HUP0002305A2 - Chimeric genes and methods for increasing the lysine content of the seeds of plants - Google Patents

Chimeric genes and methods for increasing the lysine content of the seeds of plants

Info

Publication number
HUP0002305A2
HUP0002305A2 HU0002305A HUP0002305A HUP0002305A2 HU P0002305 A2 HUP0002305 A2 HU P0002305A2 HU 0002305 A HU0002305 A HU 0002305A HU P0002305 A HUP0002305 A HU P0002305A HU P0002305 A2 HUP0002305 A2 HU P0002305A2
Authority
HU
Hungary
Prior art keywords
lysine
seeds
plant
plants
chimera
Prior art date
Application number
HU0002305A
Other languages
Hungarian (hu)
Inventor
Sabine Ursula Epelbaum
Saverio Carl Falco
Raymond Ervin Mcdevitt Iii
Original Assignee
E.I.Du Pont De Nemours And Co.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by E.I.Du Pont De Nemours And Co. filed Critical E.I.Du Pont De Nemours And Co.
Publication of HUP0002305A2 publication Critical patent/HUP0002305A2/en
Publication of HUP0002305A3 publication Critical patent/HUP0002305A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8254—Tryptophan or lysine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Developmental Biology & Embryology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pretreatment Of Seeds And Plants (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A találmány tárgyát lizin ketőglűtarát redűktáz enzimet (LKR) egészbenvagy részben kódőló nűkleinsavak képezik. Előnyösen, a találmánytárgyát kiméragének képezik. Egy első kiméragén növényi lizinketőglűtarát redűktáz enzimet (LKR) kódől, egy másődik pedig lizin-inszenzitív dihidrődipikőlinsav szintetázt (DHDPS) kódől növényiklőrőplaszt-tranzitszekvenciával fűnkciőnálisan kapcsőltan; mindegyikgén fűnkciőnálisan növényi magspecifikűs szabályőzó szekvenciákhőz vankapcsőlva. A találmány tárgyát képezik tővábbá, a kiméragénekkeltranszfőrmált növények, azők magvai, valamint eljárásők atranszfőrmált növények magjaiban megnövelt lizinszint elérésére akiméragének alkalmazásával. A találmány szerinti megőldás növényieredetű táplálékők lizintartalmának növelésére alkalmas. ŕThe subject of the invention are nucleic acids encoding the enzyme lysine diglutarate reductase (LKR) in whole or in part. Preferably, the subject of the invention is a chimera gene. A first chimera gene encodes a plant lysine diglutarate reductase enzyme (LKR) and a second one encodes a lysine-insensitive dihydrodipicholic acid synthetase (DHDPS) functionally spliced with a plant plastid transit sequence; each gene is functionally encoded by plant seed-specific regulatory sequences. Further, the subject of the invention is plants transformed with chimera genes, their seeds, as well as methods for achieving an increased level of lysine in the seeds of transformed plants by using chimera genes. The solution according to the invention is suitable for increasing the lysine content of plant-based foods. ŕ

HU0002305A 1997-03-27 1998-03-27 Chimeric genes and methods for increasing the lysine content of the seeds of plants HUP0002305A3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US82462797A 1997-03-27 1997-03-27
PCT/US1998/006051 WO1998042831A2 (en) 1997-03-27 1998-03-27 Chimeric genes and methods for increasing the lysine content of the seeds of plants

Publications (2)

Publication Number Publication Date
HUP0002305A2 true HUP0002305A2 (en) 2000-09-28
HUP0002305A3 HUP0002305A3 (en) 2002-09-30

Family

ID=25241893

Family Applications (1)

Application Number Title Priority Date Filing Date
HU0002305A HUP0002305A3 (en) 1997-03-27 1998-03-27 Chimeric genes and methods for increasing the lysine content of the seeds of plants

Country Status (12)

Country Link
EP (1) EP0973880A2 (en)
JP (1) JP2001502923A (en)
KR (1) KR20010005645A (en)
CN (1) CN1253584A (en)
AU (1) AU747997B2 (en)
BR (1) BR9811256A (en)
CA (1) CA2280196C (en)
HU (1) HUP0002305A3 (en)
ID (1) ID22486A (en)
PL (1) PL336042A1 (en)
TR (1) TR199902349T2 (en)
WO (1) WO1998042831A2 (en)

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US7157281B2 (en) 2003-12-11 2007-01-02 Monsanto Technology Llc High lysine maize compositions and event LY038 maize plants
AR047598A1 (en) * 2004-02-10 2006-01-25 Monsanto Technology Llc TRANSGENIZED CORN SEED WITH GREATER AMINO ACID CONTENT
US7855323B2 (en) 2004-02-10 2010-12-21 Monsanto Technology Llc Recombinant DNA for gene suppression
BRPI0616848A8 (en) * 2005-10-03 2017-09-19 Monsanto Technology Llc TRANSGENIC PLANT SEED WITH HIGHER LYSINE CONTENT
CN100412198C (en) * 2006-04-05 2008-08-20 北京凯拓迪恩生物技术研发中心有限责任公司 Method of increasing lysine content in paddy rice seed and special carrier
US7964774B2 (en) 2008-05-14 2011-06-21 Monsanto Technology Llc Plants and seeds of spring canola variety SCV384196
US8466342B2 (en) 2009-06-09 2013-06-18 Pioneer Hi Bred International Inc Early endosperm promoter and methods of use
CA2775146A1 (en) 2009-10-26 2011-05-12 Pioneer Hi-Bred International, Inc. Somatic ovule specific promoter and methods of use
CN102051376B (en) * 2010-01-27 2013-03-20 华中农业大学 Tissue culture system based on chloroplast transformation in rape cotyledons and method for obtaining transformed plant
US9204603B2 (en) 2011-12-21 2015-12-08 The Curators Of The University Of Missouri Soybean variety S05-11482
WO2013096818A1 (en) 2011-12-21 2013-06-27 The Curators Of The University Of Missouri Soybean variety s05-11268
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US8878009B2 (en) 2012-04-26 2014-11-04 Monsanto Technology, LLP Plants and seeds of spring canola variety SCV318181
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US8835720B2 (en) 2012-04-26 2014-09-16 Monsanto Technology Llc Plants and seeds of spring canola variety SCV967592
WO2014059155A1 (en) 2012-10-11 2014-04-17 Pioneer Hi-Bred International, Inc. Guard cell promoters and uses thereof
CA2905743C (en) 2013-03-13 2021-09-28 Pioneer Hi-Bred International, Inc. Glyphosate application for weed control in brassica
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EP2971000A4 (en) 2013-03-15 2016-11-23 Pioneer Hi Bred Int Phi-4 polypeptides and methods for their use
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US11236347B2 (en) 2015-08-28 2022-02-01 Pioneer Hi-Bred International, Inc. Ochrobactrum-mediated transformation of plants
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Also Published As

Publication number Publication date
PL336042A1 (en) 2000-06-05
KR20010005645A (en) 2001-01-15
CA2280196C (en) 2012-05-15
ID22486A (en) 1999-10-21
JP2001502923A (en) 2001-03-06
CA2280196A1 (en) 1998-10-01
BR9811256A (en) 2000-07-18
AU747997B2 (en) 2002-05-30
WO1998042831A3 (en) 1998-12-10
TR199902349T2 (en) 2000-01-21
CN1253584A (en) 2000-05-17
EP0973880A2 (en) 2000-01-26
HUP0002305A3 (en) 2002-09-30
WO1998042831A2 (en) 1998-10-01
AU6780198A (en) 1998-10-20

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