ES464929A1 - Metodo mejorado de ensayo inmunologico de enlace con enzima para la deteccion y determinacion de antigenos - Google Patents
Metodo mejorado de ensayo inmunologico de enlace con enzima para la deteccion y determinacion de antigenosInfo
- Publication number
- ES464929A1 ES464929A1 ES464929A ES464929A ES464929A1 ES 464929 A1 ES464929 A1 ES 464929A1 ES 464929 A ES464929 A ES 464929A ES 464929 A ES464929 A ES 464929A ES 464929 A1 ES464929 A1 ES 464929A1
- Authority
- ES
- Spain
- Prior art keywords
- antigen
- enzyme linked
- linked immunoassay
- insoluble
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
Método mejorado de ensayo inmunológico de enlace con enzima para la detección y determinación de antígenos, que comprende poner en contacto en un medio líquido, simultáneamente o en etapas, (1) un antígeno (según se ha definido en la memoria) sobre un soporte insoluble, o en forma de un agregado insoluble, (2) un primer anticuerpo (según se ha definido en la memoria), y (3) un segundo anticuerpo marcado con enzima (según se ha definido en la memoria); separar los materiales insolubles y solubles; y determinar la actividad de enzima, ya sea en los materiales separados solubles o en los insolubles; caracterizado porque dicho primer anticuerpo (2) se pone también en contacto con una muestra que contiene el antígeno a detectar o determinar, que puede ser el mismo que o diferente del antígeno (1), quedando así el segundo anticuerpo (3) unido al material insoluble en relación inversa a la cantidad de antígeno en la muestra a detectar o determinar, determinando así la presencia o cantidad de antígeno en la muestra.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB51709/76A GB1549069A (en) | 1976-12-10 | 1976-12-10 | Enzyme linked immunoassay |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES464929A1 true ES464929A1 (es) | 1979-01-01 |
Family
ID=10461077
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES464929A Expired ES464929A1 (es) | 1976-12-10 | 1977-12-09 | Metodo mejorado de ensayo inmunologico de enlace con enzima para la deteccion y determinacion de antigenos |
| ES468934A Expired ES468934A1 (es) | 1976-12-10 | 1978-04-19 | Una unidad de ensayo para realizar un metodo de inmunoensayoque emplea enzimas. |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES468934A Expired ES468934A1 (es) | 1976-12-10 | 1978-04-19 | Una unidad de ensayo para realizar un metodo de inmunoensayoque emplea enzimas. |
Country Status (26)
| Country | Link |
|---|---|
| JP (1) | JPS53101522A (es) |
| AR (1) | AR226805A1 (es) |
| AU (1) | AU517266B2 (es) |
| BE (1) | BE861711A (es) |
| BR (1) | BR7708181A (es) |
| CA (1) | CA1110165A (es) |
| CH (1) | CH628739A5 (es) |
| CS (1) | CS208739B2 (es) |
| DE (1) | DE2755008A1 (es) |
| DK (1) | DK550077A (es) |
| ES (2) | ES464929A1 (es) |
| FI (1) | FI773718A (es) |
| FR (1) | FR2373795A1 (es) |
| GB (1) | GB1549069A (es) |
| GR (1) | GR63580B (es) |
| HK (1) | HK13881A (es) |
| HU (1) | HU179956B (es) |
| IL (1) | IL53576A (es) |
| IT (1) | IT1109485B (es) |
| MY (1) | MY8100367A (es) |
| NL (1) | NL7713692A (es) |
| NO (1) | NO774240L (es) |
| NZ (1) | NZ185927A (es) |
| PT (1) | PT67384B (es) |
| SE (1) | SE7714034L (es) |
| TR (1) | TR20650A (es) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS576363A (en) * | 1980-06-13 | 1982-01-13 | Takeda Chem Ind Ltd | Production of specific antibody |
| CA1160566A (en) * | 1980-04-25 | 1984-01-17 | Harald Gallati | Immunological determination method |
| ES511156A0 (es) * | 1981-04-13 | 1983-08-01 | Hoechst Co American | Un metodo de determinar la presencia de un antigeno en un medio liquido sospechoso de contenerlo. |
| DE3176206D1 (en) * | 1981-07-31 | 1987-06-25 | Sinai School Medicine | Assay for viruses |
| EP0072902B1 (de) * | 1981-08-21 | 1985-04-24 | F. HOFFMANN-LA ROCHE & CO. Aktiengesellschaft | Verfahren zur Bestimmung von carcinoembryonalem Antigen (CEA) und zur Bestimmung geeignete Antikörperlösung |
| JPS58151559A (ja) * | 1982-03-05 | 1983-09-08 | Takeda Chem Ind Ltd | ヒト絨毛性ゴナドトロピンの免疫化学的測定法 |
| US4704356A (en) * | 1984-03-27 | 1987-11-03 | Rush-Presbyterian-St. Luke's Medical Center | Method of diagnosing cartilage tissue abnormalities |
| US4734362A (en) * | 1986-02-03 | 1988-03-29 | Cambridge Bioscience Corporation | Process for purifying recombinant proteins, and products thereof |
| CH670709A5 (es) * | 1986-04-24 | 1989-06-30 | Univ Moskovsk | |
| AT388618B (de) * | 1987-05-05 | 1989-08-10 | Binder Bernd Dr | Verfahren zur quantitativen bestimmung von funktion und antigener konzentration einer in einer biologischen fluessigkeit enthaltenen substanz |
| DE3807440A1 (de) * | 1988-03-07 | 1989-09-21 | Progen Biotechnik Gmbh | Verfahren zum immunologischen nachweis von substanzen sowie eine derartige zusammensetzung und ein testkit |
| DE19508264C1 (de) * | 1995-03-08 | 1996-02-01 | Klose Werner Dipl Ing Fh | Verfahren und Vorrichtung zum Vermessen von Konturen, insbesondere des Straßenverlaufs |
| US6808902B1 (en) * | 1999-11-12 | 2004-10-26 | Amgen Inc. | Process for correction of a disulfide misfold in IL-1Ra Fc fusion molecules |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL154600B (nl) * | 1971-02-10 | 1977-09-15 | Organon Nv | Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen. |
| NL154599B (nl) * | 1970-12-28 | 1977-09-15 | Organon Nv | Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen, alsmede testverpakking. |
| US4002532A (en) * | 1974-10-21 | 1977-01-11 | Weltman Joel K | Enzyme conjugates |
| US3951748A (en) * | 1974-11-11 | 1976-04-20 | Medical Products, Inc. | Sensitized matrix for detection of disease |
| DK140815B (da) * | 1975-06-10 | 1979-11-19 | Weeke Bengt | Fremgangsmåde til påvisning eller bestemmelse af antistoffer i legemsvæsker ved hjælp af kendte antigener eller til bestemmelse af antigener ved hjælp af kendte antistoffer fra legemsvæsker samt et middel til anvendelse ved udøvelse af fremgangsmåden. |
-
1976
- 1976-12-10 GB GB51709/76A patent/GB1549069A/en not_active Expired
-
1977
- 1977-12-09 NZ NZ185927A patent/NZ185927A/xx unknown
- 1977-12-09 DK DK550077A patent/DK550077A/da not_active Application Discontinuation
- 1977-12-09 CA CA292,743A patent/CA1110165A/en not_active Expired
- 1977-12-09 HU HU77EA179A patent/HU179956B/hu unknown
- 1977-12-09 NL NL7713692A patent/NL7713692A/xx not_active Application Discontinuation
- 1977-12-09 JP JP14733977A patent/JPS53101522A/ja active Pending
- 1977-12-09 FI FI773718A patent/FI773718A/fi not_active Application Discontinuation
- 1977-12-09 AR AR270301A patent/AR226805A1/es active
- 1977-12-09 BR BR7708181A patent/BR7708181A/pt unknown
- 1977-12-09 ES ES464929A patent/ES464929A1/es not_active Expired
- 1977-12-09 NO NO774240A patent/NO774240L/no unknown
- 1977-12-09 IL IL53576A patent/IL53576A/xx unknown
- 1977-12-09 CH CH1516677A patent/CH628739A5/fr not_active IP Right Cessation
- 1977-12-09 PT PT67384A patent/PT67384B/pt unknown
- 1977-12-09 DE DE19772755008 patent/DE2755008A1/de not_active Ceased
- 1977-12-09 FR FR7737194A patent/FR2373795A1/fr active Granted
- 1977-12-09 GR GR54943A patent/GR63580B/el unknown
- 1977-12-09 TR TR20650A patent/TR20650A/xx unknown
- 1977-12-09 SE SE7714034A patent/SE7714034L/ not_active Application Discontinuation
- 1977-12-09 BE BE183341A patent/BE861711A/xx not_active IP Right Cessation
- 1977-12-09 AU AU31395/77A patent/AU517266B2/en not_active Expired
- 1977-12-09 IT IT30554/77A patent/IT1109485B/it active
- 1977-12-09 CS CS778265A patent/CS208739B2/cs unknown
-
1978
- 1978-04-19 ES ES468934A patent/ES468934A1/es not_active Expired
-
1981
- 1981-04-09 HK HK138/81A patent/HK13881A/xx unknown
- 1981-12-30 MY MY367/81A patent/MY8100367A/xx unknown
Also Published As
| Publication number | Publication date |
|---|---|
| IL53576A (en) | 1981-07-31 |
| IT1109485B (it) | 1985-12-16 |
| BE861711A (fr) | 1978-03-31 |
| IL53576A0 (en) | 1978-03-10 |
| DE2755008A1 (de) | 1978-06-15 |
| BR7708181A (pt) | 1978-07-11 |
| GB1549069A (en) | 1979-08-01 |
| AR226805A1 (es) | 1982-08-31 |
| JPS53101522A (en) | 1978-09-05 |
| FI773718A (fi) | 1978-06-11 |
| GR63580B (en) | 1979-11-22 |
| CA1110165A (en) | 1981-10-06 |
| HU179956B (en) | 1983-01-28 |
| NO774240L (no) | 1978-06-13 |
| AU3139577A (en) | 1979-06-14 |
| PT67384A (en) | 1978-01-01 |
| FR2373795A1 (fr) | 1978-07-07 |
| CH628739A5 (en) | 1982-03-15 |
| AU517266B2 (en) | 1981-07-16 |
| FR2373795B1 (es) | 1982-09-10 |
| SE7714034L (sv) | 1978-06-11 |
| MY8100367A (en) | 1981-12-31 |
| NL7713692A (nl) | 1978-06-13 |
| PT67384B (en) | 1979-05-18 |
| HK13881A (en) | 1981-04-16 |
| DK550077A (da) | 1978-06-11 |
| TR20650A (tr) | 1982-03-24 |
| NZ185927A (en) | 1980-02-21 |
| CS208739B2 (en) | 1981-09-15 |
| ES468934A1 (es) | 1978-12-16 |
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