BRPI0817212A2 - Pirrolisil-trna sintetase mutante, e método para a produção de proteína tendo aminoácido não-natural integrado nela por sua utilização - Google Patents
Pirrolisil-trna sintetase mutante, e método para a produção de proteína tendo aminoácido não-natural integrado nela por sua utilizaçãoInfo
- Publication number
- BRPI0817212A2 BRPI0817212A2 BRPI0817212-9A2A BRPI0817212A BRPI0817212A2 BR PI0817212 A2 BRPI0817212 A2 BR PI0817212A2 BR PI0817212 A BRPI0817212 A BR PI0817212A BR PI0817212 A2 BRPI0817212 A2 BR PI0817212A2
- Authority
- BR
- Brazil
- Prior art keywords
- pirrolisil
- syntetase
- amino acid
- natural amino
- protein production
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2007243574 | 2007-09-20 | ||
PCT/JP2008/067029 WO2009038195A1 (ja) | 2007-09-20 | 2008-09-19 | 変異体ピロリジル-tRNA合成酵素及びこれを用いる非天然アミノ酸組み込みタンパク質の製造方法 |
Publications (1)
Publication Number | Publication Date |
---|---|
BRPI0817212A2 true BRPI0817212A2 (pt) | 2014-10-14 |
Family
ID=40468004
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BRPI0817212-9A2A BRPI0817212A2 (pt) | 2007-09-20 | 2008-09-19 | Pirrolisil-trna sintetase mutante, e método para a produção de proteína tendo aminoácido não-natural integrado nela por sua utilização |
Country Status (8)
Country | Link |
---|---|
US (2) | US8735093B2 (pt) |
EP (1) | EP2192185B1 (pt) |
JP (1) | JP5590649B2 (pt) |
CN (1) | CN101952428B (pt) |
AU (1) | AU2008301614A1 (pt) |
BR (1) | BRPI0817212A2 (pt) |
CA (1) | CA2706889C (pt) |
WO (1) | WO2009038195A1 (pt) |
Families Citing this family (42)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BRPI0817212A2 (pt) | 2007-09-20 | 2014-10-14 | Riken | Pirrolisil-trna sintetase mutante, e método para a produção de proteína tendo aminoácido não-natural integrado nela por sua utilização |
JP5419220B2 (ja) * | 2007-11-22 | 2014-02-19 | 独立行政法人理化学研究所 | エステル結合を含む非天然タンパク質の製造方法 |
JP2013522352A (ja) * | 2010-03-24 | 2013-06-13 | メディカル リサーチ カウンシル | 方法 |
EP2670767B1 (en) * | 2011-02-03 | 2017-12-20 | European Molecular Biology Laboratory | Unnatural amino acids comprising a cyclooctynyl or trans-cyclooctenyl analog group and uses thereof |
CN102838663B (zh) * | 2011-06-23 | 2014-06-11 | 北京大学 | 定点突变和定点修饰的病毒膜蛋白、其制备方法及其应用 |
JP2013107842A (ja) * | 2011-11-18 | 2013-06-06 | Institute Of Physical & Chemical Research | 新規な非天然型アミノ酸、及びその利用 |
CN102504022A (zh) * | 2011-11-30 | 2012-06-20 | 苏州元基生物技术有限公司 | 含有保护赖氨酸的胰岛素原及使用其制备胰岛素的方法 |
BR112014028588B1 (pt) * | 2012-05-18 | 2023-03-21 | United Kingdom Research And Innovation | Polipeptídeo, método de produzir um polipeptídeo que compreende o grupo bcn, método de produzir um polipeptídeo que compreende um grupo tetrazina, aminoácido e bcn lisina |
JP6321658B2 (ja) | 2012-09-24 | 2018-05-09 | メディミューン リミテッド | アミノ酸誘導体 |
SG11201507104WA (en) * | 2013-03-15 | 2015-10-29 | Medimmune Ltd | Novel nucleic acid molecules |
WO2015107064A1 (en) | 2014-01-14 | 2015-07-23 | European Molecular Biology Laboratory | Multiple cycloaddition reactions for labeling of molecules |
GB2528227A (en) | 2014-03-14 | 2016-01-20 | Medical Res Council | Cyclopropene amino acids and methods |
EP3310924A1 (en) * | 2014-06-17 | 2018-04-25 | B.G. Negev Technologies and Applications Ltd., at Ben-Gurion University | Genetically expanded cell free protein synthesis systems, methods and kits |
GB201419109D0 (en) | 2014-10-27 | 2014-12-10 | Medical Res Council | Incorporation of unnatural amino acids into proteins |
US10815489B2 (en) * | 2015-03-13 | 2020-10-27 | Chugai Seiyaku Kabushiki Kaisha | Modified aminoacyl-tRNA synthetase and use thereof |
JP6967221B2 (ja) * | 2015-08-19 | 2021-11-17 | 国立研究開発法人理化学研究所 | 非天然アミノ酸導入抗体 |
CN105541962B (zh) * | 2015-12-19 | 2021-04-02 | 深圳市贝沃德克生物技术研究院有限公司 | 基于7-羟基香豆素的定点荧光标记方法 |
CN105566440B (zh) * | 2016-01-22 | 2021-04-02 | 深圳市贝沃德克生物技术研究院有限公司 | 基于nbdk的定点荧光标记方法 |
WO2017161183A1 (en) | 2016-03-16 | 2017-09-21 | The Regents Of The University Of California | Covalent peptide binders |
WO2018044143A1 (ko) * | 2016-09-05 | 2018-03-08 | 한국과학기술원 | 목적 단백질의 위치 특이적 변형이 시공간적으로 조절되는 마우스, 이의 제조방법 및 그 용도 |
EP3309260A1 (en) | 2016-10-14 | 2018-04-18 | European Molecular Biology Laboratory | Archaeal pyrrolysyl trna synthetases for orthogonal use |
TWI821192B (zh) | 2017-07-11 | 2023-11-11 | 美商新索思股份有限公司 | 非天然核苷酸之導入及其方法 |
CN109295100A (zh) * | 2017-07-25 | 2019-02-01 | 北京大学 | 携带正交tRNA/氨酰tRNA合成酶的稳定细胞系的构建 |
CN108588040B (zh) * | 2018-06-08 | 2021-06-15 | 中国医学科学院病原生物学研究所 | 重组MtMetRS、其晶体及它们在制备抗结核药物中的应用 |
US20210324363A1 (en) * | 2018-08-31 | 2021-10-21 | Riken | Pyrrolysyl-trna synthetase |
EP3696189A1 (en) | 2019-02-14 | 2020-08-19 | European Molecular Biology Laboratory | Means and methods for preparing engineered target proteins by genetic code expansion in a target protein selective manner |
CN111718920B (zh) * | 2019-03-19 | 2021-05-07 | 宁波鲲鹏生物科技有限公司 | 在蛋白中高效引入赖氨酸衍生物的氨酰基-tRNA合成酶 |
CN111718949B (zh) * | 2019-03-19 | 2021-10-01 | 宁波鲲鹏生物科技有限公司 | 利用双质粒系统在蛋白中引入非天然氨基酸 |
CN111849929B (zh) * | 2019-04-30 | 2021-05-11 | 苏州鲲鹏生物技术有限公司 | 高效引入赖氨酸衍生物的氨酰基—tRNA合成酶 |
WO2020257668A1 (en) * | 2019-06-21 | 2020-12-24 | Trustees Of Boston College | Enhanced platforms for unnatural amino acid incorporation in mammalian cells |
EP3868882A1 (en) | 2020-02-21 | 2021-08-25 | European Molecular Biology Laboratory | Archaeal pyrrolysyl trna synthetases for orthogonal use |
JP7521804B2 (ja) | 2020-03-06 | 2024-07-24 | 国立研究開発法人農業・食品産業技術総合研究機構 | カウンターセレクションマーカー |
CN111747869B (zh) * | 2020-06-12 | 2021-10-22 | 北京大学深圳研究生院 | 一种遗传编码的甲醛反应性非天然氨基酸、制备方法及其应用 |
US20240117295A1 (en) | 2021-05-28 | 2024-04-11 | United Kingdom Research And Innovation | Microorganisms and uses thereof |
GB202110137D0 (en) | 2021-07-14 | 2021-08-25 | Res & Innovation Uk | Methods for optimising protein production |
CN115701451B (zh) * | 2021-08-02 | 2023-08-01 | 宁波鲲鹏生物科技有限公司 | 高效引入赖氨酸衍生物的氨酰基-tRNA合成酶及其应用 |
WO2023031445A2 (en) | 2021-09-06 | 2023-03-09 | Veraxa Biotech Gmbh | Novel aminoacyl-trna synthetase variants for genetic code expansion in eukaryotes |
CN114107394B (zh) * | 2021-11-05 | 2024-01-30 | 中国科学院精密测量科学与技术创新研究院 | 一种慢病毒转移载体、表达PylRS及tRNACUA的细胞系及制备方法与应用 |
AU2022395626A1 (en) | 2021-11-25 | 2024-05-30 | Veraxa Biotech Gmbh | Improved antibody-payload conjugates (apcs) prepared by site-specific conjugation utilizing genetic code expansion |
EP4186529A1 (en) | 2021-11-25 | 2023-05-31 | Veraxa Biotech GmbH | Improved antibody-payload conjugates (apcs) prepared by site-specific conjugation utilizing genetic code expansion |
CN114657156A (zh) * | 2022-03-11 | 2022-06-24 | 南京中医药大学 | 吡咯赖氨酸氨酰-tRNA合成酶突变体及其筛选系统和应用 |
CN118086431A (zh) * | 2024-04-12 | 2024-05-28 | 修实生物医药(如东)有限公司 | 在蛋白质中引入α-羟基酸的方法 |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS625942A (ja) * | 1985-07-02 | 1987-01-12 | Toray Ind Inc | L−またはd−n2−ベンジルオキシカルボニルリジンの製造方法 |
JP2006180701A (ja) | 2003-02-10 | 2006-07-13 | Institute Of Physical & Chemical Research | チロシルtRNA合成酵素の変異体及びその作製方法 |
US20060183888A1 (en) * | 2004-08-13 | 2006-08-17 | Chan Michael K | Pyrrolysine synthesis and modification |
US20060166319A1 (en) * | 2004-08-13 | 2006-07-27 | Chan Michael K | Charging tRNA with pyrrolysine |
JP2007037445A (ja) * | 2005-08-02 | 2007-02-15 | Institute Of Physical & Chemical Research | tRNA合成方法、核酸、アミノアシルtRNA合成方法及び非天然型アミノ酸組み込み蛋白質の製造方法 |
CA2648009A1 (en) | 2006-02-22 | 2007-09-07 | Riken | Method for synthesis of suppressor trna, dna construct, and production of protein having non-natural amino acid integrated therein by using the dna construct |
BRPI0817212A2 (pt) | 2007-09-20 | 2014-10-14 | Riken | Pirrolisil-trna sintetase mutante, e método para a produção de proteína tendo aminoácido não-natural integrado nela por sua utilização |
-
2008
- 2008-09-19 BR BRPI0817212-9A2A patent/BRPI0817212A2/pt not_active IP Right Cessation
- 2008-09-19 AU AU2008301614A patent/AU2008301614A1/en not_active Abandoned
- 2008-09-19 EP EP08832011.4A patent/EP2192185B1/en active Active
- 2008-09-19 JP JP2009533208A patent/JP5590649B2/ja active Active
- 2008-09-19 CA CA2706889A patent/CA2706889C/en active Active
- 2008-09-19 WO PCT/JP2008/067029 patent/WO2009038195A1/ja active Application Filing
- 2008-09-19 CN CN2008801078970A patent/CN101952428B/zh active Active
-
2010
- 2010-03-18 US US12/727,037 patent/US8735093B2/en active Active
-
2014
- 2014-04-08 US US14/248,062 patent/US9133449B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
EP2192185A1 (en) | 2010-06-02 |
CN101952428B (zh) | 2013-10-23 |
EP2192185A4 (en) | 2010-09-22 |
US20140322751A1 (en) | 2014-10-30 |
US8735093B2 (en) | 2014-05-27 |
US20100267087A1 (en) | 2010-10-21 |
JPWO2009038195A1 (ja) | 2011-01-13 |
CA2706889C (en) | 2018-08-28 |
AU2008301614A2 (en) | 2010-05-27 |
US9133449B2 (en) | 2015-09-15 |
EP2192185B1 (en) | 2014-01-22 |
AU2008301614A1 (en) | 2009-03-26 |
CA2706889A1 (en) | 2009-03-26 |
CN101952428A (zh) | 2011-01-19 |
AU2008301614A8 (en) | 2010-06-03 |
WO2009038195A1 (ja) | 2009-03-26 |
JP5590649B2 (ja) | 2014-09-17 |
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Legal Events
Date | Code | Title | Description |
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B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE A 7A ANUIDADE. |
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B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2329 DE 25-08-2015 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013. |
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B350 | Update of information on the portal [chapter 15.35 patent gazette] |