BR9908422A - Pululanase modificada, ácido nucleico, vetor de expressão, microorganismo hospedeiro, processo para a produção de uma pululanase modificada em uma célula hospedeira, composição enzimática, processo para sacarificação de amido, e, b. licheniformis - Google Patents

Pululanase modificada, ácido nucleico, vetor de expressão, microorganismo hospedeiro, processo para a produção de uma pululanase modificada em uma célula hospedeira, composição enzimática, processo para sacarificação de amido, e, b. licheniformis

Info

Publication number
BR9908422A
BR9908422A BR9908422-8A BR9908422A BR9908422A BR 9908422 A BR9908422 A BR 9908422A BR 9908422 A BR9908422 A BR 9908422A BR 9908422 A BR9908422 A BR 9908422A
Authority
BR
Brazil
Prior art keywords
modified
modified pullulanase
nucleic acid
production
pullulanase
Prior art date
Application number
BR9908422-8A
Other languages
English (en)
Inventor
Brian S Miller
Jayarama K Shetty
Original Assignee
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int filed Critical Genencor Int
Publication of BR9908422A publication Critical patent/BR9908422A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C12N9/2457—Pullulanase (3.2.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C12N9/246—Isoamylase (3.2.1.68)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01041—Pullulanase (3.2.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01068—Isoamylase (3.2.1.68)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

"PULULANASE MODIFICADA, áCIDO NUCLEICO, VETOR DE EXPRESSãO, MICROORGANISMO HOSPEDEIRO, PROCESSO PARA A PRODUçãO DE UMA PULULANASE MODIFICADA EM UMA CéLULA HOSPEDEIRA, COMPOSIçãO ENZIMáTICA, PROCESSO PARA SACARIFICAçãO DE AMIDO, E, B.LICHENIFORMIS". A presente invenção refere-se às pululanases modificadas úteis na indústria de amido. A presente invenção proporciona processos para produção de pululanase modificada, composições enzimáticas compreendendo pululanase modificada, e processos para a sacarificação de amido compreendendo o emprego de composições enzimáticas.
BR9908422-8A 1998-03-04 1999-03-03 Pululanase modificada, ácido nucleico, vetor de expressão, microorganismo hospedeiro, processo para a produção de uma pululanase modificada em uma célula hospedeira, composição enzimática, processo para sacarificação de amido, e, b. licheniformis BR9908422A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US3463098A 1998-03-04 1998-03-04
PCT/US1999/004627 WO1999045124A2 (en) 1998-03-04 1999-03-03 Modified forms of pullulanase

Publications (1)

Publication Number Publication Date
BR9908422A true BR9908422A (pt) 2000-10-31

Family

ID=21877610

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9908422-8A BR9908422A (pt) 1998-03-04 1999-03-03 Pululanase modificada, ácido nucleico, vetor de expressão, microorganismo hospedeiro, processo para a produção de uma pululanase modificada em uma célula hospedeira, composição enzimática, processo para sacarificação de amido, e, b. licheniformis

Country Status (9)

Country Link
US (2) US7449320B2 (pt)
EP (1) EP1060253A2 (pt)
JP (1) JP2002505108A (pt)
CN (1) CN1249226C (pt)
AU (1) AU2980199A (pt)
BR (1) BR9908422A (pt)
CA (1) CA2321817C (pt)
NZ (1) NZ527414A (pt)
WO (1) WO1999045124A2 (pt)

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US20080193982A1 (en) * 2005-05-19 2008-08-14 Novozymes Biotech, Inc. Production of Enzymes
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US8168181B2 (en) 2006-02-13 2012-05-01 Alethia Biotherapeutics, Inc. Methods of impairing osteoclast differentiation using antibodies that bind siglec-15
US8765199B2 (en) 2006-06-15 2014-07-01 Novozymes A/S Mashing process
US9969996B2 (en) 2006-08-04 2018-05-15 Amano Enzyme Inc. Method for making mutated pullulanase enzyme, mutated pullulanase enzyme, and microorganism expressing the same
JP5178517B2 (ja) 2006-08-04 2013-04-10 天野エンザイム株式会社 変異型酵素の設計法、調製法、及び変異型酵素
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US8175068B2 (en) * 2008-03-26 2012-05-08 Advanced Receiver Technologies, Llc Active spread code detection
US8194138B2 (en) * 2008-12-17 2012-06-05 Getac Technology Corporation Portable electronic device and camera module thereof
US20100159777A1 (en) * 2008-12-18 2010-06-24 Kimberly-Clark Worldwide, Inc. Thermoplastic Starch Formed from an Enzymatically Debranched Starch
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CN102409032B (zh) * 2011-10-14 2013-06-26 江南大学 一种化学修饰的生麦芽糖淀粉酶
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CN103242996B (zh) * 2013-04-25 2014-04-09 湖南鸿鹰生物科技有限公司 一种啤酒复合酶
CN103571812B (zh) * 2013-11-26 2015-06-24 江南大学 一种分泌效率和热稳定性提高的普鲁兰酶突变体及其制备方法
AU2015369965B2 (en) 2014-12-23 2020-01-30 Nutrition & Biosciences USA 4, Inc. Enzymatically produced cellulose
CN107532155B (zh) * 2015-02-04 2019-06-25 南京百斯杰生物工程有限公司 截短普鲁兰酶及其生产方法和应用方法
CN105734034B (zh) * 2016-04-25 2019-05-10 江南大学 利用截短柔性残基提高普鲁兰酶催化性能的方法
CN109576255B (zh) * 2018-12-19 2022-07-26 广州医科大学 一种酶的防腐剂及其使用方法
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Also Published As

Publication number Publication date
CA2321817A1 (en) 1999-09-10
NZ527414A (en) 2005-07-29
WO1999045124A2 (en) 1999-09-10
CA2321817C (en) 2011-02-01
EP1060253A2 (en) 2000-12-20
US7449320B2 (en) 2008-11-11
AU2980199A (en) 1999-09-20
CN1249226C (zh) 2006-04-05
US7399623B2 (en) 2008-07-15
CN1292030A (zh) 2001-04-18
WO1999045124A3 (en) 1999-11-18
JP2002505108A (ja) 2002-02-19
US20030013180A1 (en) 2003-01-16
US20030022348A1 (en) 2003-01-30

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Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTA A 6O, 7O E 8O ANUIDADES.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 1911 DE 21/08/2007.