CA2241267A1 - Ameliorations apportees a des methodes pour transformer des souches de phaffia, souches de phaffia ainsi obtenues et adn de recombinaison obtenu selon lesdites methodes - Google Patents
Ameliorations apportees a des methodes pour transformer des souches de phaffia, souches de phaffia ainsi obtenues et adn de recombinaison obtenu selon lesdites methodesInfo
- Publication number
- CA2241267A1 CA2241267A1 CA002241267A CA2241267A CA2241267A1 CA 2241267 A1 CA2241267 A1 CA 2241267A1 CA 002241267 A CA002241267 A CA 002241267A CA 2241267 A CA2241267 A CA 2241267A CA 2241267 A1 CA2241267 A1 CA 2241267A1
- Authority
- CA
- Canada
- Prior art keywords
- seqidno
- recombinant dna
- phaffia
- sequence
- gene
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
- C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
- C12Y102/01012—Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) (1.2.1.12)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Saccharide Compounds (AREA)
Abstract
La présente invention concerne un ADN de recombinaison comprenant un promoteur de transcription, une séquence en aval à exprimer, liée de façon opérationnelle avec ce dernier, le promoteur de transcription comprenant une région située en amont du cadre de lecture ouvert d'un gène de Phaffia fortement exprimé, de préférence un gène intervenant dans un processus glycolytique, ou encore mieux, le gène codant la glycéraldéhyde-3-phosphate déshydrogénase. D'autres ADN de recombinaison préférés, selon l'invention, contiennent des promoteurs de gènes codant une protéine ribosomique, ou encore mieux, le promoteur de transcription comprenant une région située en amont du cadre de lecture ouvert codant une protéine représentée par l'une des séquences d'acides aminés décrites dans l'une des séquences portant le numéro d'identification 24 à 50. Selon un autre aspect de l'invention, on décrit une séquence d'ADN isolé codant une enzyme impliquée dans le processus de biosynthèse de caroténoïde de Phaffia rhodozyma, ladite enzyme présentant de préférence une activité sélectionnée parmi l'activité de l'isopenténylpyrophosphate isomérase, l'activité de la géranylgéranylpyrophosphate synthase, l'activité de la phytoène synthase, l'activité de la phytoène désaturase et l'activité de la lycopène cyclase, ou encore mieux, celles qui codent un enzyme présentant une séquence d'acides aminés choisie dans l'une des séquences portant le numéro d'identification 13, 15, 17, 19, 21, 23. L'invention concerne également des vecteurs, des organismes hôtes transformés, des méthodes pour produire ces protéines et/ou caroténoïdes, par exemple l'astaxanthine, ainsi que des méthodes pour isoler des promoteurs fortement exprimés à partir du Phaffia.
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP95203620 | 1995-12-22 | ||
EP96200943.7 | 1996-04-11 | ||
EP95203620.0 | 1996-04-11 | ||
EP96200943 | 1996-04-11 | ||
PCT/EP1996/005887 WO1997023633A1 (fr) | 1995-12-22 | 1996-12-23 | Ameliorations apportees a des methodes pour transformer des souches de phaffia, souches de phaffia ainsi obtenues et adn de recombinaison obtenu selon lesdites methodes |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2241267A1 true CA2241267A1 (fr) | 1997-07-03 |
CA2241267C CA2241267C (fr) | 2012-12-04 |
Family
ID=26139955
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2241267A Expired - Lifetime CA2241267C (fr) | 1995-12-22 | 1996-12-23 | Ameliorations apportees a des methodes pour transformer des souches de phaffia, souches de phaffia ainsi obtenues et adn de recombinaison obtenu selon lesdites methodes |
Country Status (9)
Country | Link |
---|---|
US (2) | US6329141B1 (fr) |
EP (2) | EP0870042B1 (fr) |
JP (1) | JP2000507087A (fr) |
AT (1) | ATE275632T1 (fr) |
AU (1) | AU725340B2 (fr) |
CA (1) | CA2241267C (fr) |
DE (1) | DE69633343T2 (fr) |
ES (1) | ES2229288T3 (fr) |
WO (1) | WO1997023633A1 (fr) |
Families Citing this family (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP3151371B2 (ja) | 1995-03-10 | 2001-04-03 | 麒麟麦酒株式会社 | カロチノイド生産量の増量に有用なdna鎖 |
AU725340B2 (en) * | 1995-12-22 | 2000-10-12 | Gist-Brocades B.V. | Improved methods for transforming Phaffia strains, transformed Phaffia strains so obtained and recombinant DNA in said methods |
US6265186B1 (en) | 1997-04-11 | 2001-07-24 | Dsm N.V. | Yeast cells comprising at least two copies of a desired gene integrated into the chromosomal genome at more than one non-ribosomal RNA encoding domain, particularly with Kluyveromyces |
EP0955363A3 (fr) * | 1998-05-06 | 2004-01-28 | F. Hoffmann-La Roche Ag | Séquences d'ADN codant pour des enzymes impliqués dans la production des isoprénoides |
TWI250210B (en) | 1998-05-06 | 2006-03-01 | Dsm Ip Assets Bv | An isolated DNA sequence coding for an enzyme involved in the mevalonate pathway or the pathway from isopentenyl pyrophosphate to farnesyl pyrophosphate |
MX219130B (es) * | 1998-07-06 | 2004-02-10 | Dcv Inc Haciendo Negocios Como | Metodo para producir vitamina. |
US6531303B1 (en) | 1998-07-06 | 2003-03-11 | Arkion Life Sciences Llc | Method of producing geranylgeraniol |
KR100316741B1 (ko) * | 1998-10-31 | 2002-09-04 | 한국생명공학연구원 | 파피아로도지마형질전환용벡터및그형질전환방법 |
DE60005871T2 (de) * | 1999-03-09 | 2004-05-06 | Dsm Ip Assets B.V. | Astaxanthin Synthetase |
AUPQ262599A0 (en) * | 1999-09-02 | 1999-09-23 | University Of Queensland, The | Gene expression |
KR100371264B1 (ko) * | 2000-01-25 | 2003-02-05 | 양문식 | 무라인 그래뉼로사이트-매크로파지 콜로니 자극 인자를포함하는 재조합 벡터와 그것을 발현하는 재조합아스퍼질러스 나이거 |
DE60115757T2 (de) | 2000-05-24 | 2006-07-27 | Dsm Ip Assets B.V. | Verfahren zur Herstellung von Astaxanthin |
US6660507B2 (en) | 2000-09-01 | 2003-12-09 | E. I. Du Pont De Nemours And Company | Genes involved in isoprenoid compound production |
US6818424B2 (en) * | 2000-09-01 | 2004-11-16 | E. I. Du Pont De Nemours And Company | Production of cyclic terpenoids |
WO2002044357A2 (fr) | 2000-11-30 | 2002-06-06 | Millennium Pharmaceuticals, Inc. | Procedes d'utilisation de 18903 dans le traitement de la douleur et des troubles lies a la douleur |
WO2005123915A1 (fr) * | 2004-06-21 | 2005-12-29 | Novozymes A/S | Copies multiples d'au moins deux cadres de lecture ouverte maintenus stables dans la meme orientation |
EP2371967B1 (fr) | 2005-03-18 | 2015-06-03 | DSM IP Assets B.V. | Production de caroténoïdes dans des levures oléagineuses et des champignons |
EP2078092A2 (fr) | 2006-09-28 | 2009-07-15 | Microbia, Inc. | Production de caroténoïdes dans des levures et des champignons oléagineux |
RU2014121383A (ru) | 2011-11-28 | 2016-01-27 | Дайити Санкио Компани, Лимитед | Происходящий из гена человека промотор |
JP2015226469A (ja) * | 2012-09-24 | 2015-12-17 | 株式会社カネカ | 新規dna、およびそれを利用したカロテノイドの製造方法 |
JP2017515512A (ja) * | 2014-05-16 | 2017-06-15 | アカデミア シニカAcademia Sinica | アスタキサンチンを生産するための組換えポリヌクレオチド配列及びその使用 |
WO2020162959A1 (fr) | 2019-02-07 | 2020-08-13 | The General Hospital Corporation | Caroténoïdes pour le traitement ou la prévention de la nausée |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA1341116C (fr) | 1983-02-22 | 2000-10-17 | Rae Lyn Burke | Systemes d'expression de la levure avec des vecteurs ayant des promoteurs de gapdh ou pyk, et synthese de proteine etrangere |
ATE102250T1 (de) | 1984-05-11 | 1994-03-15 | Chiron Corp | Erhoehte hefetranskription unter verwendung einer hybridkonstruktion der promotorregion. |
CA2047000A1 (fr) * | 1990-07-20 | 1992-01-21 | John W. Chapman | Cellules de levure produisant de l'astaxanthine |
GB2254536B (en) | 1991-04-08 | 1994-10-05 | Petrella Ltd | A food product |
DK115191D0 (da) * | 1991-06-14 | 1991-06-14 | Ingrid Stampe Villadsen | Fremgangsmaade til frembringelse af carotenoidproducerende, isaer astaxanthinproducerende, mikroorganismer, mikroorganismer opnaaet ved fremgangsmaaden og fremgangsmaade til fremstilling af carotenoidholdige mikroorganismeceller eller -celledele eller oprenset carotenoid |
JPH05168465A (ja) * | 1991-12-25 | 1993-07-02 | Lion Corp | ファフィア・ロドチーマの突然変異株およびその培養によるカロチノイド類の製造方法 |
NZ248628A (en) * | 1992-09-11 | 1996-02-27 | Gist Brocades Nv | Transformed phaffia (yeast) strains and methods and vectors used |
JP3151371B2 (ja) * | 1995-03-10 | 2001-04-03 | 麒麟麦酒株式会社 | カロチノイド生産量の増量に有用なdna鎖 |
AU725340B2 (en) * | 1995-12-22 | 2000-10-12 | Gist-Brocades B.V. | Improved methods for transforming Phaffia strains, transformed Phaffia strains so obtained and recombinant DNA in said methods |
-
1996
- 1996-12-23 AU AU13087/97A patent/AU725340B2/en not_active Ceased
- 1996-12-23 WO PCT/EP1996/005887 patent/WO1997023633A1/fr active IP Right Grant
- 1996-12-23 EP EP96944694A patent/EP0870042B1/fr not_active Expired - Lifetime
- 1996-12-23 AT AT96944694T patent/ATE275632T1/de not_active IP Right Cessation
- 1996-12-23 EP EP04075855A patent/EP1640456A3/fr not_active Withdrawn
- 1996-12-23 DE DE69633343T patent/DE69633343T2/de not_active Expired - Lifetime
- 1996-12-23 ES ES96944694T patent/ES2229288T3/es not_active Expired - Lifetime
- 1996-12-23 CA CA2241267A patent/CA2241267C/fr not_active Expired - Lifetime
- 1996-12-23 US US09/091,725 patent/US6329141B1/en not_active Expired - Lifetime
- 1996-12-23 JP JP09523340A patent/JP2000507087A/ja active Pending
-
2001
- 2001-10-30 US US10/001,192 patent/US7205123B2/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
WO1997023633A1 (fr) | 1997-07-03 |
DE69633343D1 (de) | 2004-10-14 |
US6329141B1 (en) | 2001-12-11 |
US20040091958A1 (en) | 2004-05-13 |
ES2229288T3 (es) | 2005-04-16 |
US7205123B2 (en) | 2007-04-17 |
DE69633343T2 (de) | 2005-09-22 |
AU1308797A (en) | 1997-07-17 |
EP0870042A1 (fr) | 1998-10-14 |
EP1640456A2 (fr) | 2006-03-29 |
CA2241267C (fr) | 2012-12-04 |
JP2000507087A (ja) | 2000-06-13 |
AU725340B2 (en) | 2000-10-12 |
EP1640456A3 (fr) | 2008-10-01 |
EP0870042B1 (fr) | 2004-09-08 |
ATE275632T1 (de) | 2004-09-15 |
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Legal Events
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EEER | Examination request | ||
MKEX | Expiry |
Effective date: 20161223 |
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MKEX | Expiry |
Effective date: 20161223 |