CA1320162C - Methode de stabilisation de l'expression de la proteine heterologue, et vecteurs servant a cette fin - Google Patents

Methode de stabilisation de l'expression de la proteine heterologue, et vecteurs servant a cette fin

Info

Publication number
CA1320162C
CA1320162C CA000607908A CA607908A CA1320162C CA 1320162 C CA1320162 C CA 1320162C CA 000607908 A CA000607908 A CA 000607908A CA 607908 A CA607908 A CA 607908A CA 1320162 C CA1320162 C CA 1320162C
Authority
CA
Canada
Prior art keywords
cat
protein
gene
expression
gene sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA000607908A
Other languages
English (en)
Inventor
Sandra Hilliker
R. Tyler White
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Scios LLC
Original Assignee
California Biotechnology Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by California Biotechnology Inc filed Critical California Biotechnology Inc
Application granted granted Critical
Publication of CA1320162C publication Critical patent/CA1320162C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12N9/1033—Chloramphenicol O-acetyltransferase (2.3.1.28)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4711—Alzheimer's disease; Amyloid plaque core protein
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/58—Atrial natriuretic factor complex; Atriopeptin; Atrial natriuretic peptide [ANP]; Cardionatrin; Cardiodilatin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/605—Glucagons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/785—Alveolar surfactant peptides; Pulmonary surfactant peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biomedical Technology (AREA)
  • Toxicology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Endocrinology (AREA)
  • Pulmonology (AREA)
  • Cardiology (AREA)
  • Neurology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA000607908A 1988-08-11 1989-08-09 Methode de stabilisation de l'expression de la proteine heterologue, et vecteurs servant a cette fin Expired - Fee Related CA1320162C (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US23122488A 1988-08-11 1988-08-11
US231,224 1988-08-11

Publications (1)

Publication Number Publication Date
CA1320162C true CA1320162C (fr) 1993-07-13

Family

ID=22868276

Family Applications (1)

Application Number Title Priority Date Filing Date
CA000607908A Expired - Fee Related CA1320162C (fr) 1988-08-11 1989-08-09 Methode de stabilisation de l'expression de la proteine heterologue, et vecteurs servant a cette fin

Country Status (6)

Country Link
EP (1) EP0428615A4 (fr)
JP (1) JPH04501204A (fr)
AU (1) AU4216689A (fr)
CA (1) CA1320162C (fr)
IL (1) IL91272A0 (fr)
WO (1) WO1990001540A1 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW327194B (en) * 1992-05-01 1998-02-21 American Cyanamid Co Novel amyloid precursor proteins and methods of using same
NL9201173A (nl) * 1992-07-01 1994-02-01 Leuven K U Res & Dev Vector voor positieve selectie van recombinanten.
DK144093D0 (fr) * 1993-12-23 1993-12-23 Novo Nordisk As
US6031075A (en) * 1996-02-05 2000-02-29 Children's Hospital Medical Center Mature alveolar SP-B and a process for producing the same
JP4741076B2 (ja) * 1998-07-10 2011-08-03 サイオス インコーポレイテッド ８より上または５未満のｐＩを有するペプチドを産生するための方法
AT410444B (de) * 2001-03-02 2003-04-25 Oesterr Forsch Seibersdorf Verfahren zur detektion von nukleinsäuremolekülen
EP2261250B1 (fr) 2001-12-21 2015-07-01 Human Genome Sciences, Inc. Protéines de fusion d'albumine et GCSF
HUE027902T2 (en) 2004-02-09 2016-11-28 Human Genome Sciences Inc Corp Service Company Albumin fusion proteins
AU2009324637B2 (en) 2008-12-10 2016-09-08 Paka Pulmonary Pharmaceuticals, Inc. Methods and compositions for delivery of medicaments to the lungs
CN117343200B (zh) * 2023-12-04 2024-01-30 北京质肽生物医药科技有限公司 一种包含淀粉样核心多肽的融合蛋白、制备方法及其应用

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8314362D0 (en) * 1983-05-24 1983-06-29 Celltech Ltd Polypeptide and protein products
US4933280A (en) * 1984-12-11 1990-06-12 California Biotechnology Inc. Recombinant DNA sequence encoding Alveolar Surfactant Protein
GR861037B (en) * 1985-04-22 1986-07-16 Genentech Inc Novel human tissue plasminogen activator mutants
DE3523701A1 (de) * 1985-07-03 1987-01-08 Bayer Ag Verfahren zur herstellung von proteinen und polypeptiden
GB8518753D0 (en) * 1985-07-24 1985-08-29 Glaxo Group Ltd Microbiological products
EP0290516A4 (fr) * 1986-10-24 1989-11-14 Jeffrey A Whitsett Proteines associees a des agents tensioactifs hydrophobes pulmonaires.
ES2120401T3 (es) * 1986-11-17 1998-11-01 Scios Inc Proteina recombinante amiloide relacionados con la enfermedad de alzheimer.
EP0383837B1 (fr) * 1987-11-04 1998-02-04 Byk Gulden Lomberg Chemische Fabrik GmbH Proteines a effet tensio-actif alveolaire

Also Published As

Publication number Publication date
EP0428615A1 (fr) 1991-05-29
IL91272A0 (en) 1990-03-19
WO1990001540A1 (fr) 1990-02-22
AU4216689A (en) 1990-03-05
JPH04501204A (ja) 1992-03-05
EP0428615A4 (en) 1991-11-13

Similar Documents

Publication Publication Date Title
US6500645B1 (en) N-terminally extended proteins expressed in yeast
Kaster et al. Analysis of a bacterial hygromycin B resistance gene by transcriptional and translational fusions and by DNA sequencing
JP3730255B2 (ja) イーストにおいて発現されたｎ末端に伸長した蛋白質
EP0542863B1 (fr) Expression de polypeptides recombines avec purification amelioree
KR100227167B1 (ko) 인체혈청알부민의 n-말단 단편을 함유하는 융합단백질
US20070010439A1 (en) Production of human parathyroid hormone from microorganisms
CA1320162C (fr) Methode de stabilisation de l'expression de la proteine heterologue, et vecteurs servant a cette fin
JPH0759193B2 (ja) 外来性蛋白質発現用クロ−ニングベクタ−
JP4624495B2 (ja) ヒト・インスリンの生成
JP2970811B2 (ja) 原核生物細胞中にポリペプチドを調製するための発現システム
JP3305781B2 (ja) 新規なるｄｎａ分子及び宿主
EP0797671B1 (fr) Procede de production de proteines extracellulaires dans des bacteries
US6962796B1 (en) Production of human parathyroid hormone from microorganisms
EP0946735B1 (fr) Proteines a extension d'extremite n-terminal exprimees dans la levure
HU216335B (hu) Eljárás peptidek előállítására
PT87211B (pt) Processo para a preparacao de polipeptideos por manipulacao genetica
EP0225860B1 (fr) Méthode pour transmettre des produits de gènes à un milieu de croissance de bactéries gram-négatives
JP4180112B2 (ja) 酵母細胞におけるｎ末端を伸長されたタンパクの発現のためのベクター
US5378613A (en) Method for increased expression of low molecular weight recombinant polypeptides
JPH05500615A (ja) ヒルジン及び新規のヒルジンの組換え製造方法
KR900001014B1 (ko) 소 성장 호르몬 유도체 발현 벡터의 제조방법
Ishizaki et al. Production of recombinant human glucagon in the form of a fusion protein in Escherichia coli; recovery of glucagon by sequence-specific digestion
JP2769541B2 (ja) 平衡型構成誘導性転写系
KR900001015B1 (ko) 재조합체 dna 발현 벡터의 제조방법
PT607080E (pt) Nova sequencia pro que permite a secrecao de proteinas heterologas a partir de uma celula de levedura

Legal Events

Date Code Title Description
MKLA Lapsed