CA1110165A - Double antibody immunoassay using enzyme-labelled antibody and solid phase antigen - Google Patents

Double antibody immunoassay using enzyme-labelled antibody and solid phase antigen

Info

Publication number
CA1110165A
CA1110165A CA292,743A CA292743A CA1110165A CA 1110165 A CA1110165 A CA 1110165A CA 292743 A CA292743 A CA 292743A CA 1110165 A CA1110165 A CA 1110165A
Authority
CA
Canada
Prior art keywords
antibody
antigen
enzyme
sample
insolubilized
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
CA292,743A
Other languages
English (en)
French (fr)
Inventor
Vincent Marks
James W. Bridges
David Morris
Michael J. O'sullivan
Ernesto Gnemmi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pfizer Italia SRL
Original Assignee
Farmitalia Carlo Erba SRL
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Farmitalia Carlo Erba SRL filed Critical Farmitalia Carlo Erba SRL
Application granted granted Critical
Publication of CA1110165A publication Critical patent/CA1110165A/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
CA292,743A 1976-12-10 1977-12-09 Double antibody immunoassay using enzyme-labelled antibody and solid phase antigen Expired CA1110165A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB51709/76 1976-12-10
GB51709/76A GB1549069A (en) 1976-12-10 1976-12-10 Enzyme linked immunoassay

Publications (1)

Publication Number Publication Date
CA1110165A true CA1110165A (en) 1981-10-06

Family

ID=10461077

Family Applications (1)

Application Number Title Priority Date Filing Date
CA292,743A Expired CA1110165A (en) 1976-12-10 1977-12-09 Double antibody immunoassay using enzyme-labelled antibody and solid phase antigen

Country Status (26)

Country Link
JP (1) JPS53101522A (es)
AR (1) AR226805A1 (es)
AU (1) AU517266B2 (es)
BE (1) BE861711A (es)
BR (1) BR7708181A (es)
CA (1) CA1110165A (es)
CH (1) CH628739A5 (es)
CS (1) CS208739B2 (es)
DE (1) DE2755008A1 (es)
DK (1) DK550077A (es)
ES (2) ES464929A1 (es)
FI (1) FI773718A (es)
FR (1) FR2373795A1 (es)
GB (1) GB1549069A (es)
GR (1) GR63580B (es)
HK (1) HK13881A (es)
HU (1) HU179956B (es)
IL (1) IL53576A (es)
IT (1) IT1109485B (es)
MY (1) MY8100367A (es)
NL (1) NL7713692A (es)
NO (1) NO774240L (es)
NZ (1) NZ185927A (es)
PT (1) PT67384B (es)
SE (1) SE7714034L (es)
TR (1) TR20650A (es)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS576363A (en) * 1980-06-13 1982-01-13 Takeda Chem Ind Ltd Production of specific antibody
CA1160566A (en) * 1980-04-25 1984-01-17 Harald Gallati Immunological determination method
ES8307897A1 (es) * 1981-04-13 1983-08-01 Hoechst Co American Un metodo de determinar la presencia de un antigeno en un medio liquido sospechoso de contenerlo.
WO1983000505A1 (en) * 1981-07-31 1983-02-17 Bucher, Doris, J. Assay for viruses
DE3263249D1 (en) * 1981-08-21 1985-05-30 Hoffmann La Roche Method for the determination of carcinoembryonic antigen (cea) and suitable antibody solution for the determination
JPS58151559A (ja) * 1982-03-05 1983-09-08 Takeda Chem Ind Ltd ヒト絨毛性ゴナドトロピンの免疫化学的測定法
US4704356A (en) * 1984-03-27 1987-11-03 Rush-Presbyterian-St. Luke's Medical Center Method of diagnosing cartilage tissue abnormalities
US4734362A (en) * 1986-02-03 1988-03-29 Cambridge Bioscience Corporation Process for purifying recombinant proteins, and products thereof
CH670709A5 (es) * 1986-04-24 1989-06-30 Univ Moskovsk
AT388618B (de) * 1987-05-05 1989-08-10 Binder Bernd Dr Verfahren zur quantitativen bestimmung von funktion und antigener konzentration einer in einer biologischen fluessigkeit enthaltenen substanz
DE3807440A1 (de) * 1988-03-07 1989-09-21 Progen Biotechnik Gmbh Verfahren zum immunologischen nachweis von substanzen sowie eine derartige zusammensetzung und ein testkit
DE19508264C1 (de) * 1995-03-08 1996-02-01 Klose Werner Dipl Ing Fh Verfahren und Vorrichtung zum Vermessen von Konturen, insbesondere des Straßenverlaufs
US6808902B1 (en) * 1999-11-12 2004-10-26 Amgen Inc. Process for correction of a disulfide misfold in IL-1Ra Fc fusion molecules

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL154600B (nl) * 1971-02-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen.
NL154599B (nl) * 1970-12-28 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen, alsmede testverpakking.
US4002532A (en) * 1974-10-21 1977-01-11 Weltman Joel K Enzyme conjugates
US3951748A (en) * 1974-11-11 1976-04-20 Medical Products, Inc. Sensitized matrix for detection of disease
DK140815B (da) * 1975-06-10 1979-11-19 Weeke Bengt Fremgangsmåde til påvisning eller bestemmelse af antistoffer i legemsvæsker ved hjælp af kendte antigener eller til bestemmelse af antigener ved hjælp af kendte antistoffer fra legemsvæsker samt et middel til anvendelse ved udøvelse af fremgangsmåden.

Also Published As

Publication number Publication date
FI773718A (fi) 1978-06-11
NZ185927A (en) 1980-02-21
NO774240L (no) 1978-06-13
CS208739B2 (en) 1981-09-15
PT67384A (en) 1978-01-01
SE7714034L (sv) 1978-06-11
TR20650A (tr) 1982-03-24
HK13881A (en) 1981-04-16
AU517266B2 (en) 1981-07-16
DE2755008A1 (de) 1978-06-15
PT67384B (en) 1979-05-18
GR63580B (en) 1979-11-22
ES464929A1 (es) 1979-01-01
DK550077A (da) 1978-06-11
GB1549069A (en) 1979-08-01
JPS53101522A (en) 1978-09-05
HU179956B (en) 1983-01-28
IL53576A0 (en) 1978-03-10
IT1109485B (it) 1985-12-16
IL53576A (en) 1981-07-31
AR226805A1 (es) 1982-08-31
BR7708181A (pt) 1978-07-11
ES468934A1 (es) 1978-12-16
NL7713692A (nl) 1978-06-13
MY8100367A (en) 1981-12-31
CH628739A5 (en) 1982-03-15
FR2373795A1 (fr) 1978-07-07
FR2373795B1 (es) 1982-09-10
AU3139577A (en) 1979-06-14
BE861711A (fr) 1978-03-31

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