CA2515369C - Listeria attenuated for entry into non-phagocytic cells, vaccines comprising the listeria, and methods of use thereof - Google Patents
Listeria attenuated for entry into non-phagocytic cells, vaccines comprising the listeria, and methods of use thereof Download PDFInfo
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- CA2515369C CA2515369C CA2515369A CA2515369A CA2515369C CA 2515369 C CA2515369 C CA 2515369C CA 2515369 A CA2515369 A CA 2515369A CA 2515369 A CA2515369 A CA 2515369A CA 2515369 C CA2515369 C CA 2515369C
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| US44605103P | 2003-02-06 | 2003-02-06 | |
| US60/446,051 | 2003-02-06 | ||
| US44915303P | 2003-02-21 | 2003-02-21 | |
| US60/449,153 | 2003-02-21 | ||
| US49008903P | 2003-07-24 | 2003-07-24 | |
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| US51191903P | 2003-10-15 | 2003-10-15 | |
| US51171903P | 2003-10-15 | 2003-10-15 | |
| US51186903P | 2003-10-15 | 2003-10-15 | |
| US60/511,869 | 2003-10-15 | ||
| US60/511,919 | 2003-10-15 | ||
| US60/511,719 | 2003-10-15 | ||
| US54151504P | 2004-02-02 | 2004-02-02 | |
| US60/541,515 | 2004-02-02 | ||
| PCT/US2004/003429 WO2004110481A2 (en) | 2003-02-06 | 2004-02-06 | Listeria attenuated for entry into non-phagocytic cells, vaccines comprising the listeria, and methods of use thereof |
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Families Citing this family (63)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7820180B2 (en) * | 2004-09-24 | 2010-10-26 | The Trustees Of The University Of Pennsylvania | Listeria-based and LLO-based vaccines |
| US8791237B2 (en) | 1994-11-08 | 2014-07-29 | The Trustees Of The University Of Pennsylvania | Compositions and methods for treatment of non-hodgkins lymphoma |
| US7794729B2 (en) * | 1994-11-08 | 2010-09-14 | The Trustees Of The University Of Pennsylvania | Methods and compositions for immunotherapy of cancer |
| US8956621B2 (en) | 1994-11-08 | 2015-02-17 | The Trustees Of The University Of Pennsylvania | Compositions and methods for treatment of cervical dysplasia |
| US7662396B2 (en) * | 2001-03-26 | 2010-02-16 | The Trustees Of The University Of Pennsylvania | Compositions and methods for enhancing the immunogenicity of antigens |
| US20070264279A1 (en) * | 1994-11-08 | 2007-11-15 | Claudia Gravekamp | Compositions and methods comprising a MAGE-b antigen |
| US8114414B2 (en) * | 1994-11-08 | 2012-02-14 | The Trustees Of The University Of Pennsylvania | Compositions and methods for treatment of cervical cancer |
| WO2001072329A1 (en) * | 2000-03-29 | 2001-10-04 | The Trustees Of The University Of Pennsylvania | Compositions and methods for enhancing immunogenicity of antigens |
| US6099848A (en) * | 1997-11-18 | 2000-08-08 | The Trustees Of The University Of Pennsylvania | Immunogenic compositions comprising DAL/DAT double-mutant, auxotrophic, attenuated strains of Listeria and their methods of use |
| US9012141B2 (en) | 2000-03-27 | 2015-04-21 | Advaxis, Inc. | Compositions and methods comprising KLK3 of FOLH1 antigen |
| US8771702B2 (en) | 2001-03-26 | 2014-07-08 | The Trustees Of The University Of Pennsylvania | Non-hemolytic LLO fusion proteins and methods of utilizing same |
| US7700344B2 (en) | 2001-03-26 | 2010-04-20 | The Trustees Of The University Of Pennsylvania | Compositions and methods for enhancing the immunogenicity of antigens |
| JP2005527240A (ja) * | 2002-05-29 | 2005-09-15 | ザ・レジェンツ・オブ・ザ・ユニバーシティ・オブ・カリフォルニア | 弱毒リステリア種とこれを使用する方法 |
| PT1592441E (pt) * | 2003-02-06 | 2012-05-21 | Aduro Biotech | Listeria atenuada relativamente à entrada em células não patogénicas, vacinas compreendendo a listeria e métodos para sua utilização |
| US7695725B2 (en) | 2003-02-06 | 2010-04-13 | Aduro Biotech | Modified free-living microbes, vaccine compositions and methods of use thereof |
| MXPA05008340A (es) * | 2003-02-06 | 2006-03-13 | Cerus Corp | Microbios de vida libre modificados, composiciones de vacuna y metodos de uso de los mismos. |
| US20050175630A1 (en) * | 2003-12-23 | 2005-08-11 | Eyal Raz | Immunogenic compositions and methods of use thereof |
| US7842289B2 (en) * | 2003-12-24 | 2010-11-30 | Aduro Biotech | Recombinant nucleic acid molecules, expression cassettes, and bacteria, and methods of use thereof |
| US20060003454A1 (en) * | 2004-07-02 | 2006-01-05 | Conjugon, Inc. | Non-dividing donor cells for gene transfer |
| US20060121053A1 (en) * | 2004-10-18 | 2006-06-08 | Pamela Sweeney | High cell density process for growth of Listeria |
| US20060270040A1 (en) * | 2005-05-26 | 2006-11-30 | Conjugon, Inc. | Compositions and methods for treating tissue |
| US7935804B2 (en) | 2006-03-01 | 2011-05-03 | Aduro Biotech | Engineered Listeria and methods of use thereof |
| US20070207171A1 (en) * | 2006-03-01 | 2007-09-06 | Cerus Corporation | Engineered listeria and methods of use thereof |
| EP2860253B1 (en) | 2006-03-01 | 2018-08-01 | Aduro Biotech, Inc. | Engineered listeria and methods of use thereof |
| US8926993B2 (en) | 2006-07-17 | 2015-01-06 | Aduro Biotech | Methods and compositions using Listeria for enhancing immunogenicity by prime boost |
| JP2009545330A (ja) * | 2006-08-04 | 2009-12-24 | ザ トラスティーズ オブ ザ ユニバーシティー オブ ぺンシルべニア | Ｉｇｅ依存性疾患を治療するための方法および組成物 |
| PT2977456T (pt) * | 2006-08-15 | 2018-02-05 | Univ Pennsylvania | Composições compreendendo hmw-maa e seus fragmentos para tratamento do cancro |
| US8268326B2 (en) * | 2006-08-15 | 2012-09-18 | The Trustees Of The University Of Pennsylvania | Compositions comprising HMW-MAA and fragments thereof, and methods of use thereof |
| WO2008066774A2 (en) | 2006-11-22 | 2008-06-05 | The Regents Of The University Of California | INTERFERON-β PRODUCTION MODULATING LISTERIA STRAINS AND METHODS FOR USING SAME |
| EP2170931A4 (en) * | 2007-06-15 | 2010-06-30 | Immurx Inc | USE OF TLR AGONISTS AND / OR TYPE 1 INTERFERONS TO MITIGATE THE TOXICITY OF THERAPEUTIC REGIMES OF TNF-R AGONISTS |
| US8709445B2 (en) * | 2007-07-31 | 2014-04-29 | Los Angeles Biomedical Research Institute At Harbor-Ucla Medical Center | Vaccination with killed but metabolically active (KBMA) protozoans with toll-like receptor agonists |
| US9650639B2 (en) | 2008-05-19 | 2017-05-16 | Advaxis, Inc. | Dual delivery system for heterologous antigens |
| DK2853269T3 (da) | 2008-05-19 | 2019-08-05 | Advaxis Inc | Dobbelt indgivelsessystem til heterologe antigener, der omfatter en rekombinant Listeria-stamme svækket ved mutation af dal/dat og deletion af ActA, der omfatter et nukleinsyremolekyle, der koder for et listeriolysin O-prostataspecifikt antigenfusionsprotein |
| US9017660B2 (en) | 2009-11-11 | 2015-04-28 | Advaxis, Inc. | Compositions and methods for prevention of escape mutation in the treatment of Her2/neu over-expressing tumors |
| CN102076843A (zh) | 2008-05-19 | 2011-05-25 | 艾杜罗生物科技公司 | 包含prfa*突变体李斯特菌的组合物及其使用方法 |
| PT2403935T (pt) | 2009-03-04 | 2017-09-22 | Univ Pennsylvania | Composições compreendendo fatores angiogénicos e metedos para a sua utilização |
| US10016617B2 (en) | 2009-11-11 | 2018-07-10 | The Trustees Of The University Of Pennsylvania | Combination immuno therapy and radiotherapy for the treatment of Her-2-positive cancers |
| US9161974B2 (en) | 2010-05-23 | 2015-10-20 | Aduro Biotech, Inc. | Methods and compositions using listeria for adjuvant treatment of cancer |
| CN103282048B (zh) | 2010-10-01 | 2017-05-17 | 宾夕法尼亚大学理事会 | 李斯特菌疫苗载体用于在寄生虫感染的个体中扭转免疫无应答的用途 |
| SI2640842T1 (sl) | 2010-11-17 | 2018-09-28 | Aduro Biotech, Inc. | Postopki in sestavki za induciranje imunskega odziva na EGFRvIII |
| CN103687611A (zh) | 2011-03-11 | 2014-03-26 | 阿德瓦希斯公司 | 基于李斯特菌属的佐剂 |
| CA2836360C (en) | 2011-05-23 | 2019-07-02 | Lego A/S | A toy construction system for augmented reality |
| KR20140134695A (ko) | 2012-03-12 | 2014-11-24 | 어드박시스, 인크. | 리스테리아 백신 치료 후 억제 세포 기능 저해 |
| AU2015333632A1 (en) | 2014-10-14 | 2017-06-01 | The Trustees Of The University Of Pennsylvania | Combination therapy for use in cancer therapy |
| US10688166B2 (en) | 2014-11-03 | 2020-06-23 | Cerus Corporation | Compositions and methods for improved car-T cell therapies |
| WO2016168214A2 (en) | 2015-04-13 | 2016-10-20 | Aduro Biotech, Inc. | Immunogenic fusion proteins for the treatment of cancer |
| CR20170507A (es) | 2015-04-13 | 2018-02-13 | Aduro Biotech Inc | Fusiones de variante iii del receptor de factor de crecimiento epidérmico-mesotelina y métodos para usar los mismos. |
| CA2990864A1 (en) | 2015-06-26 | 2016-12-29 | Cerus Corporation | Cryoprecipitate compositions and methods of preparation thereof |
| WO2017070619A1 (en) | 2015-10-23 | 2017-04-27 | Cerus Corporation | Plasma compositions and methods of use thereof |
| RU2729116C2 (ru) | 2015-12-16 | 2020-08-04 | Гритстоун Онколоджи, Инк. | Идентификация, производство и применение неоантигенов |
| EP3589297A1 (en) | 2017-03-03 | 2020-01-08 | Cerus Corporation | Kits and methods for preparing pathogen-inactivated platelet compositions |
| JP7284156B2 (ja) | 2017-09-19 | 2023-05-30 | アドバクシス， インコーポレイテッド | 細菌またはＬｉｓｔｅｒｉａ株の凍結乾燥のための組成物および方法 |
| AU2018348165A1 (en) | 2017-10-10 | 2020-05-21 | Gritstone Bio, Inc. | Neoantigen identification using hotspots |
| US11885815B2 (en) | 2017-11-22 | 2024-01-30 | Gritstone Bio, Inc. | Reducing junction epitope presentation for neoantigens |
| CN111770789B (zh) | 2017-12-29 | 2023-05-05 | 塞鲁斯公司 | 用于处理生物流体的系统和方法 |
| SG11202008820WA (en) | 2018-03-09 | 2020-10-29 | Advaxis Inc | Compositions and methods for evaluating attenuation and infectivity of listeria strains |
| WO2019210034A1 (en) | 2018-04-27 | 2019-10-31 | Advaxis, Inc. | Compositions and methods for evaluating potency of listeria-based immunotherapeutics |
| EP3838915A4 (en) * | 2018-08-02 | 2022-02-09 | Suzhou Royaltech Med Co., Ltd | TUMOR IMMUNOOTHERAPY COMPOSITION BASED ON ANTIGEN-CONTAINING CELLS ACTIVATED BY ATTENTIONED LISTERIA MONOCYTOGENES, METHOD OF MANUFACTURE THEREOF AND APPLICATION THEREOF |
| MX2021015653A (es) | 2019-06-22 | 2022-04-11 | Cerus Corp | Sistemas de tratamiento de fluidos biologicos. |
| EP3991179A1 (en) | 2019-06-28 | 2022-05-04 | Cerus Corporation | System and methods for implementing a biological fluid treatment device |
| US10973908B1 (en) | 2020-05-14 | 2021-04-13 | David Gordon Bermudes | Expression of SARS-CoV-2 spike protein receptor binding domain in attenuated salmonella as a vaccine |
| CN113845589B (zh) * | 2021-08-20 | 2023-04-07 | 扬州大学 | SmcL单克隆抗体、分泌该抗体的杂交瘤细胞株及其应用和竞争ELISA检测方法 |
| CN114250244A (zh) * | 2021-12-14 | 2022-03-29 | 浙江农林大学 | 以重组减毒单増李斯特菌为载体的结肠癌疫苗及制备方法 |
Family Cites Families (82)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4545987A (en) | 1983-12-20 | 1985-10-08 | Advanced Genetics Research Institute | Psoralen inactivated double-stranded RNA viral vaccines |
| US5106619A (en) | 1983-12-20 | 1992-04-21 | Diamond Scientific Co. | Preparation of inactivated viral vaccines |
| US4556556A (en) | 1984-03-23 | 1985-12-03 | Advanced Genetics Research Institute | Vaccine for the prevention of vesicular stomatitis virus infection |
| US5171568A (en) | 1984-04-06 | 1992-12-15 | Chiron Corporation | Recombinant herpes simplex gb-gd vaccine |
| US4791062A (en) | 1985-02-28 | 1988-12-13 | Diamond Scientific Co. | FVR vaccine |
| NZ227011A (en) | 1987-11-18 | 1992-03-26 | Chiron Corp | Non-a, non-b-hepatitis (hepatitis c) antigens, proteins, nucleotide sequences, vaccines and kits |
| DK189788D0 (da) | 1988-04-07 | 1988-04-07 | Wolf Watz Hans | Vaccine |
| HUT54896A (en) | 1989-03-17 | 1991-04-29 | Chiron Corp | Process for producing aquous diagnosticum and vaccine of nanbv |
| EP0398748B1 (en) | 1989-05-18 | 2002-01-09 | Chiron Corporation | NANBV diagnostics: polynucleotides useful for screening for hepatitis C virus |
| US5180819A (en) | 1989-12-22 | 1993-01-19 | The Trustees Of Columbia University In The City Of New York | Purified myeloblastin, nucleic acid molecule encoding same, and uses thereof |
| US5830702A (en) | 1990-10-31 | 1998-11-03 | The Trustees Of The University Of Pennsylvania | Live, recombinant listeria monocytogenes and production of cytotoxic T-cell response |
| FR2686896B1 (fr) * | 1992-01-31 | 1995-01-06 | Pasteur Institut | Mutant attenue de listeria monocytogenes; souche recombinante de listeria monocytogenes, utilisation comme vecteurs heterologues d'antigenes vaccinal et utilisation comme vaccin ou composition diagnostique. |
| US5399719A (en) | 1993-06-28 | 1995-03-21 | Steritech, Inc. | Compounds for the photodecontamination of pathogens in blood |
| US5593823A (en) | 1993-06-28 | 1997-01-14 | Cerus Corporation | Method for inactivating pathogens in blood using photoactivation of 4'-primary amino-substituted psoralens |
| US6051237A (en) | 1994-11-08 | 2000-04-18 | The Trustees Of The University Of Pennsylvania | Specific immunotherapy of cancer using a live recombinant bacterial vaccine vector |
| WO2001072329A1 (en) | 2000-03-29 | 2001-10-04 | The Trustees Of The University Of Pennsylvania | Compositions and methods for enhancing immunogenicity of antigens |
| US5691132A (en) | 1994-11-14 | 1997-11-25 | Cerus Corporation | Method for inactivating pathogens in red cell compositions using quinacrine mustard |
| US6682729B1 (en) | 1995-05-03 | 2004-01-27 | University Of Maryland, Baltimore | Method for introducing and expressing genes in animal cells, and live invasive bacterial vectors for use in the same |
| US5877159A (en) | 1995-05-03 | 1999-03-02 | University Of Maryland At Baltimore | Method for introducing and expressing genes in animal cells and live invasive bacterial vectors for use in the same |
| US6150170A (en) | 1998-05-03 | 2000-11-21 | University Of Maryland At Baltimore | Method for introducing and expressing genes in animal cells, and live invasive bacterial vectors for use in the same |
| US6177441B1 (en) | 1995-06-05 | 2001-01-23 | Cerus Corporation | Treating red blood cell solutions with anti-viral agents |
| CA2199731A1 (en) | 1995-06-07 | 1996-12-19 | Susan Wollowitz | Treating red blood cell solutions with anti-viral agents |
| GB9521568D0 (en) | 1995-10-20 | 1995-12-20 | Lynxvale Ltd | Delivery of biologically active polypeptides |
| WO1997022349A1 (en) | 1995-12-20 | 1997-06-26 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for in vivo t cell activation by antigen-pulsed dendritic cells |
| US6083502A (en) | 1996-01-05 | 2000-07-04 | The United States Of America As Represented By The Department Of Health And Human Services | Mesothelium antigen and methods and kits for targeting it |
| US5843459A (en) | 1996-01-19 | 1998-12-01 | Human Gene Therapy Research Institute | Differential inactivation of nucleic acids by chemical modification |
| WO1997034472A1 (en) * | 1996-03-22 | 1997-09-25 | Yale University | Methods for inducing immune responsiveness in a subject |
| BR9708387A (pt) | 1996-03-28 | 2000-01-04 | Genitrix Llc | Processo para vacinação de um mamìfero a um antìgeno selecionado, células patogênica e hospedeira, composição de vacina, ácido nucleico, e, opsonina engenheirada. |
| EP0808897A1 (en) | 1996-05-21 | 1997-11-26 | I.D.M. Immuno-Designed Molecules | New antigen presenting cells, a process for preparing the same and their use as cellular vaccines |
| CA2259140C (en) | 1996-07-10 | 2008-09-30 | Immunex Corporation | Method of activating dendritic cells |
| GB9615022D0 (en) | 1996-07-17 | 1996-09-04 | Mini Agriculture & Fisheries | Vaccine preparations |
| US7273753B2 (en) | 1996-08-02 | 2007-09-25 | Center Of Blood Research | Purification and uses of dendritic cells and monocytes |
| US6194204B1 (en) | 1996-08-02 | 2001-02-27 | Center For Blood Research, Inc. | Enrichment of dendritic cells from blood |
| DE19635676A1 (de) | 1996-09-03 | 1998-03-05 | Basf Ag | Feste geschäumte Wirkstoffzubereitungen |
| TW555562B (en) * | 1996-12-27 | 2003-10-01 | Kirin Brewery | Method for activation of human antigen-presenting cells, activated human antigen-presenting cells and use thereof |
| US6514987B1 (en) | 1997-01-06 | 2003-02-04 | Cerus Corporation | Frangible compounds for pathogen inactivation |
| WO1998030545A1 (en) | 1997-01-06 | 1998-07-16 | Cerus Corporation | Frangible compounds for pathogen inactivation |
| US6093725A (en) | 1997-01-06 | 2000-07-25 | Cerus Corporation | Frangible compounds for pathogen inactivation |
| GB9700939D0 (en) | 1997-01-17 | 1997-03-05 | Microbial Technics Limited | Therapy |
| WO1998033386A1 (en) | 1997-01-31 | 1998-08-06 | Vanderbilt University | Method of delivering antigens for vaccination with a live vector |
| AU748074B2 (en) | 1997-07-21 | 2002-05-30 | Cerus Corporation | Method of treating leukocytes, leukocyte compositions and methods of use thereof |
| EP0902086A1 (en) * | 1997-08-22 | 1999-03-17 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Tuberculosis vaccine |
| US6099848A (en) | 1997-11-18 | 2000-08-08 | The Trustees Of The University Of Pennsylvania | Immunogenic compositions comprising DAL/DAT double-mutant, auxotrophic, attenuated strains of Listeria and their methods of use |
| EP1032265B1 (en) | 1997-11-20 | 2003-10-29 | Cerus Corporation | New psoralens for pathogen inactivation |
| DE19754938B4 (de) * | 1997-12-11 | 2006-04-20 | Christoph von Dr. Eichel-Streiber | TGC-Verfahren zur Induktion einer zielgerichteten, somatischen Transgenität |
| US6143551A (en) | 1997-12-29 | 2000-11-07 | Schering Aktiengesellschaft | Delivery of polypeptide-encoding plasmid DNA into the cytosol of macrophages by attenuated listeria suicide bacteria |
| DE69826521T2 (de) | 1998-01-06 | 2005-09-29 | Cerus Corp., Concord | Verfahren zum quenchen von pathogen-inaktivatoren in biologischen materialien |
| AU756911B2 (en) | 1998-03-20 | 2003-01-23 | Biopolo S.C.R.L. | Processes for improved presentation of antigens by dendritic cells |
| AU3456099A (en) | 1998-03-31 | 1999-10-18 | Geron Corporation | Methods and compositions for eliciting an immune response to a telomerase antigen |
| US20020155108A1 (en) | 1998-05-04 | 2002-10-24 | Biocrystal, Ltd. | Method for ex vivo loading of antigen presenting cells with antigen, and a vaccine comprising the loaded cells |
| US6254863B1 (en) | 1998-08-12 | 2001-07-03 | Loma Linda University | Non-virulent Porphyromonas gingivalis mutant |
| US6004815A (en) | 1998-08-13 | 1999-12-21 | The Regents Of The University Of California | Bacteria expressing nonsecreted cytolysin as intracellular microbial delivery vehicles to eukaryotic cells |
| GB9918283D0 (en) | 1999-08-03 | 1999-10-06 | Int Centre Genetic Eng & Bio | Hsv vaccines |
| WO2001024637A1 (en) | 1999-10-04 | 2001-04-12 | Vion Pharmaceuticals, Inc. | Methods for treating solid tumors with irradiation and bacteria |
| DE19949594A1 (de) * | 1999-10-14 | 2001-04-26 | Deutsches Krebsforsch | Rekombinante attenuierte Listerien zur Immuntherapie |
| US20030077263A1 (en) | 1999-10-29 | 2003-04-24 | Immunex Corporation | Method of activating dendritic cells |
| GB0007150D0 (en) * | 2000-03-24 | 2000-05-17 | Lamellar Therapeutics Limited | Immunotherapeutic methods and compositions |
| US6855320B2 (en) | 2000-03-29 | 2005-02-15 | The Trustees Of The University Of Pennsylvania | Fusion of non-hemolytic, truncated form of listeriolysin O to antigens to enhance immunogenicity |
| CZ20023662A3 (cs) | 2000-05-04 | 2003-04-16 | President And Fellows Of Harvard College | Sloučeniny a způsoby léčení a prevence bakteriální infekce |
| EP1320602A2 (en) | 2000-09-21 | 2003-06-25 | The Regents Of The University Of California | Spas-1 cancer antigen |
| ATE287945T1 (de) | 2000-11-14 | 2005-02-15 | Univ Bruxelles | Herstellung und verwendung von dendritischen zellen |
| WO2003061379A2 (en) | 2000-12-21 | 2003-07-31 | Cerus Corporation | Methods for inactivation of pathogens in biological materials |
| US6783765B2 (en) | 2001-03-23 | 2004-08-31 | Council Of Scientific And Industrial Research | Process for the preparation of a vaccine for the treatment of tuberculosis and other intracellular infections diseases and the vaccine produced by the process |
| EP1373475A2 (en) | 2001-04-02 | 2004-01-02 | Alimentary Health Limited | Immunotherapy based on dendritic cells |
| US7566568B2 (en) | 2001-04-27 | 2009-07-28 | Istituto Superiore Di Sanita | Method for generating highly active human dendritic cells from peripheral blood mononuclear cells |
| US20040022761A1 (en) | 2001-05-11 | 2004-02-05 | Banchereau Jacques F | Compositions and methods for producing antigen-presenting cells |
| WO2003012085A1 (en) | 2001-07-30 | 2003-02-13 | Sigma-Tau Industrie Farmaceutiche Riunite S.P.A. | Antigen presenting cells, method for their preparation and their use for cancer vaccines |
| US7413869B2 (en) | 2002-04-05 | 2008-08-19 | Dendreon Corporation | Method for determining potency of antigenic presenting cell based vaccines |
| US7425449B2 (en) | 2002-04-30 | 2008-09-16 | The Regents Of The University Of California | Site specific Listeria integration vectors and methods for using the same |
| JP2005527240A (ja) | 2002-05-29 | 2005-09-15 | ザ・レジェンツ・オブ・ザ・ユニバーシティ・オブ・カリフォルニア | 弱毒リステリア種とこれを使用する方法 |
| US20040009194A1 (en) | 2002-06-21 | 2004-01-15 | Jean-Marie Andrieu | Methods, and compositions for a therapeutic antigen presenting cell vaccine for treatment of immunodeficiency virus |
| AU2002950473A0 (en) | 2002-07-26 | 2002-09-12 | Commonwealth Scientific And Industrial Research Organisation | Expression system |
| MXPA05008340A (es) | 2003-02-06 | 2006-03-13 | Cerus Corp | Microbios de vida libre modificados, composiciones de vacuna y metodos de uso de los mismos. |
| PT1592441E (pt) * | 2003-02-06 | 2012-05-21 | Aduro Biotech | Listeria atenuada relativamente à entrada em células não patogénicas, vacinas compreendendo a listeria e métodos para sua utilização |
| US7695725B2 (en) | 2003-02-06 | 2010-04-13 | Aduro Biotech | Modified free-living microbes, vaccine compositions and methods of use thereof |
| AU2004281834A1 (en) | 2003-10-15 | 2005-04-28 | Cerus Corporation | Listeria-based EphA2 vaccines |
| US20050175630A1 (en) | 2003-12-23 | 2005-08-11 | Eyal Raz | Immunogenic compositions and methods of use thereof |
| US7842289B2 (en) | 2003-12-24 | 2010-11-30 | Aduro Biotech | Recombinant nucleic acid molecules, expression cassettes, and bacteria, and methods of use thereof |
| MXPA06007144A (es) | 2003-12-24 | 2007-01-31 | Cerus Corp | Moleculas de acido nucleico recombinante que codifican proteinas de fusion que comprenden polipeptidos de senal secretoria bacterial y de antigenos, casetes de expresion y bacterias, y metodos para usar las mismas. |
| WO2005067460A2 (en) | 2003-12-24 | 2005-07-28 | Medimmune, Inc. | Epha2 vaccines |
| US20070031457A1 (en) | 2004-02-06 | 2007-02-08 | Dubensky Thomas W Jr | Modified Bacillus anthracis, vaccine compositions and methods of use thereof |
| WO2005092372A2 (en) | 2004-02-06 | 2005-10-06 | Cerus Corporation | Modified bacillus anthracis vaccine compositions and methods of use thereof |
-
2004
- 2004-02-06 PT PT04749316T patent/PT1592441E/pt unknown
- 2004-02-06 EP EP04749316A patent/EP1592441B1/en not_active Expired - Lifetime
- 2004-02-06 KR KR1020057014538A patent/KR101192652B1/ko active IP Right Grant
- 2004-02-06 ES ES04749316T patent/ES2382332T3/es not_active Expired - Lifetime
- 2004-02-06 US US10/773,792 patent/US7691393B2/en active Active
- 2004-02-06 JP JP2006532281A patent/JP4545151B2/ja not_active Expired - Fee Related
- 2004-02-06 DK DK04749316.8T patent/DK1592441T3/da active
- 2004-02-06 CA CA2515369A patent/CA2515369C/en not_active Expired - Fee Related
- 2004-02-06 AT AT04749316T patent/ATE552843T1/de active
- 2004-02-06 MX MXPA05008342A patent/MXPA05008342A/es active IP Right Grant
- 2004-02-06 AU AU2004246999A patent/AU2004246999B2/en not_active Ceased
- 2004-02-06 WO PCT/US2004/003429 patent/WO2004110481A2/en active Application Filing
-
2006
- 2006-02-23 HK HK06102459.4A patent/HK1082187A1/xx not_active IP Right Cessation
-
2007
- 2007-02-05 JP JP2007026201A patent/JP2007125040A/ja active Pending
-
2010
- 2010-03-29 US US12/748,586 patent/US8287883B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| US8287883B2 (en) | 2012-10-16 |
| EP1592441B1 (en) | 2012-04-11 |
| ES2382332T3 (es) | 2012-06-07 |
| JP4545151B2 (ja) | 2010-09-15 |
| JP2007125040A (ja) | 2007-05-24 |
| EP1592441A2 (en) | 2005-11-09 |
| CA2515369A1 (en) | 2004-12-23 |
| KR20050105460A (ko) | 2005-11-04 |
| WO2004110481A3 (en) | 2005-03-03 |
| US7691393B2 (en) | 2010-04-06 |
| HK1082187A1 (en) | 2006-06-02 |
| MXPA05008342A (es) | 2006-02-28 |
| AU2004246999A1 (en) | 2004-12-23 |
| AU2004246999B2 (en) | 2010-06-10 |
| WO2004110481A2 (en) | 2004-12-23 |
| JP2007503846A (ja) | 2007-03-01 |
| ATE552843T1 (de) | 2012-04-15 |
| PT1592441E (pt) | 2012-05-21 |
| US20110201092A1 (en) | 2011-08-18 |
| DK1592441T3 (da) | 2012-05-14 |
| US20040228877A1 (en) | 2004-11-18 |
| KR101192652B1 (ko) | 2012-10-19 |
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