CA2469980A1 - Methods and devices for removal of organic molecules from biological mixtures using anion exchange - Google Patents
Methods and devices for removal of organic molecules from biological mixtures using anion exchange Download PDFInfo
- Publication number
- CA2469980A1 CA2469980A1 CA002469980A CA2469980A CA2469980A1 CA 2469980 A1 CA2469980 A1 CA 2469980A1 CA 002469980 A CA002469980 A CA 002469980A CA 2469980 A CA2469980 A CA 2469980A CA 2469980 A1 CA2469980 A1 CA 2469980A1
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- CA
- Canada
- Prior art keywords
- negatively charged
- biological sample
- sample mixture
- anion exchange
- exchange material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
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- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
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- B01L2400/0677—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
- B01L2400/0683—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers mechanically breaking a wall or membrane within a channel or chamber
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y10T436/255—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.] including use of a solid sorbent, semipermeable membrane, or liquid extraction
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Landscapes
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
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- Crystallography & Structural Chemistry (AREA)
- Dispersion Chemistry (AREA)
- Hematology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Treatment Of Water By Ion Exchange (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10/027,222 | 2001-12-20 | ||
| US10/027,222 US7192560B2 (en) | 2001-12-20 | 2001-12-20 | Methods and devices for removal of organic molecules from biological mixtures using anion exchange |
| PCT/US2002/037973 WO2003054509A2 (en) | 2001-12-20 | 2002-11-27 | Methods and devices for removal of organic molecules from biological mixtures using anion exchange |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2469980A1 true CA2469980A1 (en) | 2003-07-03 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002469980A Abandoned CA2469980A1 (en) | 2001-12-20 | 2002-11-27 | Methods and devices for removal of organic molecules from biological mixtures using anion exchange |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US7192560B2 (https=) |
| EP (2) | EP2116305B1 (https=) |
| JP (1) | JP2005514014A (https=) |
| AT (1) | ATE437697T1 (https=) |
| AU (1) | AU2002366944A1 (https=) |
| CA (1) | CA2469980A1 (https=) |
| DE (1) | DE60233162D1 (https=) |
| WO (1) | WO2003054509A2 (https=) |
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| US7347976B2 (en) * | 2001-12-20 | 2008-03-25 | 3M Innovative Properties Company | Methods and devices for removal of organic molecules from biological mixtures using a hydrophilic solid support in a hydrophobic matrix |
| US7192560B2 (en) * | 2001-12-20 | 2007-03-20 | 3M Innovative Properties Company | Methods and devices for removal of organic molecules from biological mixtures using anion exchange |
| US6889468B2 (en) * | 2001-12-28 | 2005-05-10 | 3M Innovative Properties Company | Modular systems and methods for using sample processing devices |
| US20050271553A1 (en) * | 2002-01-04 | 2005-12-08 | Applera Corporation | Petal-array support for use with microplates |
| EP1525053A1 (en) * | 2002-07-26 | 2005-04-27 | Applera Corporation | Device and method for purification of nucleic acids |
| US6750261B1 (en) * | 2003-04-08 | 2004-06-15 | 3M Innovative Properties Company | High internal phase emulsion foams containing polyelectrolytes |
| US7981600B2 (en) * | 2003-04-17 | 2011-07-19 | 3M Innovative Properties Company | Methods and devices for removal of organic molecules from biological mixtures using an anion exchange material that includes a polyoxyalkylene |
| US7238269B2 (en) * | 2003-07-01 | 2007-07-03 | 3M Innovative Properties Company | Sample processing device with unvented channel |
| US7322254B2 (en) * | 2003-12-12 | 2008-01-29 | 3M Innovative Properties Company | Variable valve apparatus and methods |
| US20050130177A1 (en) * | 2003-12-12 | 2005-06-16 | 3M Innovative Properties Company | Variable valve apparatus and methods |
| US7837947B2 (en) * | 2003-12-12 | 2010-11-23 | 3M Innovative Properties Company | Sample mixing on a microfluidic device |
| US7727710B2 (en) | 2003-12-24 | 2010-06-01 | 3M Innovative Properties Company | Materials, methods, and kits for reducing nonspecific binding of molecules to a surface |
| US20050142570A1 (en) * | 2003-12-24 | 2005-06-30 | 3M Innovative Properties Company | Methods for nucleic acid isolation and kits using a microfluidic device and sedimenting reagent |
| US7939249B2 (en) * | 2003-12-24 | 2011-05-10 | 3M Innovative Properties Company | Methods for nucleic acid isolation and kits using a microfluidic device and concentration step |
| US20050142571A1 (en) * | 2003-12-24 | 2005-06-30 | 3M Innovative Properties Company | Methods for nucleic acid isolation and kits using solid phase material |
| US20060160122A1 (en) * | 2004-02-18 | 2006-07-20 | Applera Corporation | Polyelectrolyte-coated size-exclusion ion-exchange particles |
| US20050196856A1 (en) * | 2004-02-18 | 2005-09-08 | Applera Corporation | Polyelectrolyte-coated size-exclusion ion-exchange particles |
| US20050181378A1 (en) * | 2004-02-18 | 2005-08-18 | Applera Corporation | Polyelectrolyte-coated size-exclusion ion-exchange particles |
| JP2005257337A (ja) * | 2004-03-09 | 2005-09-22 | Brother Ind Ltd | 検査対象受体、検査装置、及び検査方法 |
| EP1650297B1 (en) * | 2004-10-19 | 2011-04-13 | Samsung Electronics Co., Ltd. | Method and apparatus for the rapid disruption of cells or viruses using micro magnetic beads and laser |
| KR100601972B1 (ko) * | 2004-11-03 | 2006-07-18 | 삼성전자주식회사 | 레이저와 비드를 이용한 상 분리에 의한 핵산의 정제 장치및 방법 |
| KR100601974B1 (ko) * | 2004-11-25 | 2006-07-18 | 삼성전자주식회사 | 비드의 상이한 레이저 흡수에 의한 핵산의 정제 장치 및방법 |
| KR100647320B1 (ko) * | 2005-02-05 | 2006-11-23 | 삼성전자주식회사 | 혈청 분리용 마이크로 장치 |
| US7763210B2 (en) * | 2005-07-05 | 2010-07-27 | 3M Innovative Properties Company | Compliant microfluidic sample processing disks |
| US7323660B2 (en) | 2005-07-05 | 2008-01-29 | 3M Innovative Properties Company | Modular sample processing apparatus kits and modules |
| USD564667S1 (en) | 2005-07-05 | 2008-03-18 | 3M Innovative Properties Company | Rotatable sample processing disk |
| US7754474B2 (en) * | 2005-07-05 | 2010-07-13 | 3M Innovative Properties Company | Sample processing device compression systems and methods |
| CN101147065A (zh) * | 2005-07-25 | 2008-03-19 | 爱科米株式会社 | 免疫测定方法及该方法中使用的免疫测定用试剂盒 |
| US8173413B2 (en) | 2005-08-11 | 2012-05-08 | University Of Washington | Separation and concentration of biological cells and biological particles using a one-dimensional channel |
| US7993821B2 (en) * | 2005-08-11 | 2011-08-09 | University Of Washington | Methods and apparatus for the isolation and enrichment of circulating tumor cells |
| EP1963016B1 (en) * | 2005-11-14 | 2017-04-26 | Rijk Zwaan Zaadteelt en Zaadhandel B.V. | Method for removing a fluid from a container and device therefor |
| KR101157174B1 (ko) * | 2005-11-24 | 2012-06-20 | 삼성전자주식회사 | 세포 또는 바이러스의 신속한 파괴 방법 및 장치 |
| US8656949B2 (en) | 2006-08-15 | 2014-02-25 | University Of Maryland College Park | Microfluidic devices and methods of fabrication |
| EP1894617B1 (en) | 2006-08-31 | 2013-08-14 | Samsung Electronics Co., Ltd. | Method of mixing at least two kinds of fluids in centrifugal micro-fluid treating substrate |
| JP4984849B2 (ja) * | 2006-11-27 | 2012-07-25 | パナソニック株式会社 | 成分分離デバイスと、この成分分離デバイスを用いた化学分析デバイス |
| WO2008068019A1 (en) * | 2006-12-06 | 2008-06-12 | Ashe Morris Ltd | Improved flow reactor |
| AU2007336774A1 (en) | 2006-12-22 | 2008-07-03 | 3M Innovative Properties Company | Thermal transfer methods and structures for microfluidic systems |
| KR20090105934A (ko) * | 2006-12-22 | 2009-10-07 | 쓰리엠 이노베이티브 프로퍼티즈 컴파니 | 향상된 샘플 처리 장치, 시스템 및 방법 |
| US20090042241A1 (en) * | 2007-04-06 | 2009-02-12 | California Institute Of Technology | Microfluidic device |
| KR20100017126A (ko) * | 2007-04-25 | 2010-02-16 | 쓰리엠 이노베이티브 프로퍼티즈 컴파니 | 화학적 요소 및 처리 장치 조립체 |
| US20100062421A1 (en) * | 2007-04-25 | 2010-03-11 | Wensheng Xia | Compositions, methods, and devices for isolating biological materials |
| ES2453379T3 (es) * | 2007-04-25 | 2014-04-07 | 3M Innovative Properties Company | Reactivos soportados, métodos y dispositivos |
| US20100129878A1 (en) * | 2007-04-25 | 2010-05-27 | Parthasarathy Ranjani V | Methods for nucleic acid amplification |
| US8841135B2 (en) | 2007-06-20 | 2014-09-23 | University Of Washington | Biochip for high-throughput screening of circulating tumor cells |
| CN102007222A (zh) * | 2007-08-13 | 2011-04-06 | 3M创新有限公司 | 用于检测耐药微生物的方法 |
| CA2700532A1 (en) * | 2007-09-28 | 2009-04-02 | 3M Innovative Properties Company | Dual oligonucleotide method of nucleic acid detection |
| US20100209927A1 (en) * | 2007-11-06 | 2010-08-19 | Menon Vinod P | Processing device tablet |
| DE102007063102B4 (de) * | 2007-12-28 | 2022-02-10 | Robert Bosch Gmbh | Verfahren zur Erfassung eines periodisch pulsierenden Betriebsparameters |
| WO2009086343A2 (en) * | 2007-12-31 | 2009-07-09 | 3M Innovative Properties Company | Microorganism-capturing compositions and methods |
| CN101615512B (zh) * | 2008-06-24 | 2013-02-06 | 通用电气公司 | 超级电容装置及其制造方法 |
| EP2248915B1 (en) * | 2009-05-05 | 2013-09-18 | Qiagen GmbH | Detection of multiple nucleic acid sequences in a reaction cartridge |
| EP2281630A1 (en) * | 2009-08-07 | 2011-02-09 | Labor Diagnostika Nord GmbH & Co. KG | Sample clean-up device |
| US9445795B2 (en) * | 2009-10-16 | 2016-09-20 | Confluent Surgical, Inc. | Prevention of premature gelling of delivery devices for pH dependent forming materials |
| USD638951S1 (en) | 2009-11-13 | 2011-05-31 | 3M Innovative Properties Company | Sample processing disk cover |
| US8834792B2 (en) | 2009-11-13 | 2014-09-16 | 3M Innovative Properties Company | Systems for processing sample processing devices |
| USD667561S1 (en) | 2009-11-13 | 2012-09-18 | 3M Innovative Properties Company | Sample processing disk cover |
| USD638550S1 (en) | 2009-11-13 | 2011-05-24 | 3M Innovative Properties Company | Sample processing disk cover |
| US20110117607A1 (en) * | 2009-11-13 | 2011-05-19 | 3M Innovative Properties Company | Annular compression systems and methods for sample processing devices |
| JP2011196846A (ja) * | 2010-03-19 | 2011-10-06 | Soka Univ | 検出容器およびそれを使用する微粒子状試料を検出する方法 |
| GB201005742D0 (en) | 2010-04-06 | 2010-05-19 | Ashe Morris Ltd | Improved tubular reactor |
| US20120156767A1 (en) * | 2010-12-17 | 2012-06-21 | Stmicroelectronics S.R.L. | Pcr device |
| US9168523B2 (en) | 2011-05-18 | 2015-10-27 | 3M Innovative Properties Company | Systems and methods for detecting the presence of a selected volume of material in a sample processing device |
| AU2012255144B2 (en) | 2011-05-18 | 2015-01-29 | Diasorin Italia S.P.A. | Systems and methods for volumetric metering on a sample processing device |
| CN103501908B (zh) | 2011-05-18 | 2016-03-16 | 3M创新有限公司 | 用于样品处理装置上阀调的系统和方法 |
| USD672467S1 (en) | 2011-05-18 | 2012-12-11 | 3M Innovative Properties Company | Rotatable sample processing disk |
| JP2013117424A (ja) * | 2011-12-02 | 2013-06-13 | Enplas Corp | 流体取扱装置 |
| EP2811983B1 (en) | 2012-09-17 | 2019-05-01 | W.R. Grace & CO. - CONN. | Functionalized particulate support material and methods of making and using the same |
| EP2812091B1 (en) | 2012-09-17 | 2021-03-10 | W.R. Grace & CO. - CONN. | Chromatography media and devices |
| DE102012220250A1 (de) * | 2012-11-07 | 2014-05-08 | Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. | Fluidikmodul für eine zentrifugale filtration und verfahren zum filtern einer probe |
| US20150307927A1 (en) * | 2012-12-15 | 2015-10-29 | Tangen Biosciences, Inc. | Apparatus for centrifuge mountable manifold for processing fluidic assays |
| US10610861B2 (en) | 2012-12-17 | 2020-04-07 | Accellix Ltd. | Systems, compositions and methods for detecting a biological condition |
| US20140170678A1 (en) | 2012-12-17 | 2014-06-19 | Leukodx Ltd. | Kits, compositions and methods for detecting a biological condition |
| JP6349327B2 (ja) | 2012-12-17 | 2018-06-27 | レウコドゥックス，リミテッド | 化学的状態を判定するためのシステムおよび方法 |
| KR101497193B1 (ko) * | 2012-12-31 | 2015-03-03 | 고려대학교 산학협력단 | 원심력 미세유동 기반 혈소판 복합기능 및 약물반응 검사 장치 및 방법 |
| DE102013203655B4 (de) * | 2013-03-04 | 2023-03-23 | Robert Bosch Gmbh | Verfahren und Vorrichtung zur selektiven Lyse von zellulären Partikeln |
| CN103352061A (zh) * | 2013-07-23 | 2013-10-16 | 江南大学 | 一种提高发酵法生产硫酸软骨素类似物产量的方法 |
| ES2887110T3 (es) | 2014-01-16 | 2021-12-21 | Grace W R & Co | Medios para cromatografía de afinidad y dispositivos para cromatografía |
| PL3137209T3 (pl) | 2014-05-02 | 2023-01-02 | W.R. Grace & Co. - Conn. | Funkcjonalizowany materiał nośnikowy i sposoby wytwarzania oraz stosowania funkcjonalizowanego materiału nośnikowego |
| EP3009845A1 (en) * | 2014-10-17 | 2016-04-20 | Laboratorios Alpha San Ignacio Pharma S.L. | Portable analyzer for automatically performing immunoassays, and for analyzing and interpreting the results thereof |
| EP3215260B1 (en) | 2014-11-03 | 2020-01-15 | Tangen Biosciences Inc. | Apparatus and method for cell, spore, or virus capture and disruption |
| KR102566292B1 (ko) | 2015-06-05 | 2023-08-10 | 더블유.알. 그레이스 앤드 캄파니-콘. | 흡착성 바이오프로세싱 정화제와 이의 제조 및 사용 방법 |
| US10797567B2 (en) | 2015-07-23 | 2020-10-06 | Life Technologies Corporation | Rotor assembly including a housing for a sensor array component and methods for using same |
| CN107850581B (zh) * | 2015-12-29 | 2021-01-05 | 株式会社爱思迪科学 | 含有浓度显著不同的多种成分的试样的分析预处理方法 |
| US10625263B2 (en) | 2016-03-28 | 2020-04-21 | Tangen Biosciences, Inc. | Apparatus and method for extracting pathogens from biological samples |
| TWI741658B (zh) | 2018-01-24 | 2021-10-01 | 美商伊路米納有限公司 | 流體緩衝 |
| US20200064254A1 (en) * | 2018-08-23 | 2020-02-27 | Truvian Sciences, Inc. | Devices With Optically Readable Liquid Reservoirs |
| CN111558402B (zh) * | 2020-03-10 | 2021-05-11 | 青岛英赛特生物科技有限公司 | 一种气压驱动的离心式微流控检测芯片 |
| USD1116151S1 (en) | 2023-05-03 | 2026-03-03 | Truvian Sciences, Inc. | Monolayer |
Family Cites Families (110)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3157635A (en) | 1960-08-05 | 1964-11-17 | Takeda Chemical Industries Ltd | Sulfonic acid cation exchange resin purification of nucleotides |
| US4399009A (en) | 1981-01-19 | 1983-08-16 | Oronzio Denora Impianti Elettrochimici S.P.A. | Electrolytic cell and method |
| US4399235A (en) * | 1981-05-11 | 1983-08-16 | The Dow Chemical Company | High density ion exchange resins |
| US4780367A (en) | 1983-06-27 | 1988-10-25 | Minnesota Mining And Manufacturing Company | Tackified star block copolymer pressure-sensitive adhesive composition and the sheet materials coated therewith |
| US4849311A (en) | 1986-09-24 | 1989-07-18 | Toa Nenryo Kogyo Kabushiki Kaisha | Immobilized electrolyte membrane |
| US4753640A (en) | 1986-10-06 | 1988-06-28 | Catheter Technology Corporation | Catheters and methods |
| US4935342A (en) * | 1986-12-01 | 1990-06-19 | Syngene, Inc. | Method of isolating and purifying nucleic acids from biological samples |
| US4923978A (en) | 1987-12-28 | 1990-05-08 | E. I. Du Pont De Nemours & Company | Process for purifying nucleic acids |
| JPH01171970A (ja) | 1987-12-28 | 1989-07-06 | Tokyo Electric Co Ltd | プリンタ |
| JPH0645099Y2 (ja) * | 1988-02-24 | 1994-11-16 | エヌオーケー株式会社 | 密封装置 |
| EP0409432A3 (en) | 1989-07-20 | 1991-12-11 | Warner-Lambert Company | Confectionery delivery system |
| JP2978187B2 (ja) | 1989-11-02 | 1999-11-15 | 日本ケミカルリサーチ株式会社 | 修飾スーパーオキサイドディスムターゼの製造法 |
| US5187066A (en) | 1990-02-14 | 1993-02-16 | Syntex (U.S.A.) Inc. | Methods for detecting amphiphilic antigens |
| US5770029A (en) | 1996-07-30 | 1998-06-23 | Soane Biosciences | Integrated electrophoretic microdevices |
| EP0447362A1 (en) | 1990-03-13 | 1991-09-18 | Warner-Lambert Company | Improved ingestible anion exchange resin delivery system compositions containing adipic acid |
| US5334316A (en) | 1990-10-10 | 1994-08-02 | Brigham Young University | Process of using polytetraalkylammonium and polytrialkylamine-containing ligands bonded to inorganic supports for removing and concentrating desired ions from solutions |
| US5294668A (en) | 1990-11-15 | 1994-03-15 | Minnesota Mining And Manufacturing Company | Polyolefin pressure-sensitive adhesive compositions containing macromonomers |
| US5264184A (en) | 1991-03-19 | 1993-11-23 | Minnesota Mining And Manufacturing Company | Device and a method for separating liquid samples |
| JPH0830704B2 (ja) | 1991-03-21 | 1996-03-27 | イーストマン コダック カンパニー | 核酸増幅のための要素および方法ならびに付着プローブを使用する検出方法 |
| US6093558A (en) | 1991-07-25 | 2000-07-25 | Edge Biosystems, Inc. | Binding protein of biologically active compositions to an adhesive formulation on a substrate |
| JPH0550263A (ja) | 1991-08-20 | 1993-03-02 | Mitsubishi Electric Corp | フラツシユ溶接機の電源装置 |
| JPH0550263U (ja) * | 1991-12-13 | 1993-07-02 | エヌオーケー株式会社 | 弁機構 |
| US5380901A (en) | 1992-01-30 | 1995-01-10 | The United States Of America As Represented By The Secretary Of Commerce | Multifunctional acrylates and the synthesis thereof |
| US5183705A (en) | 1992-02-28 | 1993-02-02 | Minnesota Mining And Manufacturing Company | Pressure-sensitive adhesive composition having high shear strength |
| DE69316231T2 (de) | 1992-10-21 | 1998-08-20 | Cornell Res Foundation Inc | Selektive, die porengrösse betreffende chemische modifikation poröser materialien |
| US6780818B2 (en) * | 1994-02-02 | 2004-08-24 | The Regents Of The University Of California | Quantitative organic vapor-particle sampler |
| US5670346A (en) | 1994-03-08 | 1997-09-23 | Amersham International Plc | Modifying nucleotide analogues |
| JP3546463B2 (ja) | 1994-03-31 | 2004-07-28 | 三菱化学株式会社 | 混合イオン交換樹脂床の分離用球状不活性樹脂 |
| US5639428A (en) | 1994-07-19 | 1997-06-17 | Becton Dickinson And Company | Method and apparatus for fully automated nucleic acid amplification, nucleic acid assay and immunoassay |
| SE9500183D0 (sv) | 1995-01-20 | 1995-01-20 | Pharmacia Biotech Ab | Method for the purifivation of short nucleic acids |
| US6063838A (en) | 1995-02-16 | 2000-05-16 | 3M Innovative Properties Company | Blended pressure-sensitive adhesives |
| US5741828A (en) | 1995-05-01 | 1998-04-21 | S.K.Y. Polymers, Inc. | Flexible hydrophilic composite coatings |
| US6143247A (en) * | 1996-12-20 | 2000-11-07 | Gamera Bioscience Inc. | Affinity binding-based system for detecting particulates in a fluid |
| US5856174A (en) * | 1995-06-29 | 1999-01-05 | Affymetrix, Inc. | Integrated nucleic acid diagnostic device |
| US6168948B1 (en) * | 1995-06-29 | 2001-01-02 | Affymetrix, Inc. | Miniaturized genetic analysis systems and methods |
| US5700902A (en) | 1995-07-27 | 1997-12-23 | Circe Biomedical, Inc. | Block copolymers |
| AU702403B2 (en) * | 1995-12-05 | 1999-02-18 | Gamera Bioscience | Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system with on-board informatics |
| US20010055812A1 (en) * | 1995-12-05 | 2001-12-27 | Alec Mian | Devices and method for using centripetal acceleration to drive fluid movement in a microfluidics system with on-board informatics |
| JP3818689B2 (ja) * | 1996-01-16 | 2006-09-06 | 富士写真フイルム株式会社 | コロイド状シリカをコアとし、有機ポリマーをシェルとするコア／シェル状複合粒子の水性分散物及びその製造方法 |
| DK0840804T3 (da) | 1996-01-23 | 2000-07-17 | Rapigene Inc | Fremgangsmåder og sammensætninger til analyse af nukleinsyremolekyler ved anvendelse af størrelsesseparationsteknikker |
| FR2744803B1 (fr) * | 1996-02-12 | 1998-03-13 | Bio Merieux | Procede et dispositif de traitement d'une carte d'analyse |
| US5863801A (en) * | 1996-06-14 | 1999-01-26 | Sarnoff Corporation | Automated nucleic acid isolation |
| US6074827A (en) | 1996-07-30 | 2000-06-13 | Aclara Biosciences, Inc. | Microfluidic method for nucleic acid purification and processing |
| AU4775497A (en) | 1996-09-19 | 1998-04-14 | W. Kurt Roth | Method for purifying and eventually analyzing nucleic acids from biological test samples |
| DE19731670C2 (de) | 1997-07-23 | 2000-06-29 | Dorothea Waschk | Verfahren zur Reinigung und gegebenenfalls Analyse von Nukleinsäuren aus biologischen Proben |
| AU4807197A (en) | 1996-10-03 | 1998-04-24 | Paul Bunn | Hydrophilic microparticles and methods to prepare same |
| JP4451931B2 (ja) | 1996-11-12 | 2010-04-14 | ホアットマン，インコーポレーテッド | 親水性のポリマー状の相転換メンブレン |
| US5997818A (en) | 1997-02-27 | 1999-12-07 | Minnesota Mining And Manufacturing Company | Cassette for tonometric calibration |
| SE9700769D0 (sv) | 1997-03-04 | 1997-03-04 | Pharmacia Biotech Ab | Matriser för separation och separation som utnyttjar matriserna |
| GB9709728D0 (en) * | 1997-05-13 | 1997-07-02 | Dynal As | Single step method |
| US6632399B1 (en) | 1998-05-22 | 2003-10-14 | Tecan Trading Ag | Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system for performing biological fluid assays |
| US6451260B1 (en) * | 1997-08-26 | 2002-09-17 | Dyax Corp. | Method for producing microporous elements, the microporous elements thus produced and uses thereof |
| AU744879B2 (en) | 1997-09-19 | 2002-03-07 | Aclara Biosciences, Inc. | Apparatus and method for transferring liquids |
| JP2001517794A (ja) | 1997-09-19 | 2001-10-09 | アクレイラ バイオサイエンシズ，インコーポレイティド | キャピラリ電気フロー装置および方法 |
| AU2347799A (en) | 1998-01-29 | 1999-08-16 | University Of Pittsburgh | Thermal expansion-induced fluid control for microfluidic devices |
| WO1999046591A2 (en) | 1998-03-10 | 1999-09-16 | Strategic Diagnostics, Inc. | Integrated assay device and methods of production and use |
| US6265168B1 (en) * | 1998-10-06 | 2001-07-24 | Transgenomic, Inc. | Apparatus and method for separating and purifying polynucleotides |
| WO1999058664A1 (en) | 1998-05-14 | 1999-11-18 | Whitehead Institute For Biomedical Research | Solid phase technique for selectively isolating nucleic acids |
| US6103199A (en) | 1998-09-15 | 2000-08-15 | Aclara Biosciences, Inc. | Capillary electroflow apparatus and method |
| US6277488B1 (en) | 1998-10-28 | 2001-08-21 | 3M Innovative Properties Company | Adhesive composition containing a block copolymer composition and polyphenylene oxide resin and products thereof |
| US6240790B1 (en) * | 1998-11-09 | 2001-06-05 | Agilent Technologies, Inc. | Device for high throughout sample processing, analysis and collection, and methods of use thereof |
| DE19858443A1 (de) * | 1998-12-17 | 2000-07-06 | Inst Mikrotechnik Mainz Gmbh | Verfahren zum Abgeben eines Fluids, fluidisches Bauteil sowie Vorrichtung zur Handhabung solcher Bauteile |
| AU4424299A (en) | 1999-02-01 | 2000-08-18 | 3M Innovative Properties Company | Poly(alpha-olefin) adhesive cover tapes for analytical receptacles |
| WO2000050887A1 (de) * | 1999-02-22 | 2000-08-31 | Evotec Biosystems Ag | Verwendung von trägermaterial in der kapillar-elektrochromatographie |
| US6306273B1 (en) | 1999-04-13 | 2001-10-23 | Aclara Biosciences, Inc. | Methods and compositions for conducting processes in microfluidic devices |
| JP4094232B2 (ja) * | 1999-04-16 | 2008-06-04 | 三菱レイヨン株式会社 | 新規なアミダーゼ遺伝子 |
| EP1177267A1 (en) | 1999-05-05 | 2002-02-06 | 3M Innovative Properties Company | Silicone adhesives, articles, and methods |
| GB9915398D0 (en) | 1999-07-02 | 1999-09-01 | Baker Matthew J | Magnetic particles |
| US6524456B1 (en) | 1999-08-12 | 2003-02-25 | Ut-Battelle, Llc | Microfluidic devices for the controlled manipulation of small volumes |
| US6383783B1 (en) | 1999-09-21 | 2002-05-07 | 3M Innovative Properties Company | Nucleic acid isolation by adhering to hydrophobic solid phase and removing with nonionic surfactant |
| US6414136B1 (en) | 1999-10-06 | 2002-07-02 | Prolinx, Inc. | Removal of dye-labeled dideoxy terminators from DNA sequencing reactions |
| US6521438B1 (en) * | 1999-11-05 | 2003-02-18 | North Carolina State University | Chemoreceptors in plant parasitic nematodes |
| GB9927904D0 (en) | 1999-11-25 | 2000-01-26 | Amersham Pharm Biotech Ab | A method fro obtaining a nucleic acid variant |
| CA2290731A1 (en) | 1999-11-26 | 2001-05-26 | D. Jed Harrison | Apparatus and method for trapping bead based reagents within microfluidic analysis system |
| US6875348B2 (en) * | 2000-02-18 | 2005-04-05 | The Board Of Trustees Of The Leland Stanford Junior University | Separation column having a photopolymerized sol-gel component and associated methods |
| AU2001238616A1 (en) | 2000-02-23 | 2001-09-03 | Wen Shao | Rapid nucleic acid separation, isolation and purification methods |
| US6454924B2 (en) * | 2000-02-23 | 2002-09-24 | Zyomyx, Inc. | Microfluidic devices and methods |
| WO2001068913A2 (en) | 2000-03-13 | 2001-09-20 | Genset | Nucleic acid detection method and system |
| WO2001068240A2 (en) | 2000-03-14 | 2001-09-20 | Hammen Corporation | Composite matrices with interstitial polymer networks |
| AU6380001A (en) | 2000-03-24 | 2001-10-03 | Qiagen Gmbh | Porous ferro- or ferrimagnetic glass particles for isolating molecules |
| US6734401B2 (en) * | 2000-06-28 | 2004-05-11 | 3M Innovative Properties Company | Enhanced sample processing devices, systems and methods |
| US6720187B2 (en) * | 2000-06-28 | 2004-04-13 | 3M Innovative Properties Company | Multi-format sample processing devices |
| US6627159B1 (en) * | 2000-06-28 | 2003-09-30 | 3M Innovative Properties Company | Centrifugal filling of sample processing devices |
| US6504021B2 (en) | 2000-07-05 | 2003-01-07 | Edge Biosystems, Inc. | Ion exchange method for DNA purification |
| AU2002241515A1 (en) * | 2000-11-06 | 2002-06-18 | The University Of Houston System | Nucleic acid separation using immobilized metal affinity chromatography |
| US20030017567A1 (en) | 2001-04-24 | 2003-01-23 | 3M Innovative Properties Company | Biological sample processing methods and compositions that include surfactants |
| US6617136B2 (en) * | 2001-04-24 | 2003-09-09 | 3M Innovative Properties Company | Biological sample processing methods and compositions that include surfactants |
| US6825032B2 (en) * | 2001-05-14 | 2004-11-30 | Sigma-Aldrich Co. | High capacity assay platforms |
| US7374724B2 (en) * | 2001-05-29 | 2008-05-20 | Tecan Trading Ag | Device for processing samples, use of the device, and method for producing the device |
| US6682702B2 (en) * | 2001-08-24 | 2004-01-27 | Agilent Technologies, Inc. | Apparatus and method for simultaneously conducting multiple chemical reactions |
| US7189368B2 (en) * | 2001-09-17 | 2007-03-13 | Gyros Patent Ab | Functional unit enabling controlled flow in a microfluidic device |
| US7347976B2 (en) * | 2001-12-20 | 2008-03-25 | 3M Innovative Properties Company | Methods and devices for removal of organic molecules from biological mixtures using a hydrophilic solid support in a hydrophobic matrix |
| US7192560B2 (en) * | 2001-12-20 | 2007-03-20 | 3M Innovative Properties Company | Methods and devices for removal of organic molecules from biological mixtures using anion exchange |
| US6833238B2 (en) | 2002-01-04 | 2004-12-21 | Applera Corporation | Petal-array support for use with microplates |
| US20030228709A1 (en) * | 2002-03-25 | 2003-12-11 | Kozlowski Roland Zbignieiw | Arrays |
| US20040023220A1 (en) | 2002-07-23 | 2004-02-05 | Lawrence Greenfield | Integrated method for PCR cleanup and oligonucleotide removal |
| US7214348B2 (en) | 2002-07-26 | 2007-05-08 | Applera Corporation | Microfluidic size-exclusion devices, systems, and methods |
| EP1552013A1 (en) | 2002-07-26 | 2005-07-13 | Applera Corporation | Microfluidic device including purification column with excess diluent, and method |
| CA2492613A1 (en) | 2002-07-26 | 2004-02-05 | Applera Corporation | Microfluidic size-exclusion devices, systems, and methods |
| US20040018559A1 (en) | 2002-07-26 | 2004-01-29 | Applera Corporation | Size-exclusion ion-exchange particles |
| EP1525053A1 (en) | 2002-07-26 | 2005-04-27 | Applera Corporation | Device and method for purification of nucleic acids |
| US7981600B2 (en) * | 2003-04-17 | 2011-07-19 | 3M Innovative Properties Company | Methods and devices for removal of organic molecules from biological mixtures using an anion exchange material that includes a polyoxyalkylene |
| US20050130177A1 (en) * | 2003-12-12 | 2005-06-16 | 3M Innovative Properties Company | Variable valve apparatus and methods |
| US7322254B2 (en) * | 2003-12-12 | 2008-01-29 | 3M Innovative Properties Company | Variable valve apparatus and methods |
| US20050142570A1 (en) * | 2003-12-24 | 2005-06-30 | 3M Innovative Properties Company | Methods for nucleic acid isolation and kits using a microfluidic device and sedimenting reagent |
| US7939249B2 (en) * | 2003-12-24 | 2011-05-10 | 3M Innovative Properties Company | Methods for nucleic acid isolation and kits using a microfluidic device and concentration step |
| US20050142571A1 (en) * | 2003-12-24 | 2005-06-30 | 3M Innovative Properties Company | Methods for nucleic acid isolation and kits using solid phase material |
| US7727710B2 (en) * | 2003-12-24 | 2010-06-01 | 3M Innovative Properties Company | Materials, methods, and kits for reducing nonspecific binding of molecules to a surface |
| JP4422623B2 (ja) | 2005-01-17 | 2010-02-24 | 株式会社日立ハイテクノロジーズ | 化学分析装置および化学分析カートリッジ |
| KR100682947B1 (ko) * | 2005-06-04 | 2007-02-15 | 삼성전자주식회사 | 고정화된 금속-리간드 복합체에 의한 핵산 정제 방법 및장치 |
-
2001
- 2001-12-20 US US10/027,222 patent/US7192560B2/en not_active Expired - Fee Related
-
2002
- 2002-11-27 AU AU2002366944A patent/AU2002366944A1/en not_active Abandoned
- 2002-11-27 JP JP2003555173A patent/JP2005514014A/ja active Pending
- 2002-11-27 AT AT02791325T patent/ATE437697T1/de not_active IP Right Cessation
- 2002-11-27 CA CA002469980A patent/CA2469980A1/en not_active Abandoned
- 2002-11-27 WO PCT/US2002/037973 patent/WO2003054509A2/en not_active Ceased
- 2002-11-27 DE DE60233162T patent/DE60233162D1/de not_active Expired - Lifetime
- 2002-11-27 EP EP09163155.6A patent/EP2116305B1/en not_active Expired - Lifetime
- 2002-11-27 EP EP02791325A patent/EP1461155B1/en not_active Expired - Lifetime
-
2005
- 2005-09-14 US US11/226,161 patent/US7871827B2/en not_active Expired - Lifetime
-
2007
- 2007-02-22 US US11/709,373 patent/US20070160504A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| WO2003054509A2 (en) | 2003-07-03 |
| DE60233162D1 (de) | 2009-09-10 |
| US20030138779A1 (en) | 2003-07-24 |
| US20070160504A1 (en) | 2007-07-12 |
| WO2003054509A3 (en) | 2004-03-18 |
| ATE437697T1 (de) | 2009-08-15 |
| US20060013732A1 (en) | 2006-01-19 |
| JP2005514014A (ja) | 2005-05-19 |
| US7192560B2 (en) | 2007-03-20 |
| EP1461155B1 (en) | 2009-07-29 |
| EP1461155A4 (en) | 2006-10-04 |
| EP2116305B1 (en) | 2013-05-01 |
| EP1461155A2 (en) | 2004-09-29 |
| AU2002366944A1 (en) | 2003-07-09 |
| US7871827B2 (en) | 2011-01-18 |
| AU2002366944A8 (en) | 2003-07-09 |
| EP2116305A1 (en) | 2009-11-11 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Discontinued |
Effective date: 20121127 |