CA2352488A1 - Procede de transformation de vegetaux - Google Patents

Procede de transformation de vegetaux Download PDF

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Publication number
CA2352488A1
CA2352488A1 CA002352488A CA2352488A CA2352488A1 CA 2352488 A1 CA2352488 A1 CA 2352488A1 CA 002352488 A CA002352488 A CA 002352488A CA 2352488 A CA2352488 A CA 2352488A CA 2352488 A1 CA2352488 A1 CA 2352488A1
Authority
CA
Canada
Prior art keywords
gene
seedling
agrobacterium
cells
selectable marker
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002352488A
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English (en)
Inventor
Maria J. Harrison
Anthony T. Trieu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roberts Samuels Noble Foundation Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2352488A1 publication Critical patent/CA2352488A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Cette invention concerne un procédé de transformation génétique induite par Agrobacterium à partir de graines. Ce procédé peut s'appliquer aux dicotylédones et aux monocotylédones pouvant être transformées par Agrobacterium. Ce procédé fait appel à l'infiltration sous vide pour introduire l'ADN-T d'Agrobacterium porteur du gène d'intérêt dans les graines. Une fois les graines précédemment infiltrées par le gène d'intérêt arrivées à maturité, on les fait germer et on sélectionne les plantes porteuses du transgène. Ce procédé de transformation permet d'obtenir des plantes présentant des caractéristiques stables provenant du transgène sans avoir à faire appel à des procédés de régénération tels que l'embryogenèse somatique ou l'organogenèse.
CA002352488A 1998-12-23 1999-12-23 Procede de transformation de vegetaux Abandoned CA2352488A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US11371798P 1998-12-23 1998-12-23
US60/113,717 1998-12-23
US14537399P 1999-07-23 1999-07-23
US60/145,373 1999-07-23
PCT/US1999/030972 WO2000037663A2 (fr) 1998-12-23 1999-12-23 Procede de transformation de vegetaux

Publications (1)

Publication Number Publication Date
CA2352488A1 true CA2352488A1 (fr) 2000-06-29

Family

ID=26811389

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002352488A Abandoned CA2352488A1 (fr) 1998-12-23 1999-12-23 Procede de transformation de vegetaux

Country Status (5)

Country Link
EP (1) EP1141356A2 (fr)
JP (1) JP2002533090A (fr)
AU (1) AU2594300A (fr)
CA (1) CA2352488A1 (fr)
WO (1) WO2000037663A2 (fr)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1306441B1 (fr) * 2000-08-03 2008-12-17 Japan Tobacco Inc. Procede permettant d'ameliorer l'efficacite du transfert de genes dans des cellules vegetales
CA2386125C (fr) * 2000-08-03 2010-07-13 Japan Tobacco Inc. Methode d'amelioration de l'efficacite du transfert de genes dans des cellules de plantes
CN1149918C (zh) * 2001-02-26 2004-05-19 山西省农业生物技术研究中心 农杆菌介导植物萌发种子基因转化方法
CN1668752A (zh) * 2002-07-16 2005-09-14 独立行政法人农业生物资源研究所 包括减压/增压的使用的电穿孔方法
PL2480658T3 (pl) 2009-09-22 2017-12-29 Medicago Inc. Sposób otrzymywania VLP pochodzenia roślinnego
US20110191903A1 (en) 2009-12-31 2011-08-04 Pioneer Hi-Bred International, Inc. Engineering plant resistance to diseases caused by pathogens
MY180803A (en) 2010-06-02 2020-12-09 Evolva Inc Recombinant production of steviol glycosides
WO2011154159A1 (fr) 2010-06-09 2011-12-15 Bayer Bioscience N.V. Procédés et moyens pour modifier un génome végétal au niveau d'une séquence nucléotidique habituellement utilisée dans l'ingénierie des génomes végétaux
CN103282501A (zh) 2010-11-04 2013-09-04 麦迪卡格公司 植物表达系统
JP2014502840A (ja) * 2010-12-22 2014-02-06 フィリップ・モーリス・プロダクツ・ソシエテ・アノニム 植物のバキュームインフィルトレーション方法およびシステム
TWI620816B (zh) 2011-03-23 2018-04-11 苜蓿股份有限公司 植物衍生蛋白回收方法
CN102212553A (zh) * 2011-04-15 2011-10-12 山西省农业科学院生物技术研究中心 超声波辅助农杆菌介导植物萌发种子基因转化方法
WO2013022989A2 (fr) 2011-08-08 2013-02-14 Evolva Sa Production par recombinaison de glycosides de stéviol
AU2014214004B2 (en) 2013-02-06 2018-05-24 Evolva Sa Methods for improved production of Rebaudioside D and Rebaudioside M
MY184253A (en) 2013-02-11 2021-03-29 Evolva Sa Efficient production of steviol glycosides in recombinant hosts
JP6599354B2 (ja) 2014-03-27 2019-10-30 メディカゴ インコーポレイテッド 改変されたｃｐｍｖエンハンサーエレメント
EP3167057B1 (fr) 2014-07-11 2020-03-25 Medicago Inc. Modification de la production de protéines chez les plantes
SG10201901007PA (en) 2014-08-11 2019-03-28 Evolva Sa Production of steviol glycosides in recombinant hosts
AU2015314251A1 (en) 2014-09-09 2017-03-16 Evolva Sa Production of steviol glycosides in recombinant hosts
US10364450B2 (en) 2015-01-30 2019-07-30 Evolva Sa Production of steviol glycoside in recombinant hosts
US10604743B2 (en) 2015-03-16 2020-03-31 Dsm Ip Assets B.V. UDP-glycosyltransferases
JP2018524345A (ja) 2015-07-02 2018-08-30 メディカゴ インコーポレイテッド ジャスモン酸経路アクチベーター
EP3332018B1 (fr) 2015-08-07 2022-07-27 Evolva SA Production de glycosides de stéviol dans des hôtes recombinants
MY198001A (en) 2016-04-13 2023-07-25 Evolva Sa Production of steviol glycoside in recombinant hosts
WO2017198682A1 (fr) 2016-05-16 2017-11-23 Evolva Sa Production de glycosides de stéviol dans des hôtes de recombinaison
WO2018083338A1 (fr) 2016-11-07 2018-05-11 Evolva Sa Production de glycosides de stéviol dans des hôtes recombinants
CN117479831A (zh) 2021-06-09 2024-01-30 南特知识产权控股有限责任公司 用于在植物中生产感兴趣的蛋白质的方法和系统
CN114657206B (zh) * 2022-03-17 2024-03-22 中国热带农业科学院热带作物品种资源研究所 一种柱花草的遗传转化方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5569597A (en) * 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
WO1989005859A1 (fr) * 1987-12-21 1989-06-29 The Upjohn Company Transformation par l'agrobacterium de graines de plantes de germination
WO1994002620A2 (fr) * 1992-07-27 1994-02-03 Pioneer Hi-Bred International, Inc. Procede ameliore de transformation induite par agrobacterium de cellules de soja cultivees
US5981730A (en) * 1994-04-13 1999-11-09 The General Hospital Corporation RPS gene family, primers, probes, and detection methods
HUP9902123A3 (en) * 1996-06-21 2002-01-28 Monsanto Technology Llc St Louis Methods for the production of stably-transformed, fertile wheat employing agrobacterium-mediated transformation
ID18492A (id) * 1996-09-23 1997-10-16 F B Invest Pty Ltd Pemetaan dan metoda modifikasi
AU762964B2 (en) * 1998-10-15 2003-07-10 Protein Research Trust Transformation process

Also Published As

Publication number Publication date
WO2000037663A3 (fr) 2000-11-09
JP2002533090A (ja) 2002-10-08
AU2594300A (en) 2000-07-12
WO2000037663A2 (fr) 2000-06-29
EP1141356A2 (fr) 2001-10-10

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