EA020657B1 - Специализированная многосайтовая комбинаторная сборка - Google Patents
Специализированная многосайтовая комбинаторная сборка Download PDFInfo
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- EA020657B1 EA020657B1 EA201070206A EA201070206A EA020657B1 EA 020657 B1 EA020657 B1 EA 020657B1 EA 201070206 A EA201070206 A EA 201070206A EA 201070206 A EA201070206 A EA 201070206A EA 020657 B1 EA020657 B1 EA 020657B1
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- C12N15/102—Mutagenizing nucleic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
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- C12N15/102—Mutagenizing nucleic acids
- C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
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| BR112022024475A2 (pt) | 2020-06-04 | 2022-12-27 | Isobionics B V | Beta santaleno sintase sintética, ácido nucleico sintético, cassete de expressão, método para produzir uma composição, célula hospedeira não humana, composição, e, uso de qualquer uma das santaleno sintases sintéticas |
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| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US5849482A (en) | 1988-09-28 | 1998-12-15 | Epoch Pharmaceuticals, Inc. | Crosslinking oligonucleotides |
| ATE414768T1 (de) * | 1991-04-10 | 2008-12-15 | Scripps Research Inst | Bibliotheken heterodimerer rezeptoren mittels phagemiden |
| JP3398957B2 (ja) | 1991-12-24 | 2003-04-21 | ザ・プレジデント・アンド・フエローズ・オブ・ハーバード・カレツジ | Ｄｎａの特定部位の突然変異誘発 |
| US20030194807A1 (en) * | 1992-11-02 | 2003-10-16 | Roberto Crea | Walk-through mutagenesis |
| ATE161541T1 (de) * | 1993-06-25 | 1998-01-15 | Univ Yale | Chemische-modifizierte oligonukleotide für site- spezifisches mutagenesis |
| US6335160B1 (en) * | 1995-02-17 | 2002-01-01 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
| US6165793A (en) * | 1996-03-25 | 2000-12-26 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US5965408A (en) * | 1996-07-09 | 1999-10-12 | Diversa Corporation | Method of DNA reassembly by interrupting synthesis |
| US5830696A (en) * | 1996-12-05 | 1998-11-03 | Diversa Corporation | Directed evolution of thermophilic enzymes |
| US6238884B1 (en) * | 1995-12-07 | 2001-05-29 | Diversa Corporation | End selection in directed evolution |
| US20040023327A1 (en) * | 1995-12-07 | 2004-02-05 | Short Jay M. | End selection in directed evolution |
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| US6713279B1 (en) * | 1995-12-07 | 2004-03-30 | Diversa Corporation | Non-stochastic generation of genetic vaccines and enzymes |
| US6764835B2 (en) * | 1995-12-07 | 2004-07-20 | Diversa Corporation | Saturation mutageneis in directed evolution |
| US6939689B2 (en) * | 1995-12-07 | 2005-09-06 | Diversa Corporation | Exonuclease-mediated nucleic acid reassembly in directed evolution |
| US6562594B1 (en) * | 1999-09-29 | 2003-05-13 | Diversa Corporation | Saturation mutagenesis in directed evolution |
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| US6361974B1 (en) * | 1995-12-07 | 2002-03-26 | Diversa Corporation | Exonuclease-mediated nucleic acid reassembly in directed evolution |
| US20020028443A1 (en) * | 1999-09-27 | 2002-03-07 | Jay M. Short | Method of dna shuffling with polynucleotides produced by blocking or interrupting a synthesis or amplification process |
| US5789166A (en) * | 1995-12-08 | 1998-08-04 | Stratagene | Circular site-directed mutagenesis |
| GB9701425D0 (en) * | 1997-01-24 | 1997-03-12 | Bioinvent Int Ab | A method for in vitro molecular evolution of protein function |
| DE69940879D1 (de) * | 1998-11-11 | 2009-06-25 | Japan Science & Tech Agency | Verfahren zur mutagenese |
| US6376191B1 (en) * | 2000-03-22 | 2002-04-23 | Mergen, Ltd. | Microarray-based analysis of polynucleotide sequence variations |
| JP3859947B2 (ja) * | 2000-08-04 | 2006-12-20 | 独立行政法人理化学研究所 | 突然変異導入方法 |
| WO2002014490A2 (en) * | 2000-08-11 | 2002-02-21 | Genencor International, Inc. | Bacillus transformation, transformants and mutant libraries |
| FR2813314B1 (fr) * | 2000-08-25 | 2004-05-07 | Biomethodes | Procede de mutagenese dirigee massive |
| USPP13969P3 (en) | 2001-04-05 | 2003-07-15 | Her Majesty The Queen In Right Of Canada As Represented By The Minister Of Agriculture | Shrub rose plant named ‘Morden Sunrise’ |
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| DE10148607A1 (de) * | 2001-10-02 | 2003-04-10 | Max Planck Gesellschaft | Methoden zur DNA-Manipulation |
| WO2003040376A1 (en) * | 2001-11-02 | 2003-05-15 | Olga Makarova | Method for site-directed mutagenesis of nucleic acid molecules using a single primer |
| CA2468710A1 (en) * | 2001-12-03 | 2003-06-12 | Diversa Corporation | Chromosomal saturation mutagenesis |
| AUPS263402A0 (en) * | 2002-05-29 | 2002-06-20 | Young, Lei | Targeted amplification of template strand (TATS) technology, and its use in DNA mutagenesis, deletion & insertion |
| US20060134624A1 (en) * | 2003-02-06 | 2006-06-22 | Salerno John C | Polymerase-based protocols for the introductions of deletions and insertions |
| US20060228786A1 (en) | 2003-02-06 | 2006-10-12 | Salerno John C | Polymerase-based protocols for the introduction of deletions and insertions |
| US6878531B1 (en) * | 2003-11-10 | 2005-04-12 | Medical College Of Georgia Research Institute | Method for multiple site-directed mutagenesis |
| US20060288786A1 (en) * | 2005-03-18 | 2006-12-28 | Flores Andres H L | Ultrasonically coupled scanning probe microscope |
| US20060223066A1 (en) * | 2005-03-29 | 2006-10-05 | Applera Corporation | Methods for normalizing and for identifying small nucleic acids |
| EP1907584A2 (en) * | 2005-06-28 | 2008-04-09 | Agencourt Personal Genomics Corporation | Methods of producing and sequencing modified polynucleotides |
-
2008
- 2008-07-31 CA CA2693102A patent/CA2693102C/en active Active
- 2008-07-31 EP EP16154206.3A patent/EP3031919B1/en active Active
- 2008-07-31 CN CN201510050333.XA patent/CN104673784A/zh active Pending
- 2008-07-31 NZ NZ582876A patent/NZ582876A/en not_active IP Right Cessation
- 2008-07-31 MX MX2010001182A patent/MX353478B/es active IP Right Grant
- 2008-07-31 WO PCT/US2008/071771 patent/WO2009018449A1/en not_active Ceased
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- 2008-07-31 EP EP08782567A patent/EP2183378A4/en not_active Ceased
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- 2008-07-31 BR BRPI0815046-0A2A patent/BRPI0815046A2/pt not_active Application Discontinuation
- 2008-07-31 AU AU2008282101A patent/AU2008282101A1/en not_active Abandoned
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Also Published As
| Publication number | Publication date |
|---|---|
| EP2183378A4 (en) | 2010-09-22 |
| DK3031919T3 (en) | 2019-01-07 |
| JP2010535034A (ja) | 2010-11-18 |
| BRPI0815046A2 (pt) | 2014-10-14 |
| MX2010001182A (es) | 2010-04-21 |
| EP2183378A1 (en) | 2010-05-12 |
| US9476078B2 (en) | 2016-10-25 |
| KR101685712B1 (ko) | 2016-12-12 |
| US20100216192A1 (en) | 2010-08-26 |
| EA201070206A1 (ru) | 2010-06-30 |
| CA2693102A1 (en) | 2009-02-05 |
| ZA201000152B (en) | 2011-06-29 |
| CN101821402A (zh) | 2010-09-01 |
| WO2009018449A1 (en) | 2009-02-05 |
| NZ582876A (en) | 2012-07-27 |
| ES2701883T3 (es) | 2019-02-26 |
| IL202839A0 (en) | 2010-06-30 |
| CN104673784A (zh) | 2015-06-03 |
| KR20100045494A (ko) | 2010-05-03 |
| EP3031919A1 (en) | 2016-06-15 |
| MX353478B (es) | 2018-01-15 |
| CN101821402B (zh) | 2015-03-04 |
| JP6014805B2 (ja) | 2016-10-26 |
| AU2008282101A1 (en) | 2009-02-05 |
| CA2693102C (en) | 2020-03-10 |
| EP3031919B1 (en) | 2018-09-19 |
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