BRPI0317276B8 - método para a preparação de partículas de replicon de alfavírus (arps) - Google Patents
método para a preparação de partículas de replicon de alfavírus (arps)Info
- Publication number
- BRPI0317276B8 BRPI0317276B8 BRPI0317276A BR0317276A BRPI0317276B8 BR PI0317276 B8 BRPI0317276 B8 BR PI0317276B8 BR PI0317276 A BRPI0317276 A BR PI0317276A BR 0317276 A BR0317276 A BR 0317276A BR PI0317276 B8 BRPI0317276 B8 BR PI0317276B8
- Authority
- BR
- Brazil
- Prior art keywords
- particles
- alphavirus replicon
- replicon particles
- chloride
- alphavirus
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/525—Virus
- A61K2039/5256—Virus expressing foreign proteins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/36011—Togaviridae
- C12N2770/36111—Alphavirus, e.g. Sindbis virus, VEE, EEE, WEE, Semliki
- C12N2770/36141—Use of virus, viral particle or viral elements as a vector
- C12N2770/36143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/36011—Togaviridae
- C12N2770/36111—Alphavirus, e.g. Sindbis virus, VEE, EEE, WEE, Semliki
- C12N2770/36151—Methods of production or purification of viral material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/36011—Togaviridae
- C12N2770/36111—Alphavirus, e.g. Sindbis virus, VEE, EEE, WEE, Semliki
- C12N2770/36161—Methods of inactivation or attenuation
- C12N2770/36162—Methods of inactivation or attenuation by genetic engineering
Abstract
partículas de alfavírus e métodos para preparação. a presente invenção refere-se a métodos para a produção de partículas de replicon do alfavírus em altos rendimentos; os rnas do replicon são submetidos à eletroporação em células permissivas, em que as células estão em uma densidade relativamente alta, junto com pelo menos um ácido nucléico auxiliador que fornece as funções necessárias para o empacotamento. após um período de crescimento no meio apropriado, as partículas de replicon do alfavírus são coletadas das superfícies das células em que foram produzidas utilizando uma lavagem com sal em que a concentração de sais é de aproximadamente 0,2 até aproximadamente 5 m de cloreto de sódio, cloreto de cálcio, cloreto de magnésio, cloreto de potássio, acetato de amônio, bicarbonato de amônio, entre outros. após a diluição, se necessário, as partículas podem ser purificadas através de uma técnica cromatográfica adequada.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US43305802P | 2002-12-13 | 2002-12-13 | |
US60/433,058 | 2002-12-13 | ||
PCT/US2003/039725 WO2004055167A2 (en) | 2002-12-13 | 2003-12-12 | Alphavirus particles and methods for preparation |
Publications (3)
Publication Number | Publication Date |
---|---|
BR0317276A BR0317276A (pt) | 2005-11-08 |
BRPI0317276B1 BRPI0317276B1 (pt) | 2020-09-15 |
BRPI0317276B8 true BRPI0317276B8 (pt) | 2021-05-25 |
Family
ID=32595112
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR122019005271-9A BR122019005271B1 (pt) | 2002-12-13 | 2003-12-12 | método para a preparação de partículas de replicon de alfavírus (arps) |
BRPI0317276A BRPI0317276B8 (pt) | 2002-12-13 | 2003-12-12 | método para a preparação de partículas de replicon de alfavírus (arps) |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR122019005271-9A BR122019005271B1 (pt) | 2002-12-13 | 2003-12-12 | método para a preparação de partículas de replicon de alfavírus (arps) |
Country Status (13)
Country | Link |
---|---|
US (1) | US7078218B2 (pt) |
EP (3) | EP1590451B1 (pt) |
JP (2) | JP5079981B2 (pt) |
AU (1) | AU2003297041B2 (pt) |
BR (2) | BR122019005271B1 (pt) |
CA (2) | CA2807515C (pt) |
DK (3) | DK1590451T3 (pt) |
ES (3) | ES2552687T3 (pt) |
HK (1) | HK1082524A1 (pt) |
IL (1) | IL169076A (pt) |
NZ (1) | NZ540657A (pt) |
WO (1) | WO2004055167A2 (pt) |
ZA (1) | ZA200505517B (pt) |
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KR101454842B1 (ko) * | 2003-03-20 | 2014-11-04 | 알파벡스, 인크. | 개선된 알파바이러스 레플리콘 및 헬퍼 구축물 |
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2003
- 2003-12-12 NZ NZ540657A patent/NZ540657A/en not_active IP Right Cessation
- 2003-12-12 CA CA2807515A patent/CA2807515C/en not_active Expired - Lifetime
- 2003-12-12 DK DK03813433.4T patent/DK1590451T3/en active
- 2003-12-12 WO PCT/US2003/039725 patent/WO2004055167A2/en active Application Filing
- 2003-12-12 DK DK17163152.6T patent/DK3246399T3/da active
- 2003-12-12 AU AU2003297041A patent/AU2003297041B2/en not_active Expired
- 2003-12-12 EP EP03813433.4A patent/EP1590451B1/en not_active Expired - Lifetime
- 2003-12-12 ES ES03813433.4T patent/ES2552687T3/es not_active Expired - Lifetime
- 2003-12-12 ES ES10011537.7T patent/ES2630222T3/es not_active Expired - Lifetime
- 2003-12-12 JP JP2004560855A patent/JP5079981B2/ja not_active Expired - Lifetime
- 2003-12-12 CA CA2509979A patent/CA2509979C/en not_active Expired - Lifetime
- 2003-12-12 EP EP10011537.7A patent/EP2290054B1/en not_active Expired - Lifetime
- 2003-12-12 US US10/734,609 patent/US7078218B2/en not_active Expired - Lifetime
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- 2003-12-12 DK DK10011537.7T patent/DK2290054T3/en active
- 2003-12-12 BR BRPI0317276A patent/BRPI0317276B8/pt active IP Right Grant
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