CA2280196C - Genes chimeres et procede permettant d'accroitre la teneur en lysine dans les graines de vegetaux - Google Patents
Genes chimeres et procede permettant d'accroitre la teneur en lysine dans les graines de vegetaux Download PDFInfo
- Publication number
- CA2280196C CA2280196C CA2280196A CA2280196A CA2280196C CA 2280196 C CA2280196 C CA 2280196C CA 2280196 A CA2280196 A CA 2280196A CA 2280196 A CA2280196 A CA 2280196A CA 2280196 C CA2280196 C CA 2280196C
- Authority
- CA
- Canada
- Prior art keywords
- lysine
- gene
- seq
- seeds
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
- C12N15/823—Reproductive tissue-specific promoters
- C12N15/8234—Seed-specific, e.g. embryo, endosperm
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
- C12N15/8254—Tryptophan or lysine
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Nutrition Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Developmental Biology & Embryology (AREA)
- Pregnancy & Childbirth (AREA)
- Reproductive Health (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
L'invention porte sur des gènes chimères, un gène chimère codant une cétoglutarate réductase de la lysine et un second gène chimère codant une synthase d'acide dihydrodipicolinique insensible à la lysine et qui est lié de manière fonctionnelle à une séquence de transit de chloroplaste de végétaux, ces gènes étant liés de manière fonctionnelle à des séquences régulatrices spécifiques des graines de végétaux. L'invention porte également sur les procédés d'utilisation de ces gènes en vue de produire des taux supérieurs de lysine dans les graines de végétaux transformés.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US82462797A | 1997-03-27 | 1997-03-27 | |
US08/824,627 | 1997-03-27 | ||
PCT/US1998/006051 WO1998042831A2 (fr) | 1997-03-27 | 1998-03-27 | Genes chimeres et procede permettant d'accroitre la teneur en lysine dans les graines de vegetaux |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2280196A1 CA2280196A1 (fr) | 1998-10-01 |
CA2280196C true CA2280196C (fr) | 2012-05-15 |
Family
ID=25241893
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2280196A Expired - Fee Related CA2280196C (fr) | 1997-03-27 | 1998-03-27 | Genes chimeres et procede permettant d'accroitre la teneur en lysine dans les graines de vegetaux |
Country Status (12)
Country | Link |
---|---|
EP (1) | EP0973880A2 (fr) |
JP (1) | JP2001502923A (fr) |
KR (1) | KR20010005645A (fr) |
CN (1) | CN1253584A (fr) |
AU (1) | AU747997B2 (fr) |
BR (1) | BR9811256A (fr) |
CA (1) | CA2280196C (fr) |
HU (1) | HUP0002305A3 (fr) |
ID (1) | ID22486A (fr) |
PL (1) | PL336042A1 (fr) |
TR (1) | TR199902349T2 (fr) |
WO (1) | WO1998042831A2 (fr) |
Families Citing this family (47)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1074629A1 (fr) * | 1999-07-30 | 2001-02-07 | Coöperatieve Verkoop- en Productievereniging van Aardappelmeel en Derivaten 'AVEBE' B.A. | Protéines pièges |
US7157281B2 (en) | 2003-12-11 | 2007-01-02 | Monsanto Technology Llc | High lysine maize compositions and event LY038 maize plants |
US7855323B2 (en) | 2004-02-10 | 2010-12-21 | Monsanto Technology Llc | Recombinant DNA for gene suppression |
AR047598A1 (es) * | 2004-02-10 | 2006-01-25 | Monsanto Technology Llc | Semilla de maiz transgenica con mayor contenido de aminoacidos |
UA102367C2 (ru) * | 2005-10-03 | 2013-07-10 | Монсанто Текнолоджи Ллс | Семя трансгенной кукурузы с повышенным содержанием лизина |
CN100412198C (zh) * | 2006-04-05 | 2008-08-20 | 北京凯拓迪恩生物技术研发中心有限责任公司 | 一种提高水稻种子中赖氨酸含量的方法及其专用载体 |
US7964774B2 (en) | 2008-05-14 | 2011-06-21 | Monsanto Technology Llc | Plants and seeds of spring canola variety SCV384196 |
WO2010147825A1 (fr) | 2009-06-09 | 2010-12-23 | Pioneer Hi-Bred International, Inc. | Promoteur d'endosperme précoce et procédés d'utilisation |
WO2011056544A1 (fr) | 2009-10-26 | 2011-05-12 | Pioneer Hi-Bred International, Inc. | Promoteur somatique spécifique à un ovule et procédés d'utilisation |
CN102051376B (zh) * | 2010-01-27 | 2013-03-20 | 华中农业大学 | 基于油菜子叶叶绿体转化的组织培养体系及获得转化植株的方法 |
WO2013096810A1 (fr) | 2011-12-21 | 2013-06-27 | The Curators Of The University Of Missouri | Variété de soja s05-11482 |
WO2013096818A1 (fr) | 2011-12-21 | 2013-06-27 | The Curators Of The University Of Missouri | Variété de soja s05-11268 |
US20130180008A1 (en) | 2012-01-06 | 2013-07-11 | Pioneer Hi Bred International Inc | Ovule Specific Promoter and Methods of Use |
WO2013103365A1 (fr) | 2012-01-06 | 2013-07-11 | Pioneer Hi-Bred International, Inc. | Promoteurs de pollen préférés et procédés d'utilisation |
US8835720B2 (en) | 2012-04-26 | 2014-09-16 | Monsanto Technology Llc | Plants and seeds of spring canola variety SCV967592 |
US8859857B2 (en) | 2012-04-26 | 2014-10-14 | Monsanto Technology Llc | Plants and seeds of spring canola variety SCV259778 |
US8878009B2 (en) | 2012-04-26 | 2014-11-04 | Monsanto Technology, LLP | Plants and seeds of spring canola variety SCV318181 |
US20150259696A1 (en) | 2012-10-11 | 2015-09-17 | Shane E. Abbitt | Guard cell promoters and uses thereof |
US9713332B2 (en) | 2013-03-13 | 2017-07-25 | Pioneer Hi-Bred International, Inc. | Glyphosate application for weed control in Brassica |
BR112015023285B1 (pt) | 2013-03-14 | 2022-03-03 | Pioneer Hi-Bred International, Inc. | Cassete de expressão, célula hospedeira bacteriana e método para controlar uma praga de planta do tipo coleóptero |
CA2901316A1 (fr) | 2013-03-15 | 2014-09-25 | Pioneer Hi-Bred International, Inc. | Polypeptides phi-4 et leurs procedes d'utilisation |
CN106232820A (zh) | 2013-08-16 | 2016-12-14 | 先锋国际良种公司 | 杀昆虫蛋白及其使用方法 |
CN105705007A (zh) | 2013-09-13 | 2016-06-22 | 先锋国际良种公司 | 杀昆虫蛋白及其使用方法 |
JP2017504329A (ja) * | 2014-01-16 | 2017-02-09 | キャリスタ, インコーポレイテッド | アミノ酸の増強された産生のための微生物および関連する方法 |
CN106536545A (zh) | 2014-02-07 | 2017-03-22 | 先锋国际良种公司 | 杀昆虫蛋白及其使用方法 |
WO2016022516A1 (fr) | 2014-08-08 | 2016-02-11 | Pioneer Hi-Bred International, Inc. | Promoteurs d'ubiquitine et introns, et procédés d'utilisation |
CA2961733A1 (fr) | 2014-09-17 | 2016-03-24 | Pioneer Hi-Bred International, Inc. | Compositions et procedes de lutte contre des insectes ravageurs |
EP3207143B1 (fr) | 2014-10-16 | 2023-11-22 | Pioneer Hi-Bred International, Inc. | Protéines insecticides et leurs procédés d'utilisation |
US20170359965A1 (en) | 2014-12-19 | 2017-12-21 | E I Du Pont De Nemours And Company | Polylactic acid compositions with accelerated degradation rate and increased heat stability |
RU2017144238A (ru) | 2015-05-19 | 2019-06-19 | Пайонир Хай-Бред Интернэшнл, Инк. | Инсектицидные белки и способы их применения |
MX2017016119A (es) | 2015-06-16 | 2018-03-07 | Pioneer Hi Bred Int | Composiciones y metodos para controlar plagas de insectos. |
US11198709B2 (en) | 2015-08-06 | 2021-12-14 | E. I. Du Pont De Nemours And Company | Plant derived insecticidal proteins and methods for their use |
WO2017040343A1 (fr) | 2015-08-28 | 2017-03-09 | Pioneer Hi-Bred International, Inc. | Transformation de plantes médiée par ochrobactrum |
CA3002995A1 (fr) | 2015-12-18 | 2017-06-22 | Pioneer Hi-Bred International, Inc. | Proteines insecticides et leurs procedes d'utilisation |
US11104911B2 (en) | 2015-12-22 | 2021-08-31 | Pioneer Hi-Bred International, Inc. | Embryo-preferred Zea mays promoters and methods of use |
EP3960863A1 (fr) | 2016-05-04 | 2022-03-02 | Pioneer Hi-Bred International, Inc. | Protéines insecticides et leurs procédés d'utilisation |
CA3022858A1 (fr) | 2016-06-16 | 2017-12-21 | Pioneer Hi-Bred International, Inc. | Compositions et procedes de lutte contre des insectes nuisibles |
BR112018076816A2 (pt) | 2016-06-24 | 2019-09-03 | Pioneer Hi Bred Int | elemento regulador híbrido, promotor híbrido, construto de dna, cassete de expressão, célula hospedeira, planta transgênica, método para criar um elemento regulador híbrido e método para expressão direcionada de uma sequência de polinucleotídeos em uma planta ou célula vegetal |
CA3026113A1 (fr) | 2016-07-01 | 2018-01-04 | Pioneer Hi-Bred International, Inc. | Proteines insecticides issues de plantes et procedes pour leur utilisation |
US20210292778A1 (en) | 2016-07-12 | 2021-09-23 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
US11021716B2 (en) | 2016-11-01 | 2021-06-01 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
JP2020536515A (ja) | 2017-09-25 | 2020-12-17 | パイオニア ハイ−ブレッド インターナショナル, インコーポレイテッド | 組織優先的プロモーター及びその使用方法 |
BR112020023800A2 (pt) | 2018-05-22 | 2021-02-23 | Pioneer Hi-Bred International, Inc. | elementos reguladores de planta e métodos de uso dos mesmos |
US20210277409A1 (en) | 2018-06-28 | 2021-09-09 | Pioneer Hi-Bred International, Inc. | Methods for selecting transformed plants |
JP2022512817A (ja) | 2018-10-31 | 2022-02-07 | パイオニア ハイ-ブレッド インターナショナル, インコーポレイテッド | オクロバクテリウム(Ochrobactrum)媒介植物形質転換のための組成物及び方法 |
CN115867564A (zh) | 2020-07-14 | 2023-03-28 | 先锋国际良种公司 | 杀昆虫蛋白及其使用方法 |
CN114317557B (zh) * | 2022-01-06 | 2023-07-07 | 河南农业大学 | 玉米ZmRIBA1基因在高赖氨酸玉米育种中的应用 |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5258300A (en) * | 1988-06-09 | 1993-11-02 | Molecular Genetics Research And Development Limited Partnership | Method of inducing lysine overproduction in plants |
EP1394257B1 (fr) * | 1992-03-19 | 2007-08-08 | E.I. Dupont De Nemours And Company | fragments d'acide nucléique et procédé d' augmentation de la teneur en lysine et en thréonine de graines de plantes |
KR960706564A (ko) * | 1993-11-30 | 1996-12-09 | 미리암 디. 메코너헤이 | 키메릭 유전자 및 옥수수, 대두 및 평지 식물의 종자중 리신 함량을 증가시키는 방법(Chimeric Genes and Methods for Increasing the Lysine Content of the Seeds of Corn, Soybean and Rapeseed Plants) |
CN1272437C (zh) * | 1994-06-14 | 2006-08-30 | 味之素株式会社 | α-酮戊二酸脱氢酶基因以及赖氨酸的生产方法 |
US5919617A (en) * | 1994-12-21 | 1999-07-06 | Miami University | Methods and reagents for detecting fungal pathogens in a biological sample |
-
1998
- 1998-03-27 CN CN98803732A patent/CN1253584A/zh active Pending
- 1998-03-27 BR BR9811256-2A patent/BR9811256A/pt not_active Application Discontinuation
- 1998-03-27 ID IDW991102A patent/ID22486A/id unknown
- 1998-03-27 WO PCT/US1998/006051 patent/WO1998042831A2/fr not_active Application Discontinuation
- 1998-03-27 HU HU0002305A patent/HUP0002305A3/hu unknown
- 1998-03-27 EP EP98913190A patent/EP0973880A2/fr not_active Withdrawn
- 1998-03-27 AU AU67801/98A patent/AU747997B2/en not_active Ceased
- 1998-03-27 JP JP10543284A patent/JP2001502923A/ja not_active Ceased
- 1998-03-27 KR KR1019997008713A patent/KR20010005645A/ko not_active Application Discontinuation
- 1998-03-27 TR TR1999/02349T patent/TR199902349T2/xx unknown
- 1998-03-27 CA CA2280196A patent/CA2280196C/fr not_active Expired - Fee Related
- 1998-03-27 PL PL98336042A patent/PL336042A1/xx unknown
Also Published As
Publication number | Publication date |
---|---|
CA2280196A1 (fr) | 1998-10-01 |
AU747997B2 (en) | 2002-05-30 |
CN1253584A (zh) | 2000-05-17 |
HUP0002305A3 (en) | 2002-09-30 |
JP2001502923A (ja) | 2001-03-06 |
EP0973880A2 (fr) | 2000-01-26 |
BR9811256A (pt) | 2000-07-18 |
KR20010005645A (ko) | 2001-01-15 |
WO1998042831A3 (fr) | 1998-12-10 |
AU6780198A (en) | 1998-10-20 |
TR199902349T2 (xx) | 2000-01-21 |
WO1998042831A2 (fr) | 1998-10-01 |
ID22486A (id) | 1999-10-21 |
PL336042A1 (en) | 2000-06-05 |
HUP0002305A2 (hu) | 2000-09-28 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKLA | Lapsed |
Effective date: 20170327 |