CA2370638A1 - Procede de transformation de plante - Google Patents

Procede de transformation de plante Download PDF

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Publication number
CA2370638A1
CA2370638A1 CA002370638A CA2370638A CA2370638A1 CA 2370638 A1 CA2370638 A1 CA 2370638A1 CA 002370638 A CA002370638 A CA 002370638A CA 2370638 A CA2370638 A CA 2370638A CA 2370638 A1 CA2370638 A1 CA 2370638A1
Authority
CA
Canada
Prior art keywords
plant
gene
agrobacterium
time
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002370638A
Other languages
English (en)
Inventor
Igor Kardailsky
Maria J. Harrison
Stephen H. Burleigh
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roberts Samuels Noble Foundation Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2370638A1 publication Critical patent/CA2370638A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Ce procédé de transformation génétique de plantes, induit par Agrobacterium au moment de la floraison, peut s'appliquer à des plantes dicotylédones et monocotylédones pouvant être transformées par Agrobacterium, et il consiste à utiliser une infiltration sous vide pour introduire l'ADN-T de Agrobacterium portant un gène d'intérêt dans les plantes, de préférence des plantes que l'on a fait pousser à partir d'une graine vernalisée. Lorsque les graines recueillies à partir des plantes infiltrées arrivent à maturité, on les fait germer et on choisit dans la descendance de ces plantes celles qui portent le transgène. Ce procédé de transformation permet d'obtenir des plantes démontrant une hérédité stable du transgène, sans qu'il soit nécessaire de pratiquer des procédés de régénération, tels que l'embryogenèse ou l'organogenèse somatique.
CA002370638A 1999-04-21 2000-04-20 Procede de transformation de plante Abandoned CA2370638A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13053199P 1999-04-21 1999-04-21
US60/130,531 1999-04-21
PCT/US2000/010704 WO2000063400A2 (fr) 1999-04-21 2000-04-20 Procede de transformation de plante

Publications (1)

Publication Number Publication Date
CA2370638A1 true CA2370638A1 (fr) 2000-10-26

Family

ID=22445129

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002370638A Abandoned CA2370638A1 (fr) 1999-04-21 2000-04-20 Procede de transformation de plante

Country Status (5)

Country Link
EP (1) EP1171618A2 (fr)
AU (1) AU4365200A (fr)
CA (1) CA2370638A1 (fr)
NZ (1) NZ513993A (fr)
WO (1) WO2000063400A2 (fr)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20050003368A (ko) * 2002-04-15 2005-01-10 화이토칼쳐 가부시키가이샤 식물 형질 전환용 시스템
ES2299285B2 (es) * 2004-11-26 2009-12-07 Universidad De Vigo Procedimiento para transformar material vegetal procedente de arboles adultos.
KR20180026562A (ko) 2009-09-22 2018-03-12 메디카고 인코포레이티드 식물-유래 단백질의 제조방법
CN105671108A (zh) 2010-06-02 2016-06-15 沃维公司 甜菊糖苷的重组生产
AU2011325827B2 (en) 2010-11-04 2016-08-04 Medicago Inc. Plant expression system
TWI620816B (zh) 2011-03-23 2018-04-11 苜蓿股份有限公司 植物衍生蛋白回收方法
EP3009508B1 (fr) 2011-08-08 2020-11-25 Evolva SA Production recombinante de glycosides de stéviol
CN105051195B (zh) 2013-02-06 2019-09-06 埃沃尔瓦公司 用于提高莱鲍迪苷d和莱鲍迪苷m之产生的方法
CA2900882A1 (fr) 2013-02-11 2014-08-14 Evolva Sa Production efficace de glycosides steviol dans les hotes recombinants
ES2912291T3 (es) 2014-03-27 2022-05-25 Medicago Inc Elementos potenciadores de CPMV modificados
ES2787042T3 (es) 2014-07-11 2020-10-14 Medicago Inc Modificación de la producción de proteínas en plantas
MX2017001859A (es) 2014-08-11 2017-04-11 Evolva Sa Produccion de glicosidos de esteviol en hospederos recombinantes.
AU2015314251A1 (en) 2014-09-09 2017-03-16 Evolva Sa Production of steviol glycosides in recombinant hosts
SG11201705606PA (en) 2015-01-30 2017-08-30 Evolva Sa Production of steviol glycosides in recombinant hosts
CA2979931A1 (fr) 2015-03-16 2016-09-22 Dsm Ip Assets B.V. Udp-glycosyltransferases
CN107920487A (zh) 2015-07-02 2018-04-17 莫迪卡戈公司 茉莉酸通路激活剂
AU2016307066A1 (en) 2015-08-07 2018-02-08 Evolva Sa Production of steviol glycosides in recombinant hosts
AU2017251462B2 (en) 2016-04-13 2022-02-03 Evolva Sa Production of steviol glycosides in recombinant hosts
EP3458599A1 (fr) 2016-05-16 2019-03-27 Evolva SA Production de glycosides de stéviol dans des hôtes de recombinaison
WO2018083338A1 (fr) 2016-11-07 2018-05-11 Evolva Sa Production de glycosides de stéviol dans des hôtes recombinants
EP4351320A1 (fr) 2021-06-09 2024-04-17 ImmunityBio, Inc. Procédés et systèmes de production d'une protéine d'intérêt dans une plante

Also Published As

Publication number Publication date
AU4365200A (en) 2000-11-02
WO2000063400A2 (fr) 2000-10-26
NZ513993A (en) 2001-09-28
EP1171618A2 (fr) 2002-01-16
WO2000063400A3 (fr) 2001-01-18

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