CA1173379A - Cell lines - Google Patents
Cell linesInfo
- Publication number
- CA1173379A CA1173379A CA000381131A CA381131A CA1173379A CA 1173379 A CA1173379 A CA 1173379A CA 000381131 A CA000381131 A CA 000381131A CA 381131 A CA381131 A CA 381131A CA 1173379 A CA1173379 A CA 1173379A
- Authority
- CA
- Canada
- Prior art keywords
- cell line
- cells
- rat
- hybrid
- myeloma cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 206010035226 Plasma cell myeloma Diseases 0.000 claims abstract description 50
- 201000000050 myeloid neoplasm Diseases 0.000 claims abstract description 50
- 238000010367 cloning Methods 0.000 claims abstract description 18
- 241001465754 Metazoa Species 0.000 claims abstract description 14
- 108060003951 Immunoglobulin Proteins 0.000 claims abstract description 11
- 230000002163 immunogen Effects 0.000 claims abstract description 11
- 102000018358 immunoglobulin Human genes 0.000 claims abstract description 11
- 210000004027 cell Anatomy 0.000 claims description 250
- 241000700159 Rattus Species 0.000 claims description 97
- 239000002609 medium Substances 0.000 claims description 59
- 238000000034 method Methods 0.000 claims description 54
- 210000002966 serum Anatomy 0.000 claims description 32
- 238000004519 manufacturing process Methods 0.000 claims description 28
- 206010028980 Neoplasm Diseases 0.000 claims description 25
- 230000004927 fusion Effects 0.000 claims description 24
- 230000008569 process Effects 0.000 claims description 21
- 238000001727 in vivo Methods 0.000 claims description 17
- 239000001963 growth medium Substances 0.000 claims description 14
- 229920001817 Agar Polymers 0.000 claims description 13
- 239000008272 agar Substances 0.000 claims description 13
- 238000000338 in vitro Methods 0.000 claims description 10
- 239000003795 chemical substances by application Substances 0.000 claims description 8
- 238000002360 preparation method Methods 0.000 claims description 8
- 210000004989 spleen cell Anatomy 0.000 claims description 8
- 210000003567 ascitic fluid Anatomy 0.000 claims description 7
- 229920001223 polyethylene glycol Polymers 0.000 claims description 6
- 239000002202 Polyethylene glycol Substances 0.000 claims description 5
- 238000012258 culturing Methods 0.000 claims description 4
- 235000015097 nutrients Nutrition 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 4
- 230000007910 cell fusion Effects 0.000 claims description 3
- 239000000427 antigen Substances 0.000 claims description 2
- 102000036639 antigens Human genes 0.000 claims description 2
- 108091007433 antigens Proteins 0.000 claims description 2
- 238000009396 hybridization Methods 0.000 claims 3
- 238000012606 in vitro cell culture Methods 0.000 claims 2
- 210000004408 hybridoma Anatomy 0.000 description 19
- 210000004754 hybrid cell Anatomy 0.000 description 14
- 239000000725 suspension Substances 0.000 description 11
- 241000699666 Mus <mouse, genus> Species 0.000 description 10
- LPXQRXLUHJKZIE-UHFFFAOYSA-N 8-azaguanine Chemical compound NC1=NC(O)=C2NN=NC2=N1 LPXQRXLUHJKZIE-UHFFFAOYSA-N 0.000 description 8
- 229960005508 8-azaguanine Drugs 0.000 description 8
- 102000004243 Tubulin Human genes 0.000 description 8
- 108090000704 Tubulin Proteins 0.000 description 8
- 230000008901 benefit Effects 0.000 description 8
- 210000000952 spleen Anatomy 0.000 description 8
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 7
- 239000006228 supernatant Substances 0.000 description 7
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 239000007924 injection Substances 0.000 description 6
- 238000002347 injection Methods 0.000 description 6
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 6
- 241001494479 Pecora Species 0.000 description 5
- 239000002671 adjuvant Substances 0.000 description 5
- 244000309466 calf Species 0.000 description 5
- 238000004113 cell culture Methods 0.000 description 5
- 230000016784 immunoglobulin production Effects 0.000 description 5
- 229940090044 injection Drugs 0.000 description 5
- 230000003834 intracellular effect Effects 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 4
- 239000004472 Lysine Substances 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 230000001143 conditioned effect Effects 0.000 description 4
- 238000010790 dilution Methods 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 238000002649 immunization Methods 0.000 description 4
- 238000010348 incorporation Methods 0.000 description 4
- 239000004615 ingredient Substances 0.000 description 4
- 238000002955 isolation Methods 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- 239000004033 plastic Substances 0.000 description 4
- 229920003023 plastic Polymers 0.000 description 4
- XOJVVFBFDXDTEG-UHFFFAOYSA-N pristane Chemical compound CC(C)CCCC(C)CCCC(C)CCCC(C)C XOJVVFBFDXDTEG-UHFFFAOYSA-N 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 230000035945 sensitivity Effects 0.000 description 4
- 239000012090 serum-supplement Substances 0.000 description 4
- DAEPDZWVDSPTHF-UHFFFAOYSA-M sodium pyruvate Chemical compound [Na+].CC(=O)C([O-])=O DAEPDZWVDSPTHF-UHFFFAOYSA-M 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 210000004881 tumor cell Anatomy 0.000 description 4
- 101000901154 Homo sapiens Complement C3 Proteins 0.000 description 3
- 241000699670 Mus sp. Species 0.000 description 3
- 241000283973 Oryctolagus cuniculus Species 0.000 description 3
- 229930182555 Penicillin Natural products 0.000 description 3
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 230000000890 antigenic effect Effects 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 210000003743 erythrocyte Anatomy 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 229940049954 penicillin Drugs 0.000 description 3
- 239000002953 phosphate buffered saline Substances 0.000 description 3
- 238000000159 protein binding assay Methods 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 229960005322 streptomycin Drugs 0.000 description 3
- 238000010254 subcutaneous injection Methods 0.000 description 3
- 239000007929 subcutaneous injection Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000013589 supplement Substances 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- SGTNSNPWRIOYBX-UHFFFAOYSA-N 2-(3,4-dimethoxyphenyl)-5-{[2-(3,4-dimethoxyphenyl)ethyl](methyl)amino}-2-(propan-2-yl)pentanenitrile Chemical compound C1=C(OC)C(OC)=CC=C1CCN(C)CCCC(C#N)(C(C)C)C1=CC=C(OC)C(OC)=C1 SGTNSNPWRIOYBX-UHFFFAOYSA-N 0.000 description 2
- 206010003445 Ascites Diseases 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 2
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
- 229920001202 Inulin Polymers 0.000 description 2
- 206010070834 Sensitisation Diseases 0.000 description 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 2
- 230000004520 agglutination Effects 0.000 description 2
- 239000003708 ampul Substances 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 230000003750 conditioning effect Effects 0.000 description 2
- 229960001760 dimethyl sulfoxide Drugs 0.000 description 2
- 230000009429 distress Effects 0.000 description 2
- 238000000799 fluorescence microscopy Methods 0.000 description 2
- 210000005260 human cell Anatomy 0.000 description 2
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 2
- FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 238000007912 intraperitoneal administration Methods 0.000 description 2
- JYJIGFIDKWBXDU-MNNPPOADSA-N inulin Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@]1(OC[C@]2(OC[C@]3(OC[C@]4(OC[C@]5(OC[C@]6(OC[C@]7(OC[C@]8(OC[C@]9(OC[C@]%10(OC[C@]%11(OC[C@]%12(OC[C@]%13(OC[C@]%14(OC[C@]%15(OC[C@]%16(OC[C@]%17(OC[C@]%18(OC[C@]%19(OC[C@]%20(OC[C@]%21(OC[C@]%22(OC[C@]%23(OC[C@]%24(OC[C@]%25(OC[C@]%26(OC[C@]%27(OC[C@]%28(OC[C@]%29(OC[C@]%30(OC[C@]%31(OC[C@]%32(OC[C@]%33(OC[C@]%34(OC[C@]%35(OC[C@]%36(O[C@@H]%37[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O%37)O)[C@H]([C@H](O)[C@@H](CO)O%36)O)[C@H]([C@H](O)[C@@H](CO)O%35)O)[C@H]([C@H](O)[C@@H](CO)O%34)O)[C@H]([C@H](O)[C@@H](CO)O%33)O)[C@H]([C@H](O)[C@@H](CO)O%32)O)[C@H]([C@H](O)[C@@H](CO)O%31)O)[C@H]([C@H](O)[C@@H](CO)O%30)O)[C@H]([C@H](O)[C@@H](CO)O%29)O)[C@H]([C@H](O)[C@@H](CO)O%28)O)[C@H]([C@H](O)[C@@H](CO)O%27)O)[C@H]([C@H](O)[C@@H](CO)O%26)O)[C@H]([C@H](O)[C@@H](CO)O%25)O)[C@H]([C@H](O)[C@@H](CO)O%24)O)[C@H]([C@H](O)[C@@H](CO)O%23)O)[C@H]([C@H](O)[C@@H](CO)O%22)O)[C@H]([C@H](O)[C@@H](CO)O%21)O)[C@H]([C@H](O)[C@@H](CO)O%20)O)[C@H]([C@H](O)[C@@H](CO)O%19)O)[C@H]([C@H](O)[C@@H](CO)O%18)O)[C@H]([C@H](O)[C@@H](CO)O%17)O)[C@H]([C@H](O)[C@@H](CO)O%16)O)[C@H]([C@H](O)[C@@H](CO)O%15)O)[C@H]([C@H](O)[C@@H](CO)O%14)O)[C@H]([C@H](O)[C@@H](CO)O%13)O)[C@H]([C@H](O)[C@@H](CO)O%12)O)[C@H]([C@H](O)[C@@H](CO)O%11)O)[C@H]([C@H](O)[C@@H](CO)O%10)O)[C@H]([C@H](O)[C@@H](CO)O9)O)[C@H]([C@H](O)[C@@H](CO)O8)O)[C@H]([C@H](O)[C@@H](CO)O7)O)[C@H]([C@H](O)[C@@H](CO)O6)O)[C@H]([C@H](O)[C@@H](CO)O5)O)[C@H]([C@H](O)[C@@H](CO)O4)O)[C@H]([C@H](O)[C@@H](CO)O3)O)[C@H]([C@H](O)[C@@H](CO)O2)O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JYJIGFIDKWBXDU-MNNPPOADSA-N 0.000 description 2
- 229940029339 inulin Drugs 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 210000004698 lymphocyte Anatomy 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000008188 pellet Substances 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 230000008313 sensitization Effects 0.000 description 2
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 2
- 229940054269 sodium pyruvate Drugs 0.000 description 2
- 238000004114 suspension culture Methods 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- LRZPQLZONWIQOJ-UHFFFAOYSA-N 10-(2-methylprop-2-enoyloxy)decyl 2-methylprop-2-enoate Chemical compound CC(=C)C(=O)OCCCCCCCCCCOC(=O)C(C)=C LRZPQLZONWIQOJ-UHFFFAOYSA-N 0.000 description 1
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
- BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 description 1
- NJXWZWXCHBNOOG-UHFFFAOYSA-N 3,3-diphenylpropyl(1-phenylethyl)azanium;chloride Chemical compound [Cl-].C=1C=CC=CC=1C(C)[NH2+]CCC(C=1C=CC=CC=1)C1=CC=CC=C1 NJXWZWXCHBNOOG-UHFFFAOYSA-N 0.000 description 1
- TVZGACDUOSZQKY-LBPRGKRZSA-N 4-aminofolic acid Chemical compound C1=NC2=NC(N)=NC(N)=C2N=C1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 TVZGACDUOSZQKY-LBPRGKRZSA-N 0.000 description 1
- 101100230376 Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) celI gene Proteins 0.000 description 1
- 206010002091 Anaesthesia Diseases 0.000 description 1
- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 241000700199 Cavia porcellus Species 0.000 description 1
- 229910021555 Chromium Chloride Inorganic materials 0.000 description 1
- 102100022133 Complement C3 Human genes 0.000 description 1
- 108010078015 Complement C3b Proteins 0.000 description 1
- 241000518994 Conta Species 0.000 description 1
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 1
- 239000006145 Eagle's minimal essential medium Substances 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- UYTPUPDQBNUYGX-UHFFFAOYSA-N Guanine Natural products O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 1
- 208000032843 Hemorrhage Diseases 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 description 1
- 108010079585 Immunoglobulin Subunits Proteins 0.000 description 1
- 102000012745 Immunoglobulin Subunits Human genes 0.000 description 1
- 241001314546 Microtis <orchid> Species 0.000 description 1
- 241000711408 Murine respirovirus Species 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- BELBBZDIHDAJOR-UHFFFAOYSA-N Phenolsulfonephthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2S(=O)(=O)O1 BELBBZDIHDAJOR-UHFFFAOYSA-N 0.000 description 1
- 108010033737 Pokeweed Mitogens Proteins 0.000 description 1
- 241000276498 Pollachius virens Species 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 229920002535 Polyethylene Glycol 1500 Polymers 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 239000012980 RPMI-1640 medium Substances 0.000 description 1
- 239000006146 Roswell Park Memorial Institute medium Substances 0.000 description 1
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 1
- QTENRWWVYAAPBI-YZTFXSNBSA-N Streptomycin sulfate Chemical compound OS(O)(=O)=O.OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@H]1[C@H](N=C(N)N)[C@@H](O)[C@H](N=C(N)N)[C@@H](O)[C@@H]1O.CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@H]1[C@H](N=C(N)N)[C@@H](O)[C@H](N=C(N)N)[C@@H](O)[C@@H]1O QTENRWWVYAAPBI-YZTFXSNBSA-N 0.000 description 1
- 241000950638 Symphysodon discus Species 0.000 description 1
- 239000004809 Teflon Substances 0.000 description 1
- 229920006362 Teflon® Polymers 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 210000000683 abdominal cavity Anatomy 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 229960003896 aminopterin Drugs 0.000 description 1
- 238000001949 anaesthesia Methods 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 210000000628 antibody-producing cell Anatomy 0.000 description 1
- 230000007503 antigenic stimulation Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- QSWDMMVNRMROPK-UHFFFAOYSA-K chromium(3+) trichloride Chemical compound [Cl-].[Cl-].[Cl-].[Cr+3] QSWDMMVNRMROPK-UHFFFAOYSA-K 0.000 description 1
- 230000024203 complement activation Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 238000009795 derivation Methods 0.000 description 1
- XAEWZDYWZHIUCT-UHFFFAOYSA-N desipramine hydrochloride Chemical compound [H+].[Cl-].C1CC2=CC=CC=C2N(CCCNC)C2=CC=CC=C21 XAEWZDYWZHIUCT-UHFFFAOYSA-N 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 description 1
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 239000012737 fresh medium Substances 0.000 description 1
- 239000012520 frozen sample Substances 0.000 description 1
- 108010074605 gamma-Globulins Proteins 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- IVSXFFJGASXYCL-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=NC=N[C]21 IVSXFFJGASXYCL-UHFFFAOYSA-N 0.000 description 1
- 230000035931 haemagglutination Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000002489 hematologic effect Effects 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 239000003018 immunosuppressive agent Substances 0.000 description 1
- 229940124589 immunosuppressive drug Drugs 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 238000010255 intramuscular injection Methods 0.000 description 1
- 239000007927 intramuscular injection Substances 0.000 description 1
- 239000007928 intraperitoneal injection Substances 0.000 description 1
- 238000001990 intravenous administration Methods 0.000 description 1
- 238000010253 intravenous injection Methods 0.000 description 1
- HOQADATXFBOEGG-UHFFFAOYSA-N isofenphos Chemical compound CCOP(=S)(NC(C)C)OC1=CC=CC=C1C(=O)OC(C)C HOQADATXFBOEGG-UHFFFAOYSA-N 0.000 description 1
- 210000002751 lymph Anatomy 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000007758 minimum essential medium Substances 0.000 description 1
- 230000001613 neoplastic effect Effects 0.000 description 1
- 239000000712 neurohormone Substances 0.000 description 1
- 239000002858 neurotransmitter agent Substances 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 229960003531 phenolsulfonphthalein Drugs 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- -1 polyethylene Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 230000003334 potential effect Effects 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000010079 rubber tapping Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 235000019333 sodium laurylsulphate Nutrition 0.000 description 1
- 238000009987 spinning Methods 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- VUYXVWGKCKTUMF-UHFFFAOYSA-N tetratriacontaethylene glycol monomethyl ether Chemical compound COCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCO VUYXVWGKCKTUMF-UHFFFAOYSA-N 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 229940104230 thymidine Drugs 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/14—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from fungi, algea or lichens
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
- C12N5/12—Fused cells, e.g. hybridomas
- C12N5/16—Animal cells
- C12N5/163—Animal cells one of the fusion partners being a B or a T lymphocyte
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/948—Microorganisms using viruses or cell lines
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Compounds Of Unknown Constitution (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB8022206 | 1980-07-07 | ||
| GB8022206 | 1980-07-07 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1173379A true CA1173379A (en) | 1984-08-28 |
Family
ID=10514589
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000381131A Expired CA1173379A (en) | 1980-07-07 | 1981-07-06 | Cell lines |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US4472500A (OSRAM) |
| EP (1) | EP0043718B1 (OSRAM) |
| JP (3) | JPS5739777A (OSRAM) |
| CA (1) | CA1173379A (OSRAM) |
| DE (1) | DE3167442D1 (OSRAM) |
Families Citing this family (109)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CH652145A5 (de) * | 1982-01-22 | 1985-10-31 | Sandoz Ag | Verfahren zur in vitro-herstellung von hybridomen welche humane monoklonale antikoerper erzeugen. |
| US4987079A (en) * | 1982-07-06 | 1991-01-22 | Wilbur D. Smith | Composition and method for in vitro cell culture |
| JPH0679553B2 (ja) * | 1983-01-20 | 1994-10-12 | サントリー株式会社 | 新規な雑種細胞 |
| GB8318575D0 (en) * | 1983-07-08 | 1983-08-10 | Cobbold S P | Antibody preparations |
| US5639639A (en) * | 1983-11-02 | 1997-06-17 | Genzyme Corporation | Recombinant heterodimeric human fertility hormones, and methods, cells, vectors and DNA for the production thereof |
| US4634666A (en) * | 1984-01-06 | 1987-01-06 | The Board Of Trustees Of The Leland Stanford Junior University | Human-murine hybridoma fusion partner |
| US4562003A (en) * | 1984-10-26 | 1985-12-31 | California Biotechnology, Inc. | Monoclonal antibodies against alveolar surfactant protein |
| US5981216A (en) * | 1985-04-01 | 1999-11-09 | Alusuisse Holdings A.G. | Transformed myeloma cell-line and a process for the expression of a gene coding for a eukaryotic polypeptide employing same |
| EP0216846B2 (en) * | 1985-04-01 | 1995-04-26 | Celltech Limited | Transformed myeloma cell-line and a process for the expression of a gene coding for a eukaryotic polypeptide employing same |
| US4808532A (en) * | 1985-07-01 | 1989-02-28 | The United States Of America As Represented By The United States Department Of Energy | Continuous human cell lines and method of making same |
| US5292636A (en) * | 1986-03-31 | 1994-03-08 | T Cell Diagnostics, Inc. | Therapeutic and diagnostic methods using soluble T cell surface molecules |
| JPS63180059U (OSRAM) * | 1987-05-11 | 1988-11-21 | ||
| EP0329184A3 (en) * | 1988-02-19 | 1990-05-23 | Neorx Corporation | Antimers and antimeric conjugation |
| JPH04500970A (ja) | 1988-09-02 | 1992-02-20 | ザ ロックフェラー ユニヴァーシティ | マクロファージ由来炎症メディエーター （ｍｉｐ―２） |
| CA2002067A1 (en) * | 1988-11-09 | 1990-05-09 | Tamotsu Fukuda | Parent cell line for producing human hybridomas |
| JPH0727946Y2 (ja) * | 1989-03-14 | 1995-06-28 | トーコー衛材株式会社 | 簡易マスク |
| US5567594A (en) * | 1991-04-26 | 1996-10-22 | Enteron, L.P. | Methods and compositions for the detection and treatment of diseases associated with antigens of microorganisms |
| US6190864B1 (en) | 1991-05-08 | 2001-02-20 | Chiron Corporation | HCV genomic sequences for diagnostics and therapeutics |
| ATE249838T1 (de) | 1993-05-12 | 2003-10-15 | Chiron Corp | Konserviertes motiv der hepatitis c virus e2/ns1 region |
| US6309853B1 (en) | 1994-08-17 | 2001-10-30 | The Rockfeller University | Modulators of body weight, corresponding nucleic acids and proteins, and diagnostic and therapeutic uses thereof |
| US6048837A (en) * | 1994-08-17 | 2000-04-11 | The Rockefeller University | OB polypeptides as modulators of body weight |
| US6350730B1 (en) | 1994-08-17 | 2002-02-26 | The Rockefeller University | OB polypeptides and modified forms as modulators of body weight |
| US6471956B1 (en) | 1994-08-17 | 2002-10-29 | The Rockefeller University | Ob polypeptides, modified forms and compositions thereto |
| US6124439A (en) * | 1994-08-17 | 2000-09-26 | The Rockefeller University | OB polypeptide antibodies and method of making |
| US6124448A (en) * | 1994-08-17 | 2000-09-26 | The Rockfeller University | Nucleic acid primers and probes for the mammalian OB gene |
| US6001968A (en) * | 1994-08-17 | 1999-12-14 | The Rockefeller University | OB polypeptides, modified forms and compositions |
| US5759776A (en) * | 1995-06-05 | 1998-06-02 | California Pacific Medical Center | Targets for breast cancer diagnosis and treatment |
| US5776683A (en) * | 1996-07-11 | 1998-07-07 | California Pacific Medical Center | Methods for identifying genes amplified in cancer cells |
| US6893636B2 (en) * | 1997-02-20 | 2005-05-17 | Biogen Idec Ma Inc. | Gamma-1 and gamma-3 anti-human CD23 monoclonal antibodies and use thereof as therapeutics |
| US7314711B2 (en) | 1997-05-23 | 2008-01-01 | Bioveris Corporation | Assays employing electrochemiluminescent labels and electrochemiluminescence quenchers |
| US6528271B1 (en) * | 1997-06-05 | 2003-03-04 | Duke University | Inhibition of βarrestin mediated effects prolongs and potentiates opioid receptor-mediated analgesia |
| US5891646A (en) * | 1997-06-05 | 1999-04-06 | Duke University | Methods of assaying receptor activity and constructs useful in such methods |
| US7541151B2 (en) | 1997-06-05 | 2009-06-02 | Duke University | Single-cell biosensor for the measurement of GPCR ligands in a test sample |
| EP1816200B1 (en) | 1997-12-11 | 2016-03-09 | Merial | Postweaning multisystemic wasting syndrome virus for pigs |
| ATE364624T1 (de) | 1997-12-11 | 2007-07-15 | Univ Saskatchewan | Multisystemisches kümmerwuchssyndrom durch viren in schweine |
| US6087128A (en) * | 1998-02-12 | 2000-07-11 | Ndsu Research Foundation | DNA encoding an avian E. coli iss |
| US6369201B1 (en) | 1998-02-19 | 2002-04-09 | Metamorphix International, Inc. | Myostatin multimers |
| WO2000016804A1 (en) * | 1998-09-18 | 2000-03-30 | Dynavax Technologies Corporation | METHODS OF TREATING IgE-ASSOCIATED DISORDERS AND COMPOSITIONS FOR USE THEREIN |
| US6667176B1 (en) | 2000-01-11 | 2003-12-23 | Geron Corporation | cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells |
| US6773911B1 (en) | 1998-11-23 | 2004-08-10 | Amgen Canada Inc. | Apoptosis-inducing factor |
| US6156514A (en) * | 1998-12-03 | 2000-12-05 | Sunol Molecular Corporation | Methods for making recombinant cells |
| ES2350306T3 (es) | 1999-02-26 | 2011-01-20 | Novartis Vaccines And Diagnostics, Inc. | Uso de bioadhesivos y adyuvantes para la administración de antígenos por vía mucosa. |
| US6399759B1 (en) | 1999-05-27 | 2002-06-04 | University Of Georgia Research Foundation, Inc. | Ant proteases and methods of inhibition |
| US6262265B1 (en) | 1999-06-18 | 2001-07-17 | Microgenics Corporation | Non-hydrolyzable analogs of heroin metabolites suitable for use in immunoassay |
| DE60041821D1 (de) | 1999-09-24 | 2009-04-30 | Cybios Llc | Pluripotent embryonale stammzellen-ähnliche zellen, zusammensetzungen und ihre vervendungen |
| DE19957065B4 (de) | 1999-11-26 | 2005-01-05 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Screening-Verfahren für Arzneistoffe |
| US20020064861A1 (en) * | 2000-01-11 | 2002-05-30 | Barletta Raul G. | Identification of virulence determinants |
| AU2016204535C1 (en) * | 2000-04-12 | 2017-02-23 | Laboratoire Français Du Fractionnement Et Des Biotechnologies | Anti-D monoclonal antibodies |
| FR2807767B1 (fr) | 2000-04-12 | 2005-01-14 | Lab Francais Du Fractionnement | Anticorps monoclonaux anti-d |
| US6458589B1 (en) | 2000-04-27 | 2002-10-01 | Geron Corporation | Hepatocyte lineage cells derived from pluripotent stem cells |
| ATE536375T1 (de) | 2000-05-10 | 2011-12-15 | Mayo Foundation | Menschliche igm antikörper, welche die fähigkeit besitzen, den wiederaufbau der myelinstruktur zu induzieren und deren diagnostische und therapeutische anwendung im zentralen nervensystem |
| US7250294B2 (en) | 2000-05-17 | 2007-07-31 | Geron Corporation | Screening small molecule drugs using neural cells differentiated from human embryonic stem cells |
| EP1734050A3 (en) | 2000-06-12 | 2012-12-05 | University Of Saskatchewan | Immunization of dairy cattle with GapC protein against streptococcus infection |
| US7163800B2 (en) * | 2000-11-03 | 2007-01-16 | Molecular Devices Corporation | Methods of screening compositions for G protein-coupled receptor desensitization inhibitory activity |
| US7018812B2 (en) * | 2000-11-03 | 2006-03-28 | Duke University | Modified G-protein coupled receptors |
| US20050136431A1 (en) * | 2000-11-03 | 2005-06-23 | Oakley Robert H. | Methods of screening compositions for G protein-coupled receptor desensitization inhibitory activity |
| FR2817875B1 (fr) * | 2000-12-07 | 2005-03-18 | Technopharm | Procede de preparation d'un anticorps monoclonal humain, de fragments de celui-ci ou d'anticorps comprenant de tels fragments, les anticorps ainsi obtenus et leur utilisation |
| US20100056762A1 (en) | 2001-05-11 | 2010-03-04 | Old Lloyd J | Specific binding proteins and uses thereof |
| US20110313230A1 (en) | 2001-05-11 | 2011-12-22 | Terrance Grant Johns | Specific binding proteins and uses thereof |
| PT1392359E (pt) | 2001-05-11 | 2010-01-27 | Ludwig Inst For Cancer Res Ltd | Proteínas de ligação específica e suas utilizações |
| US20090324612A1 (en) * | 2001-06-05 | 2009-12-31 | Kaufman Daniel L | Modulating neuronal outgrowth via the major histocompatibility complex class i (mhc i) molecule |
| WO2002099069A2 (en) * | 2001-06-05 | 2002-12-12 | The Regents Of The University Of California | Modulating neuronal outgrowth via the major histocompatibility complex class i (mhc i) molecule |
| EP1465998A2 (en) | 2002-01-08 | 2004-10-13 | Bayer HealthCare AG | Single nucleotide polymorphisms predicting cardiovascular disease and medication efficacy |
| US20030182669A1 (en) * | 2002-03-19 | 2003-09-25 | Rockman Howard A. | Phosphoinositide 3-kinase mediated inhibition of GPCRs |
| WO2003080649A2 (en) * | 2002-03-20 | 2003-10-02 | Regents Of The University Of Minnesota | Induction of hepatocyte proliferation in vitro by inhibition of cell cycle inhibitors |
| US7290215B2 (en) * | 2002-06-03 | 2007-10-30 | Microsoft Corporation | Dynamic wizard interface system and method |
| EP1546331B2 (en) | 2002-09-26 | 2016-01-13 | K.U.Leuven Research & Development | Integrase cofactor |
| AU2002953431A0 (en) * | 2002-12-19 | 2003-01-09 | Murdoch University | BmpB Novel Nucleotide and Amino Acid Sequences and Diagnostic and Therapeutic Uses Thereof |
| US7615610B2 (en) * | 2003-01-24 | 2009-11-10 | Duke University | Modified trafficking patterns for arrestin and G-protein-coupled receptors via arrestin-ubiquitin chimera |
| EP1648427B1 (en) | 2003-05-16 | 2013-12-18 | Mayo Foundation For Medical Education And Research | Low dose IgM antibody treatment of demyelinating disorders |
| US20060035242A1 (en) | 2004-08-13 | 2006-02-16 | Michelitsch Melissa D | Prion-specific peptide reagents |
| US7767792B2 (en) | 2004-02-20 | 2010-08-03 | Ludwig Institute For Cancer Research Ltd. | Antibodies to EGF receptor epitope peptides |
| US7138504B2 (en) * | 2004-08-12 | 2006-11-21 | Microgenics Corporation | Reagents and methods for mycophenolic acid immunoassay |
| US20060240496A1 (en) * | 2005-04-21 | 2006-10-26 | Lakshmi Anne | Immunogens, derivatives and immunoassay for ethyl glucuronide |
| AR053385A1 (es) | 2005-05-12 | 2007-05-02 | Novartis Ag | Genes y proteinas novedosos de brachyspira hyodysenteriae y uso de los mismos para diagnostico y terapia |
| KR101529317B1 (ko) | 2005-06-22 | 2015-06-16 | 아스테리아스 바이오세라퓨틱스, 인크. | 영장류 다능성 줄기 세포의 심근세포 계통 세포로의 분화 |
| US7780969B2 (en) * | 2005-07-15 | 2010-08-24 | University Of Georgia Research Foundation, Inc. | Trypanosoma cruzi proteome compositions and methods |
| CN101627111A (zh) * | 2005-08-31 | 2010-01-13 | 森托科尔公司 | 用于生产具有增强的效应子功能的抗体恒定区的宿主细胞系 |
| JP2009508476A (ja) * | 2005-08-31 | 2009-03-05 | セントカー・インコーポレーテツド | 高められたエフェクター機能をもつ抗体定常領域の製造用の宿主細胞株 |
| AU2007233252C1 (en) | 2006-03-29 | 2013-05-23 | Boehringer Ingelheim Animal Health USA Inc. | Vaccine against Streptococci |
| EP2881468B1 (en) | 2006-04-21 | 2017-04-12 | Intervet International B.V. | Pestivirus species |
| EP2190481B1 (en) | 2007-07-17 | 2014-12-24 | The General Hospital Corporation | Methods to identify and enrich for populations of ovarian cancer stem cells and somatic ovarian stem cells and uses thereof |
| KR20100080591A (ko) | 2007-08-03 | 2010-07-09 | 스파이로진 피티와이 리미티드 | 브라키스피라 하이오디센테리애의 유전자와 단백질 및 이의 용도 |
| EP2363407A1 (en) | 2008-02-28 | 2011-09-07 | Murdoch University | Novel sequences of Brachyspira, immunogenic compositions, methods for preparation and use thereof |
| WO2009120891A2 (en) | 2008-03-27 | 2009-10-01 | Geron Corporation | Differentiation of primate pluripotent stem cells to hematopoietic lineage cells |
| AU2009227986C1 (en) | 2008-03-27 | 2014-06-19 | Murdoch University | Novel sequences of Brachyspira, immunogenic compositions, methods for preparation and use thereof |
| CN102724993B (zh) | 2009-04-17 | 2016-04-27 | 纽约大学 | 靶向tnf家族受体并拮抗tnf作用的肽、组合物、方法及其用途 |
| US20110076232A1 (en) | 2009-09-29 | 2011-03-31 | Ludwig Institute For Cancer Research | Specific binding proteins and uses thereof |
| WO2011084882A2 (en) | 2010-01-05 | 2011-07-14 | Contrafect Corporation | Methods and compositions for enhanced immunological therapy and targeting of gram-positive bacteria |
| WO2011113118A1 (en) | 2010-03-19 | 2011-09-22 | Katholieke Universiteit Leuven | Drug tolerance/persistence of fungal biofilms |
| JP2013534515A (ja) | 2010-06-01 | 2013-09-05 | モナシュ ユニバーシティ | プロセシングされていない受容体型チロシンキナーゼｃ−ＭＥＴに対する抗体 |
| US9297813B2 (en) | 2010-11-11 | 2016-03-29 | Agency For Science, Technology And Research | Targeting metabolic enzymes in human cancer |
| US20140329314A1 (en) | 2011-03-29 | 2014-11-06 | Christopher O'Sullivan | Enriched populations of cardiomyocyte lineage cells from pluripotent stem cells |
| GB201107467D0 (en) | 2011-05-05 | 2011-06-15 | Univ Leuven Kath | Novel treatment of pain |
| WO2014009774A1 (en) | 2012-07-12 | 2014-01-16 | Hangzhou Dac Biotech Co., Ltd | Conjugates of cell binding molecules with cytotoxic agents |
| JP6133431B2 (ja) | 2012-11-24 | 2017-05-24 | ハンジョウ ディーエーシー バイオテック シーオー．，エルティディ．Ｈａｎｇｚｈｏｕ Ｄａｃ Ｂｉｏｔｅｃｈ Ｃｏ．，Ｌｔｄ． | 親水性連結体及び薬物分子と細胞結合分子との共役反応における親水性連結体の使用 |
| WO2014209239A1 (en) | 2013-06-28 | 2014-12-31 | Singapore Health Services Pte Ltd | Trpm4 channel inhibitors for stroke treatment |
| NZ717003A (en) | 2013-09-02 | 2019-05-31 | Hangzhou Dac Biotech Co Ltd | Novel cytotoxic agents for conjugation of drugs to cell binding molecule |
| SG10201802231RA (en) | 2013-09-19 | 2018-05-30 | Univ Leland Stanford Junior | Methods and compositions for producing hepatocyte-like cells |
| US10464955B2 (en) | 2014-02-28 | 2019-11-05 | Hangzhou Dac Biotech Co., Ltd. | Charged linkers and their uses for conjugation |
| EP3265074B1 (en) | 2015-03-03 | 2020-08-12 | ARK Diagnostics, Inc. | Pregabalin immunoassays |
| EP3319936B1 (en) | 2015-07-12 | 2025-12-17 | Hangzhou Dac Biotech Co., Ltd. | Bridge linkers for conjugation of cell-binding molecules |
| US9839687B2 (en) | 2015-07-15 | 2017-12-12 | Suzhou M-Conj Biotech Co., Ltd. | Acetylenedicarboxyl linkers and their uses in specific conjugation of a cell-binding molecule |
| KR20220147721A (ko) | 2016-11-14 | 2022-11-03 | 항저우 디에이씨 바이오테크 씨오, 엘티디 | 결합 링커, 그러한 결합 링커를 함유하는 세포 결합 분자-약물 결합체, 링커를 갖는 그러한 결합체의 제조 및 사용 |
| US11578140B2 (en) | 2019-03-26 | 2023-02-14 | Microgenics Corporation | Immunoassay for mitragynine |
| BR112021025984A2 (pt) | 2019-06-29 | 2022-04-12 | Hangzhou Dac Biotech Co Ltd | Conjugado de anticorpo-derivado de tubulisina b (análogo), composto, composição farmacêutica, e, usos do conjugado, dos compostos e das composições farmacêuticas |
| CN115397848A (zh) | 2020-02-05 | 2022-11-25 | 拉利玛生物医药公司 | Tat肽结合蛋白及其用途 |
| EP3862023A1 (en) | 2020-02-05 | 2021-08-11 | Hangzhou DAC Biotech Co, Ltd | Conjugates of cell-binding molecules with cytotoxic agents |
| AU2021362997A1 (en) | 2021-11-03 | 2024-05-16 | Hangzhou Dac Biotech Co., Ltd. | Specific conjugation of an antibody |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1142466A (en) * | 1979-01-09 | 1983-03-08 | Cesar Milstein | Cell lines |
-
1981
- 1981-07-03 DE DE8181303055T patent/DE3167442D1/de not_active Expired
- 1981-07-03 EP EP81303055A patent/EP0043718B1/en not_active Expired
- 1981-07-06 CA CA000381131A patent/CA1173379A/en not_active Expired
- 1981-07-07 JP JP56106817A patent/JPS5739777A/ja active Granted
- 1981-07-07 US US06/281,038 patent/US4472500A/en not_active Expired - Lifetime
-
1990
- 1990-02-27 JP JP2047125A patent/JPH02291298A/ja active Granted
-
1992
- 1992-03-10 JP JP4051415A patent/JPH05304979A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| JPH02291298A (ja) | 1990-12-03 |
| DE3167442D1 (en) | 1985-01-10 |
| JPH0538B2 (OSRAM) | 1993-01-05 |
| JPH0372271B2 (OSRAM) | 1991-11-18 |
| US4472500A (en) | 1984-09-18 |
| EP0043718B1 (en) | 1984-11-28 |
| EP0043718A3 (en) | 1982-01-20 |
| EP0043718A2 (en) | 1982-01-13 |
| JPH05304979A (ja) | 1993-11-19 |
| JPS5739777A (en) | 1982-03-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA1173379A (en) | Cell lines | |
| US4350683A (en) | Antibody production from hybrid cell line | |
| JPH0690753A (ja) | ハイブリドーマセルラインの製造法 | |
| JPS5845407B2 (ja) | 悪性腫瘍抗体の製造方法 | |
| WO1983000503A1 (en) | Human nonsecretory plasmacytoid cell line | |
| CA1213227A (en) | Human nonsecretory plasmacytoid cell line | |
| Letchworth | Methods for production of monoclonal antibodies | |
| GB2079313A (en) | Improvements in or relating to rat myeloma cell lines | |
| WO1988000240A1 (en) | Preparation of monoclonal antibodies | |
| EP0077571A2 (en) | Process for producing a lymphokine | |
| AU636867B2 (en) | Rat monoclonal antibodies directed against human antigens and processes for preparation thereof | |
| JPH06153979A (ja) | 魚類イリドウイルスに対するモノクローナル抗体並びに該抗体を製造するためのハイブリドーマ及び方法 | |
| US4746612A (en) | Aotus interspecies hybridomas and monoclonal receptors produced thereby | |
| FI93469C (fi) | Menetelmä hybridoomasolulinjan ja sen tuottaman vasta-aineen tuottamiseksi | |
| EP0362755A2 (en) | Human cytotoxic t-cell clone and antibody thereto | |
| Sam-Yellowe | Exercise 24: Monoclonal Antibody Production | |
| WO1993012247A1 (en) | In vitro antibody production | |
| CA2085586A1 (en) | Ascites production of monoclonal antibodies using an immunosuppressive anti-cd4-related antibody | |
| RU1801117C (ru) | Способ получения монокло- нального антитела к моноклональному антителу к белку т4 лимфоцита человека, культивируемая клетка гибридомы jthfs-про- дуцентмоноклонального антитела к моноклональному антителу к белку т4 лимфоцита чело- века, культивируемая клетка гибридомы jti-if3-f5 - продуцент моноклонального антитела к моноклональному антителу к белку т4 лимфоцитачеловека, культивируемая клетка гибридомы jti-id7 - про- дуцент моноклонального антитела к моноклональному антителу к белку т4 лимфоцита человека, культивируемая клетка гибридомы jt2-n15 - продуцент моноклонального антитела к моноклональному антителу к белку т4 лимфоцитачеловека | |
| CA2039168A1 (en) | Neutralizing monoclonal antibody to infectious pancreatic necrosis virus | |
| Bodeus et al. | In Vitro Production of Rat Monoclonal Antibodies | |
| JPH06189750A (ja) | ヒト単クローン性抗肺ガン細胞抗体を産生するハイブリドーマ | |
| JPH03271235A (ja) | 抗カンジダ菌抗体分泌ハイブリドーマ | |
| JPS61238800A (ja) | モノクロ−ナル抗体ａｍｆ及びその製造法 | |
| JPH03272683A (ja) | 抗アスペルギルス菌抗体分泌ハイブリドーマ |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKEX | Expiry |