CA1167398A - Microparticles, preparation thereof and applications thereof in biology, particularly in the culture of human diploid cells - Google Patents
Microparticles, preparation thereof and applications thereof in biology, particularly in the culture of human diploid cellsInfo
- Publication number
- CA1167398A CA1167398A CA000366268A CA366268A CA1167398A CA 1167398 A CA1167398 A CA 1167398A CA 000366268 A CA000366268 A CA 000366268A CA 366268 A CA366268 A CA 366268A CA 1167398 A CA1167398 A CA 1167398A
- Authority
- CA
- Canada
- Prior art keywords
- microparticles
- protein
- particles
- reticulation
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000011859 microparticle Substances 0.000 title claims abstract description 62
- 210000001840 diploid cell Anatomy 0.000 title claims abstract description 35
- 238000002360 preparation method Methods 0.000 title description 3
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 69
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 69
- 239000002245 particle Substances 0.000 claims abstract description 56
- 239000011324 bead Substances 0.000 claims abstract description 11
- 210000004027 cell Anatomy 0.000 claims description 55
- 108010010803 Gelatin Proteins 0.000 claims description 37
- 239000008273 gelatin Substances 0.000 claims description 37
- 235000019322 gelatine Nutrition 0.000 claims description 37
- 235000011852 gelatine desserts Nutrition 0.000 claims description 37
- 229920000159 gelatin Polymers 0.000 claims description 36
- 238000000034 method Methods 0.000 claims description 31
- 230000008569 process Effects 0.000 claims description 29
- 239000003795 chemical substances by application Substances 0.000 claims description 23
- 239000000725 suspension Substances 0.000 claims description 22
- 239000000243 solution Substances 0.000 claims description 20
- 239000011248 coating agent Substances 0.000 claims description 19
- 238000000576 coating method Methods 0.000 claims description 19
- 239000001963 growth medium Substances 0.000 claims description 19
- 238000004873 anchoring Methods 0.000 claims description 18
- 230000001413 cellular effect Effects 0.000 claims description 18
- 229920005654 Sephadex Polymers 0.000 claims description 16
- 239000012507 Sephadex™ Substances 0.000 claims description 16
- 230000012010 growth Effects 0.000 claims description 16
- 125000000524 functional group Chemical group 0.000 claims description 14
- 239000002609 medium Substances 0.000 claims description 14
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical group O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 12
- 229920002307 Dextran Polymers 0.000 claims description 11
- 241000700605 Viruses Species 0.000 claims description 11
- MZVQCMJNVPIDEA-UHFFFAOYSA-N [CH2]CN(CC)CC Chemical group [CH2]CN(CC)CC MZVQCMJNVPIDEA-UHFFFAOYSA-N 0.000 claims description 11
- 238000006243 chemical reaction Methods 0.000 claims description 10
- 238000010899 nucleation Methods 0.000 claims description 10
- -1 diethyl-2-hydroxypropyl-aminoethyl substituent groups Chemical group 0.000 claims description 8
- 244000045947 parasite Species 0.000 claims description 8
- 238000004519 manufacturing process Methods 0.000 claims description 7
- 238000011534 incubation Methods 0.000 claims description 6
- 125000001309 chloro group Chemical group Cl* 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 5
- 241001465754 Metazoa Species 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 4
- 150000001299 aldehydes Chemical class 0.000 claims description 3
- 125000003636 chemical group Chemical group 0.000 claims description 3
- 125000004122 cyclic group Chemical group 0.000 claims description 3
- 230000000694 effects Effects 0.000 claims description 3
- 125000001153 fluoro group Chemical group F* 0.000 claims description 3
- 238000011533 pre-incubation Methods 0.000 claims description 3
- 210000005260 human cell Anatomy 0.000 claims description 2
- 125000000449 nitro group Chemical group [O-][N+](*)=O 0.000 claims description 2
- 229960000587 glutaral Drugs 0.000 claims 6
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims 3
- 125000001424 substituent group Chemical group 0.000 claims 3
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 claims 2
- 125000000751 azo group Chemical group [*]N=N[*] 0.000 claims 2
- 150000002466 imines Chemical class 0.000 claims 2
- 230000002411 adverse Effects 0.000 claims 1
- 239000000427 antigen Substances 0.000 claims 1
- 102000036639 antigens Human genes 0.000 claims 1
- 108091007433 antigens Proteins 0.000 claims 1
- 150000001540 azides Chemical class 0.000 claims 1
- 239000007853 buffer solution Substances 0.000 claims 1
- 239000012535 impurity Substances 0.000 claims 1
- DYMRYCZRMAHYKE-UHFFFAOYSA-N n-diazonitramide Chemical compound [O-][N+](=O)N=[N+]=[N-] DYMRYCZRMAHYKE-UHFFFAOYSA-N 0.000 claims 1
- 239000011325 microbead Substances 0.000 description 60
- 229940014259 gelatin Drugs 0.000 description 27
- 238000012360 testing method Methods 0.000 description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 9
- 239000012153 distilled water Substances 0.000 description 8
- 206010001497 Agitation Diseases 0.000 description 6
- 238000013019 agitation Methods 0.000 description 6
- 239000011806 microball Substances 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 102000004407 Lactalbumin Human genes 0.000 description 4
- 108090000942 Lactalbumin Proteins 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 239000000969 carrier Substances 0.000 description 4
- 238000007667 floating Methods 0.000 description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 3
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 102000004142 Trypsin Human genes 0.000 description 3
- 108090000631 Trypsin Proteins 0.000 description 3
- 230000000903 blocking effect Effects 0.000 description 3
- 238000004113 cell culture Methods 0.000 description 3
- 230000032823 cell division Effects 0.000 description 3
- 230000010261 cell growth Effects 0.000 description 3
- 239000000413 hydrolysate Substances 0.000 description 3
- 239000012460 protein solution Substances 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 239000012588 trypsin Substances 0.000 description 3
- 229960004854 viral vaccine Drugs 0.000 description 3
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 102000014150 Interferons Human genes 0.000 description 2
- 108010050904 Interferons Proteins 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 125000003277 amino group Chemical group 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 229940047124 interferons Drugs 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000002906 microbiologic effect Effects 0.000 description 2
- 239000011541 reaction mixture Substances 0.000 description 2
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000035899 viability Effects 0.000 description 2
- QLOKJRIVRGCVIM-UHFFFAOYSA-N 1-[(4-methylsulfanylphenyl)methyl]piperazine Chemical compound C1=CC(SC)=CC=C1CN1CCNCC1 QLOKJRIVRGCVIM-UHFFFAOYSA-N 0.000 description 1
- YMDNODNLFSHHCV-UHFFFAOYSA-N 2-chloro-n,n-diethylethanamine Chemical compound CCN(CC)CCCl YMDNODNLFSHHCV-UHFFFAOYSA-N 0.000 description 1
- 206010059837 Adhesion Diseases 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 description 1
- CPELXLSAUQHCOX-UHFFFAOYSA-M Bromide Chemical compound [Br-] CPELXLSAUQHCOX-UHFFFAOYSA-M 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 102000008946 Fibrinogen Human genes 0.000 description 1
- 108010049003 Fibrinogen Proteins 0.000 description 1
- 108010009736 Protein Hydrolysates Proteins 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 238000010908 decantation Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000002270 dispersing agent Substances 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 229940012952 fibrinogen Drugs 0.000 description 1
- 239000012847 fine chemical Substances 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 210000005265 lung cell Anatomy 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000004264 monolayer culture Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 229940070376 protein Drugs 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 229910001961 silver nitrate Inorganic materials 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
- 239000011534 wash buffer Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
- C12N5/0075—General culture methods using substrates using microcarriers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/54—Collagen; Gelatin
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicinal Preparation (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR7929910A FR2470794A1 (fr) | 1979-12-05 | 1979-12-05 | Nouvelles microparticules, leur preparation et leurs applications en biologie, notamment a la culture de cellules diploides humaines |
| FR7929910 | 1979-12-05 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1167398A true CA1167398A (en) | 1984-05-15 |
Family
ID=9232425
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000366268A Expired CA1167398A (en) | 1979-12-05 | 1980-12-05 | Microparticles, preparation thereof and applications thereof in biology, particularly in the culture of human diploid cells |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US4373027A (OSRAM) |
| EP (1) | EP0030885B1 (OSRAM) |
| JP (1) | JPS5695196A (OSRAM) |
| CA (1) | CA1167398A (OSRAM) |
| DE (1) | DE3069426D1 (OSRAM) |
| DK (1) | DK518680A (OSRAM) |
| FR (1) | FR2470794A1 (OSRAM) |
Families Citing this family (40)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE456164B (sv) * | 1980-08-20 | 1988-09-12 | Kjell Nilsson | Forfarande for immobilisering, odling och efterfoljande frigoring av animala celler samt mikroberare av gelatin med absorberade animala celler |
| US5489261A (en) * | 1981-01-26 | 1996-02-06 | Trustees Of Boston University | Hydrogels capable of supporting cell growth |
| US5106743A (en) * | 1981-01-26 | 1992-04-21 | Trustees Of Boston University | Hydrogels capable of supporting cell growth |
| DK37083A (da) * | 1982-02-01 | 1983-08-02 | Cancer Res Nat Found | Graensefladedyrkning af celler i vaevskultur |
| EP0101724A1 (en) * | 1982-02-23 | 1984-03-07 | Ventrex Laboratories Inc. | Multipurpose supports for immunological and biological use |
| EP0097907A3 (en) * | 1982-06-25 | 1985-01-09 | Flow General, Inc. | Cell culture microcarriers |
| GB2133308A (en) * | 1983-01-14 | 1984-07-25 | Central Blood Lab Authority | Affinity chromatography absorbent |
| JPS59134732A (ja) * | 1983-01-21 | 1984-08-02 | Green Cross Corp:The | フイブロネクチン・生理活性物質複合体の製造法 |
| JPS59164723A (ja) * | 1983-03-10 | 1984-09-17 | Koken:Kk | 再生コラーゲンフイブリルを含有する基質及びその製造方法 |
| US5387522A (en) * | 1984-06-01 | 1995-02-07 | Schering Corporation | Apparatus having a biphasic spray head for entrapping biological material in a hydrophilic gel |
| EP0217917B1 (en) * | 1985-04-04 | 1993-12-15 | Verax Corporation | Weighted microsponge for immobilizing bioactive material |
| US4861714A (en) * | 1985-04-04 | 1989-08-29 | Verax Corporation | Weighted collagen microsponge for immobilizing bioactive material |
| US5100783A (en) * | 1985-05-10 | 1992-03-31 | Verax Corporation | Weighted microsponge for immobilizing bioactive material |
| EP0233899A1 (en) * | 1985-06-24 | 1987-09-02 | Karyon Technology, Incorporated | Entrapment of anchorage-dependent cells |
| DD274336A3 (de) * | 1985-10-03 | 1989-12-20 | Npo Biolar | Verfahren zur Gewinnung einer Mikroträgersubstanz für die Zellkultivierung |
| SE464816B (sv) * | 1985-10-15 | 1991-06-17 | Nilsson Kjell | Makroporoesa partiklar, foerfarande foer dess framstaellning och dess anvaendning |
| US4919659A (en) * | 1985-12-16 | 1990-04-24 | The Board Of Regents For The University Of Washington | Radio frequency plasma deposited polymers that enhance cell growth |
| AU592670B2 (en) * | 1986-08-15 | 1990-01-18 | Commonwealth Scientific And Industrial Research Organisation | Promoting cell adhesion and growth on a substrate |
| EP0277993A4 (en) * | 1986-08-15 | 1989-01-19 | Commw Scient Ind Res Org | PROMOTION OF CELL ADHESION AND GROWTH ON A SUBSTRATE. |
| US4994388A (en) * | 1988-04-15 | 1991-02-19 | Solohill Engineering, Inc. | Collagen-coated polystyrene microcarrier beads |
| WO1989010397A1 (fr) * | 1988-04-18 | 1989-11-02 | Nitta Gelatin Inc. | Procede pour la mise en culture de cellules animales a grande echelle, et procede pour la preparation du substrat de support a cet effet |
| AU2946295A (en) * | 1994-06-27 | 1996-01-19 | Johns Hopkins University, The | Targeted gene delivery system |
| US6150505A (en) * | 1997-09-19 | 2000-11-21 | Hadasit Medical Research Services & Development Ltd. | Fibrin microbeads prepared from fibrinogen, thrombin and factor XIII |
| US6696089B2 (en) | 1998-09-03 | 2004-02-24 | Board Of Regents Of The University Of Nebraska | Nanogel networks including polyion polymer fragments and biological agent compositions thereof |
| CA2360740A1 (en) | 1999-03-02 | 2000-09-08 | Boehringer Ingelheim Pharmaceuticals, Inc. | Compounds useful as reversible inhibitors of cathepsin s |
| US6552172B2 (en) * | 2001-08-30 | 2003-04-22 | Habto Biotech, Inc. | Fibrin nanoparticles and uses thereof |
| FR2839260B1 (fr) * | 2002-05-03 | 2005-02-25 | Inst Nat Sante Rech Med | Microparticules a base d'un materiau bicompatible et biodegradable, supportant des cellules et des substances biologiquement actives |
| US20050129776A1 (en) | 2002-05-03 | 2005-06-16 | Inserm | Microparticles supporting cells and active substances |
| US7586888B2 (en) * | 2005-02-17 | 2009-09-08 | Mobitrum Corporation | Method and system for mesh network embedded devices |
| US7630736B2 (en) * | 2005-10-11 | 2009-12-08 | Mobitrum Corporation | Method and system for spatial data input, manipulation and distribution via an adaptive wireless transceiver |
| US8427979B1 (en) | 2006-07-27 | 2013-04-23 | Mobitrum Corporation | Method and system for dynamic information exchange on location aware mesh network devices |
| US7801058B2 (en) * | 2006-07-27 | 2010-09-21 | Mobitrum Corporation | Method and system for dynamic information exchange on mesh network devices |
| USRE47894E1 (en) | 2006-07-27 | 2020-03-03 | Iii Holdings 2, Llc | Method and system for dynamic information exchange on location aware mesh network devices |
| US8305935B2 (en) * | 2006-07-27 | 2012-11-06 | Mobitrum Corporation | Method and system for dynamic information exchange on location aware mesh network devices |
| US8411590B2 (en) | 2006-07-27 | 2013-04-02 | Mobitrum Corporation | Mesh network remote control device |
| US8305936B2 (en) | 2006-07-27 | 2012-11-06 | Mobitrum Corporation | Method and system for dynamic information exchange on a mesh network in a vehicle |
| KR100871652B1 (ko) * | 2007-07-30 | 2008-12-02 | 한양대학교 산학협력단 | 세포 배양용 3차원 마이크로 캐리어, 이의 제조방법 및이를 이용한 세포의 3차원 부유 배양법 |
| US20090189739A1 (en) * | 2008-01-25 | 2009-07-30 | Mobitrum Corporation | Passive voice enabled rfid devices |
| CN113249311B (zh) * | 2020-05-25 | 2022-06-28 | 北京唐颐惠康生物医学技术有限公司 | 间充质干细胞培养用可降解微载体的制备方法及其产品和应用 |
| CN114159554B (zh) * | 2021-11-22 | 2022-12-13 | 广州优理氏生物科技有限公司 | 一种纤连蛋白-聚乙烯醇微球的制备方法及其应用 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BE789195A (fr) * | 1971-09-24 | 1973-03-22 | Gist Brocades Nv | Compositions d'enzymes |
| US4036693A (en) * | 1976-02-02 | 1977-07-19 | Massachusetts Institute Of Technology | Treatment of cell culture microcarries |
| ZA776251B (en) * | 1976-11-11 | 1978-07-26 | Massachusetts Inst Technology | Improved cell culture microcarriers |
| US4189534A (en) * | 1976-11-11 | 1980-02-19 | Massachusetts Institute Of Technology | Cell culture microcarriers |
| US4293654A (en) * | 1977-10-17 | 1981-10-06 | Massachusetts Institute Of Technology | Cell culture microcarriers |
| FR2419321A1 (fr) * | 1978-03-10 | 1979-10-05 | Merck & Co Inc | Pretraitement de microsupports pour cultures cellulaires |
| US4266032A (en) * | 1979-08-10 | 1981-05-05 | Monsanto Company | Method of culturing microcarrier supported cells |
| SE445116B (sv) * | 1979-09-12 | 1986-06-02 | Pharmacia Fine Chemicals Ab | Sett att odla celler pa mikroberare med fibronektinytskikt |
-
1979
- 1979-12-05 FR FR7929910A patent/FR2470794A1/fr active Granted
-
1980
- 1980-12-04 DE DE8080401734T patent/DE3069426D1/de not_active Expired
- 1980-12-04 DK DK518680A patent/DK518680A/da not_active Application Discontinuation
- 1980-12-04 JP JP17145680A patent/JPS5695196A/ja active Pending
- 1980-12-04 EP EP80401734A patent/EP0030885B1/fr not_active Expired
- 1980-12-05 CA CA000366268A patent/CA1167398A/en not_active Expired
- 1980-12-05 US US06/213,314 patent/US4373027A/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| FR2470794A1 (fr) | 1981-06-12 |
| EP0030885A3 (en) | 1981-07-15 |
| FR2470794B1 (OSRAM) | 1983-08-19 |
| DE3069426D1 (en) | 1984-11-15 |
| DK518680A (da) | 1981-06-06 |
| US4373027A (en) | 1983-02-08 |
| EP0030885A2 (fr) | 1981-06-24 |
| EP0030885B1 (fr) | 1984-10-10 |
| JPS5695196A (en) | 1981-08-01 |
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