CA2368875A1 - High sensitivity phage display biomolecule detection - Google Patents

High sensitivity phage display biomolecule detection Download PDF

Info

Publication number
CA2368875A1
CA2368875A1 CA002368875A CA2368875A CA2368875A1 CA 2368875 A1 CA2368875 A1 CA 2368875A1 CA 002368875 A CA002368875 A CA 002368875A CA 2368875 A CA2368875 A CA 2368875A CA 2368875 A1 CA2368875 A1 CA 2368875A1
Authority
CA
Canada
Prior art keywords
phage
biomolecule
bound
population
collection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002368875A
Other languages
English (en)
French (fr)
Inventor
Carl R. Merril
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Government of the United States of America
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2368875A1 publication Critical patent/CA2368875A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA002368875A 1999-04-14 2000-04-13 High sensitivity phage display biomolecule detection Abandoned CA2368875A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12921599P 1999-04-14 1999-04-14
US60/129,215 1999-04-14
PCT/US2000/010146 WO2000061790A1 (en) 1999-04-14 2000-04-13 High sensitivity phage display biomolecule detection

Publications (1)

Publication Number Publication Date
CA2368875A1 true CA2368875A1 (en) 2000-10-19

Family

ID=22438929

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002368875A Abandoned CA2368875A1 (en) 1999-04-14 2000-04-13 High sensitivity phage display biomolecule detection

Country Status (8)

Country Link
US (1) US6740492B2 (enExample)
EP (1) EP1169473B1 (enExample)
JP (1) JP2002541818A (enExample)
AT (1) ATE346162T1 (enExample)
AU (1) AU778776B2 (enExample)
CA (1) CA2368875A1 (enExample)
DE (1) DE60031965D1 (enExample)
WO (1) WO2000061790A1 (enExample)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2360282A (en) * 2000-03-17 2001-09-19 Bioinvent Int Ab Making and using micro-arrays of biological materials
WO2003010198A1 (en) * 2001-07-26 2003-02-06 Kenton Srl Identification of specific tumour antigens by selection of cdna libraries with sera and use of said antigens in diagnostic techniques
WO2003011902A1 (en) * 2001-07-27 2003-02-13 Kenton S.R.L. Identification of specific tumour antigens by selection of cdna libraries with sera
JP4601427B2 (ja) * 2002-09-18 2010-12-22 ザ・トラステイーズ・オブ・ザ・ユニバーシテイ・オブ・ペンシルベニア 細胞上および生物学的混合物中の抗原の検出のための組成物、方法およびキット
GB0304832D0 (en) * 2003-03-04 2003-04-09 Secr Defence Assay method
EP1853696B1 (en) * 2005-02-24 2019-04-17 Life Technologies (Israel) Ltd. Electro-blotting devices, systems, and kits, and methods for their use
US20080193917A1 (en) 2005-05-27 2008-08-14 David Alexander Schofield Biological Detection System and Method
US8066866B2 (en) * 2005-10-17 2011-11-29 Lifescan, Inc. Methods for measuring physiological fluids
EP1963494B1 (en) * 2005-11-29 2014-10-08 The Trustees of The University of Pennsylvania Phage particle diagnostic reagents
CN102150045A (zh) * 2008-07-11 2011-08-10 生命技术公司 增强型电泳检测固相支持物上的分析物
USD738527S1 (en) 2013-05-28 2015-09-08 Life Technologies Corporation Electroblotting apparatus
TWI570243B (zh) * 2015-11-03 2017-02-11 國立清華大學 噬菌體之篩選裝置及方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
GB9015198D0 (en) 1990-07-10 1990-08-29 Brien Caroline J O Binding substance
GB9104411D0 (en) * 1991-03-01 1991-04-17 Elchrom Limited Improvement of gel composition in gels for submerged gel electrophoresis
BE1006312A3 (fr) * 1991-11-29 1994-07-19 Univ Catholique Louvain Procede de selection de microorganismes recombinants comportant a leur surface au moins une molecule a activite enzymatique.
US5679510A (en) * 1993-04-27 1997-10-21 Hybridon, Inc. Quantitative detection of specific nucleic acid sequences using lambdoid bacteriophages linked by oligonucleotides to solid support
US5650267A (en) * 1993-04-27 1997-07-22 Symbiotech, Inc. Method of detecting compounds utilizing genetically modified lambdoid bacteriophage
US6027877A (en) * 1993-11-04 2000-02-22 Gene Check, Inc. Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, purification of amplified DNA samples and allele identification
US5702892A (en) 1995-05-09 1997-12-30 The United States Of America As Represented By The Department Of Health And Human Services Phage-display of immunoglobulin heavy chain libraries
IL114149A0 (en) * 1995-06-14 1995-10-31 Yeda Res & Dev Modified avidin and streptavidin molecules and use thereof
AU1127097A (en) * 1995-12-15 1997-07-14 Pharmacia & Upjohn Company Method for the simultaneous detection of a therapeutic target for a disease state and its neutralizing antibody-like molecule
EP0934526B1 (en) * 1996-10-08 2003-01-08 U-BISys B.V. Methods and means for selecting peptides and proteins having specific affinity for a target

Also Published As

Publication number Publication date
EP1169473B1 (en) 2006-11-22
US6740492B2 (en) 2004-05-25
AU4461200A (en) 2000-11-14
AU778776B2 (en) 2004-12-23
US20020110806A1 (en) 2002-08-15
EP1169473A1 (en) 2002-01-09
DE60031965D1 (de) 2007-01-04
WO2000061790A1 (en) 2000-10-19
JP2002541818A (ja) 2002-12-10
ATE346162T1 (de) 2006-12-15

Similar Documents

Publication Publication Date Title
JP3143477B2 (ja) 生物学的サンプル中における生物学的分子の検出のためのバクテリオファージの使用に基づく方法
AU778776B2 (en) High sensitivity phage display biomolecule detection
FI121014B (fi) Sinkkisormiproteiinijohdannaisia ja niiden menetelmiä
JP2780773B2 (ja) サルモネラ検定法
Christmann et al. Epitope mapping and affinity purification of monospecific antibodies by Escherichia coli cell surface display of gene-derived random peptide libraries
JP4276301B2 (ja) 固定化プローブを用いる、分子の電気泳動分析
Lee et al. Interaction of gp16 with pRNA and DNA for genome packaging by the motor of bacterial virus phi29
WO1995034683A1 (en) Method of detecting compounds utilizing genetically modified lambdoid bacteriophage
Bialek et al. Epitope-targeted proteome analysis: towards a large-scale automated protein–protein-interaction mapping utilizing synthetic peptide arrays
US20030211532A1 (en) Methods and compositions for analyzing nucleic acids
JP2003508015A (ja) アダプター分子を用いる標的分子の電気泳動分析
US20100240547A1 (en) Methods for selecting protein binding moieties
Qian et al. Cloning of a cDNA encoding an RNA binding protein by screening expression libraries using a northwestern strategy
JP2001505417A (ja) 生物の成長に必須の遺伝子の同定方法
Cohen et al. Multifunctional roles of a bacteriophage ϕ29 morphogenetic factor in assembly and infection
US20050239078A1 (en) Sequence tag microarray and method for detection of multiple proteins through DNA methods
JP2004512500A (ja) 分子形状の識別方法
EP0401313B1 (en) Macromolecular conjugate
CN120699099A (zh) 一种靶向usp7的多肽及多肽-蛋白水解靶向嵌合体
CN117660460A (zh) 一种结合人rest协阻抑因子3蛋白的核酸适配体及其应用
JP2001510553A (ja) 組換え蛋白質を用いたｈｔｌｖ抗体の検出法
JPH07505999A (ja) 表面に，酵素活性を有する少なくとも１個の分子を含む組換え微生物を選択する方法
US20090203548A1 (en) Complex able to detect an analyte, method for its preparation and uses thereof
JP2002507275A (ja) 引き算ライブラリーの製造方法
JPH03128000A (ja) 核酸の検出法及び検出試薬

Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued