BRPI0610367A2 - uso de um ácido nucleico, método para ligação de um receptor intracelular, composição, composição farmacêutica, ácido nucleico de ligação a hmga, método para seleção de um antagonista ou agonista de hmga, kit para detecção de hmga, antagonista e agonista de hmga e complexo - Google Patents

Info

Publication number

BRPI0610367A2

BRPI0610367A2 BRPI0610367-7A BRPI0610367A BRPI0610367A2 BR PI0610367 A2 BRPI0610367 A2 BR PI0610367A2 BR PI0610367 A BRPI0610367 A BR PI0610367A BR PI0610367 A2 BRPI0610367 A2 BR PI0610367A2

Authority

BR

Brazil

Prior art keywords

helix

section

nucleic acid

hmga

nucleotides

Prior art date

2005-05-04

Links

• Espacenet
• Global Dossier
• Discuss

• 150000007523 nucleic acids Chemical class 0.000 title claims description 363
• 102000039446 nucleic acids Human genes 0.000 title claims description 358
• 108020004707 nucleic acids Proteins 0.000 title claims description 358
• 230000027455 binding Effects 0.000 title claims description 183
• 238000000034 method Methods 0.000 title claims description 62
• 102000027411 intracellular receptors Human genes 0.000 title claims description 53
• 108091008582 intracellular receptors Proteins 0.000 title claims description 53
• 239000000203 mixture Substances 0.000 title claims description 48
• 239000005557 antagonist Substances 0.000 title claims description 29
• 239000000556 agonist Substances 0.000 title claims description 26
• 238000001514 detection method Methods 0.000 title claims description 26
• 239000008194 pharmaceutical composition Substances 0.000 title claims description 14
• 239000002773 nucleotide Substances 0.000 claims description 260
• 125000003729 nucleotide group Chemical group 0.000 claims description 239
• 102000009012 HMGA Proteins Human genes 0.000 claims description 201
• 108010049069 HMGA Proteins Proteins 0.000 claims description 201
• NPOAOTPXWNWTSH-UHFFFAOYSA-N 3-hydroxy-3-methylglutaric acid Chemical group OC(=O)CC(O)(C)CC(O)=O NPOAOTPXWNWTSH-UHFFFAOYSA-N 0.000 claims description 169
• 108091027076 Spiegelmer Proteins 0.000 claims description 139
• 206010028980 Neoplasm Diseases 0.000 claims description 89
• 229920002873 Polyethylenimine Polymers 0.000 claims description 70
• 108090000623 proteins and genes Proteins 0.000 claims description 67
• 239000000693 micelle Substances 0.000 claims description 55
• 102100029009 High mobility group protein HMG-I/HMG-Y Human genes 0.000 claims description 53
• 101000986380 Homo sapiens High mobility group protein HMG-I/HMG-Y Proteins 0.000 claims description 53
• 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 53
• 201000011510 cancer Diseases 0.000 claims description 52
• 201000010099 disease Diseases 0.000 claims description 49
• 230000003834 intracellular effect Effects 0.000 claims description 48
• 102000004169 proteins and genes Human genes 0.000 claims description 42
• 239000003981 vehicle Substances 0.000 claims description 38
• 108020004414 DNA Proteins 0.000 claims description 34
• 102000005962 receptors Human genes 0.000 claims description 34
• 108020003175 receptors Proteins 0.000 claims description 34
• 238000009396 hybridization Methods 0.000 claims description 33
• 108090000765 processed proteins & peptides Proteins 0.000 claims description 31
• 239000000543 intermediate Substances 0.000 claims description 27
• 108700039143 HMGA2 Proteins 0.000 claims description 24
• 102100028999 High mobility group protein HMGI-C Human genes 0.000 claims description 24
• 101150073387 Hmga2 gene Proteins 0.000 claims description 23
• 238000011282 treatment Methods 0.000 claims description 22
• 102000040945 Transcription factor Human genes 0.000 claims description 20
• 108091023040 Transcription factor Proteins 0.000 claims description 20
• 230000015572 biosynthetic process Effects 0.000 claims description 20
• 102000004196 processed proteins & peptides Human genes 0.000 claims description 20
• 239000003814 drug Substances 0.000 claims description 16
• 230000004048 modification Effects 0.000 claims description 16
• 238000012986 modification Methods 0.000 claims description 16
• 229920001184 polypeptide Polymers 0.000 claims description 15
• 150000002632 lipids Chemical class 0.000 claims description 14
• 238000012360 testing method Methods 0.000 claims description 14
• 206010027476 Metastases Diseases 0.000 claims description 12
• 230000001404 mediated effect Effects 0.000 claims description 12
• 206010061289 metastatic neoplasm Diseases 0.000 claims description 12
• 102000004190 Enzymes Human genes 0.000 claims description 11
• 108090000790 Enzymes Proteins 0.000 claims description 11
• 102000018802 High Mobility Group Proteins Human genes 0.000 claims description 11
• 108010052512 High Mobility Group Proteins Proteins 0.000 claims description 11
• 150000001720 carbohydrates Chemical class 0.000 claims description 11
• 235000014633 carbohydrates Nutrition 0.000 claims description 11
• 239000003795 chemical substances by application Substances 0.000 claims description 11
• 230000002503 metabolic effect Effects 0.000 claims description 11
• 210000004072 lung Anatomy 0.000 claims description 10
• 238000011534 incubation Methods 0.000 claims description 9
• 238000004519 manufacturing process Methods 0.000 claims description 9
• 102000052510 DNA-Binding Proteins Human genes 0.000 claims description 8
• 108700020911 DNA-Binding Proteins Proteins 0.000 claims description 8
• 208000016018 endometrial polyp Diseases 0.000 claims description 8
• 201000008806 mesenchymal cell neoplasm Diseases 0.000 claims description 8
• 230000001394 metastastic effect Effects 0.000 claims description 8
• 239000002105 nanoparticle Substances 0.000 claims description 8
• 210000000496 pancreas Anatomy 0.000 claims description 8
• 230000002265 prevention Effects 0.000 claims description 8
• 230000001173 tumoral effect Effects 0.000 claims description 8
• 206010046811 uterine polyp Diseases 0.000 claims description 8
• 206010024612 Lipoma Diseases 0.000 claims description 7
• 108010006519 Molecular Chaperones Proteins 0.000 claims description 7
• 239000002502 liposome Substances 0.000 claims description 7
• 208000026310 Breast neoplasm Diseases 0.000 claims description 6
• 229920000858 Cyclodextrin Polymers 0.000 claims description 6
• 206010025323 Lymphomas Diseases 0.000 claims description 6
• 208000016160 chondroid hamartoma Diseases 0.000 claims description 6
• 210000001072 colon Anatomy 0.000 claims description 6
• 239000000032 diagnostic agent Substances 0.000 claims description 6
• 229940039227 diagnostic agent Drugs 0.000 claims description 6
• 208000032839 leukemia Diseases 0.000 claims description 6
• 208000004333 pleomorphic adenoma Diseases 0.000 claims description 6
• 210000003079 salivary gland Anatomy 0.000 claims description 6
• 210000001685 thyroid gland Anatomy 0.000 claims description 6
• 206010006187 Breast cancer Diseases 0.000 claims description 5
• 208000021994 Diffuse astrocytoma Diseases 0.000 claims description 5
• 208000002966 Giant Cell Tumor of Bone Diseases 0.000 claims description 5
• 208000006050 Hemangiopericytoma Diseases 0.000 claims description 5
• 102000001702 Intracellular Signaling Peptides and Proteins Human genes 0.000 claims description 5
• 108010068964 Intracellular Signaling Peptides and Proteins Proteins 0.000 claims description 5
• 206010058467 Lung neoplasm malignant Diseases 0.000 claims description 5
• 206010029260 Neuroblastoma Diseases 0.000 claims description 5
• 208000000453 Skin Neoplasms Diseases 0.000 claims description 5
• 208000036142 Viral infection Diseases 0.000 claims description 5
• 210000000481 breast Anatomy 0.000 claims description 5
• 208000020719 chondrogenic neoplasm Diseases 0.000 claims description 5
• 229940097362 cyclodextrins Drugs 0.000 claims description 5
• 238000004520 electroporation Methods 0.000 claims description 5
• 201000001169 fibrillary astrocytoma Diseases 0.000 claims description 5
• 208000002154 non-small cell lung carcinoma Diseases 0.000 claims description 5
• 210000001672 ovary Anatomy 0.000 claims description 5
• 239000012071 phase Substances 0.000 claims description 5
• 210000002784 stomach Anatomy 0.000 claims description 5
• 230000009385 viral infection Effects 0.000 claims description 5
• 208000011691 Burkitt lymphomas Diseases 0.000 claims description 4
• 210000003679 cervix uteri Anatomy 0.000 claims description 4
• 239000000412 dendrimer Substances 0.000 claims description 4
• 229920000736 dendritic polymer Polymers 0.000 claims description 4
• 239000003596 drug target Substances 0.000 claims description 4
• 230000000799 fusogenic effect Effects 0.000 claims description 4
• 201000005202 lung cancer Diseases 0.000 claims description 4
• 208000020816 lung neoplasm Diseases 0.000 claims description 4
• 239000011859 microparticle Substances 0.000 claims description 4
• QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 claims description 4
• 210000002307 prostate Anatomy 0.000 claims description 4
• 201000000849 skin cancer Diseases 0.000 claims description 4
• 239000007790 solid phase Substances 0.000 claims description 4
• 230000001960 triggered effect Effects 0.000 claims description 4
• 238000002604 ultrasonography Methods 0.000 claims description 4
• 206010060862 Prostate cancer Diseases 0.000 claims description 3
• 108010076504 Protein Sorting Signals Proteins 0.000 claims description 3
• 208000008443 pancreatic carcinoma Diseases 0.000 claims description 3
• 206010061902 Pancreatic neoplasm Diseases 0.000 claims description 2
• 208000000236 Prostatic Neoplasms Diseases 0.000 claims description 2
• 239000003937 drug carrier Substances 0.000 claims description 2
• 208000021045 exocrine pancreatic carcinoma Diseases 0.000 claims description 2
• 230000007505 plaque formation Effects 0.000 claims description 2
• 201000001514 prostate carcinoma Diseases 0.000 claims description 2
• 230000010837 receptor-mediated endocytosis Effects 0.000 claims description 2
• 208000020615 rectal carcinoma Diseases 0.000 claims description 2
• 201000001320 Atherosclerosis Diseases 0.000 claims 4
• 241000520223 Helice Species 0.000 claims 2
• 208000024770 Thyroid neoplasm Diseases 0.000 claims 2
• 206010008342 Cervix carcinoma Diseases 0.000 claims 1
• 206010009944 Colon cancer Diseases 0.000 claims 1
• 241001077885 Gesta Species 0.000 claims 1
• 208000015634 Rectal Neoplasms Diseases 0.000 claims 1
• 208000005718 Stomach Neoplasms Diseases 0.000 claims 1
• 208000006105 Uterine Cervical Neoplasms Diseases 0.000 claims 1
• 230000003143 atherosclerotic effect Effects 0.000 claims 1
• 208000019065 cervical carcinoma Diseases 0.000 claims 1
• 208000029742 colonic neoplasm Diseases 0.000 claims 1
• 206010017758 gastric cancer Diseases 0.000 claims 1
• 208000010749 gastric carcinoma Diseases 0.000 claims 1
• 201000000498 stomach carcinoma Diseases 0.000 claims 1
• 239000013543 active substance Substances 0.000 abstract description 26
• 210000004027 cell Anatomy 0.000 description 139
• 108091032973 (ribonucleotides)n+m Proteins 0.000 description 54
• 108700039142 HMGA1a Proteins 0.000 description 50
• 102000049983 HMGA1a Human genes 0.000 description 50
• 230000014509 gene expression Effects 0.000 description 43
• 235000018102 proteins Nutrition 0.000 description 39
• 108091023037 Aptamer Proteins 0.000 description 34
• 210000001519 tissue Anatomy 0.000 description 33
• 238000009826 distribution Methods 0.000 description 31
• 241000282414 Homo sapiens Species 0.000 description 29
• 239000000872 buffer Substances 0.000 description 29
• 230000003993 interaction Effects 0.000 description 28
• 230000000694 effects Effects 0.000 description 26
• 150000001875 compounds Chemical class 0.000 description 20
• 229920001223 polyethylene glycol Polymers 0.000 description 20
• 238000003556 assay Methods 0.000 description 19
• 239000000562 conjugate Substances 0.000 description 19
• 210000000170 cell membrane Anatomy 0.000 description 18
• 239000000243 solution Substances 0.000 description 18
• 239000002202 Polyethylene glycol Substances 0.000 description 17
• 239000000126 substance Substances 0.000 description 17
• 241001465754 Metazoa Species 0.000 description 16
• 108091034117 Oligonucleotide Proteins 0.000 description 16
• 101100184710 Xenopus laevis cdc25-1-a gene Proteins 0.000 description 16
• 230000005764 inhibitory process Effects 0.000 description 16
• 238000004904 shortening Methods 0.000 description 15
• 230000004614 tumor growth Effects 0.000 description 15
• 238000013459 approach Methods 0.000 description 14
• 210000002381 plasma Anatomy 0.000 description 14
• 239000000523 sample Substances 0.000 description 14
• 230000032258 transport Effects 0.000 description 14
• 239000002253 acid Substances 0.000 description 13
• 210000000172 cytosol Anatomy 0.000 description 13
• 238000003745 diagnosis Methods 0.000 description 13
• 210000001163 endosome Anatomy 0.000 description 12
• 230000035755 proliferation Effects 0.000 description 12
• JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 11
• 230000000875 corresponding effect Effects 0.000 description 11
• 230000008878 coupling Effects 0.000 description 11
• 238000010168 coupling process Methods 0.000 description 11
• 238000005859 coupling reaction Methods 0.000 description 11
• 239000000499 gel Substances 0.000 description 11
• 238000001727 in vivo Methods 0.000 description 11
• 238000001890 transfection Methods 0.000 description 11
• 241000699670 Mus sp. Species 0.000 description 10
• 229920001213 Polysorbate 20 Polymers 0.000 description 10
• FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 10
• 230000033228 biological regulation Effects 0.000 description 10
• 239000012528 membrane Substances 0.000 description 10
• 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 10
• 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 10
• -1 rRNAs Chemical class 0.000 description 10
• IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 9
• LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 9
• 108010090804 Streptavidin Proteins 0.000 description 9
• 235000001014 amino acid Nutrition 0.000 description 9
• 150000001413 amino acids Chemical class 0.000 description 9
• 238000004458 analytical method Methods 0.000 description 9
• 230000008859 change Effects 0.000 description 9
• 230000006870 function Effects 0.000 description 9
• RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 description 9
• 210000000056 organ Anatomy 0.000 description 9
• 238000013518 transcription Methods 0.000 description 9
• 230000035897 transcription Effects 0.000 description 9
• YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 8
• TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 8
• 101710163270 Nuclease Proteins 0.000 description 8
• 101000595993 Phyllomedusa sauvagei Phylloseptin-S1 Proteins 0.000 description 8
• 230000000692 anti-sense effect Effects 0.000 description 8
• 238000006243 chemical reaction Methods 0.000 description 8
• 230000000295 complement effect Effects 0.000 description 8
• 210000003527 eukaryotic cell Anatomy 0.000 description 8
• 238000002474 experimental method Methods 0.000 description 8
• 238000000338 in vitro Methods 0.000 description 8
• 108020004999 messenger RNA Proteins 0.000 description 8
• PXHVJJICTQNCMI-UHFFFAOYSA-N nickel Substances [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 8
• 229910052757 nitrogen Inorganic materials 0.000 description 8
• 238000000159 protein binding assay Methods 0.000 description 8
• 206010003210 Arteriosclerosis Diseases 0.000 description 7
• 108700020796 Oncogene Proteins 0.000 description 7
• 208000011775 arteriosclerosis disease Diseases 0.000 description 7
• 239000006143 cell culture medium Substances 0.000 description 7
• 230000004663 cell proliferation Effects 0.000 description 7
• 230000001143 conditioned effect Effects 0.000 description 7
• 229940088598 enzyme Drugs 0.000 description 7
• 238000011835 investigation Methods 0.000 description 7
• 230000009401 metastasis Effects 0.000 description 7
• 238000004806 packaging method and process Methods 0.000 description 7
• 230000008569 process Effects 0.000 description 7
• 239000000047 product Substances 0.000 description 7
• 230000002829 reductive effect Effects 0.000 description 7
• 102100032912 CD44 antigen Human genes 0.000 description 6
• KKBWDNZXYLGJEY-UHFFFAOYSA-N Gly-Arg-Pro Natural products NCC(=O)NC(CCNC(=N)N)C(=O)N1CCCC1C(=O)O KKBWDNZXYLGJEY-UHFFFAOYSA-N 0.000 description 6
• 101000868273 Homo sapiens CD44 antigen Proteins 0.000 description 6
• 239000011575 calcium Substances 0.000 description 6
• 238000013461 design Methods 0.000 description 6
• 239000000975 dye Substances 0.000 description 6
• 238000005516 engineering process Methods 0.000 description 6
• 238000009472 formulation Methods 0.000 description 6
• 239000012634 fragment Substances 0.000 description 6
• 108020001507 fusion proteins Proteins 0.000 description 6
• 102000037865 fusion proteins Human genes 0.000 description 6
• 239000011777 magnesium Substances 0.000 description 6
• 210000001236 prokaryotic cell Anatomy 0.000 description 6
• 230000009467 reduction Effects 0.000 description 6
• 230000002441 reversible effect Effects 0.000 description 6
• 238000012546 transfer Methods 0.000 description 6
• HQIZDMIGUJOSNI-IUCAKERBSA-N Arg-Gly-Arg Chemical compound N[C@@H](CCCNC(N)=N)C(=O)NCC(=O)N[C@@H](CCCNC(N)=N)C(O)=O HQIZDMIGUJOSNI-IUCAKERBSA-N 0.000 description 5
• 208000005623 Carcinogenesis Diseases 0.000 description 5
• 102100031181 Glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 5
• 101000936546 Homo sapiens Anion exchange protein 2 Proteins 0.000 description 5
• 108020004711 Nucleic Acid Probes Proteins 0.000 description 5
• 229910019142 PO4 Inorganic materials 0.000 description 5
• PLXBWHJQWKZRKG-UHFFFAOYSA-N Resazurin Chemical compound C1=CC(=O)C=C2OC3=CC(O)=CC=C3[N+]([O-])=C21 PLXBWHJQWKZRKG-UHFFFAOYSA-N 0.000 description 5
• 230000004075 alteration Effects 0.000 description 5
• 239000011230 binding agent Substances 0.000 description 5
• 210000004899 c-terminal region Anatomy 0.000 description 5
• 230000036952 cancer formation Effects 0.000 description 5
• 231100000504 carcinogenesis Toxicity 0.000 description 5
• 230000002860 competitive effect Effects 0.000 description 5
• 238000001415 gene therapy Methods 0.000 description 5
• 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 5
• 230000010189 intracellular transport Effects 0.000 description 5
• 239000002853 nucleic acid probe Substances 0.000 description 5
• 230000002018 overexpression Effects 0.000 description 5
• 239000010452 phosphate Substances 0.000 description 5
• 239000011780 sodium chloride Substances 0.000 description 5
• 239000000758 substrate Substances 0.000 description 5
• 239000006228 supernatant Substances 0.000 description 5
• 230000001225 therapeutic effect Effects 0.000 description 5
• 238000005406 washing Methods 0.000 description 5
• 102000002260 Alkaline Phosphatase Human genes 0.000 description 4
• 108020004774 Alkaline Phosphatase Proteins 0.000 description 4
• 102000006786 Chloride-Bicarbonate Antiporters Human genes 0.000 description 4
• 102000053602 DNA Human genes 0.000 description 4
• 230000004568 DNA-binding Effects 0.000 description 4
• KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 4
• WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
• VXKCPBPQEKKERH-IUCAKERBSA-N Gly-Arg-Pro Chemical compound NC(N)=NCCC[C@H](NC(=O)CN)C(=O)N1CCC[C@H]1C(O)=O VXKCPBPQEKKERH-IUCAKERBSA-N 0.000 description 4
• 101150107737 Hmga1 gene Proteins 0.000 description 4
• FUKDBQGFSJUXGX-RWMBFGLXSA-N Lys-Arg-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CCCCN)N)C(=O)O FUKDBQGFSJUXGX-RWMBFGLXSA-N 0.000 description 4
• 108020005187 Oligonucleotide Probes Proteins 0.000 description 4
• 108010029485 Protein Isoforms Proteins 0.000 description 4
• 102000001708 Protein Isoforms Human genes 0.000 description 4
• 108091006260 SLC4A2 Proteins 0.000 description 4
• 239000007983 Tris buffer Substances 0.000 description 4
• 206010046798 Uterine leiomyoma Diseases 0.000 description 4
• 238000009825 accumulation Methods 0.000 description 4
• 229960002685 biotin Drugs 0.000 description 4
• 235000020958 biotin Nutrition 0.000 description 4
• 239000011616 biotin Substances 0.000 description 4
• 230000022131 cell cycle Effects 0.000 description 4
• 210000003855 cell nucleus Anatomy 0.000 description 4
• 238000001516 cell proliferation assay Methods 0.000 description 4
• 239000000470 constituent Substances 0.000 description 4
• 208000035475 disorder Diseases 0.000 description 4
• 238000010494 dissociation reaction Methods 0.000 description 4
• 230000005593 dissociations Effects 0.000 description 4
• 231100000673 dose–response relationship Toxicity 0.000 description 4
• 238000010828 elution Methods 0.000 description 4
• 230000007717 exclusion Effects 0.000 description 4
• 239000008103 glucose Substances 0.000 description 4
• 230000012010 growth Effects 0.000 description 4
• 239000003102 growth factor Substances 0.000 description 4
• 238000002347 injection Methods 0.000 description 4
• 239000007924 injection Substances 0.000 description 4
• 210000003734 kidney Anatomy 0.000 description 4
• 201000010260 leiomyoma Diseases 0.000 description 4
• 239000003446 ligand Substances 0.000 description 4
• 210000004185 liver Anatomy 0.000 description 4
• 210000003712 lysosome Anatomy 0.000 description 4
• 230000001868 lysosomic effect Effects 0.000 description 4
• 229910001629 magnesium chloride Inorganic materials 0.000 description 4
• 230000007246 mechanism Effects 0.000 description 4
• 239000002609 medium Substances 0.000 description 4
• 230000009149 molecular binding Effects 0.000 description 4
• 239000002751 oligonucleotide probe Substances 0.000 description 4
• 239000002245 particle Substances 0.000 description 4
• NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 4
• 238000000746 purification Methods 0.000 description 4
• 230000002285 radioactive effect Effects 0.000 description 4
• 230000003252 repetitive effect Effects 0.000 description 4
• 238000003757 reverse transcription PCR Methods 0.000 description 4
• 238000000926 separation method Methods 0.000 description 4
• 238000003786 synthesis reaction Methods 0.000 description 4
• LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 4
• 201000007954 uterine fibroid Diseases 0.000 description 4
• USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 3
• 239000005695 Ammonium acetate Substances 0.000 description 3
• 102100027468 Anion exchange protein 2 Human genes 0.000 description 3
• 201000009030 Carcinoma Diseases 0.000 description 3
• 101000986346 Chironomus tentans High mobility group protein I Proteins 0.000 description 3
• 108010077544 Chromatin Proteins 0.000 description 3
• 229920001651 Cyanoacrylate Polymers 0.000 description 3
• OWOFCNWTMWOOJJ-WDSKDSINSA-N Gln-Glu Chemical compound NC(=O)CC[C@H](N)C(=O)N[C@@H](CCC(O)=O)C(O)=O OWOFCNWTMWOOJJ-WDSKDSINSA-N 0.000 description 3
• BUZMZDDKFCSKOT-CIUDSAMLSA-N Glu-Glu-Glu Chemical compound OC(=O)CC[C@H](N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(O)=O BUZMZDDKFCSKOT-CIUDSAMLSA-N 0.000 description 3
• KFMBRBPXHVMDFN-UWVGGRQHSA-N Gly-Arg-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)CN)CCCNC(N)=N KFMBRBPXHVMDFN-UWVGGRQHSA-N 0.000 description 3
• 102100022103 Histone-lysine N-methyltransferase 2A Human genes 0.000 description 3
• 101000736088 Homo sapiens PC4 and SFRS1-interacting protein Proteins 0.000 description 3
• 241000700588 Human alphaherpesvirus 1 Species 0.000 description 3
• 241000713772 Human immunodeficiency virus 1 Species 0.000 description 3
• JEYCTXHKTXCGPB-UHFFFAOYSA-N Methaqualone Chemical compound CC1=CC=CC=C1N1C(=O)C2=CC=CC=C2N=C1C JEYCTXHKTXCGPB-UHFFFAOYSA-N 0.000 description 3
• MWCLLHOVUTZFKS-UHFFFAOYSA-N Methyl cyanoacrylate Chemical compound COC(=O)C(=C)C#N MWCLLHOVUTZFKS-UHFFFAOYSA-N 0.000 description 3
• 108010077850 Nuclear Localization Signals Proteins 0.000 description 3
• 102000035195 Peptidases Human genes 0.000 description 3
• 108091005804 Peptidases Proteins 0.000 description 3
• 102000003992 Peroxidases Human genes 0.000 description 3
• AIOWVDNPESPXRB-YTWAJWBKSA-N Pro-Thr-Pro Chemical compound C[C@H]([C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)[C@@H]2CCCN2)O AIOWVDNPESPXRB-YTWAJWBKSA-N 0.000 description 3
• 239000004365 Protease Substances 0.000 description 3
• 102100032741 SET-binding protein Human genes 0.000 description 3
• 239000006180 TBST buffer Substances 0.000 description 3
• 241000700605 Viruses Species 0.000 description 3
• 230000002378 acidificating effect Effects 0.000 description 3
• KOSRFJWDECSPRO-UHFFFAOYSA-N alpha-L-glutamyl-L-glutamic acid Natural products OC(=O)CCC(N)C(=O)NC(CCC(O)=O)C(O)=O KOSRFJWDECSPRO-UHFFFAOYSA-N 0.000 description 3
• 229940043376 ammonium acetate Drugs 0.000 description 3
• 235000019257 ammonium acetate Nutrition 0.000 description 3
• 230000003321 amplification Effects 0.000 description 3
• 238000010171 animal model Methods 0.000 description 3
• 125000000129 anionic group Chemical group 0.000 description 3
• 230000001745 anti-biotin effect Effects 0.000 description 3
• 230000001093 anti-cancer Effects 0.000 description 3
• 230000008033 biological extinction Effects 0.000 description 3
• 239000000090 biomarker Substances 0.000 description 3
• 230000000903 blocking effect Effects 0.000 description 3
• 210000004204 blood vessel Anatomy 0.000 description 3
• 210000001124 body fluid Anatomy 0.000 description 3
• 239000010839 body fluid Substances 0.000 description 3
• 125000002091 cationic group Chemical group 0.000 description 3
• 238000004113 cell culture Methods 0.000 description 3
• 230000001413 cellular effect Effects 0.000 description 3
• 239000003153 chemical reaction reagent Substances 0.000 description 3
• HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol group Chemical group [C@@H]1(CC[C@H]2[C@@H]3CC=C4C[C@@H](O)CC[C@]4(C)[C@H]3CC[C@]12C)[C@H](C)CCCC(C)C HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 3
• 210000003483 chromatin Anatomy 0.000 description 3
• 230000002759 chromosomal effect Effects 0.000 description 3
• 210000000349 chromosome Anatomy 0.000 description 3
• 238000004132 cross linking Methods 0.000 description 3
• 238000000354 decomposition reaction Methods 0.000 description 3
• 238000012217 deletion Methods 0.000 description 3
• 229940079593 drug Drugs 0.000 description 3
• 230000013020 embryo development Effects 0.000 description 3
• 230000012202 endocytosis Effects 0.000 description 3
• 230000002255 enzymatic effect Effects 0.000 description 3
• 238000012869 ethanol precipitation Methods 0.000 description 3
• 239000007850 fluorescent dye Substances 0.000 description 3
• 210000000232 gallbladder Anatomy 0.000 description 3
• 108010055341 glutamyl-glutamic acid Proteins 0.000 description 3
• 102000043323 human PSIP1 Human genes 0.000 description 3
• 238000011813 knockout mouse model Methods 0.000 description 3
• 239000000463 material Substances 0.000 description 3
• 238000003199 nucleic acid amplification method Methods 0.000 description 3
• 210000004940 nucleus Anatomy 0.000 description 3
• 230000006320 pegylation Effects 0.000 description 3
• 239000008188 pellet Substances 0.000 description 3
• 108040007629 peroxidase activity proteins Proteins 0.000 description 3
• 150000003904 phospholipids Chemical class 0.000 description 3
• 239000013612 plasmid Substances 0.000 description 3
• 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 3
• 238000001556 precipitation Methods 0.000 description 3
• 238000004445 quantitative analysis Methods 0.000 description 3
• 230000000284 resting effect Effects 0.000 description 3
• 238000012552 review Methods 0.000 description 3
• 230000011664 signaling Effects 0.000 description 3
• 150000003384 small molecules Chemical class 0.000 description 3
• 230000006641 stabilisation Effects 0.000 description 3
• 238000011105 stabilization Methods 0.000 description 3
• 238000010254 subcutaneous injection Methods 0.000 description 3
• 239000000725 suspension Substances 0.000 description 3
• 230000009466 transformation Effects 0.000 description 3
• 208000029729 tumor suppressor gene on chromosome 11 Diseases 0.000 description 3
• 230000003612 virological effect Effects 0.000 description 3
• 239000011534 wash buffer Substances 0.000 description 3
• MSWZFWKMSRAUBD-RSVSWTKNSA-N (3r,4s,5s,6r)-3-amino-6-(hydroxymethyl)oxane-2,4,5-triol Chemical compound N[C@H]1C(O)O[C@H](CO)[C@@H](O)[C@H]1O MSWZFWKMSRAUBD-RSVSWTKNSA-N 0.000 description 2
• 102000040650 (ribonucleotides)n+m Human genes 0.000 description 2
• KSXTUUUQYQYKCR-LQDDAWAPSA-M 2,3-bis[[(z)-octadec-9-enoyl]oxy]propyl-trimethylazanium;chloride Chemical compound [Cl-].CCCCCCCC\C=C/CCCCCCCC(=O)OCC(C[N+](C)(C)C)OC(=O)CCCCCCC\C=C/CCCCCCCC KSXTUUUQYQYKCR-LQDDAWAPSA-M 0.000 description 2
• HBAQYPYDRFILMT-UHFFFAOYSA-N 8-[3-(1-cyclopropylpyrazol-4-yl)-1H-pyrazolo[4,3-d]pyrimidin-5-yl]-3-methyl-3,8-diazabicyclo[3.2.1]octan-2-one Chemical class C1(CC1)N1N=CC(=C1)C1=NNC2=C1N=C(N=C2)N1C2C(N(CC1CC2)C)=O HBAQYPYDRFILMT-UHFFFAOYSA-N 0.000 description 2
• RIIVUOJDDQXHRV-SRVKXCTJSA-N Arg-Lys-Gln Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(N)=O)C(O)=O RIIVUOJDDQXHRV-SRVKXCTJSA-N 0.000 description 2
• 108090001008 Avidin Proteins 0.000 description 2
• 241000894006 Bacteria Species 0.000 description 2
• 101100026251 Caenorhabditis elegans atf-2 gene Proteins 0.000 description 2
• 101100283604 Caenorhabditis elegans pigk-1 gene Proteins 0.000 description 2
• 102000014914 Carrier Proteins Human genes 0.000 description 2
• 108010078791 Carrier Proteins Proteins 0.000 description 2
• 108091026890 Coding region Proteins 0.000 description 2
• 108020004705 Codon Proteins 0.000 description 2
• 239000003298 DNA probe Substances 0.000 description 2
• 230000033616 DNA repair Effects 0.000 description 2
• XQDGOJPVMSWZSO-SRVKXCTJSA-N Gln-Pro-Leu Chemical compound CC(C)C[C@@H](C(=O)O)NC(=O)[C@@H]1CCCN1C(=O)[C@H](CCC(=O)N)N XQDGOJPVMSWZSO-SRVKXCTJSA-N 0.000 description 2
• 108700039144 HMGA1b Proteins 0.000 description 2
• 102000000849 HMGB Proteins Human genes 0.000 description 2
• 108010001860 HMGB Proteins Proteins 0.000 description 2
• 108010044429 HMGN Proteins Proteins 0.000 description 2
• 102000006491 HMGN Proteins Human genes 0.000 description 2
• 101100395337 Homo sapiens HMGA2 gene Proteins 0.000 description 2
• 101000818569 Homo sapiens Zinc finger and BTB domain-containing protein 24 Proteins 0.000 description 2
• 101000785605 Homo sapiens Zinc finger protein 653 Proteins 0.000 description 2
• 241000701044 Human gammaherpesvirus 4 Species 0.000 description 2
• VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
• ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 2
• KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 2
• ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 2
• AAORVPFVUIHEAB-YUMQZZPRSA-N Lys-Asp-Gly Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CC(O)=O)C(=O)NCC(O)=O AAORVPFVUIHEAB-YUMQZZPRSA-N 0.000 description 2
• OAPNERBWQWUPTI-YUMQZZPRSA-N Lys-Gln Chemical compound NCCCC[C@H](N)C(=O)N[C@H](C(O)=O)CCC(N)=O OAPNERBWQWUPTI-YUMQZZPRSA-N 0.000 description 2
• LUTDBHBIHHREDC-IHRRRGAJSA-N Lys-Pro-Lys Chemical compound NCCCC[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCCN)C(O)=O LUTDBHBIHHREDC-IHRRRGAJSA-N 0.000 description 2
• TVHCDSBMFQYPNA-RHYQMDGZSA-N Lys-Thr-Arg Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O TVHCDSBMFQYPNA-RHYQMDGZSA-N 0.000 description 2
• 102000003945 NF-kappa B Human genes 0.000 description 2
• 108010057466 NF-kappa B Proteins 0.000 description 2
• 108700019961 Neoplasm Genes Proteins 0.000 description 2
• 102000048850 Neoplasm Genes Human genes 0.000 description 2
• 208000009869 Neu-Laxova syndrome Diseases 0.000 description 2
• 208000008589 Obesity Diseases 0.000 description 2
• 108091000080 Phosphotransferase Proteins 0.000 description 2
• 102100026869 RNA-binding protein MEX3B Human genes 0.000 description 2
• 239000012980 RPMI-1640 medium Substances 0.000 description 2
• 241000700159 Rattus Species 0.000 description 2
• 101710194492 SET-binding protein Proteins 0.000 description 2
• 206010039491 Sarcoma Diseases 0.000 description 2
• 108020004459 Small interfering RNA Proteins 0.000 description 2
• HJOSVGCWOTYJFG-WDCWCFNPSA-N Thr-Glu-Lys Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCCCN)C(=O)O)N)O HJOSVGCWOTYJFG-WDCWCFNPSA-N 0.000 description 2
• AQAMPXBRJJWPNI-JHEQGTHGSA-N Thr-Gly-Glu Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N[C@@H](CCC(O)=O)C(O)=O AQAMPXBRJJWPNI-JHEQGTHGSA-N 0.000 description 2
• 102100023132 Transcription factor Jun Human genes 0.000 description 2
• ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 2
• 108700005077 Viral Genes Proteins 0.000 description 2
• QWXOJIDBSHLIFI-UHFFFAOYSA-N [3-(1-chloro-3'-methoxyspiro[adamantane-4,4'-dioxetane]-3'-yl)phenyl] dihydrogen phosphate Chemical compound O1OC2(C3CC4CC2CC(Cl)(C4)C3)C1(OC)C1=CC=CC(OP(O)(O)=O)=C1 QWXOJIDBSHLIFI-UHFFFAOYSA-N 0.000 description 2
• 150000007513 acids Chemical class 0.000 description 2
• 230000004913 activation Effects 0.000 description 2
• OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 2
• 239000011543 agarose gel Substances 0.000 description 2
• 210000004102 animal cell Anatomy 0.000 description 2
• 238000000137 annealing Methods 0.000 description 2
• 230000001640 apoptogenic effect Effects 0.000 description 2
• 230000006907 apoptotic process Effects 0.000 description 2
• 108010062796 arginyllysine Proteins 0.000 description 2
• 239000012131 assay buffer Substances 0.000 description 2
• 230000008901 benefit Effects 0.000 description 2
• 210000004369 blood Anatomy 0.000 description 2
• 239000008280 blood Substances 0.000 description 2
• 230000037396 body weight Effects 0.000 description 2
• 208000035269 cancer or benign tumor Diseases 0.000 description 2
• 230000030833 cell death Effects 0.000 description 2
• 230000032823 cell division Effects 0.000 description 2
• 230000004656 cell transport Effects 0.000 description 2
• 230000019522 cellular metabolic process Effects 0.000 description 2
• 230000007541 cellular toxicity Effects 0.000 description 2
• 238000003776 cleavage reaction Methods 0.000 description 2
• 238000009833 condensation Methods 0.000 description 2
• 230000005494 condensation Effects 0.000 description 2
• 230000001276 controlling effect Effects 0.000 description 2
• 230000009260 cross reactivity Effects 0.000 description 2
• 230000001086 cytosolic effect Effects 0.000 description 2
• 230000037430 deletion Effects 0.000 description 2
• 230000001419 dependent effect Effects 0.000 description 2
• 230000004069 differentiation Effects 0.000 description 2
• 230000028023 exocytosis Effects 0.000 description 2
• 239000012530 fluid Substances 0.000 description 2
• 238000001502 gel electrophoresis Methods 0.000 description 2
• 230000002068 genetic effect Effects 0.000 description 2
• 108010089804 glycyl-threonine Proteins 0.000 description 2
• 210000002288 golgi apparatus Anatomy 0.000 description 2
• 125000000487 histidyl group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C([H])=N1 0.000 description 2
• 230000002209 hydrophobic effect Effects 0.000 description 2
• 238000010348 incorporation Methods 0.000 description 2
• 230000004941 influx Effects 0.000 description 2
• 239000003112 inhibitor Substances 0.000 description 2
• 238000003780 insertion Methods 0.000 description 2
• 230000037431 insertion Effects 0.000 description 2
• 150000002500 ions Chemical class 0.000 description 2
• 108010064235 lysylglycine Proteins 0.000 description 2
• 210000002540 macrophage Anatomy 0.000 description 2
• 210000004962 mammalian cell Anatomy 0.000 description 2
• 239000003550 marker Substances 0.000 description 2
• 238000005259 measurement Methods 0.000 description 2
• 230000010534 mechanism of action Effects 0.000 description 2
• 239000013642 negative control Substances 0.000 description 2
• 210000005170 neoplastic cell Anatomy 0.000 description 2
• 230000001613 neoplastic effect Effects 0.000 description 2
• 238000013421 nuclear magnetic resonance imaging Methods 0.000 description 2
• 235000020824 obesity Nutrition 0.000 description 2
• 230000003204 osmotic effect Effects 0.000 description 2
• 230000008506 pathogenesis Effects 0.000 description 2
• 239000002953 phosphate buffered saline Substances 0.000 description 2
• 102000020233 phosphotransferase Human genes 0.000 description 2
• 238000000053 physical method Methods 0.000 description 2
• 229920005643 polyisobutyl cyanoacrylate Polymers 0.000 description 2
• 229920000642 polymer Polymers 0.000 description 2
• 229920000136 polysorbate Polymers 0.000 description 2
• 238000010837 poor prognosis Methods 0.000 description 2
• 238000002360 preparation method Methods 0.000 description 2
• 238000004393 prognosis Methods 0.000 description 2
• 108010029020 prolylglycine Proteins 0.000 description 2
• 238000011002 quantification Methods 0.000 description 2
• 210000000664 rectum Anatomy 0.000 description 2
• 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 2
• 230000001105 regulatory effect Effects 0.000 description 2
• 238000011160 research Methods 0.000 description 2
• 239000012723 sample buffer Substances 0.000 description 2
• 230000007017 scission Effects 0.000 description 2
• 239000002904 solvent Substances 0.000 description 2
• 230000009870 specific binding Effects 0.000 description 2
• 239000007929 subcutaneous injection Substances 0.000 description 2
• 230000008685 targeting Effects 0.000 description 2
• 231100000331 toxic Toxicity 0.000 description 2
• 230000002588 toxic effect Effects 0.000 description 2
• 230000007723 transport mechanism Effects 0.000 description 2
• 210000004509 vascular smooth muscle cell Anatomy 0.000 description 2
• XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
• 238000001262 western blot Methods 0.000 description 2
• 239000002023 wood Substances 0.000 description 2
• SSCSSDNTQJGTJT-UHFFFAOYSA-N (3,6-dihydroxy-1-methyl-2,3-dihydroindol-5-yl)iminourea Chemical compound CN1CC(O)C2=CC(N=NC(N)=O)=C(O)C=C12 SSCSSDNTQJGTJT-UHFFFAOYSA-N 0.000 description 1
• LDGWQMRUWMSZIU-LQDDAWAPSA-M 2,3-bis[(z)-octadec-9-enoxy]propyl-trimethylazanium;chloride Chemical compound [Cl-].CCCCCCCC\C=C/CCCCCCCCOCC(C[N+](C)(C)C)OCCCCCCCC\C=C/CCCCCCCC LDGWQMRUWMSZIU-LQDDAWAPSA-M 0.000 description 1
• 101710112984 20 kDa protein Proteins 0.000 description 1
• FWBHETKCLVMNFS-UHFFFAOYSA-N 4',6-Diamino-2-phenylindol Chemical compound C1=CC(C(=N)N)=CC=C1C1=CC2=CC=C(C(N)=N)C=C2N1 FWBHETKCLVMNFS-UHFFFAOYSA-N 0.000 description 1
• ITZMJCSORYKOSI-AJNGGQMLSA-N APGPR Enterostatin Chemical compound C[C@H](N)C(=O)N1CCC[C@H]1C(=O)NCC(=O)N1[C@H](C(=O)N[C@@H](CCCN=C(N)N)C(O)=O)CCC1 ITZMJCSORYKOSI-AJNGGQMLSA-N 0.000 description 1
• 208000006678 Abdominal Neoplasms Diseases 0.000 description 1
• 241001514645 Agonis Species 0.000 description 1
• 101710145634 Antigen 1 Proteins 0.000 description 1
• 108020005544 Antisense RNA Proteins 0.000 description 1
• AUFHLLPVPSMEOG-YUMQZZPRSA-N Arg-Gly-Glu Chemical compound NC(N)=NCCC[C@H](N)C(=O)NCC(=O)N[C@@H](CCC(O)=O)C(O)=O AUFHLLPVPSMEOG-YUMQZZPRSA-N 0.000 description 1
• BTJVOUQWFXABOI-IHRRRGAJSA-N Arg-Lys-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](N)CCCNC(N)=N BTJVOUQWFXABOI-IHRRRGAJSA-N 0.000 description 1
• 239000004475 Arginine Substances 0.000 description 1
• NYGILGUOUOXGMJ-YUMQZZPRSA-N Asn-Lys-Gly Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCCN)C(=O)NCC(O)=O NYGILGUOUOXGMJ-YUMQZZPRSA-N 0.000 description 1
• AWXDRZJQCVHCIT-DCAQKATOSA-N Asn-Pro-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](N)CC(N)=O AWXDRZJQCVHCIT-DCAQKATOSA-N 0.000 description 1
• 208000037260 Atherosclerotic Plaque Diseases 0.000 description 1
• UTFVTJYLPLJXBI-UHFFFAOYSA-N Austin Natural products CC1OC(=O)C2C3C(C)(OC(=O)C13O)C(OC(=O)C)C4=C(C)C5(CCC24C)C=CC(=O)OC5(C)C UTFVTJYLPLJXBI-UHFFFAOYSA-N 0.000 description 1
• 102000004506 Blood Proteins Human genes 0.000 description 1
• 108010017384 Blood Proteins Proteins 0.000 description 1
• 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
• 239000002126 C01EB10 - Adenosine Substances 0.000 description 1
• 102100024156 Cadherin-12 Human genes 0.000 description 1
• 101710196882 Cadherin-12 Proteins 0.000 description 1
• OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
• UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
• 108090000994 Catalytic RNA Proteins 0.000 description 1
• 102000053642 Catalytic RNA Human genes 0.000 description 1
• ZEOWTGPWHLSLOG-UHFFFAOYSA-N Cc1ccc(cc1-c1ccc2c(n[nH]c2c1)-c1cnn(c1)C1CC1)C(=O)Nc1cccc(c1)C(F)(F)F Chemical compound Cc1ccc(cc1-c1ccc2c(n[nH]c2c1)-c1cnn(c1)C1CC1)C(=O)Nc1cccc(c1)C(F)(F)F ZEOWTGPWHLSLOG-UHFFFAOYSA-N 0.000 description 1
• 108010059892 Cellulase Proteins 0.000 description 1
• 108091006146 Channels Proteins 0.000 description 1
• VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
• 102100032920 Chromobox protein homolog 2 Human genes 0.000 description 1
• 102000008186 Collagen Human genes 0.000 description 1
• 108010035532 Collagen Proteins 0.000 description 1
• 102000004127 Cytokines Human genes 0.000 description 1
• 108090000695 Cytokines Proteins 0.000 description 1
• 150000008574 D-amino acids Chemical class 0.000 description 1
• 230000005778 DNA damage Effects 0.000 description 1
• 231100000277 DNA damage Toxicity 0.000 description 1
• 108010053770 Deoxyribonucleases Proteins 0.000 description 1
• 102000016911 Deoxyribonucleases Human genes 0.000 description 1
• 206010061818 Disease progression Diseases 0.000 description 1
• 238000002965 ELISA Methods 0.000 description 1
• 208000005189 Embolism Diseases 0.000 description 1
• 241000196324 Embryophyta Species 0.000 description 1
• 101000658547 Escherichia coli (strain K12) Type I restriction enzyme EcoKI endonuclease subunit Proteins 0.000 description 1
• 101000658543 Escherichia coli Type I restriction enzyme EcoAI endonuclease subunit Proteins 0.000 description 1
• 101000658546 Escherichia coli Type I restriction enzyme EcoEI endonuclease subunit Proteins 0.000 description 1
• 101000658530 Escherichia coli Type I restriction enzyme EcoR124II endonuclease subunit Proteins 0.000 description 1
• 101000658540 Escherichia coli Type I restriction enzyme EcoprrI endonuclease subunit Proteins 0.000 description 1
• 241000206602 Eukaryota Species 0.000 description 1
• 208000007659 Fibroadenoma Diseases 0.000 description 1
• 102000018233 Fibroblast Growth Factor Human genes 0.000 description 1
• 108050007372 Fibroblast Growth Factor Proteins 0.000 description 1
• 102100023593 Fibroblast growth factor receptor 1 Human genes 0.000 description 1
• 101710182386 Fibroblast growth factor receptor 1 Proteins 0.000 description 1
• 102100023600 Fibroblast growth factor receptor 2 Human genes 0.000 description 1
• 101710182389 Fibroblast growth factor receptor 2 Proteins 0.000 description 1
• 102100039818 Frizzled-5 Human genes 0.000 description 1
• 101710140951 Frizzled-5 Proteins 0.000 description 1
• 102000013446 GTP Phosphohydrolases Human genes 0.000 description 1
• 108091006109 GTPases Proteins 0.000 description 1
• 108010010803 Gelatin Proteins 0.000 description 1
• SNLOOPZHAQDMJG-CIUDSAMLSA-N Gln-Glu-Glu Chemical compound NC(=O)CC[C@H](N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(O)=O SNLOOPZHAQDMJG-CIUDSAMLSA-N 0.000 description 1
• VOLVNCMGXWDDQY-LPEHRKFASA-N Gln-Glu-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CCC(=O)N)N)C(=O)O VOLVNCMGXWDDQY-LPEHRKFASA-N 0.000 description 1
• JEFZIKRIDLHOIF-BYPYZUCNSA-N Gln-Gly Chemical compound NC(=O)CC[C@H](N)C(=O)NCC(O)=O JEFZIKRIDLHOIF-BYPYZUCNSA-N 0.000 description 1
• HPCOBEHVEHWREJ-DCAQKATOSA-N Gln-Lys-Glu Chemical compound [H]N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(O)=O)C(O)=O HPCOBEHVEHWREJ-DCAQKATOSA-N 0.000 description 1
• JRHPEMVLTRADLJ-AVGNSLFASA-N Gln-Lys-Lys Chemical compound C(CCN)C[C@@H](C(=O)N[C@@H](CCCCN)C(=O)O)NC(=O)[C@H](CCC(=O)N)N JRHPEMVLTRADLJ-AVGNSLFASA-N 0.000 description 1
• NJMYZEJORPYOTO-BQBZGAKWSA-N Gln-Pro Chemical compound NC(=O)CC[C@H](N)C(=O)N1CCC[C@H]1C(O)=O NJMYZEJORPYOTO-BQBZGAKWSA-N 0.000 description 1
• MFORDNZDKAVNSR-SRVKXCTJSA-N Gln-Pro-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](N)CCC(N)=O MFORDNZDKAVNSR-SRVKXCTJSA-N 0.000 description 1
• KOSRFJWDECSPRO-WDSKDSINSA-N Glu-Glu Chemical compound OC(=O)CC[C@H](N)C(=O)N[C@@H](CCC(O)=O)C(O)=O KOSRFJWDECSPRO-WDSKDSINSA-N 0.000 description 1
• PHONAZGUEGIOEM-GLLZPBPUSA-N Glu-Glu-Thr Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O PHONAZGUEGIOEM-GLLZPBPUSA-N 0.000 description 1
• YBTCBQBIJKGSJP-BQBZGAKWSA-N Glu-Pro Chemical compound OC(=O)CC[C@H](N)C(=O)N1CCC[C@H]1C(O)=O YBTCBQBIJKGSJP-BQBZGAKWSA-N 0.000 description 1
• PABFFPWEJMEVEC-JGVFFNPUSA-N Gly-Gln-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CCC(=O)N)NC(=O)CN)C(=O)O PABFFPWEJMEVEC-JGVFFNPUSA-N 0.000 description 1
• POJJAZJHBGXEGM-YUMQZZPRSA-N Gly-Ser-Lys Chemical compound C(CCN)C[C@@H](C(=O)O)NC(=O)[C@H](CO)NC(=O)CN POJJAZJHBGXEGM-YUMQZZPRSA-N 0.000 description 1
• JZNWSCPGTDBMEW-UHFFFAOYSA-N Glycerophosphorylethanolamin Natural products NCCOP(O)(=O)OCC(O)CO JZNWSCPGTDBMEW-UHFFFAOYSA-N 0.000 description 1
• 229920002527 Glycogen Polymers 0.000 description 1
• 102000049982 HMGA2 Human genes 0.000 description 1
• 108700010012 HMGN1 Proteins 0.000 description 1
• 101000658545 Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) Type I restriction enyme HindI endonuclease subunit Proteins 0.000 description 1
• 208000002927 Hamartoma Diseases 0.000 description 1
• 102100037907 High mobility group protein B1 Human genes 0.000 description 1
• 101710168537 High mobility group protein B1 Proteins 0.000 description 1
• 108050002855 Histone-lysine N-methyltransferase 2A Proteins 0.000 description 1
• 102100039489 Histone-lysine N-methyltransferase, H3 lysine-79 specific Human genes 0.000 description 1
• 108010033040 Histones Proteins 0.000 description 1
• 101000797586 Homo sapiens Chromobox protein homolog 2 Proteins 0.000 description 1
• 101000980066 Homo sapiens Cilia- and flagella-associated protein 410 Proteins 0.000 description 1
• 101001056455 Homo sapiens Endosome-associated-trafficking regulator 1 Proteins 0.000 description 1
• 101000986379 Homo sapiens High mobility group protein HMGI-C Proteins 0.000 description 1
• 101000963360 Homo sapiens Histone-lysine N-methyltransferase, H3 lysine-79 specific Proteins 0.000 description 1
• 101100456961 Homo sapiens MEX3B gene Proteins 0.000 description 1
• 101001047090 Homo sapiens Potassium voltage-gated channel subfamily H member 2 Proteins 0.000 description 1
• 101000611663 Homo sapiens Prolargin Proteins 0.000 description 1
• 101000629813 Homo sapiens RNA-binding protein MEX3B Proteins 0.000 description 1
• 101000703425 Homo sapiens Rho GTPase-activating protein 39 Proteins 0.000 description 1
• 101000703439 Homo sapiens Rho GTPase-activating protein 42 Proteins 0.000 description 1
• 101001050288 Homo sapiens Transcription factor Jun Proteins 0.000 description 1
• 101000801254 Homo sapiens Tumor necrosis factor receptor superfamily member 16 Proteins 0.000 description 1
• 101000801232 Homo sapiens Tumor necrosis factor receptor superfamily member 1B Proteins 0.000 description 1
• 102000003839 Human Proteins Human genes 0.000 description 1
• 108090000144 Human Proteins Proteins 0.000 description 1
• 101150027427 ICP4 gene Proteins 0.000 description 1
• 102000018071 Immunoglobulin Fc Fragments Human genes 0.000 description 1
• 108010091135 Immunoglobulin Fc Fragments Proteins 0.000 description 1
• 206010061216 Infarction Diseases 0.000 description 1
• 206010061218 Inflammation Diseases 0.000 description 1
• 108010076876 Keratins Proteins 0.000 description 1
• 102000011782 Keratins Human genes 0.000 description 1
• 102100022251 Kinesin-like protein KIFC2 Human genes 0.000 description 1
• 101710134602 Kinesin-like protein KIFC2 Proteins 0.000 description 1
• 150000008575 L-amino acids Chemical class 0.000 description 1
• 108090000364 Ligases Proteins 0.000 description 1
• 102000003960 Ligases Human genes 0.000 description 1
• 108090001030 Lipoproteins Proteins 0.000 description 1
• 102000004895 Lipoproteins Human genes 0.000 description 1
• 208000028018 Lymphocytic leukaemia Diseases 0.000 description 1
• ZQCVMVCVPFYXHZ-SRVKXCTJSA-N Lys-Asn-Lys Chemical compound NCCCC[C@H](N)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@H](C(O)=O)CCCCN ZQCVMVCVPFYXHZ-SRVKXCTJSA-N 0.000 description 1
• MRWXLRGAFDOILG-DCAQKATOSA-N Lys-Gln-Gln Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(N)=O)C(O)=O MRWXLRGAFDOILG-DCAQKATOSA-N 0.000 description 1
• FHIAJWBDZVHLAH-YUMQZZPRSA-N Lys-Gly-Ser Chemical compound NCCCC[C@H](N)C(=O)NCC(=O)N[C@@H](CO)C(O)=O FHIAJWBDZVHLAH-YUMQZZPRSA-N 0.000 description 1
• NVGBPTNZLWRQSY-UWVGGRQHSA-N Lys-Lys Chemical compound NCCCC[C@H](N)C(=O)N[C@H](C(O)=O)CCCCN NVGBPTNZLWRQSY-UWVGGRQHSA-N 0.000 description 1
• YXPJCVNIDDKGOE-MELADBBJSA-N Lys-Lys-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CCCCN)NC(=O)[C@H](CCCCN)N)C(=O)O YXPJCVNIDDKGOE-MELADBBJSA-N 0.000 description 1
• CAVRAQIDHUPECU-UVOCVTCTSA-N Lys-Thr-Thr Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O CAVRAQIDHUPECU-UVOCVTCTSA-N 0.000 description 1
• AWMMBHDKERMOID-YTQUADARSA-N Lys-Trp-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CC2=CNC3=CC=CC=C32)NC(=O)[C@H](CCCCN)N)C(=O)O AWMMBHDKERMOID-YTQUADARSA-N 0.000 description 1
• FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
• 206010064912 Malignant transformation Diseases 0.000 description 1
• 241001446467 Mama Species 0.000 description 1
• 102100030200 Matrix metalloproteinase-16 Human genes 0.000 description 1
• 108090000561 Matrix metalloproteinase-16 Proteins 0.000 description 1
• 102000012750 Membrane Glycoproteins Human genes 0.000 description 1
• 108010090054 Membrane Glycoproteins Proteins 0.000 description 1
• 102000003939 Membrane transport proteins Human genes 0.000 description 1
• 108090000301 Membrane transport proteins Proteins 0.000 description 1
• 101000658548 Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) Putative type I restriction enzyme MjaIXP endonuclease subunit Proteins 0.000 description 1
• 101000658542 Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) Putative type I restriction enzyme MjaVIIIP endonuclease subunit Proteins 0.000 description 1
• 101000658529 Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) Putative type I restriction enzyme MjaVIIP endonuclease subunit Proteins 0.000 description 1
• 101710159910 Movement protein Proteins 0.000 description 1
• 241001529936 Murinae Species 0.000 description 1
• 241000699660 Mus musculus Species 0.000 description 1
• 102100038895 Myc proto-oncogene protein Human genes 0.000 description 1
• 101710135898 Myc proto-oncogene protein Proteins 0.000 description 1
• 239000000020 Nitrocellulose Substances 0.000 description 1
• 102000007999 Nuclear Proteins Human genes 0.000 description 1
• 108010089610 Nuclear Proteins Proteins 0.000 description 1
• 102000011931 Nucleoproteins Human genes 0.000 description 1
• 108010061100 Nucleoproteins Proteins 0.000 description 1
• 102000043276 Oncogene Human genes 0.000 description 1
• 102100036220 PC4 and SFRS1-interacting protein Human genes 0.000 description 1
• 229930040373 Paraformaldehyde Natural products 0.000 description 1
• 108010089430 Phosphoproteins Proteins 0.000 description 1
• 102000007982 Phosphoproteins Human genes 0.000 description 1
• 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 1
• 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 1
• ZYFVNVRFVHJEIU-UHFFFAOYSA-N PicoGreen Chemical compound CN(C)CCCN(CCCN(C)C)C1=CC(=CC2=[N+](C3=CC=CC=C3S2)C)C2=CC=CC=C2N1C1=CC=CC=C1 ZYFVNVRFVHJEIU-UHFFFAOYSA-N 0.000 description 1
• 239000004952 Polyamide Substances 0.000 description 1
• 239000004698 Polyethylene Substances 0.000 description 1
• 108010021757 Polynucleotide 5'-Hydroxyl-Kinase Proteins 0.000 description 1
• 102000008422 Polynucleotide 5'-hydroxyl-kinase Human genes 0.000 description 1
• 102000004257 Potassium Channel Human genes 0.000 description 1
• 102100022807 Potassium voltage-gated channel subfamily H member 2 Human genes 0.000 description 1
• BNBBNGZZKQUWCD-IUCAKERBSA-N Pro-Arg-Gly Chemical compound NC(N)=NCCC[C@@H](C(=O)NCC(O)=O)NC(=O)[C@@H]1CCCN1 BNBBNGZZKQUWCD-IUCAKERBSA-N 0.000 description 1
• AFXCXDQNRXTSBD-FJXKBIBVSA-N Pro-Gly-Thr Chemical compound [H]N1CCC[C@H]1C(=O)NCC(=O)N[C@@H]([C@@H](C)O)C(O)=O AFXCXDQNRXTSBD-FJXKBIBVSA-N 0.000 description 1
• RVQDZELMXZRSSI-IUCAKERBSA-N Pro-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@@H]1CCCN1 RVQDZELMXZRSSI-IUCAKERBSA-N 0.000 description 1
• JUJCUYWRJMFJJF-AVGNSLFASA-N Pro-Lys-Arg Chemical compound NC(N)=NCCC[C@@H](C(O)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@@H]1CCCN1 JUJCUYWRJMFJJF-AVGNSLFASA-N 0.000 description 1
• XQPHBAKJJJZOBX-SRVKXCTJSA-N Pro-Lys-Glu Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(O)=O)C(O)=O XQPHBAKJJJZOBX-SRVKXCTJSA-N 0.000 description 1
• ABSSTGUCBCDKMU-UWVGGRQHSA-N Pro-Lys-Gly Chemical compound NCCCC[C@@H](C(=O)NCC(O)=O)NC(=O)[C@@H]1CCCN1 ABSSTGUCBCDKMU-UWVGGRQHSA-N 0.000 description 1
• 102100027584 Protein c-Fos Human genes 0.000 description 1
• 108010071563 Proto-Oncogene Proteins c-fos Proteins 0.000 description 1
• 108091008103 RNA aptamers Proteins 0.000 description 1
• 108091030071 RNAI Proteins 0.000 description 1
• 101100480477 Rattus norvegicus Taar7d gene Proteins 0.000 description 1
• 230000010799 Receptor Interactions Effects 0.000 description 1
• 102100030748 Rho GTPase-activating protein 39 Human genes 0.000 description 1
• 102100030757 Rho GTPase-activating protein 42 Human genes 0.000 description 1
• 108091078243 Rho family Proteins 0.000 description 1
• 102000042463 Rho family Human genes 0.000 description 1
• 108091028664 Ribonucleotide Proteins 0.000 description 1
• UOLGINIHBRIECN-FXQIFTODSA-N Ser-Glu-Glu Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(O)=O UOLGINIHBRIECN-FXQIFTODSA-N 0.000 description 1
• MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
• 108010034546 Serratia marcescens nuclease Proteins 0.000 description 1
• 241000700584 Simplexvirus Species 0.000 description 1
• 102100030259 Spermatogenesis-associated protein 4 Human genes 0.000 description 1
• 101710147961 Spermatogenesis-associated protein 4 Proteins 0.000 description 1
• 101001042773 Staphylococcus aureus (strain COL) Type I restriction enzyme SauCOLORF180P endonuclease subunit Proteins 0.000 description 1
• 101000838760 Staphylococcus aureus (strain MRSA252) Type I restriction enzyme SauMRSORF196P endonuclease subunit Proteins 0.000 description 1
• 101000838761 Staphylococcus aureus (strain MSSA476) Type I restriction enzyme SauMSSORF170P endonuclease subunit Proteins 0.000 description 1
• 101000838758 Staphylococcus aureus (strain MW2) Type I restriction enzyme SauMW2ORF169P endonuclease subunit Proteins 0.000 description 1
• 101001042566 Staphylococcus aureus (strain Mu50 / ATCC 700699) Type I restriction enzyme SauMu50ORF195P endonuclease subunit Proteins 0.000 description 1
• 101000838763 Staphylococcus aureus (strain N315) Type I restriction enzyme SauN315I endonuclease subunit Proteins 0.000 description 1
• 101000838759 Staphylococcus epidermidis (strain ATCC 35984 / RP62A) Type I restriction enzyme SepRPIP endonuclease subunit Proteins 0.000 description 1
• 101000838756 Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229 / NCIMB 8711 / NCTC 7292 / S-41) Type I restriction enzyme SsaAORF53P endonuclease subunit Proteins 0.000 description 1
• 229920002472 Starch Polymers 0.000 description 1
• 238000000692 Student's t-test Methods 0.000 description 1
• 210000001744 T-lymphocyte Anatomy 0.000 description 1
• MXDOAJQRJBMGMO-FJXKBIBVSA-N Thr-Pro-Gly Chemical compound C[C@@H](O)[C@H](N)C(=O)N1CCC[C@H]1C(=O)NCC(O)=O MXDOAJQRJBMGMO-FJXKBIBVSA-N 0.000 description 1
• ZESGVALRVJIVLZ-VFCFLDTKSA-N Thr-Thr-Pro Chemical compound C[C@H]([C@@H](C(=O)N[C@@H]([C@@H](C)O)C(=O)N1CCC[C@@H]1C(=O)O)N)O ZESGVALRVJIVLZ-VFCFLDTKSA-N 0.000 description 1
• 108091036066 Three prime untranslated region Proteins 0.000 description 1
• 108010018242 Transcription Factor AP-1 Proteins 0.000 description 1
• 101710150448 Transcriptional regulator Myc Proteins 0.000 description 1
• 239000007997 Tricine buffer Substances 0.000 description 1
• 102000004243 Tubulin Human genes 0.000 description 1
• 108090000704 Tubulin Proteins 0.000 description 1
• 102000044209 Tumor Suppressor Genes Human genes 0.000 description 1
• 108700025716 Tumor Suppressor Genes Proteins 0.000 description 1
• 101710187830 Tumor necrosis factor receptor superfamily member 1B Proteins 0.000 description 1
• 241000145297 Vanni Species 0.000 description 1
• 108020005202 Viral DNA Proteins 0.000 description 1
• 101710090398 Viral interleukin-10 homolog Proteins 0.000 description 1
• 108010046377 Whey Proteins Proteins 0.000 description 1
• HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
• 101710096165 Zinc finger and BTB domain-containing protein 24 Proteins 0.000 description 1
• 102100021129 Zinc finger and BTB domain-containing protein 24 Human genes 0.000 description 1
• 101710185494 Zinc finger protein Proteins 0.000 description 1
• 102100023597 Zinc finger protein 816 Human genes 0.000 description 1
• DEMDOYQPCDXCEB-WLEVADLXSA-N [(1S,2R,5S,8R,9R,12S,13S)-12-hydroxy-2,2',2',6,9,13-hexamethyl-16-methylidene-6',11,15-trioxospiro[10,14-dioxatetracyclo[7.6.1.01,12.02,7]hexadec-6-ene-5,3'-pyran]-8-yl] acetate Chemical compound C1([C@@H](OC(C)=O)[C@@]2(C)C(=C)[C@]3([C@@]1(CC1)C)C(=O)O[C@H]([C@@]3(C(=O)O2)O)C)=C(C)[C@]21C=CC(=O)OC2(C)C DEMDOYQPCDXCEB-WLEVADLXSA-N 0.000 description 1
• 230000002159 abnormal effect Effects 0.000 description 1
• 239000012190 activator Substances 0.000 description 1
• 230000006978 adaptation Effects 0.000 description 1
• 229960005305 adenosine Drugs 0.000 description 1
• 210000001789 adipocyte Anatomy 0.000 description 1
• 210000000577 adipose tissue Anatomy 0.000 description 1
• 239000000443 aerosol Substances 0.000 description 1
• 230000002776 aggregation Effects 0.000 description 1
• 238000004220 aggregation Methods 0.000 description 1
• 125000003277 amino group Chemical group 0.000 description 1
• MDFFNEOEWAXZRQ-UHFFFAOYSA-N aminyl Chemical compound [NH2] MDFFNEOEWAXZRQ-UHFFFAOYSA-N 0.000 description 1
• 230000033115 angiogenesis Effects 0.000 description 1
• 239000002870 angiogenesis inducing agent Substances 0.000 description 1
• 239000004037 angiogenesis inhibitor Substances 0.000 description 1
• 229940121369 angiogenesis inhibitor Drugs 0.000 description 1
• 208000028435 angiomyxoma Diseases 0.000 description 1
• 150000001450 anions Chemical class 0.000 description 1
• 230000000840 anti-viral effect Effects 0.000 description 1
• 239000000074 antisense oligonucleotide Substances 0.000 description 1
• 238000012230 antisense oligonucleotides Methods 0.000 description 1
• 239000007864 aqueous solution Substances 0.000 description 1
• 108010009111 arginyl-glycyl-glutamic acid Proteins 0.000 description 1
• 108010060035 arginylproline Proteins 0.000 description 1
• 238000003491 array Methods 0.000 description 1
• 230000003416 augmentation Effects 0.000 description 1
• 230000001580 bacterial effect Effects 0.000 description 1
• WQZGKKKJIJFFOK-FPRJBGLDSA-N beta-D-galactose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-FPRJBGLDSA-N 0.000 description 1
• 229920002988 biodegradable polymer Polymers 0.000 description 1
• 239000004621 biodegradable polymer Substances 0.000 description 1
• 239000012472 biological sample Substances 0.000 description 1
• 238000001574 biopsy Methods 0.000 description 1
• 210000004556 brain Anatomy 0.000 description 1
• 201000007295 breast benign neoplasm Diseases 0.000 description 1
• 230000003139 buffering effect Effects 0.000 description 1
• 229910052791 calcium Inorganic materials 0.000 description 1
• 239000001110 calcium chloride Substances 0.000 description 1
• 229910001628 calcium chloride Inorganic materials 0.000 description 1
• 235000011148 calcium chloride Nutrition 0.000 description 1
• 238000004364 calculation method Methods 0.000 description 1
• 239000000969 carrier Substances 0.000 description 1
• 230000015556 catabolic process Effects 0.000 description 1
• 230000003197 catalytic effect Effects 0.000 description 1
• 150000001768 cations Chemical class 0.000 description 1
• 230000001364 causal effect Effects 0.000 description 1
• 230000021164 cell adhesion Effects 0.000 description 1
• 230000006369 cell cycle progression Effects 0.000 description 1
• 230000011712 cell development Effects 0.000 description 1
• 230000024245 cell differentiation Effects 0.000 description 1
• 230000010261 cell growth Effects 0.000 description 1
• 230000004709 cell invasion Effects 0.000 description 1
• 230000009087 cell motility Effects 0.000 description 1
• 230000007248 cellular mechanism Effects 0.000 description 1
• 230000036755 cellular response Effects 0.000 description 1
• 230000005754 cellular signaling Effects 0.000 description 1
• 230000004700 cellular uptake Effects 0.000 description 1
• 229940106157 cellulase Drugs 0.000 description 1
• 238000005119 centrifugation Methods 0.000 description 1
• 210000003756 cervix mucus Anatomy 0.000 description 1
• 238000012512 characterization method Methods 0.000 description 1
• 125000003636 chemical group Chemical group 0.000 description 1
• 235000012000 cholesterol Nutrition 0.000 description 1
• 238000010367 cloning Methods 0.000 description 1
• 229920001436 collagen Polymers 0.000 description 1
• 239000002299 complementary DNA Substances 0.000 description 1
• 230000009918 complex formation Effects 0.000 description 1
• 238000004590 computer program Methods 0.000 description 1
• 230000003750 conditioning effect Effects 0.000 description 1
• 238000012790 confirmation Methods 0.000 description 1
• 238000010276 construction Methods 0.000 description 1
• 238000013270 controlled release Methods 0.000 description 1
• 230000002596 correlated effect Effects 0.000 description 1
• 210000004748 cultured cell Anatomy 0.000 description 1
• 230000002559 cytogenic effect Effects 0.000 description 1
• 210000004292 cytoskeleton Anatomy 0.000 description 1
• 230000001085 cytostatic effect Effects 0.000 description 1
• 231100000433 cytotoxic Toxicity 0.000 description 1
• 230000001472 cytotoxic effect Effects 0.000 description 1
• 230000003013 cytotoxicity Effects 0.000 description 1
• 231100000135 cytotoxicity Toxicity 0.000 description 1
• 230000006378 damage Effects 0.000 description 1
• 230000007423 decrease Effects 0.000 description 1
• 230000003111 delayed effect Effects 0.000 description 1
• 238000004925 denaturation Methods 0.000 description 1
• 230000036425 denaturation Effects 0.000 description 1
• 239000005547 deoxyribonucleotide Substances 0.000 description 1
• 125000002637 deoxyribonucleotide group Chemical group 0.000 description 1
• 230000005595 deprotonation Effects 0.000 description 1
• 238000010537 deprotonation reaction Methods 0.000 description 1
• 238000011161 development Methods 0.000 description 1
• 206010012601 diabetes mellitus Diseases 0.000 description 1
• 238000000502 dialysis Methods 0.000 description 1
• 235000005911 diet Nutrition 0.000 description 1
• 230000037213 diet Effects 0.000 description 1
• MTHSVFCYNBDYFN-UHFFFAOYSA-N diethylene glycol Chemical compound OCCOCCO MTHSVFCYNBDYFN-UHFFFAOYSA-N 0.000 description 1
• 229940042399 direct acting antivirals protease inhibitors Drugs 0.000 description 1
• 230000005750 disease progression Effects 0.000 description 1
• 238000012377 drug delivery Methods 0.000 description 1
• 238000009510 drug design Methods 0.000 description 1
• 238000009509 drug development Methods 0.000 description 1
• 238000007876 drug discovery Methods 0.000 description 1
• 230000000816 effect on animals Effects 0.000 description 1
• 230000002900 effect on cell Effects 0.000 description 1
• 230000005684 electric field Effects 0.000 description 1
• 230000009881 electrostatic interaction Effects 0.000 description 1
• 239000012149 elution buffer Substances 0.000 description 1
• 230000003511 endothelial effect Effects 0.000 description 1
• 210000000981 epithelium Anatomy 0.000 description 1
• 238000011156 evaluation Methods 0.000 description 1
• 239000011536 extraction buffer Substances 0.000 description 1
• 239000012894 fetal calf serum Substances 0.000 description 1
• 229940126864 fibroblast growth factor Drugs 0.000 description 1
• 238000001215 fluorescent labelling Methods 0.000 description 1
• 125000001153 fluoro group Chemical group F* 0.000 description 1
• 238000004108 freeze drying Methods 0.000 description 1
• 125000000524 functional group Chemical group 0.000 description 1
• 230000002538 fungal effect Effects 0.000 description 1
• 230000004927 fusion Effects 0.000 description 1
• 239000008273 gelatin Substances 0.000 description 1
• 229920000159 gelatin Polymers 0.000 description 1
• 235000019322 gelatine Nutrition 0.000 description 1
• 235000011852 gelatine desserts Nutrition 0.000 description 1
• 230000009368 gene silencing by RNA Effects 0.000 description 1
• 230000008303 genetic mechanism Effects 0.000 description 1
• 239000011521 glass Substances 0.000 description 1
• ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
• 125000000404 glutamine group Chemical group N[C@@H](CCC(N)=O)C(=O)* 0.000 description 1
• 108010078144 glutaminyl-glycine Proteins 0.000 description 1
• 229940096919 glycogen Drugs 0.000 description 1
• 239000001963 growth medium Substances 0.000 description 1
• 210000002216 heart Anatomy 0.000 description 1
• 238000010438 heat treatment Methods 0.000 description 1
• 235000014304 histidine Nutrition 0.000 description 1
• 102000051196 human CFAP410 Human genes 0.000 description 1
• 102000056688 human ENTR1 Human genes 0.000 description 1
• 102000048703 human PRELP Human genes 0.000 description 1
• 102000051381 human SLC4A2 Human genes 0.000 description 1
• 102000054983 human ZBTB24 Human genes 0.000 description 1
• 210000005260 human cell Anatomy 0.000 description 1
• 125000004435 hydrogen atom Chemical group [H]* 0.000 description 1
• 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
• 206010020718 hyperplasia Diseases 0.000 description 1
• 238000009802 hysterectomy Methods 0.000 description 1
• 230000028993 immune response Effects 0.000 description 1
• 230000002163 immunogen Effects 0.000 description 1
• 230000001771 impaired effect Effects 0.000 description 1
• 230000008676 import Effects 0.000 description 1
• 230000006882 induction of apoptosis Effects 0.000 description 1
• 230000007574 infarction Effects 0.000 description 1
• 230000008595 infiltration Effects 0.000 description 1
• 238000001764 infiltration Methods 0.000 description 1
• 230000004054 inflammatory process Effects 0.000 description 1
• 230000002401 inhibitory effect Effects 0.000 description 1
• 230000000977 initiatory effect Effects 0.000 description 1
• 230000010354 integration Effects 0.000 description 1
• 230000008611 intercellular interaction Effects 0.000 description 1
• 210000003963 intermediate filament Anatomy 0.000 description 1
• 238000001990 intravenous administration Methods 0.000 description 1
• 238000010253 intravenous injection Methods 0.000 description 1
• 230000002427 irreversible effect Effects 0.000 description 1
• 230000003902 lesion Effects 0.000 description 1
• 230000000670 limiting effect Effects 0.000 description 1
• 239000007788 liquid Substances 0.000 description 1
• 238000011068 loading method Methods 0.000 description 1
• 230000004807 localization Effects 0.000 description 1
• 201000005296 lung carcinoma Diseases 0.000 description 1
• 230000001926 lymphatic effect Effects 0.000 description 1
• 208000003747 lymphoid leukemia Diseases 0.000 description 1
• 108010054155 lysyllysine Proteins 0.000 description 1
• 229920002521 macromolecule Polymers 0.000 description 1
• 229910052749 magnesium Inorganic materials 0.000 description 1
• 230000036212 malign transformation Effects 0.000 description 1
• 230000003211 malignant effect Effects 0.000 description 1
• 208000015486 malignant pancreatic neoplasm Diseases 0.000 description 1
• 201000001441 melanoma Diseases 0.000 description 1
• 230000009061 membrane transport Effects 0.000 description 1
• HOVAGTYPODGVJG-UHFFFAOYSA-N methyl beta-galactoside Natural products COC1OC(CO)C(O)C(O)C1O HOVAGTYPODGVJG-UHFFFAOYSA-N 0.000 description 1
• 210000003632 microfilament Anatomy 0.000 description 1
• 239000004005 microsphere Substances 0.000 description 1
• 210000003470 mitochondria Anatomy 0.000 description 1
• 238000002156 mixing Methods 0.000 description 1
• 102000035118 modified proteins Human genes 0.000 description 1
• 108091005573 modified proteins Proteins 0.000 description 1
• 230000004899 motility Effects 0.000 description 1
• 238000010172 mouse model Methods 0.000 description 1
• 208000025113 myeloid leukemia Diseases 0.000 description 1
• 208000009091 myxoma Diseases 0.000 description 1
• UPSFMJHZUCSEHU-JYGUBCOQSA-N n-[(2s,3r,4r,5s,6r)-2-[(2r,3s,4r,5r,6s)-5-acetamido-4-hydroxy-2-(hydroxymethyl)-6-(4-methyl-2-oxochromen-7-yl)oxyoxan-3-yl]oxy-4,5-dihydroxy-6-(hydroxymethyl)oxan-3-yl]acetamide Chemical compound CC(=O)N[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@H]1[C@H](O)[C@@H](NC(C)=O)[C@H](OC=2C=C3OC(=O)C=C(C)C3=CC=2)O[C@@H]1CO UPSFMJHZUCSEHU-JYGUBCOQSA-N 0.000 description 1
• 230000001338 necrotic effect Effects 0.000 description 1
• 230000002914 neoplasic effect Effects 0.000 description 1
• 230000009826 neoplastic cell growth Effects 0.000 description 1
• 229910052759 nickel Inorganic materials 0.000 description 1
• 229920001220 nitrocellulos Polymers 0.000 description 1
• 230000009871 nonspecific binding Effects 0.000 description 1
• 231100000252 nontoxic Toxicity 0.000 description 1
• 230000003000 nontoxic effect Effects 0.000 description 1
• 108091008104 nucleic acid aptamers Proteins 0.000 description 1
• 239000002777 nucleoside Substances 0.000 description 1
• 150000003833 nucleoside derivatives Chemical class 0.000 description 1
• 238000011580 nude mouse model Methods 0.000 description 1
• 229920001542 oligosaccharide Polymers 0.000 description 1
• 230000008520 organization Effects 0.000 description 1
• 201000008968 osteosarcoma Diseases 0.000 description 1
• 201000002528 pancreatic cancer Diseases 0.000 description 1
• 229920002866 paraformaldehyde Polymers 0.000 description 1
• 230000007918 pathogenicity Effects 0.000 description 1
• 230000009589 pathological growth Effects 0.000 description 1
• 230000037361 pathway Effects 0.000 description 1
• 239000000863 peptide conjugate Substances 0.000 description 1
• 239000000137 peptide hydrolase inhibitor Substances 0.000 description 1
• 230000035699 permeability Effects 0.000 description 1
• 150000008104 phosphatidylethanolamines Chemical class 0.000 description 1
• 150000004713 phosphodiesters Chemical class 0.000 description 1
• 150000008300 phosphoramidites Chemical class 0.000 description 1
• 239000002504 physiological saline solution Substances 0.000 description 1
• 230000036470 plasma concentration Effects 0.000 description 1
• 229920000729 poly(L-lysine) polymer Polymers 0.000 description 1
• 229920002401 polyacrylamide Polymers 0.000 description 1
• 229920002647 polyamide Polymers 0.000 description 1
• 229920000573 polyethylene Polymers 0.000 description 1
• 239000013641 positive control Substances 0.000 description 1
• 231100000683 possible toxicity Toxicity 0.000 description 1
• 108020001213 potassium channel Proteins 0.000 description 1
• 239000002244 precipitate Substances 0.000 description 1
• 239000002243 precursor Substances 0.000 description 1
• 230000003449 preventive effect Effects 0.000 description 1
• 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
• 108010065320 prolyl-lysyl-glutamyl-lysine Proteins 0.000 description 1
• 238000011321 prophylaxis Methods 0.000 description 1
• 125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
• 238000000751 protein extraction Methods 0.000 description 1
• 239000012268 protein inhibitor Substances 0.000 description 1
• 229940121649 protein inhibitor Drugs 0.000 description 1
• JUJWROOIHBZHMG-UHFFFAOYSA-O pyridinium Chemical compound C1=CC=[NH+]C=C1 JUJWROOIHBZHMG-UHFFFAOYSA-O 0.000 description 1
• 238000000163 radioactive labelling Methods 0.000 description 1
• 239000011535 reaction buffer Substances 0.000 description 1
• 230000009257 reactivity Effects 0.000 description 1
• 230000006798 recombination Effects 0.000 description 1
• 238000005215 recombination Methods 0.000 description 1
• 230000007115 recruitment Effects 0.000 description 1
• 206010038038 rectal cancer Diseases 0.000 description 1
• 108091008146 restriction endonucleases Proteins 0.000 description 1
• 210000001525 retina Anatomy 0.000 description 1
• 239000002336 ribonucleotide Substances 0.000 description 1
• 125000002652 ribonucleotide group Chemical group 0.000 description 1
• 210000003705 ribosome Anatomy 0.000 description 1
• 108091092562 ribozyme Proteins 0.000 description 1
• 229920002477 rna polymer Polymers 0.000 description 1
• 210000003296 saliva Anatomy 0.000 description 1
• 231100000241 scar Toxicity 0.000 description 1
• HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 description 1
• 238000012163 sequencing technique Methods 0.000 description 1
• 210000002966 serum Anatomy 0.000 description 1
• 235000020183 skimmed milk Nutrition 0.000 description 1
• 210000003491 skin Anatomy 0.000 description 1
• 230000015590 smooth muscle cell migration Effects 0.000 description 1
• 210000000329 smooth muscle myocyte Anatomy 0.000 description 1
• 125000006850 spacer group Chemical group 0.000 description 1
• 238000004611 spectroscopical analysis Methods 0.000 description 1
• 235000019698 starch Nutrition 0.000 description 1
• 239000008107 starch Substances 0.000 description 1
• 239000007858 starting material Substances 0.000 description 1
• 238000007619 statistical method Methods 0.000 description 1
• 230000005082 stem growth Effects 0.000 description 1
• 230000000638 stimulation Effects 0.000 description 1
• 238000003860 storage Methods 0.000 description 1
• 235000000346 sugar Nutrition 0.000 description 1
• 150000008163 sugars Chemical class 0.000 description 1
• 230000001629 suppression Effects 0.000 description 1
• 230000008961 swelling Effects 0.000 description 1
• 208000024891 symptom Diseases 0.000 description 1
• 230000009885 systemic effect Effects 0.000 description 1
• 229940124597 therapeutic agent Drugs 0.000 description 1
• 210000003051 thermoreceptor Anatomy 0.000 description 1
• 108091008689 thermoreceptors Proteins 0.000 description 1
• 238000004809 thin layer chromatography Methods 0.000 description 1
• RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 description 1
• 230000000699 topical effect Effects 0.000 description 1
• 231100000419 toxicity Toxicity 0.000 description 1
• 230000001988 toxicity Effects 0.000 description 1
• 230000002103 transcriptional effect Effects 0.000 description 1
• 238000003151 transfection method Methods 0.000 description 1
• 230000007704 transition Effects 0.000 description 1
• 238000002054 transplantation Methods 0.000 description 1
• 210000004881 tumor cell Anatomy 0.000 description 1
• 230000005748 tumor development Effects 0.000 description 1
• 239000000439 tumor marker Substances 0.000 description 1
• 241000712461 unidentified influenza virus Species 0.000 description 1
• 241001430294 unidentified retrovirus Species 0.000 description 1
• 230000003827 upregulation Effects 0.000 description 1
• 229940035893 uracil Drugs 0.000 description 1
• 210000002700 urine Anatomy 0.000 description 1
• 238000010200 validation analysis Methods 0.000 description 1
• 230000003966 vascular damage Effects 0.000 description 1
• 230000006648 viral gene expression Effects 0.000 description 1
• 230000007733 viral latency Effects 0.000 description 1
• 235000021119 whey protein Nutrition 0.000 description 1
• 238000012982 x-ray structure analysis Methods 0.000 description 1
• 239000011701 zinc Substances 0.000 description 1
• 229910052725 zinc Inorganic materials 0.000 description 1