TR199902349T2 - Chimeric genes, methods to increase lysine content - Google Patents

Chimeric genes, methods to increase lysine content

Info

Publication number
TR199902349T2
TR199902349T2 TR1999/02349T TR9902349T TR199902349T2 TR 199902349 T2 TR199902349 T2 TR 199902349T2 TR 1999/02349 T TR1999/02349 T TR 1999/02349T TR 9902349 T TR9902349 T TR 9902349T TR 199902349 T2 TR199902349 T2 TR 199902349T2
Authority
TR
Turkey
Prior art keywords
methods
chimeric genes
lysine
lysine content
plant
Prior art date
Application number
TR1999/02349T
Other languages
Turkish (tr)
Inventor
Carl Falco Saverio
Ervin Iii Mcdevitt Raymond
Ursula Epelbaum Sabine
Original Assignee
E.I. Du Pont De Nemours And Company
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by E.I. Du Pont De Nemours And Company filed Critical E.I. Du Pont De Nemours And Company
Publication of TR199902349T2 publication Critical patent/TR199902349T2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8254—Tryptophan or lysine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Abstract

Bu bulusta kimerik genler tarif edilmistir. Bir kimerik gen bir bitki lizin ketoglütarat redüktazi kodlar ve bir bitki kloroplast transit serisine baglanabilen lizine hassas olmayan dihidrodipikolinik asit sentazi (DHDPS) kodlar ki bunlarin hepsi islevsel olarak bitki tohumuna spesifik düzenleyici serilere baglanabilirler. Bu bulusta ayrica bunlarin dönüstürülmüs bitkilerin tohumlarindaki lizin seviyesini arttirmak için kullanimlarini saglayan yöntemlerde saglanmistir.Chimeric genes have been described in this invention. A chimeric gene codes for a plant lysine ketoglutarate reductase and codes for lysine-sensitive dihydrodipicolinic acid synthase (DHDPS), which can be linked to a plant chloroplast transit series, all of which can be functionally linked to plant seed specific regulatory series. In the present invention, they are also provided in methods that enable their use to increase the level of lysine in the seeds of transformed plants.

TR1999/02349T 1997-03-27 1998-03-27 Chimeric genes, methods to increase lysine content TR199902349T2 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US82462797A 1997-03-27 1997-03-27

Publications (1)

Publication Number Publication Date
TR199902349T2 true TR199902349T2 (en) 2000-01-21

Family

ID=25241893

Family Applications (1)

Application Number Title Priority Date Filing Date
TR1999/02349T TR199902349T2 (en) 1997-03-27 1998-03-27 Chimeric genes, methods to increase lysine content

Country Status (12)

Country Link
EP (1) EP0973880A2 (en)
JP (1) JP2001502923A (en)
KR (1) KR20010005645A (en)
CN (1) CN1253584A (en)
AU (1) AU747997B2 (en)
BR (1) BR9811256A (en)
CA (1) CA2280196C (en)
HU (1) HUP0002305A3 (en)
ID (1) ID22486A (en)
PL (1) PL336042A1 (en)
TR (1) TR199902349T2 (en)
WO (1) WO1998042831A2 (en)

Families Citing this family (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1074629A1 (en) * 1999-07-30 2001-02-07 Coöperatieve Verkoop- en Productievereniging van Aardappelmeel en Derivaten 'AVEBE' B.A. Sink protein
US7157281B2 (en) 2003-12-11 2007-01-02 Monsanto Technology Llc High lysine maize compositions and event LY038 maize plants
AR047598A1 (en) * 2004-02-10 2006-01-25 Monsanto Technology Llc TRANSGENIZED CORN SEED WITH GREATER AMINO ACID CONTENT
US7855323B2 (en) 2004-02-10 2010-12-21 Monsanto Technology Llc Recombinant DNA for gene suppression
WO2007041419A1 (en) * 2005-10-03 2007-04-12 Monsanto Technology Llc Transgenic plant seed with increased lysine
CN100412198C (en) * 2006-04-05 2008-08-20 北京凯拓迪恩生物技术研发中心有限责任公司 Method of increasing lysine content in paddy rice seed and special carrier
US7964774B2 (en) 2008-05-14 2011-06-21 Monsanto Technology Llc Plants and seeds of spring canola variety SCV384196
WO2010147825A1 (en) 2009-06-09 2010-12-23 Pioneer Hi-Bred International, Inc. Early endosperm promoter and methods of use
BR112012009044A2 (en) 2009-10-26 2015-09-01 Pioneer Hi Bred Int Isolated nucleic acid molecule, expression cassette, vector, plant cell, plant, transgenic seed, method for expressing a polynucleotide in a plant or plant cell and method for expressing a polynucleotide, preferably in somatic egg tissues of a plant
CN102051376B (en) * 2010-01-27 2013-03-20 华中农业大学 Tissue culture system based on chloroplast transformation in rape cotyledons and method for obtaining transformed plant
US9204603B2 (en) 2011-12-21 2015-12-08 The Curators Of The University Of Missouri Soybean variety S05-11482
WO2013096818A1 (en) 2011-12-21 2013-06-27 The Curators Of The University Of Missouri Soybean variety s05-11268
CA2860783A1 (en) 2012-01-06 2013-07-11 Pioneer Hi-Bred International, Inc. Ovule specific promoter and methods of use
US9006515B2 (en) 2012-01-06 2015-04-14 Pioneer Hi Bred International Inc Pollen preferred promoters and methods of use
US8859857B2 (en) 2012-04-26 2014-10-14 Monsanto Technology Llc Plants and seeds of spring canola variety SCV259778
US8878009B2 (en) 2012-04-26 2014-11-04 Monsanto Technology, LLP Plants and seeds of spring canola variety SCV318181
US8835720B2 (en) 2012-04-26 2014-09-16 Monsanto Technology Llc Plants and seeds of spring canola variety SCV967592
CA2887571A1 (en) 2012-10-11 2014-04-17 Pioneer Hi-Bred International, Inc. Guard cell promoters and uses thereof
EA028528B1 (en) 2013-03-13 2017-11-30 Пайонир Хай-Бред Интернэшнл, Инк. Glyphosate application for weed control in brassica
CN105339380A (en) 2013-03-14 2016-02-17 先锋国际良种公司 Compositions and methods to control insect pests
WO2014150914A2 (en) 2013-03-15 2014-09-25 Pioneer Hi-Bred International, Inc. Phi-4 polypeptides and methods for their use
EP3032942B1 (en) 2013-08-16 2020-03-11 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
UA120598C2 (en) 2013-09-13 2020-01-10 Піонір Хай-Бред Інтернешнл, Інк. Insecticidal proteins and methods for their use
JP2017504329A (en) * 2014-01-16 2017-02-09 キャリスタ， インコーポレイテッド Microorganisms and related methods for enhanced production of amino acids
BR112016018287A2 (en) 2014-02-07 2017-10-10 Du Pont insecticide proteins and methods for their use
US20170218384A1 (en) 2014-08-08 2017-08-03 Pioneer Hi-Bred International, Inc. Ubiquitin promoters and introns and methods of use
US20170247719A1 (en) 2014-09-17 2017-08-31 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
WO2016061206A1 (en) 2014-10-16 2016-04-21 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
WO2016099916A1 (en) 2014-12-19 2016-06-23 E. I. Du Pont De Nemours And Company Polylactic acid compositions with accelerated degradation rate and increased heat stability
RU2017144238A (en) 2015-05-19 2019-06-19 Пайонир Хай-Бред Интернэшнл, Инк. INSECTICIDAL PROTEINS AND METHODS OF THEIR APPLICATION
US10647995B2 (en) 2015-06-16 2020-05-12 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
US11198709B2 (en) 2015-08-06 2021-12-14 E. I. Du Pont De Nemours And Company Plant derived insecticidal proteins and methods for their use
CN108513584A (en) 2015-08-28 2018-09-07 先锋国际良种公司 The Plant Transformation that anthropi mediates
CA3002995A1 (en) 2015-12-18 2017-06-22 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
EP3394268B1 (en) 2015-12-22 2023-07-19 Pioneer Hi-Bred International, Inc. Tissue-preferred promoters and methods of use
CA3018384A1 (en) 2016-05-04 2017-11-09 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
CN109312359A (en) 2016-06-16 2019-02-05 先锋国际良种公司 To prevent and treat the composition and method of insect pest
CN116334123A (en) 2016-06-24 2023-06-27 先锋国际良种公司 Plant regulating element and method of use thereof
RU2019102714A (en) 2016-07-01 2020-08-03 Пайонир Хай-Бред Интернэшнл, Инк. INSECTICIDE PROTEINS FROM PLANTS AND METHODS OF THEIR APPLICATION
US20210292778A1 (en) 2016-07-12 2021-09-23 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
WO2018084936A1 (en) 2016-11-01 2018-05-11 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
KR20200056434A (en) 2017-09-25 2020-05-22 파이어니어 하이 부렛드 인터내쇼날 인코포레이팃드 Tissue-preferred promoters and methods of use
BR112020023800A2 (en) 2018-05-22 2021-02-23 Pioneer Hi-Bred International, Inc. plant regulatory elements and methods of using them
US20210277409A1 (en) 2018-06-28 2021-09-09 Pioneer Hi-Bred International, Inc. Methods for selecting transformed plants
EP3874050A1 (en) 2018-10-31 2021-09-08 Pioneer Hi-Bred International, Inc. Compositions and methods for ochrobactrum-mediated plant transformation
EP4182466A2 (en) 2020-07-14 2023-05-24 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
CN114317557B (en) * 2022-01-06 2023-07-07 河南农业大学 Application of corn ZmRIBA1 gene in high-lysine corn breeding

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5258300A (en) * 1988-06-09 1993-11-02 Molecular Genetics Research And Development Limited Partnership Method of inducing lysine overproduction in plants
DE69332974T2 (en) * 1992-03-19 2004-05-19 E.I. Du Pont De Nemours And Co., Wilmington NUCLEIC ACID FRAGMENTS AND METHOD FOR INCREASING THE LYSINE AND THREONINE CONTENT OF THE PLANT SEEDS
PL314696A1 (en) * 1993-11-30 1996-09-16 Du Pont Chiaeric genes and method of increasing lysine content in soya, maize and rape plant seeds
DE69522690T2 (en) * 1994-06-14 2002-06-20 Ajinomoto Kk alpha-ketogultaric acid dehydrogenase gene
US5919617A (en) * 1994-12-21 1999-07-06 Miami University Methods and reagents for detecting fungal pathogens in a biological sample

Also Published As

Publication number Publication date
CN1253584A (en) 2000-05-17
AU6780198A (en) 1998-10-20
WO1998042831A2 (en) 1998-10-01
HUP0002305A3 (en) 2002-09-30
CA2280196A1 (en) 1998-10-01
PL336042A1 (en) 2000-06-05
HUP0002305A2 (en) 2000-09-28
BR9811256A (en) 2000-07-18
WO1998042831A3 (en) 1998-12-10
AU747997B2 (en) 2002-05-30
CA2280196C (en) 2012-05-15
ID22486A (en) 1999-10-21
KR20010005645A (en) 2001-01-15
EP0973880A2 (en) 2000-01-26
JP2001502923A (en) 2001-03-06

Similar Documents

Publication Publication Date Title
TR199902349T2 (en) Chimeric genes, methods to increase lysine content
Perdomo et al. Rubisco and rubisco activase play an important role in the biochemical limitations of photosynthesis in rice, wheat, and maize under high temperature and water deficit
Di Baccio et al. Does glutathione metabolism have a role in the defence of poplar against zinc excess?
Sinha et al. Translocation of metals from fly ash amended soil in the plant of Sesbania cannabina L. Ritz: effect on antioxidants
Arkoun et al. Hydroponics versus field lysimeter studies of urea, ammonium and nitrate uptake by oilseed rape (Brassica napus L.)
Anjum et al. Improving growth and productivity of oleiferous Brassicas under changing environment: significance of nitrogen and sulphur nutrition, and underlying mechanisms
Anjum et al. Sulphur protects mustard (Brassica campestris L.) from cadmium toxicity by improving leaf ascorbate and glutathione: Sulphur protects mustard from cadmium toxicity
Cabrera-Bosquet et al. Photosynthetic capacity of field-grown durum wheat under different N availabilities: A comparative study from leaf to canopy
Barunawati et al. The influence of inorganic nitrogen fertilizer forms on micronutrient retranslocation and accumulation in grains of winter wheat
Ledgard et al. Isotopic fractionation during reduction of nitrate and nitrite by extracts of spinach leaves
Schrader et al. Nitrate uptake, reduction and transport in the whole plant
Bloom et al. Rising atmospheric CO2 concentration inhibits nitrate assimilation in shoots but enhances it in roots of C3 plants
DE69840362D1 (en) REGULATION OF GENE EXPRESSION IN PLANTS
Schiff [28] A green safelight for the study of chloroplast development and other photomorphogenetic phenomena
Mazid et al. Response of crop plants under sulphur stress tolerance: A holistic approach
Lea et al. The use of amino acid analogues in studies on plant metabolism
de Queiroz Barcelos et al. Effects of foliar nickel (Ni) application on mineral nutrition status, urease activity and physiological quality of soybean seeds
GB0121126D0 (en) Systemic non-nodular endosymbiotic nitrogen fixation in plants
Maignan et al. Biostimulant effects of glutacetine® and its derived formulations mixed with N fertilizer on post-heading N uptake and remobilization, seed yield, and grain quality in winter wheat
FI962099A0 (en) Gene encoding carboxypeptidase from Aspergillus Niger
Mustafa et al. Improving crop productivity and nitrogen use efficiency using sulfur and zinc-coated urea: A review
Werth et al. Assimilate partitioning affects 13 C fractionation of recently assimilated carbon in maize
Neelam et al. Evaluation and identification of wheat-Aegilops addition lines controlling high grain iron and zinc concentration and mugineic acid production
PT79191A (en) Selection using opine synthase genes
Goos et al. Response of spring wheat to phosphorus and sulphur starter fertilizers of differing acidification potential