CA2280196C - Chimeric genes and methods for increasing the lysine content of the seeds of plants - Google Patents

Chimeric genes and methods for increasing the lysine content of the seeds of plants Download PDF

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Publication number
CA2280196C
CA2280196C CA2280196A CA2280196A CA2280196C CA 2280196 C CA2280196 C CA 2280196C CA 2280196 A CA2280196 A CA 2280196A CA 2280196 A CA2280196 A CA 2280196A CA 2280196 C CA2280196 C CA 2280196C
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CA
Canada
Prior art keywords
lysine
gene
seq
seeds
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2280196A
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English (en)
French (fr)
Other versions
CA2280196A1 (en
Inventor
Saverio Carl Falco
Raymond Ervin Mcdevitt Iii
Sabine Ursula Epelbaum
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EIDP Inc
Original Assignee
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Publication of CA2280196A1 publication Critical patent/CA2280196A1/en
Application granted granted Critical
Publication of CA2280196C publication Critical patent/CA2280196C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8254—Tryptophan or lysine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nutrition Science (AREA)
  • Reproductive Health (AREA)
  • Pregnancy & Childbirth (AREA)
  • Developmental Biology & Embryology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pretreatment Of Seeds And Plants (AREA)
CA2280196A 1997-03-27 1998-03-27 Chimeric genes and methods for increasing the lysine content of the seeds of plants Expired - Fee Related CA2280196C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US82462797A 1997-03-27 1997-03-27
US08/824,627 1997-03-27
PCT/US1998/006051 WO1998042831A2 (en) 1997-03-27 1998-03-27 Chimeric genes and methods for increasing the lysine content of the seeds of plants

Publications (2)

Publication Number Publication Date
CA2280196A1 CA2280196A1 (en) 1998-10-01
CA2280196C true CA2280196C (en) 2012-05-15

Family

ID=25241893

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2280196A Expired - Fee Related CA2280196C (en) 1997-03-27 1998-03-27 Chimeric genes and methods for increasing the lysine content of the seeds of plants

Country Status (12)

Country Link
EP (1) EP0973880A2 (ja)
JP (1) JP2001502923A (ja)
KR (1) KR20010005645A (ja)
CN (1) CN1253584A (ja)
AU (1) AU747997B2 (ja)
BR (1) BR9811256A (ja)
CA (1) CA2280196C (ja)
HU (1) HUP0002305A3 (ja)
ID (1) ID22486A (ja)
PL (1) PL336042A1 (ja)
TR (1) TR199902349T2 (ja)
WO (1) WO1998042831A2 (ja)

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EP1074629A1 (en) * 1999-07-30 2001-02-07 Coöperatieve Verkoop- en Productievereniging van Aardappelmeel en Derivaten 'AVEBE' B.A. Sink protein
US7157281B2 (en) 2003-12-11 2007-01-02 Monsanto Technology Llc High lysine maize compositions and event LY038 maize plants
AR047598A1 (es) * 2004-02-10 2006-01-25 Monsanto Technology Llc Semilla de maiz transgenica con mayor contenido de aminoacidos
US7855323B2 (en) 2004-02-10 2010-12-21 Monsanto Technology Llc Recombinant DNA for gene suppression
CN101321873B (zh) * 2005-10-03 2013-08-14 孟山都技术有限公司 具有增加的赖氨酸的转基因植物种子
CN100412198C (zh) * 2006-04-05 2008-08-20 北京凯拓迪恩生物技术研发中心有限责任公司 一种提高水稻种子中赖氨酸含量的方法及其专用载体
US7964774B2 (en) 2008-05-14 2011-06-21 Monsanto Technology Llc Plants and seeds of spring canola variety SCV384196
US8466342B2 (en) 2009-06-09 2013-06-18 Pioneer Hi Bred International Inc Early endosperm promoter and methods of use
MX2012004819A (es) 2009-10-26 2012-06-25 Pioneer Hi Bred Int Promotor especifico de ovulo somatico y metodos de uso.
CN102051376B (zh) * 2010-01-27 2013-03-20 华中农业大学 基于油菜子叶叶绿体转化的组织培养体系及获得转化植株的方法
WO2013096818A1 (en) 2011-12-21 2013-06-27 The Curators Of The University Of Missouri Soybean variety s05-11268
WO2013096810A1 (en) 2011-12-21 2013-06-27 The Curators Of The University Of Missouri Soybean variety s05-11482
WO2013103365A1 (en) 2012-01-06 2013-07-11 Pioneer Hi-Bred International, Inc. Pollen preferred promoters and methods of use
EP2800816A1 (en) 2012-01-06 2014-11-12 Pioneer Hi-Bred International Inc. Ovule specific promoter and methods of use
US8878009B2 (en) 2012-04-26 2014-11-04 Monsanto Technology, LLP Plants and seeds of spring canola variety SCV318181
US8859857B2 (en) 2012-04-26 2014-10-14 Monsanto Technology Llc Plants and seeds of spring canola variety SCV259778
US8835720B2 (en) 2012-04-26 2014-09-16 Monsanto Technology Llc Plants and seeds of spring canola variety SCV967592
WO2014059155A1 (en) 2012-10-11 2014-04-17 Pioneer Hi-Bred International, Inc. Guard cell promoters and uses thereof
CA2905743C (en) 2013-03-13 2021-09-28 Pioneer Hi-Bred International, Inc. Glyphosate application for weed control in brassica
EP3744727A1 (en) 2013-03-14 2020-12-02 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
RU2015143825A (ru) 2013-03-15 2017-04-26 Пайонир Хай-Бред Интернэшнл, Инк. Полипептиды phi-4 и способы их применения
EA030896B1 (ru) 2013-08-16 2018-10-31 Пайонир Хай-Бред Интернэшнл, Инк. Инсектицидные белки и способы их применения
ES2937045T3 (es) 2013-09-13 2023-03-23 Pioneer Hi Bred Int Proteínas insecticidas y métodos para su uso
RU2688486C2 (ru) 2014-01-16 2019-05-21 Калиста, Инк. Микроорганизмы для повышенного продуцирования аминокислот и связанные с ними способы
UA120608C2 (uk) 2014-02-07 2020-01-10 Піонір Хай-Бред Інтернешнл, Інк. Очищений поліпептид ptip-83 та спосіб його застосування
US20170218384A1 (en) 2014-08-08 2017-08-03 Pioneer Hi-Bred International, Inc. Ubiquitin promoters and introns and methods of use
US20170247719A1 (en) 2014-09-17 2017-08-31 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
WO2016061206A1 (en) 2014-10-16 2016-04-21 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
US20170359965A1 (en) 2014-12-19 2017-12-21 E I Du Pont De Nemours And Company Polylactic acid compositions with accelerated degradation rate and increased heat stability
BR112017024948A2 (pt) 2015-05-19 2018-07-31 Pioneer Hi Bred Int proteínas inseticidas e métodos para uso das mesmas
MX2017016119A (es) 2015-06-16 2018-03-07 Pioneer Hi Bred Int Composiciones y metodos para controlar plagas de insectos.
BR112018002535A2 (pt) 2015-08-06 2018-09-25 Du Pont polipeptídeo inseticida recombinante, polinucleotídeo recombinante, construto de dna, planta transgênica ou célula de planta, composição, proteína de fusão, método para controlar uma praga, método para inibir crescimento ou para exterminar uma população de pragas ou uma praga e uso do polipeptídeo
AU2016315655A1 (en) 2015-08-28 2018-02-01 E. I. Du Pont De Nemours And Company Ochrobactrum-mediated transformation of plants
CN108575091A (zh) 2015-12-18 2018-09-25 先锋国际良种公司 杀昆虫蛋白及其使用方法
BR112018012887B1 (pt) 2015-12-22 2024-02-06 Pioneer Hi-Bred International, Inc Cassete de expressão, vetor, métodos de obtenção de célula vegetal e planta transgênica, métodos para expressar um polinucleotídeo
CN109068660B (zh) 2016-05-04 2023-05-02 先锋国际良种公司 杀昆虫蛋白及其使用方法
EP3472323A1 (en) 2016-06-16 2019-04-24 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
WO2017222821A2 (en) 2016-06-24 2017-12-28 Pioneer Hi-Bred International, Inc. Plant regulatory elements and methods of use thereof
WO2018005411A1 (en) 2016-07-01 2018-01-04 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
WO2018013333A1 (en) 2016-07-12 2018-01-18 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
CN116003539A (zh) 2016-11-01 2023-04-25 先锋国际良种公司 杀昆虫蛋白及其使用方法
EP3688171A1 (en) 2017-09-25 2020-08-05 Pioneer Hi-Bred International, Inc. Tissue-preferred promoters and methods of use
WO2019226508A1 (en) 2018-05-22 2019-11-28 Pioneer Hi-Bred International, Inc. Plant regulatory elements and methods of use thereof
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EP3874050A1 (en) 2018-10-31 2021-09-08 Pioneer Hi-Bred International, Inc. Compositions and methods for ochrobactrum-mediated plant transformation
CA3186978A1 (en) 2020-07-14 2022-01-20 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
CN114317557B (zh) * 2022-01-06 2023-07-07 河南农业大学 玉米ZmRIBA1基因在高赖氨酸玉米育种中的应用

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EP0734445B1 (en) * 1993-11-30 2006-07-05 E.I. Du Pont De Nemours And Company Chimeric genes and methods for increasing the lysine content of the seeds of corn, soybean and rapeseed plants
JP3716427B2 (ja) * 1994-06-14 2005-11-16 味の素株式会社 α−ケトグルタル酸デヒドロゲナーゼ遺伝子
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Also Published As

Publication number Publication date
HUP0002305A3 (en) 2002-09-30
TR199902349T2 (xx) 2000-01-21
HUP0002305A2 (hu) 2000-09-28
PL336042A1 (en) 2000-06-05
WO1998042831A2 (en) 1998-10-01
EP0973880A2 (en) 2000-01-26
BR9811256A (pt) 2000-07-18
KR20010005645A (ko) 2001-01-15
AU747997B2 (en) 2002-05-30
AU6780198A (en) 1998-10-20
WO1998042831A3 (en) 1998-12-10
JP2001502923A (ja) 2001-03-06
CA2280196A1 (en) 1998-10-01
ID22486A (id) 1999-10-21
CN1253584A (zh) 2000-05-17

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EEER Examination request
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Effective date: 20170327