JP5013375B2 - メッセンジャーｒｎａ干渉酵素が促進する生細胞における単一タンパク質産生 - Google Patents

メッセンジャーｒｎａ干渉酵素が促進する生細胞における単一タンパク質産生 Download PDF

Info

Publication number
JP5013375B2
JP5013375B2 JP2007540092A JP2007540092A JP5013375B2 JP 5013375 B2 JP5013375 B2 JP 5013375B2 JP 2007540092 A JP2007540092 A JP 2007540092A JP 2007540092 A JP2007540092 A JP 2007540092A JP 5013375 B2 JP5013375 B2 JP 5013375B2
Authority
JP
Japan
Prior art keywords
acid sequence
nucleic acid
mrna
target protein
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2007540092A
Other languages
English (en)
Japanese (ja)
Other versions
JP2008518623A (ja
Inventor
正順 井上
チャン，ジュンジエ
基生 鈴木
Original Assignee
ユニヴァーシティ オブ メディシン アンド デンティストリ オブ ニュージャーシィ
正順 井上
チャン，ジュンジエ
基生 鈴木
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ユニヴァーシティ オブ メディシン アンド デンティストリ オブ ニュージャーシィ, 正順 井上, チャン，ジュンジエ, 基生 鈴木 filed Critical ユニヴァーシティ オブ メディシン アンド デンティストリ オブ ニュージャーシィ
Publication of JP2008518623A publication Critical patent/JP2008518623A/ja
Application granted granted Critical
Publication of JP5013375B2 publication Critical patent/JP5013375B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2007540092A 2004-11-04 2005-11-04 メッセンジャーｒｎａ干渉酵素が促進する生細胞における単一タンパク質産生 Expired - Fee Related JP5013375B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US62497604P 2004-11-04 2004-11-04
US60/624,976 2004-11-04
PCT/US2005/040107 WO2006055292A2 (en) 2004-11-04 2005-11-04 Single protein production in living cells facilitated by a messenger rna interferase

Publications (2)

Publication Number Publication Date
JP2008518623A JP2008518623A (ja) 2008-06-05
JP5013375B2 true JP5013375B2 (ja) 2012-08-29

Family

ID=36407606

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2007540092A Expired - Fee Related JP5013375B2 (ja) 2004-11-04 2005-11-04 メッセンジャーｒｎａ干渉酵素が促進する生細胞における単一タンパク質産生

Country Status (6)

Country Link
EP (1) EP1812582A4 (zh)
JP (1) JP5013375B2 (zh)
KR (1) KR101064783B1 (zh)
CN (1) CN101052713B (zh)
CA (1) CA2577180A1 (zh)
WO (1) WO2006055292A2 (zh)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007020873A1 (ja) * 2005-08-16 2007-02-22 Takara Bio Inc. 免疫不全ウイルス感染症の治療または予防のための核酸
WO2010040134A2 (en) 2008-10-04 2010-04-08 University Of Medicine And Dentistry Of New Jersey Independently inducible system of gene expression
EP2848695A1 (en) * 2013-09-16 2015-03-18 Inria Institut National de Recherche en Informatique et en Automatique Method for producing metabolites, peptides and recombinant proteins
CN103484471B (zh) * 2013-09-30 2015-07-08 王悦 人表皮细胞生长因子核酸序列及大肠杆菌表达载体
KR101776368B1 (ko) * 2014-10-02 2017-09-07 서울시립대학교 산학협력단 전령 ｒｎａ 나노입자 및 그 제조방법
TW202000240A (zh) * 2018-02-23 2020-01-01 日商盧卡科學股份有限公司 用於在粒線體內使蛋白質表現之核酸、封入前述核酸之脂質膜結構體及其等之用途

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5807718A (en) * 1994-12-02 1998-09-15 The Scripps Research Institute Enzymatic DNA molecules
CN103937827A (zh) * 2003-06-13 2014-07-23 新泽西内科与牙科大学 Rna干扰酶及其使用方法

Also Published As

Publication number Publication date
KR101064783B1 (ko) 2011-09-14
JP2008518623A (ja) 2008-06-05
CA2577180A1 (en) 2006-05-26
WO2006055292A3 (en) 2006-08-03
KR20080088350A (ko) 2008-10-02
EP1812582A4 (en) 2013-03-20
WO2006055292A2 (en) 2006-05-26
CN101052713A (zh) 2007-10-10
CN101052713B (zh) 2011-01-26
EP1812582A2 (en) 2007-08-01

Similar Documents

Publication Publication Date Title
EP3460069B1 (en) Therapeutically active compounds and their methods of use
JP5013375B2 (ja) メッセンジャーｒｎａ干渉酵素が促進する生細胞における単一タンパク質産生
Resch et al. Downstream box‐anti‐downstream box interactions are dispensable for translation initiation of leaderless mRNAs.
WO2007137144A2 (en) Single protein production in living cells facilitated by a messenger rna interferase
US20050196774A1 (en) Synthetic genes for enhanced expression
KR20240055073A (ko) 클래스 ii, v형 crispr 시스템
JP2863738B2 (ja) 熱安定性ピロホスファターゼをコードするｄｎａ
US7985575B2 (en) Single protein production in living cells facilitated by a messenger RNA interferase
KR100221385B1 (ko) 스트렙토마이세스 아베르미틸리스로 부터의 측쇄 알파-케토산 데하이드로게나제 복합체를 암호화하는 유전자
JP3549210B2 (ja) プラスミド
Itoh et al. Cloning of soluble alkaline phosphatase cDNA and molecular basis of the polymorphic nature in alkaline phosphatase isozymes of Bombyx mori midgut
JP2003517837A (ja) バクテリオファージファイ６〜ファイ１４由来のｒｎａポリメラーゼ類およびその使用
TWI305230B (en) Nucleic acid construct and expression vector for enhancing the production of recombinant protein, and method for the massive production of recombinant protein
US20220081692A1 (en) Combinatorial Assembly of Composite Arrays of Site-Specific Synthetic Transposons Inserted Into Sequences Comprising Novel Target Sites in Modular Prokaryotic and Eukaryotic Vectors
de Vries et al. Cloning and sequencing of the Proteus mirabilis gene for a single-stranded DNA-binding protein (SSB) and complementation of Escherichia coli ssb point and deletion mutations
US6218145B1 (en) Bacterial expression systems based on plastic or mitochondrial promoter combinations
JP5240970B2 (ja) 界面活性剤存在下で安定なコレステロールオキシダーゼ
WO2003027280A1 (fr) Systeme de surexpression genique
US20090075270A1 (en) Products and Methods Relating to the Use of the Endoribonuclease Kid/PemK
US20090259035A1 (en) Method for producing recombinant RNase A
CN114480345B (zh) MazF突变体、重组载体、重组工程菌及其应用
JP4714848B2 (ja) Ｄｎａポリメラーゼ変異体
US7098022B2 (en) Family 19 class IV chitinase gene from yam
KR20240051994A (ko) 레트로트랜스포존 및 이의 기능적 단편을 포함하는 시스템, 조성물, 및 방법
KR101188144B1 (ko) ｈＡＲＤ1-단백질의 변이체 및 이를 포함하는 융합 단백질

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20081017

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20110509

A521 Written amendment

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20110809

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20110907

A521 Written amendment

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20111207

A711 Notification of change in applicant

Free format text: JAPANESE INTERMEDIATE CODE: A711

Effective date: 20111207

A521 Written amendment

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20111207

A072 Dismissal of procedure [no reply to invitation to correct request for examination]

Free format text: JAPANESE INTERMEDIATE CODE: A073

Effective date: 20120215

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20120501

A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20120529

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20150615

Year of fee payment: 3

R150 Certificate of patent or registration of utility model

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

LAPS Cancellation because of no payment of annual fees