102A1 (en
Inventor
Xuqiu Tan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF Enzymes LLC
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BASF Enzymes LLC
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=40304880&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2693102(C) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by BASF Enzymes LLC filed Critical BASF Enzymes LLC
Publication of CA2693102A1 publication Critical patent/CA2693102A1/en
Application granted granted Critical
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA2693102A 2007-07-31 2008-07-31 Tailored multi-site combinatorial assembly Active CA2693102C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US95317107P 2007-07-31 2007-07-31
US60/953,171 2007-07-31
PCT/US2008/071771 WO2009018449A1 (en) 2007-07-31 2008-07-31 Tailored multi-site combinatorial assembly

Publications (2)

Publication Number Publication Date
CA2693102A1 CA2693102A1 (en) 2009-02-05
CA2693102C true CA2693102C (en) 2020-03-10

Family

ID=40304880

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2693102A Active CA2693102C (en) 2007-07-31 2008-07-31 Tailored multi-site combinatorial assembly

Country Status (16)

Country Link
US (1) US9476078B2 (https=)
EP (2) EP3031919B1 (https=)
JP (1) JP6014805B2 (https=)
KR (1) KR101685712B1 (https=)
CN (2) CN104673784A (https=)
AU (1) AU2008282101A1 (https=)
BR (1) BRPI0815046A2 (https=)
CA (1) CA2693102C (https=)
DK (1) DK3031919T3 (https=)
EA (1) EA020657B1 (https=)
ES (1) ES2701883T3 (https=)
IL (1) IL202839A0 (https=)
MX (1) MX353478B (https=)
NZ (1) NZ582876A (https=)
WO (1) WO2009018449A1 (https=)
ZA (1) ZA201000152B (https=)

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US9096871B2 (en) 2010-10-06 2015-08-04 Bp Corporation North America Inc. Variant CBH I polypeptides with reduced product inhibition
WO2014070856A2 (en) 2012-11-02 2014-05-08 Bp Corporation North America Inc. Thermotolerant beta-glucosidase variants
GB201308828D0 (en) 2013-03-12 2013-07-03 Verenium Corp Phytase
GB201308843D0 (en) 2013-03-14 2013-07-03 Verenium Corp Phytase formulation
EP3027762A4 (en) 2013-07-25 2017-06-28 BASF Enzymes LLC Phytase
WO2018183612A1 (en) * 2017-03-29 2018-10-04 Editas Medicine, Inc. Nucleic acid mutagenesis methods
MX2020004318A (es) 2017-10-25 2020-08-13 Basf Se Enzimas beta-amilasa.
EP3728620A4 (en) * 2017-12-20 2021-11-03 Basf Se GENE SITE SATURATION MUTAGENESIS (GSSM) PROCESS
EP3728574A1 (en) 2017-12-20 2020-10-28 Basf Se Nucleases and methods for making and using them
US11732250B2 (en) 2018-04-26 2023-08-22 Basf Se Lipase enzymes
CN112384616B (zh) 2018-05-29 2024-09-03 巴斯夫欧洲公司 淀粉酶
US12460221B2 (en) 2018-09-07 2025-11-04 Basf Plant Science Company Gmbh Method for the production of high levels of PUFA in plants
CA3110656A1 (en) 2018-09-07 2020-03-12 Basf Plant Science Company Gmbh Improved method for the production of high levels of pufa in plants
EP3847264A1 (en) 2018-09-07 2021-07-14 Basf Plant Science Company GmbH Improved method for the production of high levels of pufa in plants
US20220186200A1 (en) 2019-03-11 2022-06-16 Basf Se Amylases and methods for making and using them
CA3176520A1 (en) 2019-04-23 2020-10-29 Basf Se Beta-amylase variants
US20230041211A1 (en) 2019-12-20 2023-02-09 Basf Se Decreasing toxicity of terpenes and increasing the production potential in micro-organisms
BR112022024475A2 (pt) 2020-06-04 2022-12-27 Isobionics B V Beta santaleno sintase sintética, ácido nucleico sintético, cassete de expressão, método para produzir uma composição, célula hospedeira não humana, composição, e, uso de qualquer uma das santaleno sintases sintéticas
EP4015626A1 (en) 2020-12-18 2022-06-22 Isobionics B.V. Enzymes and methods for fermentative production of monoterpene esters
JP2024528957A (ja) 2021-08-02 2024-08-01 ビーエーエスエフ ソシエタス・ヨーロピア イオニリデンエタンシンターゼによる新規な芳香化合物の生成

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ATE161541T1 (de) * 1993-06-25 1998-01-15 Univ Yale Chemische-modifizierte oligonukleotide für site- spezifisches mutagenesis
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US6165793A (en) * 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
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Also Published As

Publication number Publication date
EP2183378A4 (en) 2010-09-22
DK3031919T3 (en) 2019-01-07
JP2010535034A (ja) 2010-11-18
EA020657B1 (ru) 2014-12-30
BRPI0815046A2 (pt) 2014-10-14
MX2010001182A (es) 2010-04-21
EP2183378A1 (en) 2010-05-12
US9476078B2 (en) 2016-10-25
KR101685712B1 (ko) 2016-12-12
US20100216192A1 (en) 2010-08-26
EA201070206A1 (ru) 2010-06-30
CA2693102A1 (en) 2009-02-05
ZA201000152B (en) 2011-06-29
CN101821402A (zh) 2010-09-01
WO2009018449A1 (en) 2009-02-05
NZ582876A (en) 2012-07-27
ES2701883T3 (es) 2019-02-26
IL202839A0 (en) 2010-06-30
CN104673784A (zh) 2015-06-03
KR20100045494A (ko) 2010-05-03
EP3031919A1 (en) 2016-06-15
MX353478B (es) 2018-01-15
CN101821402B (zh) 2015-03-04
JP6014805B2 (ja) 2016-10-26
AU2008282101A1 (en) 2009-02-05
EP3031919B1 (en) 2018-09-19

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