CA2382063A1 - Terminateurs d'acides nucleiques a charge modifiee - Google Patents

Terminateurs d'acides nucleiques a charge modifiee Download PDF

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Publication number
CA2382063A1
CA2382063A1 CA002382063A CA2382063A CA2382063A1 CA 2382063 A1 CA2382063 A1 CA 2382063A1 CA 002382063 A CA002382063 A CA 002382063A CA 2382063 A CA2382063 A CA 2382063A CA 2382063 A1 CA2382063 A1 CA 2382063A1
Authority
CA
Canada
Prior art keywords
compound
charge
nucleic acid
atoms
moiety
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002382063A
Other languages
English (en)
Inventor
Shiv Kumar
Parke Flick
John Nelson
Patrick Finn
Satyam Nampalli
Matthew Bull
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Global Life Sciences Solutions USA LLC
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2382063A1 publication Critical patent/CA2382063A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Abstract

L'invention concerne des terminateurs d'acide nucléique à charge modifiée présentant la structure (I), dans laquelle Z est mono-, di- ou triphosphate ou thiophosphate, ou boranophosphate correspondant, X est O, CH¿2?, S ou NH, S est un sucre ou un analogue de sucre, B est une base naturelle ou synthétique, L est alkyle, alcényle ou alkynyle éventuellement substitué par un groupe fonctionnel rapporteur, et L, B, S, X ou Z sont substitués par un groupe fonctionnel qui confère une charge négative nette ou une charge positive nette à la structure (I) dans des conditions physiologiques ou lors d'un séquençage d'acides nucléiques. L'invention concerne également une méthode de séquençage d'acides nucléiques faisant intervenir les terminateurs à charge modifiée susmentionnés, ainsi qu'une méthode d'inhibition d'un virus consistant à faire venir une cellule infectée par un virus au contact d'une dose inhibitrice de virus du terminateur à charge modifiée susmentionné.
CA002382063A 1999-09-17 2000-09-16 Terminateurs d'acides nucleiques a charge modifiee Abandoned CA2382063A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US15473999P 1999-09-17 1999-09-17
US60/154,739 1999-09-17
PCT/US2000/025433 WO2001019841A1 (fr) 1999-09-17 2000-09-16 Terminateurs d'acides nucleiques a charge modifiee

Publications (1)

Publication Number Publication Date
CA2382063A1 true CA2382063A1 (fr) 2001-03-22

Family

ID=22552575

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002382063A Abandoned CA2382063A1 (fr) 1999-09-17 2000-09-16 Terminateurs d'acides nucleiques a charge modifiee

Country Status (4)

Country Link
EP (1) EP1214332A1 (fr)
AU (1) AU7494100A (fr)
CA (1) CA2382063A1 (fr)
WO (1) WO2001019841A1 (fr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6811979B2 (en) * 2000-10-11 2004-11-02 Applera Corporation Fluorescent nucleobase conjugates having anionic linkers
US7256019B2 (en) 2001-08-29 2007-08-14 Ge Healthcare Bio-Sciences Corp. Terminal phosphate blocked nucleoside polyphosphates
US7385038B2 (en) 2002-07-18 2008-06-10 Protein Express Co., Ltd. Non-natural labeled amino acid and method of constructing amino acid/tRNA complex
CN101258220A (zh) 2004-09-16 2008-09-03 阿普里拉股份有限公司 荧光染料化合物，共轭物及其应用
EP2312317A3 (fr) 2005-05-03 2011-06-08 Life Technologies Corporation Système de détection fluorescent et jeu de colorants pour une utilisation avec ledit système
US20090246762A1 (en) 2008-03-31 2009-10-01 Applera Corporation, Applied Biosystems Group Nucleic acid terminators incorporating a cationic moiety and methods for their use
JP4621926B2 (ja) 2008-07-24 2011-02-02 国立大学法人九州大学 酵素基質修飾ヌクレオシド三リン酸、核酸プローブ、マルチラベル化核酸プローブ、マルチラベル化核酸プローブの製造方法および標的核酸の検出方法
WO2013056841A1 (fr) 2011-10-19 2013-04-25 Inra (Institut National De La Recherche Agronomique) Procédé de diagnostic de l'encéphalopathie spongiforme transmissible
JP5509440B2 (ja) * 2012-06-22 2014-06-04 国立大学法人九州大学 タンパク質−核酸複合体の製造方法および標的物の検出方法
TW202322824A (zh) 2020-02-18 2023-06-16 美商基利科學股份有限公司 抗病毒化合物
CN117120444A (zh) 2021-04-16 2023-11-24 吉利德科学公司 使用酰胺制备卡巴核苷的方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1340806C (fr) * 1986-07-02 1999-11-02 James Merrill Prober Methode, systeme et reactifs pour le sequencage de l'adn
US5187085A (en) * 1990-09-28 1993-02-16 Applied Biosystems, Inc. Nucleic acid sequence analysis with nucleoside-5'-o-(1-thiotriphosphates)
JPH10509429A (ja) * 1994-10-14 1998-09-14 ストラティジーン ポリヌクレオチドのポルフィリン標識
US5843658A (en) * 1995-03-10 1998-12-01 Hamamatsu Photonics K.K. Method of measuring oligonucleotide decomposing activity
US5986086A (en) * 1997-06-20 1999-11-16 Amersham Pharmacia Biotech Inc. Non-sulfonated cyanine dyes for labeling nucleosides and nucleotides
WO1999037810A1 (fr) * 1998-01-23 1999-07-29 Amersham Pharmacia Biotech, Inc. Technique, solution reactive et kits pour le sequençage de l'adn
ATE299190T1 (de) * 1998-02-04 2005-07-15 Amersham Biosciences Corp Didesoxy farbterminatoren
GB2341189B (en) * 1998-08-31 2001-06-13 Amersham Pharm Biotech Inc Energy transfer dyes

Also Published As

Publication number Publication date
WO2001019841A1 (fr) 2001-03-22
AU7494100A (en) 2001-04-17
EP1214332A1 (fr) 2002-06-19

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued