CA2683977C - A method of norovirus virus-like particle purification comprising ion exchange chromatography - Google Patents

A method of norovirus virus-like particle purification comprising ion exchange chromatography Download PDF

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Publication number
CA2683977C
CA2683977C CA2683977A CA2683977A CA2683977C CA 2683977 C CA2683977 C CA 2683977C CA 2683977 A CA2683977 A CA 2683977A CA 2683977 A CA2683977 A CA 2683977A CA 2683977 C CA2683977 C CA 2683977C
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Prior art keywords
vlps
chromatographic
chromatographic material
vlp
chromatography
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CA2683977A
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English (en)
French (fr)
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CA2683977A1 (en
Inventor
Thomas S. Vedvick
Bryan Steadman
Charles Richardson
Thomas R. Foubert
Charles R. Petrie
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Takeda Vaccines Inc
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Takeda Vaccines Inc
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Publication of CA2683977A1 publication Critical patent/CA2683977A1/en
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/20—Pills, tablets, discs, rods
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/02—Recovery or purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/16011—Caliciviridae
    • C12N2770/16023—Virus like particles [VLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/16011—Caliciviridae
    • C12N2770/16034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/16011—Caliciviridae
    • C12N2770/16051—Methods of production or purification of viral material

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Epidemiology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA2683977A 2007-03-14 2008-03-14 A method of norovirus virus-like particle purification comprising ion exchange chromatography Active CA2683977C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US90682107P 2007-03-14 2007-03-14
US60/906,821 2007-03-14
PCT/US2008/057072 WO2008113011A2 (en) 2007-03-14 2008-03-14 Virus like particle purification

Publications (2)

Publication Number Publication Date
CA2683977A1 CA2683977A1 (en) 2008-09-18
CA2683977C true CA2683977C (en) 2017-04-25

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Family Applications (1)

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CA2683977A Active CA2683977C (en) 2007-03-14 2008-03-14 A method of norovirus virus-like particle purification comprising ion exchange chromatography

Country Status (12)

Country Link
US (4) US8481693B2 (OSRAM)
EP (1) EP2134360B1 (OSRAM)
JP (4) JP5284290B2 (OSRAM)
KR (3) KR20150098681A (OSRAM)
AU (1) AU2008224877B2 (OSRAM)
CA (1) CA2683977C (OSRAM)
DK (1) DK2134360T3 (OSRAM)
ES (1) ES2559421T3 (OSRAM)
HU (1) HUE028605T2 (OSRAM)
PL (1) PL2134360T3 (OSRAM)
SG (2) SG179488A1 (OSRAM)
WO (1) WO2008113011A2 (OSRAM)

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Also Published As

Publication number Publication date
KR20170001720A (ko) 2017-01-04
AU2008224877B2 (en) 2013-07-11
KR20100015562A (ko) 2010-02-12
JP2010530734A (ja) 2010-09-16
US20160317645A1 (en) 2016-11-03
EP2134360B1 (en) 2015-11-18
EP2134360A2 (en) 2009-12-23
SG10201701988TA (en) 2017-04-27
WO2008113011A3 (en) 2008-12-24
JP2017086090A (ja) 2017-05-25
JP5284290B2 (ja) 2013-09-11
CA2683977A1 (en) 2008-09-18
EP2134360A4 (en) 2011-08-24
HK1138795A1 (zh) 2010-09-03
US8481693B2 (en) 2013-07-09
US9359410B2 (en) 2016-06-07
WO2008113011A2 (en) 2008-09-18
SG179488A1 (en) 2012-04-27
ES2559421T3 (es) 2016-02-12
KR101576219B1 (ko) 2015-12-10
US10792353B2 (en) 2020-10-06
JP2013176392A (ja) 2013-09-09
KR20150098681A (ko) 2015-08-28
US20190282686A1 (en) 2019-09-19
DK2134360T3 (en) 2016-02-22
US10172930B2 (en) 2019-01-08
PL2134360T3 (pl) 2016-05-31
AU2008224877A1 (en) 2008-09-18
US20130344107A1 (en) 2013-12-26
JP2019068846A (ja) 2019-05-09
US20100150961A1 (en) 2010-06-17
HUE028605T2 (en) 2016-12-28

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