BR9811256A - Fragmento de ácido nucleico, gene quimérico, célula vegetal, semente de planta, método para reduzir a atividade da lisina cetoglutarato redutase em uma semente de planta e planta - Google Patents

Fragmento de ácido nucleico, gene quimérico, célula vegetal, semente de planta, método para reduzir a atividade da lisina cetoglutarato redutase em uma semente de planta e planta

Info

Publication number
BR9811256A
BR9811256A BR9811256-2A BR9811256A BR9811256A BR 9811256 A BR9811256 A BR 9811256A BR 9811256 A BR9811256 A BR 9811256A BR 9811256 A BR9811256 A BR 9811256A
Authority
BR
Brazil
Prior art keywords
plant
plant seed
seed
lysine
chimeric gene
Prior art date
Application number
BR9811256-2A
Other languages
English (en)
Inventor
Saverio Carl Falco
Raymond Ervin Mcdevitt Iii
Sabine Ursula Epelbaum
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of BR9811256A publication Critical patent/BR9811256A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8254—Tryptophan or lysine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Abstract

"FRAGMENTO DE áCIDO NUCLEICO, GENE QUIMéRICO, CéLULA VEGETAL, SEMENTE DE PLANTA, MéTODO PARA REDUZIR A ATIVIDADE DA LISINA CETOGLUTARATO REDUTASE EM UMA SEMENTE DE PLANTA E PLANTA" A presente invenção se refere a genes quiméricos. Um gene quimérico codifica uma lisina cetogluturato redutase vegetal e um segundo gene quimérico codifica a sintase de ácido dihidrodipicolínico (DHDPS) insensível à lisina que é operavelmente ligada a uma seq³ência de trânsito de cloroplasto vegetal, tudo operacionalmente ligado a seq³ências reguladoras específicas de sementes de plantas, São apresentados os métodos para a sua utilização para produzir maiores níveis de lisina nas sementes das plantas transformadas.
BR9811256-2A 1997-03-27 1998-03-27 Fragmento de ácido nucleico, gene quimérico, célula vegetal, semente de planta, método para reduzir a atividade da lisina cetoglutarato redutase em uma semente de planta e planta BR9811256A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US82462797A 1997-03-27 1997-03-27
PCT/US1998/006051 WO1998042831A2 (en) 1997-03-27 1998-03-27 Chimeric genes and methods for increasing the lysine content of the seeds of plants

Publications (1)

Publication Number Publication Date
BR9811256A true BR9811256A (pt) 2000-07-18

Family

ID=25241893

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9811256-2A BR9811256A (pt) 1997-03-27 1998-03-27 Fragmento de ácido nucleico, gene quimérico, célula vegetal, semente de planta, método para reduzir a atividade da lisina cetoglutarato redutase em uma semente de planta e planta

Country Status (12)

Country Link
EP (1) EP0973880A2 (pt)
JP (1) JP2001502923A (pt)
KR (1) KR20010005645A (pt)
CN (1) CN1253584A (pt)
AU (1) AU747997B2 (pt)
BR (1) BR9811256A (pt)
CA (1) CA2280196C (pt)
HU (1) HUP0002305A3 (pt)
ID (1) ID22486A (pt)
PL (1) PL336042A1 (pt)
TR (1) TR199902349T2 (pt)
WO (1) WO1998042831A2 (pt)

Families Citing this family (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1074629A1 (en) * 1999-07-30 2001-02-07 Coöperatieve Verkoop- en Productievereniging van Aardappelmeel en Derivaten 'AVEBE' B.A. Sink protein
US7157281B2 (en) * 2003-12-11 2007-01-02 Monsanto Technology Llc High lysine maize compositions and event LY038 maize plants
US7855323B2 (en) 2004-02-10 2010-12-21 Monsanto Technology Llc Recombinant DNA for gene suppression
AR047598A1 (es) * 2004-02-10 2006-01-25 Monsanto Technology Llc Semilla de maiz transgenica con mayor contenido de aminoacidos
WO2007041419A1 (en) * 2005-10-03 2007-04-12 Monsanto Technology Llc Transgenic plant seed with increased lysine
CN100412198C (zh) * 2006-04-05 2008-08-20 北京凯拓迪恩生物技术研发中心有限责任公司 一种提高水稻种子中赖氨酸含量的方法及其专用载体
US7964774B2 (en) 2008-05-14 2011-06-21 Monsanto Technology Llc Plants and seeds of spring canola variety SCV384196
MX2011013224A (es) 2009-06-09 2012-06-01 Pioneer Hi Bred Int Promotor de endospermo temprano y metodos de uso.
US8778672B2 (en) 2009-10-26 2014-07-15 Pioneer Hi Bred International Inc Somatic ovule specific promoter and methods of use
CN102051376B (zh) * 2010-01-27 2013-03-20 华中农业大学 基于油菜子叶叶绿体转化的组织培养体系及获得转化植株的方法
US20130167262A1 (en) 2011-12-21 2013-06-27 The Curators Of The University Of Missouri Soybean variety s05-11268
US9204603B2 (en) 2011-12-21 2015-12-08 The Curators Of The University Of Missouri Soybean variety S05-11482
WO2013103371A1 (en) 2012-01-06 2013-07-11 Pioneer Hi-Bred International, Inc. Ovule specific promoter and methods of use
WO2013103365A1 (en) 2012-01-06 2013-07-11 Pioneer Hi-Bred International, Inc. Pollen preferred promoters and methods of use
US8835720B2 (en) 2012-04-26 2014-09-16 Monsanto Technology Llc Plants and seeds of spring canola variety SCV967592
US8878009B2 (en) 2012-04-26 2014-11-04 Monsanto Technology, LLP Plants and seeds of spring canola variety SCV318181
US8859857B2 (en) 2012-04-26 2014-10-14 Monsanto Technology Llc Plants and seeds of spring canola variety SCV259778
WO2014059155A1 (en) 2012-10-11 2014-04-17 Pioneer Hi-Bred International, Inc. Guard cell promoters and uses thereof
US9713332B2 (en) 2013-03-13 2017-07-25 Pioneer Hi-Bred International, Inc. Glyphosate application for weed control in Brassica
EP3744727A1 (en) 2013-03-14 2020-12-02 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
CN105473605A (zh) 2013-03-15 2016-04-06 先锋国际良种公司 Phi-4多肽及其使用方法
MX359026B (es) 2013-08-16 2018-09-12 Pioneer Hi Bred Int Proteinas insecticidas y metodos de uso.
EA031651B1 (ru) 2013-09-13 2019-02-28 Пайонир Хай-Бред Интернэшнл, Инк. Инсектицидные белки и способы их применения
JP2017504329A (ja) * 2014-01-16 2017-02-09 キャリスタ， インコーポレイテッド アミノ酸の増強された産生のための微生物および関連する方法
MX2016010187A (es) 2014-02-07 2017-07-11 Pioneer Hi Bred Int Proteinas insecticidas y metodos para su uso.
US20170218384A1 (en) 2014-08-08 2017-08-03 Pioneer Hi-Bred International, Inc. Ubiquitin promoters and introns and methods of use
US20170247719A1 (en) 2014-09-17 2017-08-31 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
EP3207143B1 (en) 2014-10-16 2023-11-22 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
WO2016099916A1 (en) 2014-12-19 2016-06-23 E. I. Du Pont De Nemours And Company Polylactic acid compositions with accelerated degradation rate and increased heat stability
RU2017144238A (ru) 2015-05-19 2019-06-19 Пайонир Хай-Бред Интернэшнл, Инк. Инсектицидные белки и способы их применения
AU2016278142A1 (en) 2015-06-16 2017-11-30 E. I. Du Pont De Nemours And Company Compositions and methods to control insect pests
CN109475096B (zh) 2015-08-06 2022-08-23 先锋国际良种公司 植物来源的杀昆虫蛋白及其使用方法
CN108513584A (zh) 2015-08-28 2018-09-07 先锋国际良种公司 苍白杆菌介导的植物转化
US20180325119A1 (en) 2015-12-18 2018-11-15 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
US11104911B2 (en) 2015-12-22 2021-08-31 Pioneer Hi-Bred International, Inc. Embryo-preferred Zea mays promoters and methods of use
WO2017192560A1 (en) 2016-05-04 2017-11-09 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
WO2017218207A1 (en) 2016-06-16 2017-12-21 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
UA127388C2 (uk) 2016-06-24 2023-08-09 Піонір Хай-Бред Інтернешнл, Інк. Регуляторний елемент рослини і спосіб його застосування
EP3954202A1 (en) 2016-07-01 2022-02-16 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
WO2018013333A1 (en) 2016-07-12 2018-01-18 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
CN109863167B (zh) 2016-11-01 2022-12-23 先锋国际良种公司 杀昆虫蛋白及其使用方法
BR112020006045A2 (pt) 2017-09-25 2020-10-06 Pioneer Hi-Bred International, Inc. molécula de ácido nucleico, cassete de expressão, vetor, célula vegetal, planta, semente da planta, método para produzir uma planta transgênica, planta transgênica, semente da planta transgênica, método para aprimorar a eficiência de maturação de embrião somático e método para produzir uma planta dicotiledônea transgênica ou uma planta gimnosperma transgênica
BR112020023800A2 (pt) 2018-05-22 2021-02-23 Pioneer Hi-Bred International, Inc. elementos reguladores de planta e métodos de uso dos mesmos
WO2020005933A1 (en) 2018-06-28 2020-01-02 Pioneer Hi-Bred International, Inc. Methods for selecting transformed plants
JP2022512817A (ja) 2018-10-31 2022-02-07 パイオニア ハイ－ブレッド インターナショナル， インコーポレイテッド オクロバクテリウム（Ｏｃｈｒｏｂａｃｔｒｕｍ）媒介植物形質転換のための組成物及び方法
WO2022015619A2 (en) 2020-07-14 2022-01-20 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
CN114317557B (zh) * 2022-01-06 2023-07-07 河南农业大学 玉米ZmRIBA1基因在高赖氨酸玉米育种中的应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5258300A (en) * 1988-06-09 1993-11-02 Molecular Genetics Research And Development Limited Partnership Method of inducing lysine overproduction in plants
EP0640141B1 (en) * 1992-03-19 2003-05-14 E.I. Du Pont De Nemours And Company Nucleic acid fragments and methods for increasing the lysine and threonine content of the seeds of plants
PL314696A1 (en) * 1993-11-30 1996-09-16 Du Pont Chiaeric genes and method of increasing lysine content in soya, maize and rape plant seeds
CN1103819C (zh) * 1994-06-14 2003-03-26 味之素株式会社 α－酮戊二酸脱氢酶基因
US5919617A (en) * 1994-12-21 1999-07-06 Miami University Methods and reagents for detecting fungal pathogens in a biological sample

Also Published As

Publication number Publication date
ID22486A (id) 1999-10-21
WO1998042831A2 (en) 1998-10-01
KR20010005645A (ko) 2001-01-15
AU747997B2 (en) 2002-05-30
CN1253584A (zh) 2000-05-17
CA2280196C (en) 2012-05-15
AU6780198A (en) 1998-10-20
HUP0002305A3 (en) 2002-09-30
WO1998042831A3 (en) 1998-12-10
EP0973880A2 (en) 2000-01-26
PL336042A1 (en) 2000-06-05
HUP0002305A2 (hu) 2000-09-28
TR199902349T2 (xx) 2000-01-21
JP2001502923A (ja) 2001-03-06
CA2280196A1 (en) 1998-10-01

Similar Documents

Publication Publication Date Title
BR9811256A (pt) Fragmento de ácido nucleico, gene quimérico, célula vegetal, semente de planta, método para reduzir a atividade da lisina cetoglutarato redutase em uma semente de planta e planta
Ibrahim et al. Primary, secondary metabolites, photosynthetic capacity and antioxidant activity of the Malaysian Herb Kacip Fatimah (Labisia Pumila Benth) exposed to potassium fertilization under greenhouse conditions
Iijima et al. Characterization of geraniol synthase from the peltate glands of sweet basil
NO20082466L (no) Delta-9-forlengelser og deres anvendelse ved merking av polyumettede fettsyrer
Schmitt et al. Chemical characterization of Allium ursinum L. depending on harvesting time
BR9813968A (pt) Moléculas de ácido nucléico que codificam proteìnas tendo atividade frutosil transferase e processos para produzir inulina de cadeia longa
Keusgen et al. Characterization of some Allium hybrids by aroma precursors, aroma profiles, and alliinase activity
Kopsell et al. Changes in the S-alk (en) yl cysteine sulfoxides and their biosynthetic intermediates during onion storage
Bennett et al. Positional effect on protein and oil content and composition of soybeans
BR9907223A (pt) Métodos e composições para modificação dos nìveis de compostos metabólicos secundários em plantas
Nakamura et al. Measurement of serine acetyltransferase activity in crude plant extracts by a coupled assay system using cysteine synthase
Bernet et al. Changes in polyamine content, arginine and ornithine decarboxylases and transglutaminase activities during light/dark phases (of initial differentiation) in maize calluses and their chloroplasts
Duan et al. Accumulation of salicylic acid-elicited alkaloid compounds in in vitro cultured Pinellia ternata microtubers and expression profiling of genes associated with benzoic acid-derived alkaloid biosynthesis
Satoh et al. Specificity of S-adenosyl-L-methionine in the inactivation and the labeling of 1-aminocyclopropane-1-carboxylate synthase isolated from tomato fruits
Liu et al. Night temperature affects the growth, metabolism, and photosynthetic gene expression in Astragalus membranaceus and Codonopsis lanceolata plug seedlings
Hosseini et al. Functional quality, antioxidant capacity and essential oil percentage in different mint species affected by salinity stress
Kruger et al. Quantitative approaches for analysing fluxes through plant metabolic networks using NMR and stable isotope labelling
Shetty et al. Stimulation of total phenolics, L-DOPA and antioxidant activity through proline-linked pentose phosphate pathway in response to proline and its analogue in germinating fava beans (Vicia faba)
Gonzalez-Reyes et al. Stimulation of onion root elongation by ascorbate and ascorbate free radical in Allium cepa L.
Drapal et al. The chemotype core collection of genus Nicotiana
PT100203A (pt) Sequencia de adn que codifica uma oxalato-oxidase, gene quimerico que incorpora uma tal sequencia, celula vegetal que contem um vector para a transformacao de plantas que compreende um tal gene quimerico e planta transformada proveniente da referida celula
Lynch et al. Variability in the carbon isotope composition of individual amino acids in plant proteins from different sources: 1 Leaves
Santos-Filho et al. Nitrate reductase-and nitric oxide-dependent activation of sinapoylglucose: malate sinapoyltransferase in leaves of Arabidopsis thaliana
Schmitz et al. The genome of Ricinus communis encodes a single glycolate oxidase with different functions in photosynthetic and heterotrophic organs
Downton Further evidence for two modes of carboxyl transfer in plants with C4-photosynthesis

Legal Events

Date Code Title Description
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: INDEFIRO O PEDIDO DE ACORDO COM O(S) ARTIGO(S) 10 DA LPI.

B12B Appeal against refusal [chapter 12.2 patent gazette]
B11D Dismissal acc. art. 38, par 2 of ipl - failure to pay fee after grant in time