HUP0002305A2 - Chimeric genes and methods for increasing the lysine content of the seeds of plants - Google Patents
Chimeric genes and methods for increasing the lysine content of the seeds of plantsInfo
- Publication number
- HUP0002305A2 HUP0002305A2 HU0002305A HUP0002305A HUP0002305A2 HU P0002305 A2 HUP0002305 A2 HU P0002305A2 HU 0002305 A HU0002305 A HU 0002305A HU P0002305 A HUP0002305 A HU P0002305A HU P0002305 A2 HUP0002305 A2 HU P0002305A2
- Authority
- HU
- Hungary
- Prior art keywords
- lysine
- seeds
- plant
- plants
- chimera
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 6
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 title abstract 5
- 239000004472 Lysine Substances 0.000 title abstract 5
- 102000004316 Oxidoreductases Human genes 0.000 abstract 2
- 108090000854 Oxidoreductases Proteins 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 102000003960 Ligases Human genes 0.000 abstract 1
- 108090000364 Ligases Proteins 0.000 abstract 1
- 239000002253 acid Substances 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 235000021135 plant-based food Nutrition 0.000 abstract 1
- 210000002706 plastid Anatomy 0.000 abstract 1
- 230000001105 regulatory effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
- C12N15/823—Reproductive tissue-specific promoters
- C12N15/8234—Seed-specific, e.g. embryo, endosperm
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
- C12N15/8254—Tryptophan or lysine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Nutrition Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Developmental Biology & Embryology (AREA)
- Pregnancy & Childbirth (AREA)
- Reproductive Health (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
A találmány tárgyát lizin ketőglűtarát redűktáz enzimet (LKR) egészbenvagy részben kódőló nűkleinsavak képezik. Előnyösen, a találmánytárgyát kiméragének képezik. Egy első kiméragén növényi lizinketőglűtarát redűktáz enzimet (LKR) kódől, egy másődik pedig lizin-inszenzitív dihidrődipikőlinsav szintetázt (DHDPS) kódől növényiklőrőplaszt-tranzitszekvenciával fűnkciőnálisan kapcsőltan; mindegyikgén fűnkciőnálisan növényi magspecifikűs szabályőzó szekvenciákhőz vankapcsőlva. A találmány tárgyát képezik tővábbá, a kiméragénekkeltranszfőrmált növények, azők magvai, valamint eljárásők atranszfőrmált növények magjaiban megnövelt lizinszint elérésére akiméragének alkalmazásával. A találmány szerinti megőldás növényieredetű táplálékők lizintartalmának növelésére alkalmas. ŕThe subject of the invention are nucleic acids encoding the enzyme lysine diglutarate reductase (LKR) in whole or in part. Preferably, the subject of the invention is a chimera gene. A first chimera gene encodes a plant lysine diglutarate reductase enzyme (LKR) and a second one encodes a lysine-insensitive dihydrodipicholic acid synthetase (DHDPS) functionally spliced with a plant plastid transit sequence; each gene is functionally encoded by plant seed-specific regulatory sequences. Further, the subject of the invention is plants transformed with chimera genes, their seeds, as well as methods for achieving an increased level of lysine in the seeds of transformed plants by using chimera genes. The solution according to the invention is suitable for increasing the lysine content of plant-based foods. ŕ
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US82462797A | 1997-03-27 | 1997-03-27 | |
PCT/US1998/006051 WO1998042831A2 (en) | 1997-03-27 | 1998-03-27 | Chimeric genes and methods for increasing the lysine content of the seeds of plants |
Publications (2)
Publication Number | Publication Date |
---|---|
HUP0002305A2 true HUP0002305A2 (en) | 2000-09-28 |
HUP0002305A3 HUP0002305A3 (en) | 2002-09-30 |
Family
ID=25241893
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
HU0002305A HUP0002305A3 (en) | 1997-03-27 | 1998-03-27 | Chimeric genes and methods for increasing the lysine content of the seeds of plants |
Country Status (12)
Country | Link |
---|---|
EP (1) | EP0973880A2 (en) |
JP (1) | JP2001502923A (en) |
KR (1) | KR20010005645A (en) |
CN (1) | CN1253584A (en) |
AU (1) | AU747997B2 (en) |
BR (1) | BR9811256A (en) |
CA (1) | CA2280196C (en) |
HU (1) | HUP0002305A3 (en) |
ID (1) | ID22486A (en) |
PL (1) | PL336042A1 (en) |
TR (1) | TR199902349T2 (en) |
WO (1) | WO1998042831A2 (en) |
Families Citing this family (49)
Publication number | Priority date | Publication date | Assignee | Title |
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EP1074629A1 (en) * | 1999-07-30 | 2001-02-07 | Coöperatieve Verkoop- en Productievereniging van Aardappelmeel en Derivaten 'AVEBE' B.A. | Sink protein |
US7157281B2 (en) * | 2003-12-11 | 2007-01-02 | Monsanto Technology Llc | High lysine maize compositions and event LY038 maize plants |
US7855323B2 (en) | 2004-02-10 | 2010-12-21 | Monsanto Technology Llc | Recombinant DNA for gene suppression |
AR047598A1 (en) * | 2004-02-10 | 2006-01-25 | Monsanto Technology Llc | TRANSGENIZED CORN SEED WITH GREATER AMINO ACID CONTENT |
US20070079396A1 (en) * | 2005-10-03 | 2007-04-05 | Malvar Thomas M | Transgenic plant seed with increased lysine |
CN100412198C (en) * | 2006-04-05 | 2008-08-20 | 北京凯拓迪恩生物技术研发中心有限责任公司 | Method of increasing lysine content in paddy rice seed and special carrier |
US7964774B2 (en) | 2008-05-14 | 2011-06-21 | Monsanto Technology Llc | Plants and seeds of spring canola variety SCV384196 |
CA2765034A1 (en) | 2009-06-09 | 2010-12-23 | Pioneer Hi-Bred International, Inc. | Early endosperm promoter and methods of use |
EP2494056A1 (en) | 2009-10-26 | 2012-09-05 | Pioneer Hi-Bred International Inc. | Somatic ovule specific promoter and methods of use |
CN102051376B (en) * | 2010-01-27 | 2013-03-20 | 华中农业大学 | Tissue culture system based on chloroplast transformation in rape cotyledons and method for obtaining transformed plant |
US20130167262A1 (en) | 2011-12-21 | 2013-06-27 | The Curators Of The University Of Missouri | Soybean variety s05-11268 |
US9204603B2 (en) | 2011-12-21 | 2015-12-08 | The Curators Of The University Of Missouri | Soybean variety S05-11482 |
BR112014016791A2 (en) | 2012-01-06 | 2019-09-24 | Pioneer Hi Bred Int | isolated nucleic acid molecule, expression cassette, vector, plant cell, plant, transgenic seed, method for expression of a polynucleotide in a plant or plant cell, method for expression of a polynucleotide, preferably in egg tissues of a plant |
US9006515B2 (en) | 2012-01-06 | 2015-04-14 | Pioneer Hi Bred International Inc | Pollen preferred promoters and methods of use |
US8835720B2 (en) | 2012-04-26 | 2014-09-16 | Monsanto Technology Llc | Plants and seeds of spring canola variety SCV967592 |
US8859857B2 (en) | 2012-04-26 | 2014-10-14 | Monsanto Technology Llc | Plants and seeds of spring canola variety SCV259778 |
US8878009B2 (en) | 2012-04-26 | 2014-11-04 | Monsanto Technology, LLP | Plants and seeds of spring canola variety SCV318181 |
WO2014059155A1 (en) | 2012-10-11 | 2014-04-17 | Pioneer Hi-Bred International, Inc. | Guard cell promoters and uses thereof |
AU2014241045B2 (en) | 2013-03-13 | 2017-08-31 | Pioneer Hi-Bred International, Inc. | Glyphosate application for weed control in brassica |
CN105339380A (en) | 2013-03-14 | 2016-02-17 | 先锋国际良种公司 | Compositions and methods to control insect pests |
US10023877B2 (en) | 2013-03-15 | 2018-07-17 | Pioneer Hi-Bred International, Inc. | PHI-4 polypeptides and methods for their use |
EA030896B1 (en) | 2013-08-16 | 2018-10-31 | Пайонир Хай-Бред Интернэшнл, Инк. | Insecticidal proteins and methods for their use |
BR122021005579B1 (en) | 2013-09-13 | 2022-11-29 | Pioneer Hi-Bred International, Inc | DNA CONSTRUCTION, METHOD FOR OBTAINING A TRANSGENIC PLANT, FUSION PROTEIN, METHOD FOR CONTROLLING AN INSECT PEST POPULATION, METHOD FOR INHIBITING THE GROWTH OR KILLING AN INSECT PEST |
JP2017504329A (en) * | 2014-01-16 | 2017-02-09 | キャリスタ, インコーポレイテッド | Microorganisms and related methods for enhanced production of amino acids |
CA2939156A1 (en) | 2014-02-07 | 2015-08-13 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
US20170218384A1 (en) | 2014-08-08 | 2017-08-03 | Pioneer Hi-Bred International, Inc. | Ubiquitin promoters and introns and methods of use |
US20170247719A1 (en) | 2014-09-17 | 2017-08-31 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
BR112017007932A2 (en) | 2014-10-16 | 2018-01-23 | Du Pont | insecticide proteins and methods for their use |
WO2016099916A1 (en) | 2014-12-19 | 2016-06-23 | E. I. Du Pont De Nemours And Company | Polylactic acid compositions with accelerated degradation rate and increased heat stability |
CN116333064A (en) | 2015-05-19 | 2023-06-27 | 先锋国际良种公司 | Insecticidal proteins and methods of use thereof |
CA2986265A1 (en) | 2015-06-16 | 2016-12-22 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
WO2017023486A1 (en) | 2015-08-06 | 2017-02-09 | Pioneer Hi-Bred International, Inc. | Plant derived insecticidal proteins and methods for their use |
CN108513584A (en) | 2015-08-28 | 2018-09-07 | 先锋国际良种公司 | The Plant Transformation that anthropi mediates |
CN108575091A (en) | 2015-12-18 | 2018-09-25 | 先锋国际良种公司 | insecticidal proteins and methods of use thereof |
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CA3022858A1 (en) | 2016-06-16 | 2017-12-21 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
BR112018076816A2 (en) | 2016-06-24 | 2019-09-03 | Pioneer Hi Bred Int | hybrid regulatory element, hybrid promoter, DNA construct, expression cassette, host cell, transgenic plant, method for creating a hybrid regulatory element, and method for targeted expression of a polynucleotide sequence in a plant or plant cell |
EP3954202A1 (en) | 2016-07-01 | 2022-02-16 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins from plants and methods for their use |
WO2018013333A1 (en) | 2016-07-12 | 2018-01-18 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
EP3535285B1 (en) | 2016-11-01 | 2022-04-06 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
WO2019060383A1 (en) | 2017-09-25 | 2019-03-28 | Pioneer Hi-Bred, International, Inc. | Tissue-preferred promoters and methods of use |
CN111867377B (en) | 2018-03-14 | 2023-05-23 | 先锋国际良种公司 | Insecticidal proteins from plants and methods of use thereof |
CN115850420A (en) | 2018-03-14 | 2023-03-28 | 先锋国际良种公司 | Insecticidal proteins from plants and methods of use thereof |
WO2019226508A1 (en) | 2018-05-22 | 2019-11-28 | Pioneer Hi-Bred International, Inc. | Plant regulatory elements and methods of use thereof |
WO2020005933A1 (en) | 2018-06-28 | 2020-01-02 | Pioneer Hi-Bred International, Inc. | Methods for selecting transformed plants |
JP2022512817A (en) | 2018-10-31 | 2022-02-07 | パイオニア ハイ-ブレッド インターナショナル, インコーポレイテッド | Compositions and Methods for Ochrobactrum-mediated Plant Transformation |
WO2022015619A2 (en) | 2020-07-14 | 2022-01-20 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
CN114317557B (en) * | 2022-01-06 | 2023-07-07 | 河南农业大学 | Application of corn ZmRIBA1 gene in high-lysine corn breeding |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5258300A (en) * | 1988-06-09 | 1993-11-02 | Molecular Genetics Research And Development Limited Partnership | Method of inducing lysine overproduction in plants |
DE69332974T2 (en) * | 1992-03-19 | 2004-05-19 | E.I. Du Pont De Nemours And Co., Wilmington | NUCLEIC ACID FRAGMENTS AND METHOD FOR INCREASING THE LYSINE AND THREONINE CONTENT OF THE PLANT SEEDS |
JPH09511124A (en) * | 1993-11-30 | 1997-11-11 | イー・アイ・デユポン・ドウ・ヌムール・アンド・カンパニー | Chimeric genes and corn, soybean and rapeseed plants |
CN1272437C (en) * | 1994-06-14 | 2006-08-30 | 味之素株式会社 | Alph-ketoglutaric acid dehydrase gene |
US5919617A (en) * | 1994-12-21 | 1999-07-06 | Miami University | Methods and reagents for detecting fungal pathogens in a biological sample |
-
1998
- 1998-03-27 CN CN98803732A patent/CN1253584A/en active Pending
- 1998-03-27 TR TR1999/02349T patent/TR199902349T2/en unknown
- 1998-03-27 PL PL98336042A patent/PL336042A1/en unknown
- 1998-03-27 BR BR9811256-2A patent/BR9811256A/en not_active Application Discontinuation
- 1998-03-27 WO PCT/US1998/006051 patent/WO1998042831A2/en not_active Application Discontinuation
- 1998-03-27 AU AU67801/98A patent/AU747997B2/en not_active Ceased
- 1998-03-27 HU HU0002305A patent/HUP0002305A3/en unknown
- 1998-03-27 EP EP98913190A patent/EP0973880A2/en not_active Withdrawn
- 1998-03-27 KR KR1019997008713A patent/KR20010005645A/en not_active Application Discontinuation
- 1998-03-27 CA CA2280196A patent/CA2280196C/en not_active Expired - Fee Related
- 1998-03-27 JP JP10543284A patent/JP2001502923A/en not_active Ceased
- 1998-03-27 ID IDW991102A patent/ID22486A/en unknown
Also Published As
Publication number | Publication date |
---|---|
WO1998042831A3 (en) | 1998-12-10 |
KR20010005645A (en) | 2001-01-15 |
AU6780198A (en) | 1998-10-20 |
CA2280196C (en) | 2012-05-15 |
HUP0002305A3 (en) | 2002-09-30 |
BR9811256A (en) | 2000-07-18 |
CN1253584A (en) | 2000-05-17 |
TR199902349T2 (en) | 2000-01-21 |
PL336042A1 (en) | 2000-06-05 |
JP2001502923A (en) | 2001-03-06 |
ID22486A (en) | 1999-10-21 |
AU747997B2 (en) | 2002-05-30 |
EP0973880A2 (en) | 2000-01-26 |
CA2280196A1 (en) | 1998-10-01 |
WO1998042831A2 (en) | 1998-10-01 |
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