NZ505865A - Method for cell lysis continuously producing a mixture of nucleated cells and lysing agent , the mixture flows in a steady stream through a tube having a diameter of 1cm or less - Google Patents

Method for cell lysis continuously producing a mixture of nucleated cells and lysing agent , the mixture flows in a steady stream through a tube having a diameter of 1cm or less

Info

Publication number
NZ505865A
NZ505865A NZ505865A NZ50586599A NZ505865A NZ 505865 A NZ505865 A NZ 505865A NZ 505865 A NZ505865 A NZ 505865A NZ 50586599 A NZ50586599 A NZ 50586599A NZ 505865 A NZ505865 A NZ 505865A
Authority
NZ
New Zealand
Prior art keywords
mixture
diameter
cell lysis
tube
less
Prior art date
Application number
NZ505865A
Inventor
Michel Chevalier
Original Assignee
Aventis Pasteur
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aventis Pasteur filed Critical Aventis Pasteur
Publication of NZ505865A publication Critical patent/NZ505865A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A method and a device for cell lysis which continuously produces a liquid mixture of bacteria or erkaryiotic cells and a lysis agent, and in immediately caused said mixture to flow in a constant flux inside a pipe (7), the flow rate of said flux being adapted , according to the pipe (7) length and diameter, so as to obtain a substantially homogenous lysate at said pipe (7) outlet.
NZ505865A 1998-01-21 1999-01-20 Method for cell lysis continuously producing a mixture of nucleated cells and lysing agent , the mixture flows in a steady stream through a tube having a diameter of 1cm or less NZ505865A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR9800815A FR2773818B1 (en) 1998-01-21 1998-01-21 BACTERIA LYSIS PROCESS AND DEVICE
PCT/FR1999/000105 WO1999037750A1 (en) 1998-01-21 1999-01-20 Method and device for cell lysis

Publications (1)

Publication Number Publication Date
NZ505865A true NZ505865A (en) 2002-12-20

Family

ID=9522178

Family Applications (1)

Application Number Title Priority Date Filing Date
NZ505865A NZ505865A (en) 1998-01-21 1999-01-20 Method for cell lysis continuously producing a mixture of nucleated cells and lysing agent , the mixture flows in a steady stream through a tube having a diameter of 1cm or less

Country Status (7)

Country Link
EP (1) EP1049766A1 (en)
JP (1) JP2002500878A (en)
AU (1) AU756179B2 (en)
CA (1) CA2319021A1 (en)
FR (1) FR2773818B1 (en)
NZ (1) NZ505865A (en)
WO (1) WO1999037750A1 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6664049B1 (en) 1999-01-20 2003-12-16 Aventis Pasteur S.A. Method and device for cell lysis
WO2001025482A1 (en) * 1999-10-02 2001-04-12 Bioneer Corporation Automatic dna purification apparatus
KR100470803B1 (en) * 2002-09-25 2005-03-10 대한민국(관리부서:국립수산과학원) A rapid dna extraction method for pcr-based analysis of transgenic fish
US8501402B2 (en) 2003-03-24 2013-08-06 Boehringer Ingelheim Rcv Gmbh & Co Kg Methods and devices for producing biomolecules
JP2007505613A (en) * 2003-09-17 2007-03-15 センテリオン Method for preparing pharmaceutical grade plasmid DNA
KR20060135062A (en) * 2004-04-19 2006-12-28 센텔리옹 에스아에스 Method for purifying plasmid dna
DE102004052254A1 (en) * 2004-05-03 2005-12-01 Plasmid Factory Gmbh & Co. Kg Process for disrupting cells and separating cell components
EA011554B1 (en) * 2004-09-17 2009-04-28 Сентелион Stable liquid formulations of plasmid dna
JP2010500042A (en) * 2006-08-15 2010-01-07 ビーエーエスエフ ソシエタス・ヨーロピア Method for protein isolation from producer cells
CN101646780A (en) * 2007-01-12 2010-02-10 陶氏环球技术公司 The apparatus and method for of osmotically shocking cells
EP2088196A1 (en) 2008-02-08 2009-08-12 Boehringer Ingelheim RCV GmbH & Co KG Methods and devices for producing biomolecules
EP2678429B1 (en) * 2011-02-21 2018-04-18 Rheonix, Inc. Microfluidic device-based nucleic acid purification method
EP3594338A1 (en) 2018-07-12 2020-01-15 Kaneka Eurogentec SA Method and apparatus for the purification of extra-chromosomal nucleic acid sequences
WO2021198281A1 (en) * 2020-03-31 2021-10-07 Richter-Helm Biologics Gmbh & Co. Kg Methods for producing plasmid dna
CN111979109A (en) * 2020-09-01 2020-11-24 深圳普瑞金生物药业有限公司 Plasmid vector continuous cracking device

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1579120A (en) * 1975-08-05 1980-11-12 Union International Co Ltd Extracts of the haemopoietic system
DE4028771A1 (en) * 1989-09-14 1991-02-21 Bert Sobolewski Stripping membrane coverings from parasites by shear force - developed during turbulent flow in restricted bore
US5096818A (en) * 1990-06-04 1992-03-17 Autogen Instruments, Inc. Nucleic acid separation method
WO1996002658A1 (en) * 1994-07-15 1996-02-01 Merck & Co., Inc. A method for large scale plasmid purification
CA2212446C (en) * 1995-12-21 2010-09-21 Genzyme Corporation Method for lysing cells

Also Published As

Publication number Publication date
EP1049766A1 (en) 2000-11-08
AU2060499A (en) 1999-08-09
FR2773818B1 (en) 2000-02-18
JP2002500878A (en) 2002-01-15
AU756179B2 (en) 2003-01-09
CA2319021A1 (en) 1999-07-29
FR2773818A1 (en) 1999-07-23
WO1999037750A1 (en) 1999-07-29

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Legal Events

Date Code Title Description
ASS Change of ownership

Owner name: AVENTIS PASTEUR, FR

Free format text: OLD OWNER(S): PASTEUR MERIEUX SERUMS ET VACCINS S A

RENW Renewal (renewal fees accepted)
PSEA Patent sealed