BRPI0016121B1 - dispositivo para citômetro de fluxo, com bocal - Google Patents
dispositivo para citômetro de fluxo, com bocal Download PDFInfo
- Publication number
- BRPI0016121B1 BRPI0016121B1 BRPI0016121A BR0016121A BRPI0016121B1 BR PI0016121 B1 BRPI0016121 B1 BR PI0016121B1 BR PI0016121 A BRPI0016121 A BR PI0016121A BR 0016121 A BR0016121 A BR 0016121A BR PI0016121 B1 BRPI0016121 B1 BR PI0016121B1
- Authority
- BR
- Brazil
- Prior art keywords
- nozzle
- flow cytometer
- torsional
- sample
- cytometer device
- Prior art date
Links
- 230000001133 acceleration Effects 0.000 claims description 48
- 238000002347 injection Methods 0.000 claims description 40
- 239000007924 injection Substances 0.000 claims description 40
- 239000012530 fluid Substances 0.000 claims description 24
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- 239000011248 coating agent Substances 0.000 claims description 16
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- 230000001052 transient effect Effects 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 27
- 230000008569 process Effects 0.000 abstract description 21
- 238000004458 analytical method Methods 0.000 abstract description 6
- 241001465754 Metazoa Species 0.000 abstract description 5
- 230000009027 insemination Effects 0.000 abstract description 5
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- 241000283690 Bos taurus Species 0.000 abstract description 4
- 241000283073 Equus caballus Species 0.000 abstract description 4
- 239000000523 sample Substances 0.000 description 111
- 210000004027 cell Anatomy 0.000 description 67
- 230000008859 change Effects 0.000 description 24
- 239000002245 particle Substances 0.000 description 17
- 238000013461 design Methods 0.000 description 9
- 230000006870 function Effects 0.000 description 7
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- 238000000684 flow cytometry Methods 0.000 description 6
- 238000004163 cytometry Methods 0.000 description 5
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- 210000001766 X chromosome Anatomy 0.000 description 2
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- 238000010586 diagram Methods 0.000 description 2
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- 239000007850 fluorescent dye Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 241000124008 Mammalia Species 0.000 description 1
- 241001417935 Platycephalidae Species 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
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- 241000894007 species Species 0.000 description 1
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1404—Handling flow, e.g. hydrodynamic focusing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1404—Handling flow, e.g. hydrodynamic focusing
- G01N15/1409—Handling samples, e.g. injecting samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1404—Handling flow, e.g. hydrodynamic focusing
- G01N2015/1413—Hydrodynamic focussing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1404—Handling flow, e.g. hydrodynamic focusing
- G01N2015/1415—Control of particle position
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/101666—Particle count or volume standard or control [e.g., platelet count standards, etc.]
Landscapes
- Chemical & Material Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Dispersion Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Sampling And Sample Adjustment (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/454,488 US6263745B1 (en) | 1999-12-03 | 1999-12-03 | Flow cytometer nozzle and flow cytometer sample handling methods |
PCT/US2000/042350 WO2001040765A2 (fr) | 1999-12-03 | 2000-11-29 | Buse pour cytometre a liquide perfectionne et procedes de manipulation d'echantillon pour cytometre a liquide |
Publications (2)
Publication Number | Publication Date |
---|---|
BR0016121A BR0016121A (pt) | 2003-02-25 |
BRPI0016121B1 true BRPI0016121B1 (pt) | 2016-12-20 |
Family
ID=23804813
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BRPI0016121A BRPI0016121B1 (pt) | 1999-12-03 | 2000-11-29 | dispositivo para citômetro de fluxo, com bocal |
Country Status (25)
Country | Link |
---|---|
US (4) | US6263745B1 (fr) |
EP (3) | EP1238261B1 (fr) |
JP (2) | JP5019497B2 (fr) |
KR (3) | KR20070058711A (fr) |
CN (1) | CN1402831A (fr) |
AR (4) | AR026683A1 (fr) |
AT (1) | ATE467113T1 (fr) |
AU (1) | AU783000B2 (fr) |
BR (1) | BRPI0016121B1 (fr) |
CA (3) | CA2822851C (fr) |
DE (1) | DE60044373D1 (fr) |
DK (3) | DK1238261T3 (fr) |
ES (2) | ES2445520T3 (fr) |
GB (1) | GB2372466A (fr) |
HK (1) | HK1143860A1 (fr) |
HU (1) | HUP0300587A2 (fr) |
IL (1) | IL149936A0 (fr) |
MX (1) | MXPA02005488A (fr) |
NO (1) | NO20022536L (fr) |
NZ (1) | NZ519275A (fr) |
PL (1) | PL355812A1 (fr) |
RU (1) | RU2002117447A (fr) |
TW (1) | TW538243B (fr) |
UY (1) | UY26469A1 (fr) |
WO (1) | WO2001040765A2 (fr) |
Families Citing this family (103)
Publication number | Priority date | Publication date | Assignee | Title |
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US6861265B1 (en) * | 1994-10-14 | 2005-03-01 | University Of Washington | Flow cytometer droplet formation system |
ATE298084T1 (de) | 1997-01-31 | 2005-07-15 | Horticulture & Food Res Inst | Optische vorrichtung und methode |
US6149867A (en) * | 1997-12-31 | 2000-11-21 | Xy, Inc. | Sheath fluids and collection systems for sex-specific cytometer sorting of sperm |
PL346010A1 (en) | 1998-07-30 | 2002-01-14 | Xy Inc | Equine system for non-surgical artificial insemination |
US7208265B1 (en) * | 1999-11-24 | 2007-04-24 | Xy, Inc. | Method of cryopreserving selected sperm cells |
US6263745B1 (en) | 1999-12-03 | 2001-07-24 | Xy, Inc. | Flow cytometer nozzle and flow cytometer sample handling methods |
IL152714A (en) | 2000-05-09 | 2014-03-31 | Xy Llc | High-purity spermatozoa populations carrying chromosome-x and chromosome-y |
CN1633259A (zh) * | 2000-06-12 | 2005-06-29 | Xy公司 | 利用分离的带x-染色体和带y-染色体的精子群的综合兽群管理系统 |
US20020118402A1 (en) * | 2000-09-19 | 2002-08-29 | Shaw Timothy C. | Film bridge for digital film scanning system |
US7713687B2 (en) | 2000-11-29 | 2010-05-11 | Xy, Inc. | System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations |
US7094527B2 (en) | 2000-11-29 | 2006-08-22 | Xy, Inc. | System for in-vitro fertilization with spermatozoa separated into X-chromosome and Y-chromosome bearing populations |
WO2002092247A1 (fr) * | 2001-05-17 | 2002-11-21 | Cytomation, Inc. | Cytometre de flux a systeme d'alignement optique automatise actif |
US7048505B2 (en) * | 2002-06-21 | 2006-05-23 | Darko Segota | Method and system for regulating fluid flow over an airfoil or a hydrofoil |
US7296411B2 (en) * | 2002-06-21 | 2007-11-20 | Darko Segota | Method and system for regulating internal fluid flow within an enclosed or semi-enclosed environment |
US20050098685A1 (en) * | 2002-06-21 | 2005-05-12 | Darko Segota | Method and system for regulating pressure and optimizing fluid flow about a fuselage similar body |
US7475853B2 (en) * | 2002-06-21 | 2009-01-13 | Darko Segota | Method and system for regulating external fluid flow over an object's surface, and particularly a wing and diffuser |
EP1545203B1 (fr) * | 2002-08-01 | 2016-10-19 | Xy, Llc | Systeme de separation de cellules spermatiques basse pression |
US8486618B2 (en) | 2002-08-01 | 2013-07-16 | Xy, Llc | Heterogeneous inseminate system |
AU2003265471B2 (en) * | 2002-08-15 | 2009-08-06 | Xy, Llc. | High resolution flow cytometer |
US7169548B2 (en) * | 2002-09-13 | 2007-01-30 | Xy, Inc. | Sperm cell processing and preservation systems |
US7201875B2 (en) * | 2002-09-27 | 2007-04-10 | Becton Dickinson And Company | Fixed mounted sorting cuvette with user replaceable nozzle |
CA2516481A1 (fr) * | 2003-02-18 | 2004-09-02 | Board Of Regents, The University Of Texas System | Focalisation de particules dielectriques |
AU2012200711B2 (en) * | 2003-03-28 | 2012-09-20 | Inguran, Llc | "Method and apparatus for orientating sperm in a fluid stream" |
EP3511693B1 (fr) | 2003-03-28 | 2022-08-24 | Inguran, LLC | Dispositif pour détecter le point de détachement d'un système de production de gouttelettes |
DE602004008241T2 (de) * | 2003-03-28 | 2008-05-08 | Monsanto Technology Llc. | Verfahren zum anfärben von sperma |
AU2004242121B2 (en) | 2003-05-15 | 2010-06-24 | Xy, Llc. | Efficient haploid cell sorting for flow cytometer systems |
NZ530972A (en) * | 2004-02-05 | 2005-04-29 | Embrionics Ltd | A method and apparatus for orientating and selecting cells |
WO2005095590A2 (fr) * | 2004-03-29 | 2005-10-13 | Monsanto Technology Llc | Suspensions a spermatozoides pour le tri de leurs populations selon leur richesse en chromosomes x ou y |
WO2005095960A1 (fr) * | 2004-03-29 | 2005-10-13 | Monsanto Technology Llc | Utilisation de composition qui regule des reactions d'oxydation/reduction de maniere intracellulaire et/ou extracellulaire dans un processus de coloration ou de tri de spermatozoides |
DE102005052752A1 (de) * | 2005-11-04 | 2007-05-10 | Clondiag Chip Technologies Gmbh | Vorrichtung und Verfahren zum Nachweis von molekularen Wechselwirkungen |
CA2574499C (fr) | 2004-07-22 | 2016-11-29 | Monsanto Technology Llc | Procede pour enrichir une population de spermatozoides |
US7340957B2 (en) | 2004-07-29 | 2008-03-11 | Los Alamos National Security, Llc | Ultrasonic analyte concentration and application in flow cytometry |
US7355696B2 (en) | 2005-02-01 | 2008-04-08 | Arryx, Inc | Method and apparatus for sorting cells |
FR2883973B1 (fr) * | 2005-03-31 | 2007-11-16 | C2 Diagnostics Sa | Cuve pour dispositif optique d'analyse sanguine, appareil d'analyse equipe d'une telle cuve |
US20070025879A1 (en) * | 2005-07-27 | 2007-02-01 | Dakocytomation Denmark A/S | Method and apparatus for syringe-based sample introduction within a flow cytometer |
US7618770B2 (en) * | 2005-07-29 | 2009-11-17 | Xy, Inc. | Methods and apparatus for reducing protein content in sperm cell extenders |
US7835000B2 (en) | 2006-11-03 | 2010-11-16 | Los Alamos National Security, Llc | System and method for measuring particles in a sample stream of a flow cytometer or the like |
ATE538377T1 (de) | 2007-04-02 | 2012-01-15 | Acoustic Cytometry Systems Inc | Verfahren zur verbesserten analyse von in einem akustischen feld fokussierten zellen und partikeln |
US7837040B2 (en) * | 2007-04-09 | 2010-11-23 | Los Alamos National Security, Llc | Acoustic concentration of particles in fluid flow |
US8083068B2 (en) | 2007-04-09 | 2011-12-27 | Los Alamos National Security, Llc | Apparatus for separating particles utilizing engineered acoustic contrast capture particles |
US8528406B2 (en) | 2007-10-24 | 2013-09-10 | Los Alamos National Security, LLP | Method for non-contact particle manipulation and control of particle spacing along an axis |
US8263407B2 (en) * | 2007-10-24 | 2012-09-11 | Los Alamos National Security, Llc | Method for non-contact particle manipulation and control of particle spacing along an axis |
CA2708751A1 (fr) | 2007-12-14 | 2009-06-25 | Minitube Of America, Inc. | Separation specifique a chaque sexe de spermatozoides et d'embryons |
US8266950B2 (en) | 2007-12-19 | 2012-09-18 | Los Alamos National Security, LLP | Particle analysis in an acoustic cytometer |
US8714014B2 (en) * | 2008-01-16 | 2014-05-06 | Life Technologies Corporation | System and method for acoustic focusing hardware and implementations |
JP4661942B2 (ja) | 2008-05-13 | 2011-03-30 | ソニー株式会社 | マイクロチップとその流路構造 |
CA2727478A1 (fr) * | 2008-06-13 | 2009-12-17 | Xy, Llc | Systeme de trajet d'ecoulement microfluide lubrifiant |
US20100009333A1 (en) * | 2008-07-08 | 2010-01-14 | Beckman Coulter, Inc. | Methods for Acoustic Particle Focusing in Biological Sample Analyzers |
DE102008064667B4 (de) * | 2008-07-15 | 2011-06-09 | Lzh Laserzentrum Hannover E.V. | Verfahren zur Herstellung eines Detektionskonjugats |
JP5487638B2 (ja) | 2009-02-17 | 2014-05-07 | ソニー株式会社 | 微小粒子分取のための装置及びマイクロチップ |
WO2011068764A2 (fr) * | 2009-12-04 | 2011-06-09 | Life Technologies Corporation | Appareils, systèmes, procédés et supports lisibles par ordinateur destinés à la cytométrie de flux acoustique |
US20110236923A1 (en) * | 2010-03-23 | 2011-09-29 | Genetics & Ivf Institute | Method for staining and sorting of a small volume of sperm |
US20130011825A1 (en) | 2010-04-01 | 2013-01-10 | Inguran, Llc | Methods and systems for reducing dna fragmentation in a processed sperm sample |
CA2826544C (fr) | 2011-02-04 | 2020-06-30 | Cytonome/St, Llc | Appareil et procede de triage de particules |
DE102011006080B4 (de) | 2011-03-24 | 2015-06-18 | Masterrind Gmbh | Vorrichtung und Verfahren zur Fraktionierung von Säugerspermatozoen |
DE102011006081A1 (de) | 2011-03-24 | 2012-09-27 | Masterrind Gmbh | Düse zur Ausrichtung eines Flüssigkeitsteilstroms |
DK2707699T3 (da) | 2011-05-12 | 2021-09-20 | Xy Llc | Uv-diodelaserexcitering i flow-cytometri |
DE102011075711A1 (de) | 2011-05-12 | 2012-11-15 | Masterrind Gmbh | Düse zur Partikelausrichtung im Flüssigkeitsstrom |
JP2013024629A (ja) * | 2011-07-19 | 2013-02-04 | Sysmex Corp | フローサイトメータ |
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US9433195B2 (en) | 2012-06-06 | 2016-09-06 | Inguran, Llc | Methods for increasing genetic progress in a line or breed of swine using sex-selected sperm cells |
US9888990B2 (en) | 2012-06-06 | 2018-02-13 | Inguran, Llc | Methods for use of sex sorted semen to improve genetic management in swine |
CN102795668B (zh) * | 2012-09-12 | 2014-07-09 | 西南大学 | 一种vo2的制备方法 |
WO2014047206A1 (fr) * | 2012-09-18 | 2014-03-27 | Cytonome/St, Llc | Cuve à circulation pour le tri de particules |
US11668640B2 (en) | 2015-03-06 | 2023-06-06 | Inguran, Llc | Nozzle assembly for a flow cytometry system and methods of manufacture |
CA3234097A1 (fr) * | 2012-09-19 | 2014-03-27 | Inguran, Llc | Ensemble buse d'un systeme de cytometre de flux et procedes de fabrication |
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EP2903432B1 (fr) | 2012-10-05 | 2019-05-08 | Inguran, LLC | Procédés de traitement de sperme pour un tri par sexe |
US10620213B2 (en) | 2012-10-05 | 2020-04-14 | Inguran, Llc | High pressure sperm sorting and flow cytometer methods |
DE202012105015U1 (de) | 2012-12-21 | 2013-03-05 | Laser Zentrum Hannover E.V. | Einrichtung mit einem inneren und einem äußeren Funktionselement |
JP2014174139A (ja) * | 2013-03-13 | 2014-09-22 | Sony Corp | 流路デバイス、粒子分取装置、粒子流出方法、及び粒子分取方法 |
US9757726B2 (en) | 2013-03-14 | 2017-09-12 | Inguran, Llc | System for high throughput sperm sorting |
US10662408B2 (en) | 2013-03-14 | 2020-05-26 | Inguran, Llc | Methods for high throughput sperm sorting |
AU2013202632B2 (en) * | 2013-03-14 | 2015-04-16 | Inguran, Llc | Apparatus and methods for high throughput sperm sorting |
US10371622B2 (en) | 2013-03-14 | 2019-08-06 | Inguran, Llc | Device for high throughput sperm sorting |
DE102013208584A1 (de) * | 2013-05-08 | 2014-11-13 | Masterrind Gmbh | Düse und Verfahren für die Durchflusszytometrie |
US10870175B2 (en) * | 2013-09-18 | 2020-12-22 | Cytonome/St, Llc | Microfluidic flow-through elements and methods of manufacture of same |
CN103555662B (zh) * | 2013-10-31 | 2015-09-16 | 大连金弘基种畜有限公司 | 将四溴化1,1,2,2-四-[4-(2-三乙基乙氧基胺基)]苯乙烯应用于精子分离 |
JP2015222202A (ja) * | 2014-05-22 | 2015-12-10 | ソニー株式会社 | 粒子分析装置 |
CA2905670A1 (fr) | 2014-09-26 | 2016-03-26 | Inguran, Llc | Sperme trie selon le sexe manifestant une dose-reponse et procedes de production de sperme trie selon le sexe manifestant une dose-reponse |
WO2016090310A1 (fr) | 2014-12-05 | 2016-06-09 | Inguran, Llc | Traitement de cellules à l'aide de particules magnétiques |
WO2016154131A1 (fr) * | 2015-03-23 | 2016-09-29 | New York University | Systèmes et procédés pour sélectionner des souches cellulaires |
WO2017202932A2 (fr) | 2016-05-24 | 2017-11-30 | Cellix Limited | Appareil d'analyse par cytométrie en flux microfluidique d'un fluide contenant des particules |
USD869676S1 (en) | 2017-03-28 | 2019-12-10 | Becton, Dickinson And Company | Particle sorting module |
USD868991S1 (en) | 2017-03-28 | 2019-12-03 | Becton, Dickinson And Company | Register block |
FR3068469B1 (fr) * | 2017-06-28 | 2020-09-11 | Diagdev | Cuve de mesure pour le denombrement et/ou la caracterisation de cellules |
CN110945343B (zh) | 2017-07-19 | 2023-01-13 | 英格朗公司 | 分析仪器和产生精子群体的方法 |
USD872296S1 (en) | 2018-01-30 | 2020-01-07 | Becton, Dickinson And Company | Particle sorting module |
USD882817S1 (en) | 2018-01-30 | 2020-04-28 | Becton, Dickinson And Company | Sample container |
USD864415S1 (en) | 2018-01-30 | 2019-10-22 | Becton, Dickinson And Company | Particle sorting system |
USD876668S1 (en) | 2018-01-30 | 2020-02-25 | Becton, Dickinson And Company | Particle sorting module mount |
EP3772937A4 (fr) | 2018-04-09 | 2021-12-29 | Inguran, LLC | Noyaux de sperme améliorés et procédés d'obtention et d'utilisation de ces derniers |
CN117085750A (zh) * | 2018-04-25 | 2023-11-21 | 产生技术有限公司 | 与微流体系统相关联的系统、设备和方法 |
CA3133622C (fr) | 2019-03-19 | 2024-04-23 | Inguran, Llc | Procede pour des populations de cellules spermatiques ameliorees |
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