CA1244786A - Synthesis of amino-derivitized oligonucleotides - Google Patents

Synthesis of amino-derivitized oligonucleotides

Info

Publication number
CA1244786A
CA1244786A CA000470476A CA470476A CA1244786A CA 1244786 A CA1244786 A CA 1244786A CA 000470476 A CA000470476 A CA 000470476A CA 470476 A CA470476 A CA 470476A CA 1244786 A CA1244786 A CA 1244786A
Authority
CA
Canada
Prior art keywords
product
amino group
oligonucleotides
oligonucleotide
detectable moiety
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
CA000470476A
Other languages
English (en)
French (fr)
Inventor
Lloyd M. Smith
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
California Institute of Technology CalTech
Original Assignee
California Institute of Technology CalTech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by California Institute of Technology CalTech filed Critical California Institute of Technology CalTech
Application granted granted Critical
Publication of CA1244786A publication Critical patent/CA1244786A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
CA000470476A 1983-12-20 1984-12-19 Synthesis of amino-derivitized oligonucleotides Expired CA1244786A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US56501083A 1983-12-20 1983-12-20
US565,010 1990-08-09

Publications (1)

Publication Number Publication Date
CA1244786A true CA1244786A (en) 1988-11-15

Family

ID=24256835

Family Applications (1)

Application Number Title Priority Date Filing Date
CA000470476A Expired CA1244786A (en) 1983-12-20 1984-12-19 Synthesis of amino-derivitized oligonucleotides

Country Status (6)

Country Link
JP (3) JPS60197698A (de)
CA (1) CA1244786A (de)
DE (1) DE3446635C2 (de)
FR (1) FR2556726B1 (de)
GB (1) GB2153356B (de)
SE (1) SE466208B (de)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1223831A (en) 1982-06-23 1987-07-07 Dean Engelhardt Modified nucleotides, methods of preparing and utilizing and compositions containing the same
US4849513A (en) * 1983-12-20 1989-07-18 California Institute Of Technology Deoxyribonucleoside phosphoramidites in which an aliphatic amino group is attached to the sugar ring and their use for the preparation of oligonucleotides containing aliphatic amino groups
US5118800A (en) * 1983-12-20 1992-06-02 California Institute Of Technology Oligonucleotides possessing a primary amino group in the terminal nucleotide
US5015733A (en) * 1983-12-20 1991-05-14 California Institute Of Technology Nucleosides possessing blocked aliphatic amino groups
USRE43096E1 (en) 1984-01-16 2012-01-10 California Institute Of Technology Tagged extendable primers and extension products
US5821058A (en) 1984-01-16 1998-10-13 California Institute Of Technology Automated DNA sequencing technique
GB8509880D0 (en) * 1985-04-17 1985-05-22 Ici Plc Testing device
US4762779A (en) * 1985-06-13 1988-08-09 Amgen Inc. Compositions and methods for functionalizing nucleic acids
US4757141A (en) * 1985-08-26 1988-07-12 Applied Biosystems, Incorporated Amino-derivatized phosphite and phosphate linking agents, phosphoramidite precursors, and useful conjugates thereof
US4959463A (en) * 1985-10-15 1990-09-25 Genentech, Inc. Intermediates
US4855225A (en) * 1986-02-07 1989-08-08 Applied Biosystems, Inc. Method of detecting electrophoretically separated oligonucleotides
DE3642939A1 (de) * 1986-06-03 1987-12-10 Europ Lab Molekularbiolog Verfahren zur dna-markierung
CA1340806C (en) * 1986-07-02 1999-11-02 James Merrill Prober Method, system and reagents for dna sequencing
US5242796A (en) * 1986-07-02 1993-09-07 E. I. Du Pont De Nemours And Company Method, system and reagents for DNA sequencing
US4904582A (en) * 1987-06-11 1990-02-27 Synthetic Genetics Novel amphiphilic nucleic acid conjugates
US4833332A (en) * 1987-06-12 1989-05-23 E. I. Du Pont De Nemours And Company Scanning fluorescent detection system
US4965349A (en) * 1987-12-24 1990-10-23 Applied Biosystems, Inc. Method of synthesizing oligonucleotides labeled with ammonia-labile groups on solid phase supports
US4997928A (en) * 1988-09-15 1991-03-05 E. I. Du Pont De Nemours And Company Fluorescent reagents for the preparation of 5'-tagged oligonucleotides
US5262536A (en) * 1988-09-15 1993-11-16 E. I. Du Pont De Nemours And Company Reagents for the preparation of 5'-tagged oligonucleotides
GB8822228D0 (en) * 1988-09-21 1988-10-26 Southern E M Support-bound oligonucleotides
WO1992010505A1 (en) * 1990-12-11 1992-06-25 Abbott Laboratories Methods for labelling oligonucleotides
US5643722A (en) * 1994-05-11 1997-07-01 Trustees Of Boston University Methods for the detection and isolation of proteins
US6020481A (en) * 1996-04-01 2000-02-01 The Perkin-Elmer Corporation Asymmetric benzoxanthene dyes
SE522077C2 (sv) 1997-09-05 2004-01-13 Lightup Technologies Ab Förfarande att välja sekvens hos sond för nukleinsyrahybridisering
DE19815864A1 (de) 1998-04-08 1999-10-14 Europ Lab Molekularbiolog 5'-modifizierte Nukleotide und ihre Anwendung in der Molekularbiologie und Medizin
AU2998800A (en) 1999-03-24 2000-10-09 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The N-acylphosphoramidites and their use in oligonucleotide synthesis
US7355037B2 (en) 2001-12-03 2008-04-08 The United States Of America As Represented By The Department Of Health And Human Services Thermolabile hydroxyl protecting groups and methods of use
US9809824B2 (en) 2004-12-13 2017-11-07 The United States Of America, Represented By The Secretary, Department Of Health And Human Services CpG oligonucleotide prodrugs, compositions thereof and associated therapeutic methods

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3284440A (en) * 1964-06-12 1966-11-08 Merck & Co Inc Phosphate esters of cytosine arabinoide and process for preparing same
US3847898A (en) * 1969-05-27 1974-11-12 Upjohn Co N4-trihaloethoxy carbonyl arabino-furanosyl cytosine 5'-esters
IL57570A (en) * 1978-06-22 1982-07-30 Miles Lab Method and reagent for specific binding assay with a prosthetic group as a label component
IL57571A (en) * 1978-06-22 1983-03-31 Miles Lab Flavin adenine dinucleotide-labeled conjugates and their use in a homogeneous immunoassay method for determining haptens
US4415732A (en) * 1981-03-27 1983-11-15 University Patents, Inc. Phosphoramidite compounds and processes
FR2519005B1 (fr) * 1981-12-29 1985-10-25 Pasteur Institut Fragments d'adn marques a l'une au moins de leurs extremites par des ribonucleotides modifies reconnaissables par des molecules affines et procede pour realiser une analyse de tels fragments d'adn

Also Published As

Publication number Publication date
GB2153356B (en) 1987-07-22
SE8406484L (sv) 1985-06-21
DE3446635A1 (de) 1985-06-27
SE8406484D0 (sv) 1984-12-19
JPH0157119B2 (de) 1989-12-04
DE3446635C2 (de) 1997-08-07
FR2556726B1 (fr) 1987-02-20
JPS60197698A (ja) 1985-10-07
GB8432205D0 (en) 1985-01-30
GB2153356A (en) 1985-08-21
JPH0678353B2 (ja) 1994-10-05
JPH0460600B2 (de) 1992-09-28
FR2556726A1 (fr) 1985-06-21
JPH06145192A (ja) 1994-05-24
JPH01250393A (ja) 1989-10-05
SE466208B (sv) 1992-01-13

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