CA1258611A - Methode de sequencage de l'adn - Google Patents

Methode de sequencage de l'adn

Info

Publication number
CA1258611A
CA1258611A CA000472103A CA472103A CA1258611A CA 1258611 A CA1258611 A CA 1258611A CA 000472103 A CA000472103 A CA 000472103A CA 472103 A CA472103 A CA 472103A CA 1258611 A CA1258611 A CA 1258611A
Authority
CA
Canada
Prior art keywords
dna
sequencing
gel
labeled
reactions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
CA000472103A
Other languages
English (en)
Inventor
Lloyd M. Smith
Tim J. Hunkapiller
Leroy E. Hood
Michael W. Hunkapiller
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
California Institute of Technology CalTech
Original Assignee
California Institute of Technology CalTech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by California Institute of Technology CalTech filed Critical California Institute of Technology CalTech
Application granted granted Critical
Publication of CA1258611A publication Critical patent/CA1258611A/fr
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
CA000472103A 1984-01-16 1985-01-15 Methode de sequencage de l'adn Expired CA1258611A (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US57097384A 1984-01-16 1984-01-16
US570,973 1984-01-16
US68901385A 1985-01-02 1985-01-02
US689,013 1985-01-02

Publications (1)

Publication Number Publication Date
CA1258611A true CA1258611A (fr) 1989-08-22

Family

ID=27075453

Family Applications (1)

Application Number Title Priority Date Filing Date
CA000472103A Expired CA1258611A (fr) 1984-01-16 1985-01-15 Methode de sequencage de l'adn

Country Status (5)

Country Link
CA (1) CA1258611A (fr)
DE (1) DE3501306C2 (fr)
FR (1) FR2558262B1 (fr)
GB (1) GB2155176B (fr)
SE (1) SE456348B (fr)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4865968A (en) * 1985-04-01 1989-09-12 The Salk Institute For Biological Studies DNA sequencing
US4855225A (en) * 1986-02-07 1989-08-08 Applied Biosystems, Inc. Method of detecting electrophoretically separated oligonucleotides
US4811218A (en) * 1986-06-02 1989-03-07 Applied Biosystems, Inc. Real time scanning electrophoresis apparatus for DNA sequencing
DE3618605A1 (de) * 1986-06-03 1987-12-10 Europ Lab Molekularbiolog Vorrichtung zur detektion von zur photonenemission anregbaren stoffen
DE3621631A1 (de) * 1986-06-27 1988-01-14 Hirschmann Geraetebau Gmbh & C Geraet zur abbildung eines objektes auf einem elektrooptischen wandler
US5242796A (en) * 1986-07-02 1993-09-07 E. I. Du Pont De Nemours And Company Method, system and reagents for DNA sequencing
CA1340806C (fr) * 1986-07-02 1999-11-02 James Merrill Prober Methode, systeme et reactifs pour le sequencage de l'adn
JPH0799353B2 (ja) * 1987-03-31 1995-10-25 株式会社島津製作所 塩基配列決定装置
DE3752148T2 (de) * 1987-06-09 1998-09-17 Perkin Elmer Corp Echtzeitabtastvorrichtung in einem Elektrophoreseapparat zur DNS-Sequenzbestimmung
US4833332A (en) * 1987-06-12 1989-05-23 E. I. Du Pont De Nemours And Company Scanning fluorescent detection system
US5102785A (en) * 1987-09-28 1992-04-07 E. I. Du Pont De Nemours And Company Method of gene mapping
JPH01224657A (ja) * 1988-03-04 1989-09-07 Hitachi Ltd 核酸の塩基配列決定装置
EP0341928A1 (fr) * 1988-05-10 1989-11-15 AMERSHAM INTERNATIONAL plc Capteurs à résonance de plasmons de surface
SE8802573D0 (sv) * 1988-07-08 1988-07-08 Wallac Oy Multi-label time-resolved fluorescence analysis of nucleic acid sequences using lanthanide chelates
FR2636739B1 (fr) * 1988-09-20 1993-02-12 Commissariat Energie Atomique Procede et installation d'identification des bases d'un adn
FR2636738B1 (fr) * 1988-09-20 1992-12-11 Commissariat Energie Atomique Procede et installation de sequencage de l'adn
US5061361A (en) * 1989-03-06 1991-10-29 Hewlett-Packard Company Capillary zone electrophoresis cell system
FR2649487B1 (fr) * 1989-06-21 1991-09-20 Europhor Sa Capillaire d'electrophorese a dispositif optique incorpore
US5274240A (en) * 1990-01-12 1993-12-28 The Regents Of The University Of California Capillary array confocal fluorescence scanner and method
FR2671407B1 (fr) * 1991-01-08 1994-08-05 Europhor Sa Procede d'analyse par electrophorese capillaire avec detection par fluorescence, et dispositifs de mise en óoeuvre.
US5203339A (en) * 1991-06-28 1993-04-20 The Government Of The United States Of America As Represented By The Secretary Of The Department Health And Human Services Method and apparatus for imaging a physical parameter in turbid media using diffuse waves
US5273638A (en) * 1991-09-30 1993-12-28 Beckman Instruments, Inc. Nucleotide sequence determination employing matched dideoxynucleotide terminator concentrations
EP0539743B1 (fr) * 1991-09-30 1997-08-06 Beckman Instruments, Inc. Détection améliorée de la fluorescence d'échantillons dans une colonne capillaire
US5484571A (en) * 1991-10-08 1996-01-16 Beckman Instruments, Inc. Enhanced fluorescence detection of samples in capillary column
AU3816993A (en) * 1992-03-19 1993-10-21 Regents Of The University Of California, The Multiple tag labeling method for DNA sequencing
JPH05332992A (ja) * 1992-05-29 1993-12-17 Shimadzu Corp 電気泳動装置
DE4336911C2 (de) * 1993-10-28 2002-11-21 Biotechnolog Forschung Gmbh Verfahren zur Primer-Herstellung und Template-Bank dafür
JP3068413B2 (ja) * 1994-07-13 2000-07-24 日立電子エンジニアリング株式会社 Ｄｎａ塩基配列決定装置
US5675155A (en) * 1995-04-26 1997-10-07 Beckman Instruments, Inc. Multicapillary fluorescent detection system
US6110683A (en) * 1999-01-08 2000-08-29 Commonwealth Biotechnologies, Inc. Automated DNA Sequencer loading dye which contains a lane tracking aid

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1205028A (fr) * 1981-07-01 1986-05-27 Jerald C. Hinshaw Chelates fluorescents et reactifs de liaison specifique marques prepares a partir desdits chelates
CA1180647A (fr) * 1981-07-17 1985-01-08 Cavit Akin Methode de diagnostic d'hybridation de polynucleotides par emission de lumiere
FR2519005B1 (fr) * 1981-12-29 1985-10-25 Pasteur Institut Fragments d'adn marques a l'une au moins de leurs extremites par des ribonucleotides modifies reconnaissables par des molecules affines et procede pour realiser une analyse de tels fragments d'adn
DK105582A (da) * 1982-03-11 1983-09-12 Nordisk Insulinlab Fremgangsmaade til bestemmelse af humane hla-d(r) vaevstyper og gensonde til brug ved fremgangsmaaden
US4490472A (en) * 1982-06-17 1984-12-25 Imreg, Inc. Sensitive tests for malignancies based on DNA detection

Also Published As

Publication number Publication date
SE8500201D0 (sv) 1985-01-16
DE3501306A1 (de) 1985-07-25
FR2558262B1 (fr) 1989-04-07
FR2558262A1 (fr) 1985-07-19
GB2155176A (en) 1985-09-18
SE456348B (sv) 1988-09-26
DE3501306C2 (de) 1998-09-24
SE8500201L (sv) 1985-07-17
GB8500960D0 (en) 1985-02-20
GB2155176B (en) 1988-05-11

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Legal Events

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