CA2382741A1 - Methodes de production d'une molecule cible chez un animal transgenique et de purification de la molecule cible - Google Patents
Methodes de production d'une molecule cible chez un animal transgenique et de purification de la molecule cible Download PDFInfo
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- CA2382741A1 CA2382741A1 CA002382741A CA2382741A CA2382741A1 CA 2382741 A1 CA2382741 A1 CA 2382741A1 CA 002382741 A CA002382741 A CA 002382741A CA 2382741 A CA2382741 A CA 2382741A CA 2382741 A1 CA2382741 A1 CA 2382741A1
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- 101001023030 Toxoplasma gondii Myosin-D Proteins 0.000 description 1
- 101710150448 Transcriptional regulator Myc Proteins 0.000 description 1
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- 102000052547 Wnt-1 Human genes 0.000 description 1
- 108700020987 Wnt-1 Proteins 0.000 description 1
- 230000009471 action Effects 0.000 description 1
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- 235000020244 animal milk Nutrition 0.000 description 1
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- 238000001574 biopsy Methods 0.000 description 1
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- 239000011616 biotin Substances 0.000 description 1
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- 210000000988 bone and bone Anatomy 0.000 description 1
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- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
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- 238000000423 cell based assay Methods 0.000 description 1
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- 230000009920 chelation Effects 0.000 description 1
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- 230000004927 fusion Effects 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 1
- 229960003180 glutathione Drugs 0.000 description 1
- 102000035122 glycosylated proteins Human genes 0.000 description 1
- 108091005608 glycosylated proteins Proteins 0.000 description 1
- 235000020251 goat milk Nutrition 0.000 description 1
- 239000000122 growth hormone Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
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- 230000005802 health problem Effects 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
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- 238000001727 in vivo Methods 0.000 description 1
- 238000005462 in vivo assay Methods 0.000 description 1
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- 238000010348 incorporation Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
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- 210000004072 lung Anatomy 0.000 description 1
- 229960000274 lysozyme Drugs 0.000 description 1
- 235000010335 lysozyme Nutrition 0.000 description 1
- 239000004325 lysozyme Substances 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 230000023247 mammary gland development Effects 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
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- 229910052759 nickel Inorganic materials 0.000 description 1
- PXHVJJICTQNCMI-UHFFFAOYSA-N nickel Substances [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 125000003835 nucleoside group Chemical group 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 238000002823 phage display Methods 0.000 description 1
- 239000008177 pharmaceutical agent Substances 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 210000001236 prokaryotic cell Anatomy 0.000 description 1
- 230000012743 protein tagging Effects 0.000 description 1
- 230000002797 proteolythic effect Effects 0.000 description 1
- 230000006337 proteolytic cleavage Effects 0.000 description 1
- 108010043277 recombinant soluble CD4 Proteins 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 235000014347 soups Nutrition 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 210000004243 sweat Anatomy 0.000 description 1
- 238000001308 synthesis method Methods 0.000 description 1
- 210000001550 testis Anatomy 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 238000011824 transgenic rat model Methods 0.000 description 1
- 230000010474 transient expression Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- 239000013638 trimer Substances 0.000 description 1
- 239000013603 viral vector Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 210000002268 wool Anatomy 0.000 description 1
- 235000021246 κ-casein Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2207/00—Modified animals
- A01K2207/15—Humanized animals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/01—Animal expressing industrially exogenous proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Animal Husbandry (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
L'invention concerne des systèmes et des méthodes de production et de purification de molécules cibles présentes dans des systèmes biologiques. Les systèmes et les méthodes de l'invention utilisent l'expression transgénique de polypeptides de fixation multivalents, en tant que supports d'affinité, pour purifier lesdites molécules cibles. Les polypeptides de fixation multivalents transgéniques fixent à la fois les molécules cibles, par exemple, un épitope fixable d'une molécule cible, et une matrice.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US15974899P | 1999-10-14 | 1999-10-14 | |
| US60/159,748 | 1999-10-14 | ||
| US20466200P | 2000-05-17 | 2000-05-17 | |
| US60/204,662 | 2000-05-17 | ||
| PCT/US2000/028589 WO2001026455A1 (fr) | 1999-10-14 | 2000-10-16 | Methodes de production d'une molecule cible chez un animal transgenique et de purification de la molecule cible |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2382741A1 true CA2382741A1 (fr) | 2001-04-19 |
Family
ID=26856252
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002382741A Abandoned CA2382741A1 (fr) | 1999-10-14 | 2000-10-16 | Methodes de production d'une molecule cible chez un animal transgenique et de purification de la molecule cible |
Country Status (14)
| Country | Link |
|---|---|
| EP (1) | EP1220606A4 (fr) |
| JP (1) | JP2003511048A (fr) |
| KR (1) | KR100772465B1 (fr) |
| CN (1) | CN1322003C (fr) |
| AU (1) | AU781944B2 (fr) |
| BR (1) | BR0014860A (fr) |
| CA (1) | CA2382741A1 (fr) |
| HU (1) | HUP0203537A2 (fr) |
| IL (1) | IL149003A0 (fr) |
| MX (1) | MXPA02003702A (fr) |
| NO (1) | NO20021686L (fr) |
| NZ (1) | NZ518263A (fr) |
| RU (1) | RU2002112460A (fr) |
| WO (1) | WO2001026455A1 (fr) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030049694A1 (en) * | 2001-09-10 | 2003-03-13 | Chung-Hsiun Wu | Production of fusion proteins and use for identifying binding molecules |
| US6800462B2 (en) * | 2001-09-10 | 2004-10-05 | Abgenomics Corporation | Production of recombinant proteins in vivo and use for generating antibodies |
| CN1283785C (zh) * | 2002-05-20 | 2006-11-08 | 上海杰隆生物工程股份有限公司 | 利用转基因动物乳腺生产人促红细胞生成素的方法 |
| WO2007052096A2 (fr) * | 2005-06-29 | 2007-05-10 | Cameron Malcolm Lang Clokie | Production de proteines morphogeniques osseuses dans des mammiferes transgeniques |
| JP2010528677A (ja) * | 2007-06-13 | 2010-08-26 | スターンベルド バイオテクノロジー ノース アメリカ， インコーポレイテッド | 乳中に外因性タンパク質を産生するトランスジェニック哺乳動物 |
| US9157097B2 (en) | 2008-09-25 | 2015-10-13 | Proteovec Holding, L.L.C. | Vectors for production of growth hormone |
| US9150880B2 (en) | 2008-09-25 | 2015-10-06 | Proteovec Holding, L.L.C. | Vectors for production of antibodies |
| WO2010118360A1 (fr) | 2009-04-09 | 2010-10-14 | The Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Production de protéines au moyen de vecteurs à base de transposon |
| WO2013096458A1 (fr) * | 2011-12-19 | 2013-06-27 | Gtc Biotherapeutics, Inc. | Alpha-1-antitrypsine humaine recombinante pour le traitement de troubles inflammatoires |
| EP2956003A2 (fr) | 2013-02-13 | 2015-12-23 | Laboratoire Français du Fractionnement et des Biotechnologies | Protéines à glycosylation modifiée et leurs procédés de production |
| JP2016508515A (ja) | 2013-02-13 | 2016-03-22 | ラボラトワール フランセ デュ フラクショヌマン エ デ ビオテクノロジーＬａｂｏｒａｔｏｉｒｅ Ｆｒａｎｃａｉｓ ｄｕ Ｆｒａｃｔｉｏｎｎｅｍｅｎｔ ｅｔ ｄｅｓ Ｂｉｏｔｅｃｈｎｏｌｏｇｉｅｓ | 高ガラクトシル化抗ＴＮＦ−α抗体およびその使用 |
| JP2016532100A (ja) | 2013-07-05 | 2016-10-13 | ラボラトワール・フランセ・デュ・フラクシオンマン・エ・デ・ビョテクノロジーＬａｂｏｒａｔｏｉｒｅ Ｆｒａｎｃａｉｓ Ｄｕ Ｆｒａｃｔｉｏｎｎｅｍｅｎｔ Ｅｔ Ｄｅｓ Ｂｉｏｔｅｃｈｎｏｌｏｇｉｅｓ | アフィニティークロマトグラフィーマトリックス |
| FR3025515B1 (fr) | 2014-09-05 | 2016-09-09 | Lab Francais Du Fractionnement | Procede de purification d'un anticorps monoclonal |
| FR3034420A1 (fr) | 2015-03-31 | 2016-10-07 | Lab Francais Du Fractionnement | Anticorps monoclonaux anti-cd303 |
| JP6712125B2 (ja) * | 2015-09-04 | 2020-06-17 | 株式会社フジクラ | 酸化物超電導線材の接続構造体およびその製造方法 |
| FR3060394B1 (fr) | 2016-12-16 | 2019-05-24 | Laboratoire Francais Du Fractionnement Et Des Biotechnologies | Combinaison d'anticorps anti-cd303 et anti-amhrii |
| FR3060395B1 (fr) | 2016-12-16 | 2019-05-24 | Laboratoire Francais Du Fractionnement Et Des Biotechnologies | Combinaison d'anticorps anti-cd303 et anti-her2 |
| FR3076294B1 (fr) | 2017-12-29 | 2022-01-28 | Lab Francais Du Fractionnement | Procede de purification d'anticorps a partir de lait brut |
| CA3223956A1 (fr) | 2021-12-20 | 2023-06-29 | Vetoquinol Sa | Anticorps anti-recepteur a de l'interleukine-31 canin (il-31ra) et leurs utilisations |
| WO2024165823A2 (fr) | 2023-02-09 | 2024-08-15 | Commissariat A L'energie Atomique Et Aux Energies Alternatives | Fragment fab mutant pour l'obtention de conjugués mono- ou bi-fonctionnalisés site-spécifiques |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK580789D0 (da) * | 1989-11-20 | 1989-11-20 | Novo Nordisk As | Fremgangsmaade til oprensning af polypeptider |
| US5639940A (en) * | 1994-03-03 | 1997-06-17 | Pharmaceutical Proteins Ltd. | Production of fibrinogen in transgenic animals |
| CN1114357A (zh) * | 1994-07-01 | 1996-01-03 | 唐勇炜 | 利用转基因动物生产重组多肽 |
-
2000
- 2000-10-16 NZ NZ518263A patent/NZ518263A/en unknown
- 2000-10-16 BR BR0014860-1A patent/BR0014860A/pt not_active Application Discontinuation
- 2000-10-16 IL IL14900300A patent/IL149003A0/xx unknown
- 2000-10-16 WO PCT/US2000/028589 patent/WO2001026455A1/fr active IP Right Grant
- 2000-10-16 EP EP00970945A patent/EP1220606A4/fr not_active Withdrawn
- 2000-10-16 CA CA002382741A patent/CA2382741A1/fr not_active Abandoned
- 2000-10-16 RU RU2002112460/13A patent/RU2002112460A/ru unknown
- 2000-10-16 KR KR1020027004804A patent/KR100772465B1/ko not_active IP Right Cessation
- 2000-10-16 JP JP2001529255A patent/JP2003511048A/ja active Pending
- 2000-10-16 AU AU80254/00A patent/AU781944B2/en not_active Ceased
- 2000-10-16 HU HU0203537A patent/HUP0203537A2/hu unknown
- 2000-10-16 CN CNB008152802A patent/CN1322003C/zh not_active Expired - Fee Related
- 2000-10-16 MX MXPA02003702A patent/MXPA02003702A/es not_active Application Discontinuation
-
2002
- 2002-04-10 NO NO20021686A patent/NO20021686L/no not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| JP2003511048A (ja) | 2003-03-25 |
| AU8025400A (en) | 2001-04-23 |
| IL149003A0 (en) | 2002-11-10 |
| AU781944B2 (en) | 2005-06-23 |
| EP1220606A4 (fr) | 2004-04-14 |
| KR100772465B1 (ko) | 2007-11-02 |
| RU2002112460A (ru) | 2004-02-27 |
| CN1387399A (zh) | 2002-12-25 |
| HUP0203537A2 (hu) | 2003-02-28 |
| EP1220606A1 (fr) | 2002-07-10 |
| KR20030022759A (ko) | 2003-03-17 |
| BR0014860A (pt) | 2003-07-08 |
| NO20021686D0 (no) | 2002-04-10 |
| CN1322003C (zh) | 2007-06-20 |
| WO2001026455A1 (fr) | 2001-04-19 |
| NO20021686L (no) | 2002-06-12 |
| MXPA02003702A (es) | 2002-09-18 |
| NZ518263A (en) | 2004-03-26 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Discontinued |