HUP0200286A2 - Nucleic acid sequences encoding isoflavone synthase - Google Patents

Nucleic acid sequences encoding isoflavone synthase

Info

Publication number
HUP0200286A2
HUP0200286A2 HU0200286A HUP0200286A HUP0200286A2 HU P0200286 A2 HUP0200286 A2 HU P0200286A2 HU 0200286 A HU0200286 A HU 0200286A HU P0200286 A HUP0200286 A HU P0200286A HU P0200286 A2 HUP0200286 A2 HU P0200286A2
Authority
HU
Hungary
Prior art keywords
nucleic acid
acid sequences
sequences encoding
isoflavone
isoflavone synthase
Prior art date
Application number
HU0200286A
Other languages
Hungarian (hu)
Inventor
Gary M. Fader
Woosuk Jung
Brian Mcgonigle
Joan T. Odell
Xiaodan Yu
Original Assignee
E.I. Du Pont De Nemours And Co.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by E.I. Du Pont De Nemours And Co. filed Critical E.I. Du Pont De Nemours And Co.
Publication of HUP0200286A2 publication Critical patent/HUP0200286A2/en
Publication of HUP0200286A3 publication Critical patent/HUP0200286A3/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0071Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/825Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02Oxygen as only ring hetero atoms
    • C12P17/06Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A találmány tárgyát izoflavon-szintetázt kódoló, izolált nukleinsav-szekvenciák képezik. A találmány tárgyát képezik továbbá az enzimbármely lényeges részét, szensz vagy antiszensz orientációban kódolókiméraszekvencia-konstrukciók, ahol a kiméraszekvencia expresszálásaaz enzim megváltozott mértékű termelését eredményezi a transzformáltgazdasejtben, valamint a nukleinsav-szekvenciák által kódoltpolipeptidek. A találmány szerinti megoldás alkalmas növényekizoflavon-háztartásának befolyásolására és izoflavon-tartalmánakmegváltoztatására. ÓThe invention relates to isolated nucleic acid sequences encoding isoflavone synthetase. The invention also includes chimeric sequence constructs encoding any essential part of the enzyme in sense or antisense orientation, where expression of the chimeric sequence results in altered production of the enzyme in the transformed host cell, as well as polypeptides encoded by the nucleic acid sequences. The solution according to the invention is suitable for influencing the isoflavone balance of plants and changing their isoflavone content. HE

HU0200286A 1999-01-27 2000-01-26 Nucleic acid sequences encoding isoflavone synthase HUP0200286A3 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US11776999P 1999-01-27 1999-01-27
US14478399P 1999-07-20 1999-07-20
US15609499P 1999-09-24 1999-09-24

Publications (2)

Publication Number Publication Date
HUP0200286A2 true HUP0200286A2 (en) 2002-05-29
HUP0200286A3 HUP0200286A3 (en) 2003-12-29

Family

ID=27382035

Family Applications (1)

Application Number Title Priority Date Filing Date
HU0200286A HUP0200286A3 (en) 1999-01-27 2000-01-26 Nucleic acid sequences encoding isoflavone synthase

Country Status (5)

Country Link
EP (1) EP1147199A1 (en)
AU (1) AU2858500A (en)
CA (1) CA2353306A1 (en)
HU (1) HUP0200286A3 (en)
WO (1) WO2000044909A1 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4517100B2 (en) 1999-02-04 2010-08-04 学校法人日本大学 Polynucleotide encoding 2-hydroxyisoflavanone synthase
WO2000053771A1 (en) * 1999-03-08 2000-09-14 The Samuel Roberts Noble Foundation, Inc. Genetic manipulation of isoflavonoids
US7208659B2 (en) 2001-05-02 2007-04-24 Conopco Inc. Process for increasing the flavonoid content of a plant and plants obtainable thereby
EP1401260A4 (en) * 2001-06-13 2005-04-06 Du Pont A method for altering the isoflavonoid profile in the plant parts of an isoflavonoid-producing plant
AUPR811301A0 (en) 2001-10-05 2001-10-25 Agresearch Limited Manipulation of flavonoid biosynthesis
EP1478757A2 (en) 2002-02-28 2004-11-24 Unilever Plc Production of dadzein in transgenic plants
US7189895B2 (en) 2002-06-13 2007-03-13 E. I. Du Pont De Nemours And Company Methods to increase the isoflavonoid levels in plants and plants producing increased levels of isoflavonoids
US7750211B2 (en) 2002-09-10 2010-07-06 The Samuel Roberts Noble Foundation Methods and compositions for production of flavonoid and isoflavonoid nutraceuticals
WO2005103258A1 (en) * 2004-04-20 2005-11-03 Grain Biotech Australia Pty Ltd A plant, its use as a nutraceutical and the identification thereof
WO2007056347A1 (en) 2005-11-07 2007-05-18 E. I. Du Pont De Nemours And Company Compositions with increased phytosterol levels obtained from plants with decreased triterpene saponin levels
US7816507B2 (en) 2006-02-09 2010-10-19 The Samuel Roberts Noble Foundation Methods and compositions for modifying plant biosynthetic pathways

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6121512A (en) * 1997-10-10 2000-09-19 North Carolina State University Cytochrome P-450 constructs and method of producing herbicide-resistant transgenic plants

Also Published As

Publication number Publication date
AU2858500A (en) 2000-08-18
WO2000044909A1 (en) 2000-08-03
EP1147199A1 (en) 2001-10-24
HUP0200286A3 (en) 2003-12-29
CA2353306A1 (en) 2000-08-03

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