BR9908422A - Pululanase modificada, ácido nucleico, vetor de expressão, microorganismo hospedeiro, processo para a produção de uma pululanase modificada em uma célula hospedeira, composição enzimática, processo para sacarificação de amido, e, b. licheniformis - Google Patents

Pululanase modificada, ácido nucleico, vetor de expressão, microorganismo hospedeiro, processo para a produção de uma pululanase modificada em uma célula hospedeira, composição enzimática, processo para sacarificação de amido, e, b. licheniformis

Info

Publication number
BR9908422A
BR9908422A BR9908422-8A BR9908422A BR9908422A BR 9908422 A BR9908422 A BR 9908422A BR 9908422 A BR9908422 A BR 9908422A BR 9908422 A BR9908422 A BR 9908422A
Authority
BR
Brazil
Prior art keywords
modified
modified pullulanase
nucleic acid
production
pullulanase
Prior art date
Application number
BR9908422-8A
Other languages
English (en)
Inventor
Brian S Miller
Jayarama K Shetty
Original Assignee
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int filed Critical Genencor Int
Publication of BR9908422A publication Critical patent/BR9908422A/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C12N9/2457—Pullulanase (3.2.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C12N9/246—Isoamylase (3.2.1.68)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01041—Pullulanase (3.2.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01068—Isoamylase (3.2.1.68)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

"PULULANASE MODIFICADA, áCIDO NUCLEICO, VETOR DE EXPRESSãO, MICROORGANISMO HOSPEDEIRO, PROCESSO PARA A PRODUçãO DE UMA PULULANASE MODIFICADA EM UMA CéLULA HOSPEDEIRA, COMPOSIçãO ENZIMáTICA, PROCESSO PARA SACARIFICAçãO DE AMIDO, E, B.LICHENIFORMIS". A presente invenção refere-se às pululanases modificadas úteis na indústria de amido. A presente invenção proporciona processos para produção de pululanase modificada, composições enzimáticas compreendendo pululanase modificada, e processos para a sacarificação de amido compreendendo o emprego de composições enzimáticas.
BR9908422-8A 1998-03-04 1999-03-03 Pululanase modificada, ácido nucleico, vetor de expressão, microorganismo hospedeiro, processo para a produção de uma pululanase modificada em uma célula hospedeira, composição enzimática, processo para sacarificação de amido, e, b. licheniformis BR9908422A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US3463098A 1998-03-04 1998-03-04
PCT/US1999/004627 WO1999045124A2 (en) 1998-03-04 1999-03-03 Modified forms of pullulanase

Publications (1)

Publication Number Publication Date
BR9908422A true BR9908422A (pt) 2000-10-31

Family

ID=21877610

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9908422-8A BR9908422A (pt) 1998-03-04 1999-03-03 Pululanase modificada, ácido nucleico, vetor de expressão, microorganismo hospedeiro, processo para a produção de uma pululanase modificada em uma célula hospedeira, composição enzimática, processo para sacarificação de amido, e, b. licheniformis

Country Status (9)

Country Link
US (2) US7449320B2 (pt)
EP (1) EP1060253A2 (pt)
JP (1) JP2002505108A (pt)
CN (1) CN1249226C (pt)
AU (1) AU2980199A (pt)
BR (1) BR9908422A (pt)
CA (1) CA2321817C (pt)
NZ (1) NZ527414A (pt)
WO (1) WO1999045124A2 (pt)

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DK1994155T4 (da) 2006-02-13 2022-07-25 Daiichi Sankyo Co Ltd Polynukleotid- og polypeptidsekvenser involveret i fremgangsmåden med knogleremodellering
US8168181B2 (en) 2006-02-13 2012-05-01 Alethia Biotherapeutics, Inc. Methods of impairing osteoclast differentiation using antibodies that bind siglec-15
US8765199B2 (en) 2006-06-15 2014-07-01 Novozymes A/S Mashing process
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US9969996B2 (en) 2006-08-04 2018-05-15 Amano Enzyme Inc. Method for making mutated pullulanase enzyme, mutated pullulanase enzyme, and microorganism expressing the same
WO2008015861A1 (fr) * 2006-08-04 2008-02-07 Amano Enzyme Inc. Procédé de conception d'enzyme mutée, son procédé de préparation et enzyme mutée
US7968691B2 (en) * 2006-08-23 2011-06-28 Danisco Us Inc. Pullulanase variants with increased productivity
BRPI0819869B1 (pt) 2007-12-12 2019-07-16 Novozymes A/S Processo enzimático para a produção de um mosto de cervejeiro a partir de cereal não maltado, e, uso de um processo para produção de cerveja
US8175068B2 (en) * 2008-03-26 2012-05-08 Advanced Receiver Technologies, Llc Active spread code detection
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US20100159777A1 (en) * 2008-12-18 2010-06-24 Kimberly-Clark Worldwide, Inc. Thermoplastic Starch Formed from an Enzymatically Debranched Starch
EP2430176A2 (en) * 2009-05-11 2012-03-21 Danisco US Inc. Improved production of maltotetraose syrup using a pseudomonas saccharophila maltotetraohydrolase variant and a debranching enzyme
MX2012001243A (es) 2009-08-07 2012-03-26 Danisco Us Inc Combinacion de alfa-amilasa para procesamiento de almidon y metodo de uso de la misma.
CN102409032B (zh) * 2011-10-14 2013-06-26 江南大学 一种化学修饰的生麦芽糖淀粉酶
AU2013293062A1 (en) 2012-07-19 2014-07-24 Alethia Biotherapeutics Inc. Anti-Siglec-15 antibodies
CN103242996B (zh) * 2013-04-25 2014-04-09 湖南鸿鹰生物科技有限公司 一种啤酒复合酶
CN103571812B (zh) * 2013-11-26 2015-06-24 江南大学 一种分泌效率和热稳定性提高的普鲁兰酶突变体及其制备方法
CA2969241A1 (en) 2014-12-23 2016-06-30 E.I. Du Pont De Nemours And Company Enzymatically produced cellulose
EP3253869A4 (en) * 2015-02-04 2018-11-07 Nanjing Bestzyme Bio-Engineering Co. Ltd. Truncated pullulanases, methods of production, and methods of use thereof
CN105734034B (zh) * 2016-04-25 2019-05-10 江南大学 利用截短柔性残基提高普鲁兰酶催化性能的方法
CN109576255B (zh) * 2018-12-19 2022-07-26 广州医科大学 一种酶的防腐剂及其使用方法
EP4347812A1 (en) 2021-05-24 2024-04-10 Danisco US Inc. Compositions and methods for enhanced protein production in bacillus cells
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Also Published As

Publication number Publication date
WO1999045124A3 (en) 1999-11-18
CN1292030A (zh) 2001-04-18
US20030022348A1 (en) 2003-01-30
CA2321817C (en) 2011-02-01
NZ527414A (en) 2005-07-29
US7399623B2 (en) 2008-07-15
JP2002505108A (ja) 2002-02-19
CN1249226C (zh) 2006-04-05
CA2321817A1 (en) 1999-09-10
EP1060253A2 (en) 2000-12-20
AU2980199A (en) 1999-09-20
US20030013180A1 (en) 2003-01-16
WO1999045124A2 (en) 1999-09-10
US7449320B2 (en) 2008-11-11

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Legal Events

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B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTA A 6O, 7O E 8O ANUIDADES.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 1911 DE 21/08/2007.