CA2219891C - Methods and reagents for combined pcr amplification and hybridization probing assay - Google Patents
Methods and reagents for combined pcr amplification and hybridization probing assay Download PDFInfo
- Publication number
- CA2219891C CA2219891C CA002219891A CA2219891A CA2219891C CA 2219891 C CA2219891 C CA 2219891C CA 002219891 A CA002219891 A CA 002219891A CA 2219891 A CA2219891 A CA 2219891A CA 2219891 C CA2219891 C CA 2219891C
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- Prior art keywords
- oligonucleotide
- probe
- oligonucleotide probe
- fluorescer
- pcr
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- Expired - Lifetime
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- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000001018 xanthene dye Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/911—Microorganisms using fungi
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- Life Sciences & Earth Sciences (AREA)
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- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US43550995A | 1995-05-05 | 1995-05-05 | |
| US08/435,509 | 1995-05-05 | ||
| PCT/US1996/004693 WO1996034983A1 (en) | 1995-05-05 | 1996-04-05 | Methods and reagents for combined pcr amplification and hybridization probing assay |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2219891A1 CA2219891A1 (en) | 1996-11-07 |
| CA2219891C true CA2219891C (en) | 2002-01-29 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002219891A Expired - Lifetime CA2219891C (en) | 1995-05-05 | 1996-04-05 | Methods and reagents for combined pcr amplification and hybridization probing assay |
Country Status (8)
| Country | Link |
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| US (8) | US5691146A (enExample) |
| EP (1) | EP0826066B1 (enExample) |
| JP (3) | JP4040087B2 (enExample) |
| AT (1) | ATE196322T1 (enExample) |
| AU (1) | AU693023B2 (enExample) |
| CA (1) | CA2219891C (enExample) |
| DE (1) | DE69610304T2 (enExample) |
| WO (1) | WO1996034983A1 (enExample) |
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-
1996
- 1996-04-05 DE DE69610304T patent/DE69610304T2/de not_active Expired - Lifetime
- 1996-04-05 WO PCT/US1996/004693 patent/WO1996034983A1/en not_active Ceased
- 1996-04-05 CA CA002219891A patent/CA2219891C/en not_active Expired - Lifetime
- 1996-04-05 EP EP96911592A patent/EP0826066B1/en not_active Expired - Lifetime
- 1996-04-05 JP JP53330996A patent/JP4040087B2/ja not_active Expired - Fee Related
- 1996-04-05 AU AU54428/96A patent/AU693023B2/en not_active Expired
- 1996-04-05 AT AT96911592T patent/ATE196322T1/de not_active IP Right Cessation
- 1996-09-11 US US08/710,075 patent/US5691146A/en not_active Expired - Lifetime
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1997
- 1997-04-03 US US08/826,538 patent/US6485903B1/en not_active Expired - Fee Related
- 1997-05-19 US US08/859,472 patent/US6395518B1/en not_active Expired - Lifetime
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2002
- 2002-11-12 US US10/293,236 patent/US20030215826A1/en not_active Abandoned
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2003
- 2003-04-02 JP JP2003099758A patent/JP4040519B2/ja not_active Expired - Fee Related
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2004
- 2004-09-06 JP JP2004259091A patent/JP2005110666A/ja not_active Ceased
- 2004-09-20 US US10/946,458 patent/US7241596B2/en not_active Expired - Fee Related
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2007
- 2007-07-09 US US11/775,151 patent/US7413708B2/en not_active Expired - Fee Related
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2008
- 2008-08-18 US US12/193,667 patent/US7847076B2/en not_active Expired - Fee Related
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2010
- 2010-10-27 US US12/913,714 patent/US20110275071A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| AU693023B2 (en) | 1998-06-18 |
| US6485903B1 (en) | 2002-11-26 |
| JP4040087B2 (ja) | 2008-01-30 |
| US20030215826A1 (en) | 2003-11-20 |
| US20050227247A1 (en) | 2005-10-13 |
| WO1996034983A1 (en) | 1996-11-07 |
| EP0826066B1 (en) | 2000-09-13 |
| US20110275071A1 (en) | 2011-11-10 |
| US6395518B1 (en) | 2002-05-28 |
| DE69610304T2 (de) | 2001-04-05 |
| AU5442896A (en) | 1996-11-21 |
| US7413708B2 (en) | 2008-08-19 |
| US7241596B2 (en) | 2007-07-10 |
| JP2005110666A (ja) | 2005-04-28 |
| JP2004000185A (ja) | 2004-01-08 |
| DE69610304D1 (de) | 2000-10-19 |
| CA2219891A1 (en) | 1996-11-07 |
| US5691146A (en) | 1997-11-25 |
| JPH11504219A (ja) | 1999-04-20 |
| JP4040519B2 (ja) | 2008-01-30 |
| US20080050742A1 (en) | 2008-02-28 |
| US7847076B2 (en) | 2010-12-07 |
| EP0826066A1 (en) | 1998-03-04 |
| US20090258351A1 (en) | 2009-10-15 |
| ATE196322T1 (de) | 2000-09-15 |
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