CA2319021A1 - Procede et dispositif de lyse cellulaire - Google Patents

Procede et dispositif de lyse cellulaire Download PDF

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Publication number
CA2319021A1
CA2319021A1 CA002319021A CA2319021A CA2319021A1 CA 2319021 A1 CA2319021 A1 CA 2319021A1 CA 002319021 A CA002319021 A CA 002319021A CA 2319021 A CA2319021 A CA 2319021A CA 2319021 A1 CA2319021 A1 CA 2319021A1
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CA
Canada
Prior art keywords
tubing
meeting point
stream
agent
mixture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002319021A
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English (en)
Inventor
Michel Chevalier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
AVENTIS PASTEUR
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2319021A1 publication Critical patent/CA2319021A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Procédé et dispositif de lyse cellulaire dans lequel on réalise, en continu, un mélange liquide de bactéries ou de cellules eucaryotes et d'un agent de lyse, et l'on fait immédiatement écouler ce mélange dans un flux constant à l'intérieur d'une canalisation (7), le débit de ce flux étant adapté, en fonction du diamètre et de la longueur de la canalisation (7), de manière à obtenir un lysat cellulaire sensiblement homogène à la sortie (8) de ladite canalisation.
CA002319021A 1998-01-21 1999-01-20 Procede et dispositif de lyse cellulaire Abandoned CA2319021A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR98/00815 1998-01-21
FR9800815A FR2773818B1 (fr) 1998-01-21 1998-01-21 Procede et dispositif de lyse de bacteries
PCT/FR1999/000105 WO1999037750A1 (fr) 1998-01-21 1999-01-20 Procede et dispositif de lyse cellulaire

Publications (1)

Publication Number Publication Date
CA2319021A1 true CA2319021A1 (fr) 1999-07-29

Family

ID=9522178

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002319021A Abandoned CA2319021A1 (fr) 1998-01-21 1999-01-20 Procede et dispositif de lyse cellulaire

Country Status (7)

Country Link
EP (1) EP1049766A1 (fr)
JP (1) JP2002500878A (fr)
AU (1) AU756179B2 (fr)
CA (1) CA2319021A1 (fr)
FR (1) FR2773818B1 (fr)
NZ (1) NZ505865A (fr)
WO (1) WO1999037750A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1593741B1 (fr) * 2004-05-03 2009-01-07 PlasmidFactory GmbH & Co. KG Procédé pour la production de molécules d'acides nucléiques extra-chromosomiques

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6664049B1 (en) 1999-01-20 2003-12-16 Aventis Pasteur S.A. Method and device for cell lysis
CN1134545C (zh) * 1999-10-02 2004-01-14 株式会社百尼尔 自动化dna提纯装置
KR100470803B1 (ko) * 2002-09-25 2005-03-10 대한민국(관리부서:국립수산과학원) 형질전환 어류의 피씨알 분석을 위한 신속한 디앤에이 추출 방법
US8501402B2 (en) 2003-03-24 2013-08-06 Boehringer Ingelheim Rcv Gmbh & Co Kg Methods and devices for producing biomolecules
WO2005100542A1 (fr) * 2004-04-19 2005-10-27 Centelion Procede destine a purifier de l'adn plasmidique
BRPI0413907A (pt) * 2003-09-17 2006-10-24 Centelion método de preparação de plasmìdeo dna de grau farmacêutico
BRPI0515553A (pt) * 2004-09-17 2008-07-29 Centelion formulações lìquidas estáveis de dna de plasmìdeo
WO2008019964A1 (fr) * 2006-08-15 2008-02-21 Basf Se Procédé permettant d'isoler des protéines des cellules de production
US8211668B2 (en) * 2007-01-12 2012-07-03 Dow Agrosciences, Llc. Apparatus and methods for osmotically shocking cells
EP2088196A1 (fr) 2008-02-08 2009-08-12 Boehringer Ingelheim RCV GmbH & Co KG Procédés et dispositifs pour la production de biomolécules
US8722329B2 (en) * 2011-02-21 2014-05-13 Rheonix, Inc. Microfluidic device-based nucleic acid purification method
EP3594338A1 (fr) 2018-07-12 2020-01-15 Kaneka Eurogentec SA Procédé et appareil pour la purification de séquences d'acides nucléiques extra-chromosomiques
WO2021198281A1 (fr) * 2020-03-31 2021-10-07 Richter-Helm Biologics Gmbh & Co. Kg Procédés de production d'adn plasmidique
CN111979109A (zh) * 2020-09-01 2020-11-24 深圳普瑞金生物药业有限公司 质粒载体连续裂解装置

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1579120A (en) * 1975-08-05 1980-11-12 Union International Co Ltd Extracts of the haemopoietic system
DE4028771A1 (de) * 1989-09-14 1991-02-21 Bert Sobolewski Verfahren zur trennung von parasiten, organellen o. dgl. von den sie umgebenden biologischen membranen
US5096818A (en) * 1990-06-04 1992-03-17 Autogen Instruments, Inc. Nucleic acid separation method
JPH10503086A (ja) * 1994-07-15 1998-03-24 メルク エンド カンパニー インコーポレーテッド 大規模プラスミド精製方法
ATE268810T1 (de) * 1995-12-21 2004-06-15 Genzyme Corp Methode zur zellyse

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1593741B1 (fr) * 2004-05-03 2009-01-07 PlasmidFactory GmbH & Co. KG Procédé pour la production de molécules d'acides nucléiques extra-chromosomiques

Also Published As

Publication number Publication date
FR2773818A1 (fr) 1999-07-23
AU2060499A (en) 1999-08-09
AU756179B2 (en) 2003-01-09
WO1999037750A1 (fr) 1999-07-29
EP1049766A1 (fr) 2000-11-08
FR2773818B1 (fr) 2000-02-18
NZ505865A (en) 2002-12-20
JP2002500878A (ja) 2002-01-15

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued