CA2635380C - Polypeptide having esterase activity and recombinant esterase and use thereof - Google Patents
Polypeptide having esterase activity and recombinant esterase and use thereof Download PDFInfo
- Publication number
- CA2635380C CA2635380C CA2635380A CA2635380A CA2635380C CA 2635380 C CA2635380 C CA 2635380C CA 2635380 A CA2635380 A CA 2635380A CA 2635380 A CA2635380 A CA 2635380A CA 2635380 C CA2635380 C CA 2635380C
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- CA
- Canada
- Prior art keywords
- alkyl
- halopent
- polypeptide
- formula
- esterase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 230000000694 effects Effects 0.000 title claims abstract description 34
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 23
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- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 23
- 125000003275 alpha amino acid group Chemical group 0.000 claims abstract description 14
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- 125000004108 n-butyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
- 125000004123 n-propyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
- VGKONPUVOVVNSU-UHFFFAOYSA-N naphthalen-1-yl acetate Chemical compound C1=CC=C2C(OC(=O)C)=CC=CC2=C1 VGKONPUVOVVNSU-UHFFFAOYSA-N 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 125000001147 pentyl group Chemical group C(CCCC)* 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 229940127557 pharmaceutical product Drugs 0.000 description 1
- 229960003531 phenolsulfonphthalein Drugs 0.000 description 1
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000002708 random mutagenesis Methods 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 125000000999 tert-butyl group Chemical group [H]C([H])([H])C(*)(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000008096 xylene Substances 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
- 239000007222 ypd medium Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
- C12P41/003—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by ester formation, lactone formation or the inverse reactions
- C12P41/005—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by ester formation, lactone formation or the inverse reactions by esterification of carboxylic acid groups in the enantiomers or the inverse reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ATA2081/2005 | 2005-12-27 | ||
| AT0208105A AT502990A1 (de) | 2005-12-27 | 2005-12-27 | Neues polypeptid mit esteraseaktivität, und rekombinante esterase sowie deren verwendung |
| PCT/EP2006/011832 WO2007073845A2 (en) | 2005-12-27 | 2006-12-08 | Polypeptide having esterase activity and recombinant esterase and use thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2635380A1 CA2635380A1 (en) | 2007-07-05 |
| CA2635380C true CA2635380C (en) | 2014-10-21 |
Family
ID=38017096
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2635380A Active CA2635380C (en) | 2005-12-27 | 2006-12-08 | Polypeptide having esterase activity and recombinant esterase and use thereof |
Country Status (14)
| Country | Link |
|---|---|
| US (2) | US8158393B2 (enExample) |
| EP (1) | EP1966373B1 (enExample) |
| JP (1) | JP5119166B2 (enExample) |
| KR (1) | KR101388391B1 (enExample) |
| CN (1) | CN101351548B (enExample) |
| AT (2) | AT502990A1 (enExample) |
| BR (1) | BRPI0621280A8 (enExample) |
| CA (1) | CA2635380C (enExample) |
| ES (1) | ES2383702T3 (enExample) |
| IL (1) | IL192400A (enExample) |
| NO (1) | NO343225B1 (enExample) |
| PL (1) | PL1966373T3 (enExample) |
| SG (1) | SG170757A1 (enExample) |
| WO (1) | WO2007073845A2 (enExample) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PL2171061T3 (pl) * | 2007-07-04 | 2019-03-29 | Patheon Austria Gmbh & Co Kg | Preparat esterazy |
| ES2689941T3 (es) * | 2009-04-24 | 2018-11-16 | Patheon Austria Gmbh & Co Kg | Esterasas de hígado de cerdo mejoradas |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5817490A (en) * | 1996-05-17 | 1998-10-06 | Eastman Chemical Company | Enzymatic process for the manufacture of ascorbic acid 2-keto-L-gulonic acid and esters of 2-keto-L-gulonic acid |
| DE10061864A1 (de) | 2000-12-12 | 2002-07-11 | Aventis Res & Tech Gmbh & Co | Rekombinante Schweineleberesterase, deren Verwendung sowie ein Verfahren zu deren Herstellung |
| DE10258327A1 (de) * | 2002-12-13 | 2004-06-24 | Degussa Ag | Artifizielle Esterasen |
-
2005
- 2005-12-27 AT AT0208105A patent/AT502990A1/de not_active Application Discontinuation
-
2006
- 2006-12-08 US US12/159,547 patent/US8158393B2/en not_active Expired - Fee Related
- 2006-12-08 KR KR1020087018341A patent/KR101388391B1/ko not_active Expired - Fee Related
- 2006-12-08 SG SG201102064-1A patent/SG170757A1/en unknown
- 2006-12-08 EP EP06829433A patent/EP1966373B1/en not_active Not-in-force
- 2006-12-08 PL PL06829433T patent/PL1966373T3/pl unknown
- 2006-12-08 BR BRPI0621280A patent/BRPI0621280A8/pt not_active Application Discontinuation
- 2006-12-08 WO PCT/EP2006/011832 patent/WO2007073845A2/en not_active Ceased
- 2006-12-08 AT AT06829433T patent/ATE549400T1/de active
- 2006-12-08 CN CN200680049665.5A patent/CN101351548B/zh not_active Expired - Fee Related
- 2006-12-08 ES ES06829433T patent/ES2383702T3/es active Active
- 2006-12-08 CA CA2635380A patent/CA2635380C/en active Active
- 2006-12-08 JP JP2008547868A patent/JP5119166B2/ja not_active Expired - Fee Related
-
2008
- 2008-06-23 IL IL192400A patent/IL192400A/en active IP Right Grant
- 2008-06-27 NO NO20082864A patent/NO343225B1/no not_active IP Right Cessation
-
2012
- 2012-03-12 US US13/417,734 patent/US8642312B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| KR101388391B1 (ko) | 2014-04-22 |
| JP5119166B2 (ja) | 2013-01-16 |
| US20120220014A1 (en) | 2012-08-30 |
| CN101351548B (zh) | 2015-08-19 |
| WO2007073845A8 (en) | 2008-07-24 |
| AT502990A1 (de) | 2007-07-15 |
| JP2009521239A (ja) | 2009-06-04 |
| NO343225B1 (no) | 2018-12-10 |
| EP1966373A2 (en) | 2008-09-10 |
| CN101351548A (zh) | 2009-01-21 |
| BRPI0621280A2 (pt) | 2011-12-06 |
| IL192400A0 (en) | 2009-02-11 |
| US20100151541A1 (en) | 2010-06-17 |
| US8158393B2 (en) | 2012-04-17 |
| CA2635380A1 (en) | 2007-07-05 |
| BRPI0621280A8 (pt) | 2017-09-19 |
| SG170757A1 (en) | 2011-05-30 |
| KR20080093109A (ko) | 2008-10-20 |
| NO20082864L (no) | 2008-09-25 |
| PL1966373T3 (pl) | 2012-08-31 |
| WO2007073845A3 (en) | 2007-08-02 |
| ES2383702T3 (es) | 2012-06-25 |
| WO2007073845A2 (en) | 2007-07-05 |
| IL192400A (en) | 2013-06-27 |
| HK1128306A1 (en) | 2009-10-23 |
| US8642312B2 (en) | 2014-02-04 |
| ATE549400T1 (de) | 2012-03-15 |
| EP1966373B1 (en) | 2012-03-14 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |