WO1998042831A3 - Chimeric genes and methods for increasing the lysine content of the seeds of plants - Google Patents

Chimeric genes and methods for increasing the lysine content of the seeds of plants Download PDF

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Publication number
WO1998042831A3
WO1998042831A3 PCT/US1998/006051 US9806051W WO9842831A3 WO 1998042831 A3 WO1998042831 A3 WO 1998042831A3 US 9806051 W US9806051 W US 9806051W WO 9842831 A3 WO9842831 A3 WO 9842831A3
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WO
WIPO (PCT)
Prior art keywords
seeds
methods
lysine
plants
chimeric genes
Prior art date
Application number
PCT/US1998/006051
Other languages
French (fr)
Other versions
WO1998042831A2 (en
Inventor
Saverio Carl Falco
Raymond Ervin Mcdevitt Iii
Sabine Ursula Epelbaum
Original Assignee
Du Pont
Saverio Carl Falco
Raymond Ervin Mcdevitt Iii
Sabine Ursula Epelbaum
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont, Saverio Carl Falco, Raymond Ervin Mcdevitt Iii, Sabine Ursula Epelbaum filed Critical Du Pont
Priority to JP10543284A priority Critical patent/JP2001502923A/en
Priority to KR1019997008713A priority patent/KR20010005645A/en
Priority to HU0002305A priority patent/HUP0002305A3/en
Priority to BR9811256-2A priority patent/BR9811256A/en
Priority to PL98336042A priority patent/PL336042A1/en
Priority to AU67801/98A priority patent/AU747997B2/en
Priority to CA2280196A priority patent/CA2280196C/en
Priority to EP98913190A priority patent/EP0973880A2/en
Publication of WO1998042831A2 publication Critical patent/WO1998042831A2/en
Publication of WO1998042831A3 publication Critical patent/WO1998042831A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8254—Tryptophan or lysine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Abstract

Chimeric genes are disclosed. One chimeric gene encodes a plant lysine ketoglutarate reductase and a second chimeric gene encodes lysine-insensitive dihydrodipicolinic acid synthase (DHDPS) which is operably linked to a plant chloroplast transit sequence, all operably linked to plant seed-specific regulatory sequences. Methods for their use to produce increased levels of lysine in the seeds of transformed plants are provided.
PCT/US1998/006051 1997-03-27 1998-03-27 Chimeric genes and methods for increasing the lysine content of the seeds of plants WO1998042831A2 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
JP10543284A JP2001502923A (en) 1997-03-27 1998-03-27 Chimeric genes and methods for increasing lysine content in plant seeds
KR1019997008713A KR20010005645A (en) 1997-03-27 1998-03-27 Chimeric Genes and Methods for Increasing the Lysine Content of the Seeds of Plants
HU0002305A HUP0002305A3 (en) 1997-03-27 1998-03-27 Chimeric genes and methods for increasing the lysine content of the seeds of plants
BR9811256-2A BR9811256A (en) 1997-03-27 1998-03-27 Nucleic acid fragment, chimeric gene, plant cell, plant seed, method to reduce lysine ketoglutarate reductase activity in a plant and plant seed
PL98336042A PL336042A1 (en) 1997-03-27 1998-03-27 Chimeric genes and methods of increasing lysine content in seeds of plants
AU67801/98A AU747997B2 (en) 1997-03-27 1998-03-27 Chimeric genes and methods for increasing the lysine content of the seeds of plants
CA2280196A CA2280196C (en) 1997-03-27 1998-03-27 Chimeric genes and methods for increasing the lysine content of the seeds of plants
EP98913190A EP0973880A2 (en) 1997-03-27 1998-03-27 Chimeric genes and methods for increasing the lysine content of the seeds of plants

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US82462797A 1997-03-27 1997-03-27
US08/824,627 1997-03-27

Publications (2)

Publication Number Publication Date
WO1998042831A2 WO1998042831A2 (en) 1998-10-01
WO1998042831A3 true WO1998042831A3 (en) 1998-12-10

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/006051 WO1998042831A2 (en) 1997-03-27 1998-03-27 Chimeric genes and methods for increasing the lysine content of the seeds of plants

Country Status (12)

Country Link
EP (1) EP0973880A2 (en)
JP (1) JP2001502923A (en)
KR (1) KR20010005645A (en)
CN (1) CN1253584A (en)
AU (1) AU747997B2 (en)
BR (1) BR9811256A (en)
CA (1) CA2280196C (en)
HU (1) HUP0002305A3 (en)
ID (1) ID22486A (en)
PL (1) PL336042A1 (en)
TR (1) TR199902349T2 (en)
WO (1) WO1998042831A2 (en)

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US7157281B2 (en) 2003-12-11 2007-01-02 Monsanto Technology Llc High lysine maize compositions and event LY038 maize plants
AR047598A1 (en) * 2004-02-10 2006-01-25 Monsanto Technology Llc TRANSGENIZED CORN SEED WITH GREATER AMINO ACID CONTENT
US7855323B2 (en) 2004-02-10 2010-12-21 Monsanto Technology Llc Recombinant DNA for gene suppression
WO2007041419A1 (en) * 2005-10-03 2007-04-12 Monsanto Technology Llc Transgenic plant seed with increased lysine
CN100412198C (en) * 2006-04-05 2008-08-20 北京凯拓迪恩生物技术研发中心有限责任公司 Method of increasing lysine content in paddy rice seed and special carrier
US7964774B2 (en) 2008-05-14 2011-06-21 Monsanto Technology Llc Plants and seeds of spring canola variety SCV384196
WO2010147825A1 (en) 2009-06-09 2010-12-23 Pioneer Hi-Bred International, Inc. Early endosperm promoter and methods of use
BR112012009044A2 (en) 2009-10-26 2015-09-01 Pioneer Hi Bred Int Isolated nucleic acid molecule, expression cassette, vector, plant cell, plant, transgenic seed, method for expressing a polynucleotide in a plant or plant cell and method for expressing a polynucleotide, preferably in somatic egg tissues of a plant
CN102051376B (en) * 2010-01-27 2013-03-20 华中农业大学 Tissue culture system based on chloroplast transformation in rape cotyledons and method for obtaining transformed plant
US9204603B2 (en) 2011-12-21 2015-12-08 The Curators Of The University Of Missouri Soybean variety S05-11482
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CN105339380A (en) 2013-03-14 2016-02-17 先锋国际良种公司 Compositions and methods to control insect pests
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WO2016099916A1 (en) 2014-12-19 2016-06-23 E. I. Du Pont De Nemours And Company Polylactic acid compositions with accelerated degradation rate and increased heat stability
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Also Published As

Publication number Publication date
CN1253584A (en) 2000-05-17
AU6780198A (en) 1998-10-20
WO1998042831A2 (en) 1998-10-01
HUP0002305A3 (en) 2002-09-30
CA2280196A1 (en) 1998-10-01
PL336042A1 (en) 2000-06-05
HUP0002305A2 (en) 2000-09-28
BR9811256A (en) 2000-07-18
AU747997B2 (en) 2002-05-30
CA2280196C (en) 2012-05-15
ID22486A (en) 1999-10-21
TR199902349T2 (en) 2000-01-21
KR20010005645A (en) 2001-01-15
EP0973880A2 (en) 2000-01-26
JP2001502923A (en) 2001-03-06

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