CA2331150A1 - Procede industriel de production de proteines heterologues chez e. coli et souches utiles pour le procede - Google Patents

Procede industriel de production de proteines heterologues chez e. coli et souches utiles pour le procede Download PDF

Info

Publication number
CA2331150A1
CA2331150A1 CA002331150A CA2331150A CA2331150A1 CA 2331150 A1 CA2331150 A1 CA 2331150A1 CA 002331150 A CA002331150 A CA 002331150A CA 2331150 A CA2331150 A CA 2331150A CA 2331150 A1 CA2331150 A1 CA 2331150A1
Authority
CA
Canada
Prior art keywords
prpa
strain
coli
plasmid
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002331150A
Other languages
English (en)
French (fr)
Inventor
Jerome Pierrard
Carole Guitton
Olivier Favre-Bulle
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Adisseo France SAS
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2331150A1 publication Critical patent/CA2331150A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
CA002331150A 1998-06-10 1999-06-08 Procede industriel de production de proteines heterologues chez e. coli et souches utiles pour le procede Abandoned CA2331150A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR98/07474 1998-06-10
FR9807474A FR2780416B1 (fr) 1998-06-10 1998-06-10 Procede industriel de production de proteines heterologues chez e. coli et souches utiles pour le procede
PCT/FR1999/001343 WO1999064607A1 (fr) 1998-06-10 1999-06-08 Procede industriel de production de proteines heterologues chez e. coli et souches utiles pour le procede

Publications (1)

Publication Number Publication Date
CA2331150A1 true CA2331150A1 (fr) 1999-12-16

Family

ID=9527357

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002331150A Abandoned CA2331150A1 (fr) 1998-06-10 1999-06-08 Procede industriel de production de proteines heterologues chez e. coli et souches utiles pour le procede

Country Status (8)

Country Link
EP (1) EP1084258A1 (ru)
JP (1) JP2002517252A (ru)
AU (1) AU753879B2 (ru)
CA (1) CA2331150A1 (ru)
EA (1) EA200100023A1 (ru)
FR (1) FR2780416B1 (ru)
IL (1) IL140073A0 (ru)
WO (1) WO1999064607A1 (ru)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2822460B1 (fr) * 2001-03-26 2005-04-29 Rhodia Chimie Sa Procede de preparation enantioselectif d'acides carboxyliques optiquement actifs par hydrolyse enzymatique de nitriles
WO2003085108A1 (fr) * 2002-04-10 2003-10-16 Ajinomoto Co., Inc. Adn recombinant presentant un gene d'hydantoinase et un gene de carbamylase et procede de production d'acide amine
EP1599584B1 (en) 2003-02-27 2009-07-01 Basf Se Modified nitrilases and their use in methods for the production of carboxylic acids
JP5808526B2 (ja) * 2010-04-09 2015-11-10 三洋化成工業株式会社 有用物質製造方法
JP5808527B2 (ja) * 2010-04-09 2015-11-10 三洋化成工業株式会社 有用物質製造方法
JP5808529B2 (ja) * 2010-06-28 2015-11-10 三洋化成工業株式会社 有用物質生産方法
JP5808530B2 (ja) * 2010-06-28 2015-11-10 三洋化成工業株式会社 有用物質生産方法
CN104254612B (zh) 2012-04-26 2018-06-01 安迪苏法国联合股份有限公司 一种2,4-二羟基丁酸的生产方法
BR112015000534B1 (pt) 2012-07-11 2023-02-28 Adisseo France S.A.S Método para a preparação de 2,4-dihidroxibutirato, microrganismo modificado e método de produção de 2,4-dhb
KR20150045432A (ko) 2012-07-11 2015-04-28 엥스티튀 나쇼날 데 샹스 아플리케 드 툴루즈 변형된 1,3-프로판올 생산 미생물

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2209591A1 (de) * 1971-03-04 1972-09-14 Asahi Kasei Kogyo K.K., Osaka (Japan) Verfahren zur biotechnischen Herstellung von L-5-Hydroxytryptophan
US4246346A (en) * 1978-09-05 1981-01-20 Aktiebolaget Fermenta Antibiotic and steroid transformation process
US5000000A (en) * 1988-08-31 1991-03-19 University Of Florida Ethanol production by Escherichia coli strains co-expressing Zymomonas PDC and ADH genes
GB8821795D0 (en) * 1988-09-16 1988-10-19 Erba Carlo Spa New derivatives of human/bovine basic fibroplast growth factor
ES2020792A6 (es) * 1990-08-03 1991-09-16 Antibioticos Sa Un procedimiento enzimatico para la presentacion de acido 7-amino-cefa-losporanico y un procedimiento para expresar la enzima 7b (4-carboxibutanamido) cefalosporinacilasa.
IT1252308B (it) * 1990-12-21 1995-06-08 Antibioticos Spa Procedimento enzimatico per la produzione di acido 7- amminocefalosporanico e derivati
FR2694571B1 (fr) * 1992-08-10 1994-11-04 Rhone Poulenc Chimie Polypeptides possédant une activité nitrilase, séquence d'ADN codant pour lesdits polypeptides, cassettes d'expression et micro-organismes hôtes permettant leur obtention.
JP3880636B2 (ja) * 1994-01-10 2007-02-14 味の素株式会社 発酵法によるｌ−グルタミン酸の製造法
FR2755143B1 (fr) * 1996-10-25 1998-11-27 Rhone Poulenc Nutrition Animal Procede de preparation de l'acide 2-hydroxy-4-methylthio-butyrique par utilisation d'une nitrilase

Also Published As

Publication number Publication date
FR2780416A1 (fr) 1999-12-31
IL140073A0 (en) 2002-02-10
FR2780416B1 (fr) 2002-12-20
AU753879B2 (en) 2002-10-31
WO1999064607A1 (fr) 1999-12-16
EP1084258A1 (fr) 2001-03-21
AU4046699A (en) 1999-12-30
EA200100023A1 (ru) 2001-06-25
JP2002517252A (ja) 2002-06-18

Similar Documents

Publication Publication Date Title
EP0596812B1 (fr) Nitrilase recombinante et son utilisation
US20070117191A1 (en) Reductase gene for alpha-substituted-alpha, beta-unsaturated carbonyl compound
CA2433485A1 (en) Method for fermentative production of amino acids and amino acid derivatives of the phosphoglycerate family
JP4402587B2 (ja) α−Ｈ−α−アミノ酸アミドラセマーゼ活性を有するポリペプチドおよびそれをコードする核酸
CA2331150A1 (fr) Procede industriel de production de proteines heterologues chez e. coli et souches utiles pour le procede
CA2449836C (fr) Preparation stereoselective de l-acides amines cycliques
JP2013510587A (ja) 全細胞の生体触媒
JP3408737B2 (ja) ニトリルヒドラターゼの活性化に関与するタンパク質及びそれをコードする遺伝子
WO1991011518A1 (fr) Polypeptides impliques dans la biosynthese des cobalamines et/ou des cobamides, sequences d'adn codant pour ces polypeptides, procede de preparation, et leur utilisation
CN113652408B (zh) 羰基还原酶突变体及其在(r)-4-氯-3-羟基丁酸乙酯合成中的应用
TW200426218A (en) Method for the fermentative production of R-α-lipoic acid
CA2372097C (fr) Epoxyde hydrolases d'origine aspergillus
Lee et al. Mass production of thermostable D‐hydantoinase by batch culture of recombinant Escherichia coli with a constitutive expression system
JP4118687B2 (ja) Ａｒｔｈｒｏｂａｃｔｅｒ ｃｒｙｓｔａｌｌｏｐｏｉｅｔｅｓ（アリスロバクテリア クリスタロポイテス）ＤＳＭ２０１１７株由来のＤ−カルバモイラーゼ
WO2000036120A1 (fr) Methode d'isolement et de selection de genes codant pour des enzymes, et milieu de culture approprie
EP1071798B1 (fr) Constructions pour l'expression controlee de genes recombinants dans des cellules procaryotes
JP3541949B2 (ja) メチロトローフ酵母におけるグリコレートオキシダーゼの製造
FR2728905A1 (fr) Nouvelle acide amine amidohydrolase, sequence nuclotidique correspondant et leurs utilisations
FR2715167A1 (fr) Procédé de production de la L-carnitine par voie microbiologique.
FR2700777A1 (fr) Polypeptides à activité amidase, outils génétiques et micro-organismes hôtes permettant leur obtention, procédé d'hydrolyse mettant en Óoeuvre lesdits polypeptides.
JP2023527107A (ja) シスチンを生体触媒還元するための酵素的触媒酸化還元系のコア構成成分としての生体触媒
KR101124617B1 (ko) 신규 리포산 합성효소와 리포산 단백질 리가제를 이용한 알파-리포산의 생산방법
JPWO2003085108A1 (ja) ヒダントイナーゼ遺伝子及びカルバミラーゼ遺伝子を有する組換えｄｎａ、並びにアミノ酸製造方法
WO1992005253A1 (fr) Procede microbiologique de preparation de l'apolipoproteine aiv humaine ou de derives de celle-ci
JP2002330784A (ja) ５置換ヒダントインラセマーゼ、これをコードするｄｎａ、組み換えｄｎａ、形質転換された細胞および光学活性アミノ酸の製造方法

Legal Events

Date Code Title Description
FZDE Discontinued