BR0010998A - Enzima, molécula de polinucleotìdeo isolado, vetor de expressão, célula cultivada em que tenha sido introduzido um vetor de expressão, composição enzimática, cultura biológica isolada, e, processos para degradação de biomassa contendo celulose, e para produção de um polipeptìdeo - Google Patents

Enzima, molécula de polinucleotìdeo isolado, vetor de expressão, célula cultivada em que tenha sido introduzido um vetor de expressão, composição enzimática, cultura biológica isolada, e, processos para degradação de biomassa contendo celulose, e para produção de um polipeptìdeo

Info

Publication number
BR0010998A
BR0010998A BR0010998-3A BR0010998A BR0010998A BR 0010998 A BR0010998 A BR 0010998A BR 0010998 A BR0010998 A BR 0010998A BR 0010998 A BR0010998 A BR 0010998A
Authority
BR
Brazil
Prior art keywords
enzyme
expression vector
nucleotide
amino acid
polypeptide
Prior art date
Application number
BR0010998-3A
Other languages
English (en)
Inventor
Martin Schuelein
Mads Eskeland Bjornvard
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Publication of BR0010998A publication Critical patent/BR0010998A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

"ENZIMA, MOLéCULA DE POLINUCLEOTìDEO ISOLADO VETOR DE EXPRESSãO, CéLULA CULTIVADA EM QUE TENHA SIDO INTRODUZIDO UM VETOR DE EXPRESSãO, COMPOSIçãO ENZIMáTICA, CULTURA BIOLóGICA ISOLADA, E, PROCESSOS PARA DEGRADAçãO DE BIOMASSA CONTENDO CELULOSE, E PARA PRODUçãO DE UM POLIPEPTìDEO". Uma enzima apresentando atividade de endo-<225>-1,4-glucanase, que pertence à familia 9 das glicosil hidrolases, é obtenível de, ou endógena a, uma cepa pertencente ao gênero Bacillus, tal como Bacillus licheniformis, ATCC 14580; uma Molécula de polinucleodideo isolado (DNA) codificando uma enzima ou núcleo enzimático (o domínio cataliticamente ativo da enzima) apresentando atividade de endo-<225>-1,4-glucanase selecionada de (a) moléculas de polinucleotideos compreendendo uma seq³ência de nucleotídeos como apresentado na SEQ ID NO: 1 do nucleotídeo 76 ao nucleotídeo 1455 ou do nucleotídeo 76 ao nucleotídeo 1941, (b) moléculas de polinucleotídeos que codificam um polipeptídeo sendo pelo menos 75% idêntico à seq³ência de aminoácido da SEQ ID NO: 2 do resíduo de aminoácido 26 ao aminoácido 485 ou do residuo de aminoácido 26 ao resíduo de aminoácido 646, e (c) seq³ências degeneradas de nucleotídeos de (a) ou (b), a enzima endoglucanase expressa sendo útil em várias aplicações industriais.
BR0010998-3A 1999-05-28 2000-05-24 Enzima, molécula de polinucleotìdeo isolado, vetor de expressão, célula cultivada em que tenha sido introduzido um vetor de expressão, composição enzimática, cultura biológica isolada, e, processos para degradação de biomassa contendo celulose, e para produção de um polipeptìdeo BR0010998A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA199900755 1999-05-28
PCT/DK2000/000278 WO2000073428A1 (en) 1999-05-28 2000-05-24 Novel endo-beta-1,4-glucanases

Publications (1)

Publication Number Publication Date
BR0010998A true BR0010998A (pt) 2002-06-04

Family

ID=8097172

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0010998-3A BR0010998A (pt) 1999-05-28 2000-05-24 Enzima, molécula de polinucleotìdeo isolado, vetor de expressão, célula cultivada em que tenha sido introduzido um vetor de expressão, composição enzimática, cultura biológica isolada, e, processos para degradação de biomassa contendo celulose, e para produção de um polipeptìdeo

Country Status (11)

Country Link
EP (1) EP1185631B9 (pt)
JP (1) JP4837855B2 (pt)
KR (1) KR20020012249A (pt)
CN (1) CN1177929C (pt)
AT (1) ATE335812T1 (pt)
AU (1) AU4744900A (pt)
BR (1) BR0010998A (pt)
CA (1) CA2374433A1 (pt)
DE (2) DE60029955T4 (pt)
MX (1) MXPA01012179A (pt)
WO (1) WO2000073428A1 (pt)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7041488B2 (en) 2001-06-06 2006-05-09 Novozymes A/S Endo-beta-1,4-glucanase from bacillus
JP4242761B2 (ja) * 2001-06-06 2009-03-25 ノボザイムス アクティーゼルスカブ エンド−β−１，４−グルカナーゼ
CN1306022C (zh) * 2004-09-13 2007-03-21 山东省科学院生物研究所 巨大芽孢杆菌内切葡聚糖酶的基因及其制备方法
ATE515568T1 (de) * 2005-11-16 2011-07-15 Novozymes As Polypeptide mit endoglucanase-aktivtät und dafür codierende polynukleotide
AU2007356171B8 (en) * 2006-08-04 2014-01-16 Bp Corporation North America Inc. Glucanases, nucleic acids encoding them, and methods for making and using them
CN101215537B (zh) * 2007-12-29 2010-06-16 北京欣博阳科技有限公司 地衣芽孢杆菌及其应用
CN102899340B (zh) * 2012-10-27 2013-09-25 广西大学 编码糖基水解酶家族6的内切葡聚糖酶基因Cel6及其应用
CN102888418B (zh) * 2012-11-01 2013-10-09 广西大学 链霉菌的内切葡聚糖酶基因Cel5B及其应用
CN103484390A (zh) * 2013-09-17 2014-01-01 山东农业大学 表达9家族糖苷水解酶基因CtAA9b的毕赤酵母工程菌株
CN103509727A (zh) * 2013-09-17 2014-01-15 山东农业大学 表达9家族糖苷水解酶基因CtAA9c的毕赤酵母工程菌株
CN103436456A (zh) * 2013-09-17 2013-12-11 山东农业大学 表达9家族糖苷水解酶基因CtAA9a的毕赤酵母工程菌株
CN103642774B (zh) * 2013-11-13 2016-08-17 宁夏夏盛实业集团有限公司 混合中性纤维素酶及其制备方法和在造纸打浆中的应用
FI3344814T3 (fi) * 2015-09-03 2023-10-13 Solenis Tech Lp Menetelmä lignoselluloosapaperin ja -paperituotteiden valmistamiseksi
CN105624138B (zh) * 2016-02-04 2019-01-29 嘉兴卓盛生物科技有限公司 一种生活用纸打浆促进纤维改性的复合酶制剂及其制备方法和应用
KR102375732B1 (ko) * 2017-02-24 2022-03-16 다니스코 유에스 인크. 바실러스 리체니포르미스에서 단백질 생산을 증가시키기 위한 조성물 및 방법
CN107974442B (zh) * 2017-12-18 2019-10-22 浙江大学 内切葡聚糖酶、其编码基因cel5A-h42及其应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0270974B1 (en) * 1986-12-05 1995-04-26 Kao Corporation Alkali-resistant cellulases and microorganisms capable of producing same
DK16490D0 (da) * 1990-01-19 1990-01-19 Novo Nordisk As Enzym
AU1946895A (en) * 1994-03-28 1995-10-17 Novo Nordisk A/S A modified cellulase and an enzyme preparation comprising a modified cellulase

Also Published As

Publication number Publication date
CN1177929C (zh) 2004-12-01
CN1353751A (zh) 2002-06-12
JP2003501021A (ja) 2003-01-14
KR20020012249A (ko) 2002-02-15
JP4837855B2 (ja) 2011-12-14
MXPA01012179A (es) 2002-07-22
CA2374433A1 (en) 2000-12-07
DE60029955T2 (de) 2007-09-06
DE60029955T4 (de) 2007-12-27
EP1185631A1 (en) 2002-03-13
EP1185631B1 (en) 2006-08-09
ATE335812T1 (de) 2006-09-15
AU4744900A (en) 2000-12-18
EP1185631B9 (en) 2007-02-28
WO2000073428A1 (en) 2000-12-07
DE60029955D1 (de) 2006-09-21

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B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]