CN101351548B - 具有酯酶活性的多肽和重组的酯酶及其用途 - Google Patents

具有酯酶活性的多肽和重组的酯酶及其用途 Download PDF

Info

Publication number
CN101351548B
CN101351548B CN200680049665.5A CN200680049665A CN101351548B CN 101351548 B CN101351548 B CN 101351548B CN 200680049665 A CN200680049665 A CN 200680049665A CN 101351548 B CN101351548 B CN 101351548B
Authority
CN
China
Prior art keywords
alkyl
formula
leu
gly
ala
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN200680049665.5A
Other languages
English (en)
Chinese (zh)
Other versions
CN101351548A (zh
Inventor
格哈德·斯坦保尔
迈克尔·施塔内克
彼得·波亚尔里尔
沃夫冈·斯卡安可
赫尔马特·施瓦布
马塞尔·乌博尔特斯
乔纳斯·西基尔科斯
哈拉德·皮希勒
马诺拉·埃尔曼
克里斯托弗·赞兹马尔
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DSM IP Assets BV
Original Assignee
DSM Fine Chemicals Austria Nfg GmbH and Co KG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DSM Fine Chemicals Austria Nfg GmbH and Co KG filed Critical DSM Fine Chemicals Austria Nfg GmbH and Co KG
Publication of CN101351548A publication Critical patent/CN101351548A/zh
Application granted granted Critical
Publication of CN101351548B publication Critical patent/CN101351548B/zh
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
    • C12P41/003—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by ester formation, lactone formation or the inverse reactions
    • C12P41/005—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by ester formation, lactone formation or the inverse reactions by esterification of carboxylic acid groups in the enantiomers or the inverse reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN200680049665.5A 2005-12-27 2006-12-08 具有酯酶活性的多肽和重组的酯酶及其用途 Expired - Fee Related CN101351548B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
ATA2081/2005 2005-12-27
AT0208105A AT502990A1 (de) 2005-12-27 2005-12-27 Neues polypeptid mit esteraseaktivität, und rekombinante esterase sowie deren verwendung
PCT/EP2006/011832 WO2007073845A2 (en) 2005-12-27 2006-12-08 Polypeptide having esterase activity and recombinant esterase and use thereof

Publications (2)

Publication Number Publication Date
CN101351548A CN101351548A (zh) 2009-01-21
CN101351548B true CN101351548B (zh) 2015-08-19

Family

ID=38017096

Family Applications (1)

Application Number Title Priority Date Filing Date
CN200680049665.5A Expired - Fee Related CN101351548B (zh) 2005-12-27 2006-12-08 具有酯酶活性的多肽和重组的酯酶及其用途

Country Status (14)

Country Link
US (2) US8158393B2 (enExample)
EP (1) EP1966373B1 (enExample)
JP (1) JP5119166B2 (enExample)
KR (1) KR101388391B1 (enExample)
CN (1) CN101351548B (enExample)
AT (2) AT502990A1 (enExample)
BR (1) BRPI0621280A8 (enExample)
CA (1) CA2635380C (enExample)
ES (1) ES2383702T3 (enExample)
IL (1) IL192400A (enExample)
NO (1) NO343225B1 (enExample)
PL (1) PL1966373T3 (enExample)
SG (1) SG170757A1 (enExample)
WO (1) WO2007073845A2 (enExample)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL2171061T3 (pl) * 2007-07-04 2019-03-29 Patheon Austria Gmbh & Co Kg Preparat esterazy
ES2689941T3 (es) * 2009-04-24 2018-11-16 Patheon Austria Gmbh & Co Kg Esterasas de hígado de cerdo mejoradas

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004055177A2 (en) * 2002-12-13 2004-07-01 Degussa Ag Artificial esterases

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5817490A (en) * 1996-05-17 1998-10-06 Eastman Chemical Company Enzymatic process for the manufacture of ascorbic acid 2-keto-L-gulonic acid and esters of 2-keto-L-gulonic acid
DE10061864A1 (de) 2000-12-12 2002-07-11 Aventis Res & Tech Gmbh & Co Rekombinante Schweineleberesterase, deren Verwendung sowie ein Verfahren zu deren Herstellung

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004055177A2 (en) * 2002-12-13 2004-07-01 Degussa Ag Artificial esterases

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DAVID LAETITIA ET AL.Purification and molecular cloning of porcine intestinal glycerol-ester hydrolase: Evidence for its identity with carboxylesterase.《 EUROPEAN JOURNAL OFBIOCHEMISTRY》.1998,第257卷(第1期),142-148. *
LANGE S ET AL.Cloning, functional expression, and characterization of recombinant pig liver esterase.《 CHEMBIOCHEM : A EUROPEAN JOURNAL OF CHEMICAL BIOLOGY》.2001,第2卷(第7-8期),1439-4227. *
TOONE E J ET A.ENZYMES IN ORGANIC SYNTHESIS 49. RESOLUTIONS OF RACEMIC MONOCYCLIC ESTERS WITH PIG LIVER ESTERASE.《TETRAHEDRON ASYMMETRY》.1991,第2卷(第3期),207-222. *

Also Published As

Publication number Publication date
KR101388391B1 (ko) 2014-04-22
JP5119166B2 (ja) 2013-01-16
US20120220014A1 (en) 2012-08-30
WO2007073845A8 (en) 2008-07-24
AT502990A1 (de) 2007-07-15
JP2009521239A (ja) 2009-06-04
NO343225B1 (no) 2018-12-10
EP1966373A2 (en) 2008-09-10
CN101351548A (zh) 2009-01-21
BRPI0621280A2 (pt) 2011-12-06
IL192400A0 (en) 2009-02-11
US20100151541A1 (en) 2010-06-17
CA2635380C (en) 2014-10-21
US8158393B2 (en) 2012-04-17
CA2635380A1 (en) 2007-07-05
BRPI0621280A8 (pt) 2017-09-19
SG170757A1 (en) 2011-05-30
KR20080093109A (ko) 2008-10-20
NO20082864L (no) 2008-09-25
PL1966373T3 (pl) 2012-08-31
WO2007073845A3 (en) 2007-08-02
ES2383702T3 (es) 2012-06-25
WO2007073845A2 (en) 2007-07-05
IL192400A (en) 2013-06-27
HK1128306A1 (en) 2009-10-23
US8642312B2 (en) 2014-02-04
ATE549400T1 (de) 2012-03-15
EP1966373B1 (en) 2012-03-14

Similar Documents

Publication Publication Date Title
Lange et al. Cloning, functional expression, and characterization of recombinant pig liver esterase
US8945887B2 (en) Whole cell biocatalyst
Heim et al. Functional expression of a mammalian acetylcholinesterase in Pichia pastoris: comparison to acetylcholinesterase, expressed and reconstituted from Escherichia coli
DK2013336T3 (en) New group of esterases for the production of fine and specialty chemicals
CN101351548B (zh) 具有酯酶活性的多肽和重组的酯酶及其用途
JP4263482B2 (ja) 組み換えブタ肝臓エステラーゼ、その使用及びその製造方法
CN101641437B (zh) 猪肝酯酶的同工型
KR20050074313A (ko) 세팔로스포린 ｃ 아실라제
US20100112662A1 (en) Microorganism for producing recombinant pig liver esterase
JP2009521239A5 (enExample)
HK1128306B (en) Polypeptide having esterase activity and ecombinant esterase and use thereof
WO2007073847A1 (en) Novel polypeptide having esterase activity and recombinant esterase and use thereof
AT502992B1 (de) Verfahren zur herstellung von enantiomerenangereicherten 2-alkyl-5-halogen-pent-4-en-carbonsäuren bzw. -carbonsäureestern
KR100427409B1 (ko) 신규 에스터라제, 이의 대량 생산방법 및 이 효소를이용한 광학활성 아릴프로피온산을 고효율로 생산하는 방법
CN106148300A (zh) 一种酯酶est112‑2及其编码基因和应用

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 1128306

Country of ref document: HK

C14 Grant of patent or utility model
GR01 Patent grant
REG Reference to a national code

Ref country code: HK

Ref legal event code: GR

Ref document number: 1128306

Country of ref document: HK

C41 Transfer of patent application or patent right or utility model
TR01 Transfer of patent right

Effective date of registration: 20160311

Address after: Linz, Austria

Patentee after: DSM IP ASSETS B.V.

Address before: Linz, Austria

Patentee before: DSM FINE CHEMICALS AUSTRIA NFG GmbH & Co.KG

CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20150819

CF01 Termination of patent right due to non-payment of annual fee