CA2577180A1 - Single protein production in living cells facilitated by a messenger rna interferase - Google Patents

Single protein production in living cells facilitated by a messenger rna interferase Download PDF

Info

Publication number
CA2577180A1
CA2577180A1 CA002577180A CA2577180A CA2577180A1 CA 2577180 A1 CA2577180 A1 CA 2577180A1 CA 002577180 A CA002577180 A CA 002577180A CA 2577180 A CA2577180 A CA 2577180A CA 2577180 A1 CA2577180 A1 CA 2577180A1
Authority
CA
Canada
Prior art keywords
mutated
acid sequence
nucleic acid
target protein
mrna interferase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002577180A
Other languages
English (en)
French (fr)
Inventor
Inouye Masayori
Junjie Zhang
Motoo Suzuki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Medicine and Dentistry of New Jersey
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2577180A1 publication Critical patent/CA2577180A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10Cells modified by introduction of foreign genetic material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases RNAses, DNAses
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/06Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA002577180A 2004-11-04 2005-11-04 Single protein production in living cells facilitated by a messenger rna interferase Abandoned CA2577180A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US62497604P 2004-11-04 2004-11-04
US60/624,976 2004-11-04
PCT/US2005/040107 WO2006055292A2 (en) 2004-11-04 2005-11-04 Single protein production in living cells facilitated by a messenger rna interferase

Publications (1)

Publication Number Publication Date
CA2577180A1 true CA2577180A1 (en) 2006-05-26

Family

ID=36407606

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002577180A Abandoned CA2577180A1 (en) 2004-11-04 2005-11-04 Single protein production in living cells facilitated by a messenger rna interferase

Country Status (6)

Country Link
EP (1) EP1812582A4 (zh)
JP (1) JP5013375B2 (zh)
KR (1) KR101064783B1 (zh)
CN (1) CN101052713B (zh)
CA (1) CA2577180A1 (zh)
WO (1) WO2006055292A2 (zh)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007020873A1 (ja) * 2005-08-16 2007-02-22 Takara Bio Inc. 免疫不全ウイルス感染症の治療または予防のための核酸
WO2010040134A2 (en) 2008-10-04 2010-04-08 University Of Medicine And Dentistry Of New Jersey Independently inducible system of gene expression
EP2848695A1 (en) * 2013-09-16 2015-03-18 Inria Institut National de Recherche en Informatique et en Automatique Method for producing metabolites, peptides and recombinant proteins
CN103484471B (zh) * 2013-09-30 2015-07-08 王悦 人表皮细胞生长因子核酸序列及大肠杆菌表达载体
KR101776368B1 (ko) * 2014-10-02 2017-09-07 서울시립대학교 산학협력단 전령 rna 나노입자 및 그 제조방법
TW202000240A (zh) * 2018-02-23 2020-01-01 日商盧卡科學股份有限公司 用於在粒線體內使蛋白質表現之核酸、封入前述核酸之脂質膜結構體及其等之用途

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5807718A (en) * 1994-12-02 1998-09-15 The Scripps Research Institute Enzymatic DNA molecules
CN103937827A (zh) * 2003-06-13 2014-07-23 新泽西内科与牙科大学 Rna干扰酶及其使用方法

Also Published As

Publication number Publication date
KR101064783B1 (ko) 2011-09-14
JP2008518623A (ja) 2008-06-05
WO2006055292A3 (en) 2006-08-03
KR20080088350A (ko) 2008-10-02
EP1812582A4 (en) 2013-03-20
WO2006055292A2 (en) 2006-05-26
CN101052713A (zh) 2007-10-10
CN101052713B (zh) 2011-01-26
JP5013375B2 (ja) 2012-08-29
EP1812582A2 (en) 2007-08-01

Similar Documents

Publication Publication Date Title
JP7308160B2 (ja) メチロトローフ酵母の遺伝子操作の発現構築物および方法
WO2007137144A2 (en) Single protein production in living cells facilitated by a messenger rna interferase
US11427932B2 (en) Materials and methods for protein production
CA2577180A1 (en) Single protein production in living cells facilitated by a messenger rna interferase
CN113564171B (zh) 一种提高多肽可溶性表达产量的方法
US7985575B2 (en) Single protein production in living cells facilitated by a messenger RNA interferase
Linova et al. A novel approach for production of an active N-terminally truncated Ulp1 (SUMO protease 1) catalytic domain from Escherichia coli inclusion bodies
JP4857260B2 (ja) 低温性微生物由来エンドヌクレアーゼ
Chandler et al. At UBP3 and At UBP4 are two closely related Arabidopsis thaliana ubiquitin-specific proteases present in the nucleus
Hennessy et al. Rational mutagenesis of a 40 kDa heat shock protein from Agrobacterium tumefaciens identifies amino acid residues critical to its in vivo function
Klanner et al. MAP-1 and IAP-1, two novel AAA proteases with catalytic sites on opposite membrane surfaces in mitochondrial inner membrane of Neurospora crassa
JP6991423B2 (ja) グルコースオキシダーゼCnGODA及びその遺伝子ならびに使用
JP3549210B2 (ja) プラスミド
CN110951711B (zh) 一种具有降解手性酯活性的酯酶及其编码基因和应用
JP4974891B2 (ja) 新規なエンドリボヌクレアーゼ
EP1468284B1 (en) Method for measuring intracellular atp and/or gene expression
JP5240970B2 (ja) 界面活性剤存在下で安定なコレステロールオキシダーゼ
US20090259035A1 (en) Method for producing recombinant RNase A
JP4714848B2 (ja) Dnaポリメラーゼ変異体
JPWO2007010740A1 (ja) 新規なエンドリボヌクレアーゼ
JP2001103972A (ja) ルシフェリンを再生する能力を有するタンパク質をコードする遺伝子、新規な組み換え体dna及びルシフェリンを再生する能力を有するタンパク質の製造法
Kim A microbial D-hydantoinase is stabilized and overexpressed as a catalytically active dimer by truncation and insertion of the C-terminal region
WO2023096513A1 (en) Tritirachium album proteinase k mutant and its zymogen, expression plasmid, recombinant pichia pastoris strain and method of producing the mature form of proteinase k mutant
JP2001161375A (ja) コレステロール・エステラーゼ遺伝子、組み換え体dna及びコレステロール・エステラーゼの製造法
JP2002247985A (ja) Dna修復酵素遺伝子

Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued