BR9810402A - Engenheiramento de tioesterases de planta e divulgação de tioesterases de planta tendo especificidade de substrato - Google Patents

Engenheiramento de tioesterases de planta e divulgação de tioesterases de planta tendo especificidade de substrato

Info

Publication number
BR9810402A
BR9810402A BR9810402-0A BR9810402A BR9810402A BR 9810402 A BR9810402 A BR 9810402A BR 9810402 A BR9810402 A BR 9810402A BR 9810402 A BR9810402 A BR 9810402A
Authority
BR
Brazil
Prior art keywords
plant
thioesterases
thioesterase
engineering
activity
Prior art date
Application number
BR9810402-0A
Other languages
English (en)
Inventor
Ling Yuan
Original Assignee
Calgene Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Calgene Llc filed Critical Calgene Llc
Publication of BR9810402A publication Critical patent/BR9810402A/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Patente de Invenção: <B>"ENGENHEIRAMENTO DE TIOESTERASES DE PLANTA E DIVULGAçãO DE TIOESTERASES DE PLANTA TENDO ESPECIFICIDADE DE SUBSTRATO''<D>. Métodos de alterar a especificidade do substrato de tioesterases de acil-ACP de planta, e tioesterases de acil-ACP de planta construída assim produzidas são fornecidos. A porção de dois terços de terminal C de tioesterases de planta é identificada como desejável para tais modificações. As seq³ências e construções e DNA para a expressão de tioesterases construídas, bem como as novas tioesterases produzidas delas são também fornecidas. Tais seq³ências de DNA podem ser empregadas para a expressão das tioesterases construídas em células hospedeiras, particularmente células de semente de plantas de colheira de semente oleoginosa, para a modificação de composição de ácido graxo. Um C12 preferindo a tioesterase de acil-ACP de planta descrito aui pode ser alterado para obter uma tioesterase de planta tendo aproximadamente igual atividade sobre os substratos C14 e C12. Outra modificação da enzima C12 produz uma tioesterase tendo maior atividade sobre o C14 quando comprado aos substratos C12. De particular interesse é uma tioesterase 18:1 de planta na qual a atividade 18:0 relativa foi aumentada. Tais tioesterase de FatA encontram uso para a produção melhorada de estearato em óleos de semente vegetal.
BR9810402-0A 1997-06-03 1998-06-03 Engenheiramento de tioesterases de planta e divulgação de tioesterases de planta tendo especificidade de substrato BR9810402A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/868,458 US6150512A (en) 1995-05-15 1997-06-03 Engineering plant thioesterases and disclosure of plant thioesterases having novel substrate specificity
PCT/US1998/011697 WO1998055633A1 (en) 1997-06-03 1998-06-03 Engineering plant thioesterases and disclosure of plant thioesterases having novel substrate specificity

Publications (1)

Publication Number Publication Date
BR9810402A true BR9810402A (pt) 2000-08-29

Family

ID=25351727

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9810402-0A BR9810402A (pt) 1997-06-03 1998-06-03 Engenheiramento de tioesterases de planta e divulgação de tioesterases de planta tendo especificidade de substrato

Country Status (9)

Country Link
US (1) US6150512A (pt)
EP (2) EP1003884B1 (pt)
JP (1) JP2002502263A (pt)
AT (1) ATE309372T1 (pt)
BR (1) BR9810402A (pt)
CA (1) CA2291452A1 (pt)
DE (1) DE69832283T2 (pt)
ES (1) ES2251770T3 (pt)
WO (1) WO1998055633A1 (pt)

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CA2382693C (en) * 1999-08-26 2013-10-01 Calgene Llc Nucleic acid sequences and methods of use for the production of plants with modified polyunsaturated fatty acids
US7531718B2 (en) * 1999-08-26 2009-05-12 Monsanto Technology, L.L.C. Nucleic acid sequences and methods of use for the production of plants with modified polyunsaturated fatty acids
US7166771B2 (en) * 2002-06-21 2007-01-23 Monsanto Technology Llc Coordinated decrease and increase of gene expression of more than one gene using transgenic constructs
US7566813B2 (en) * 2002-03-21 2009-07-28 Monsanto Technology, L.L.C. Nucleic acid constructs and methods for producing altered seed oil compositions
AR039113A1 (es) * 2002-03-21 2005-02-09 Monsanto Technology Llc Construcciones de acidos nucleicos y metodos para producir composiciones de aceites de semillas alteradas
AR039717A1 (es) * 2002-06-21 2005-03-09 Monsanto Technology Llc Construcciones de arn de intrones cadena doble y usos de las mismas
JP4840360B2 (ja) * 2005-06-30 2011-12-21 トヨタ自動車株式会社 ペトロセリン酸生合成に関与する新規遺伝子、ペトロセリン酸の製造方法
CN101421406B (zh) 2006-02-13 2016-08-31 孟山都技术有限公司 用于产生改变的种子油组成的核酸构建体和方法
RU2483057C2 (ru) 2006-06-28 2013-05-27 Ньюселис Инк. Смеси жирных кислот и их применение
CA2662607A1 (en) * 2006-09-08 2008-03-13 Kaneka Corporation Composition comprising reduced coenzyme q10 and lysolecithin
MX2010006539A (es) * 2007-12-11 2011-02-23 Synthetic Genomics Inc Secrecion de acidos grasos por parte de microorganismos fotosinteticos.
CA2710767C (en) * 2007-12-27 2016-09-27 Cibus Llc Alkylester fatty acid blends and uses therefor
WO2010021711A1 (en) * 2008-08-18 2010-02-25 Ls9, Inc. Systems and methods for the production of mixed fatty esters
JP5767585B2 (ja) 2008-09-29 2015-08-19 モンサント テクノロジー エルエルシー 大豆遺伝子組換え事象ｍｏｎ８７７０５およびその検出方法
EP2448955B1 (en) 2009-06-29 2015-09-30 Synthetic Genomics, Inc. Acyl-acp thioesterase genes and uses therefor
CA2782423C (en) * 2009-12-18 2019-06-18 Cargill Incorporated Brassica plants yielding oils with a low total saturated fatty acid content
EP2576765A4 (en) 2010-05-25 2013-12-18 Cargill Inc KOHLPFLANZEN FOR OBTAINING OILS WITH LOW CONTENT ON ALPHA-LINOLENIC ACID
US9695434B2 (en) 2010-05-25 2017-07-04 Cargill, Incorporated Brassica plants yielding oils with a low alpha linolenic acid content
US9399768B2 (en) 2011-07-27 2016-07-26 Iowa State University Research Foundation, Inc. Materials and methods for using an acyl-acyl carrier protein thioesterase and mutants and chimeras thereof in fatty acid synthesis
US8951762B2 (en) 2011-07-27 2015-02-10 Iowa State University Research Foundation, Inc. Materials and methods for using an acyl—acyl carrier protein thioesterase and mutants and chimeras thereof in fatty acid synthesis
WO2013096082A1 (en) 2011-12-20 2013-06-27 Codexis, Inc. Fatty alcohol forming acyl reductase (far) variants and methods of use
WO2013159055A1 (en) 2012-04-20 2013-10-24 Codexis, Inc. Production of fatty alcohols from engineered microorganisms
JP6006553B2 (ja) * 2012-07-10 2016-10-12 花王株式会社 組換えクラミドモナス属及びそれを用いたラウリン酸高含有油脂の製造方法
WO2014015278A1 (en) 2012-07-20 2014-01-23 Codexis, Inc. Production of fatty alcohols from engineered microorganisms
JP5925651B2 (ja) * 2012-09-20 2016-05-25 花王株式会社 チオエステラーゼ改変体を用いた脂質の製造方法
CA2888197C (en) 2012-10-15 2023-03-07 Genomatica, Inc. Microorganisms and methods for production of specific length fatty alcohols and related compounds
US9512447B2 (en) 2012-12-14 2016-12-06 Codexis, Inc. Modified native beta-ketoacyl-ACP synthases and engineered microorganisms
JP6491881B2 (ja) 2012-12-27 2019-03-27 花王株式会社 アシル−ａｃｐチオエステラーゼ
US9567615B2 (en) * 2013-01-29 2017-02-14 Terravia Holdings, Inc. Variant thioesterases and methods of use
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CA2206984A1 (en) * 1995-05-15 1996-11-21 Calgene, Inc. Engineering plant thioesterases and disclosure of plant thioesterases having novel substrate specificity
CA2205657A1 (en) * 1995-09-29 1997-04-03 Calgene, Inc. Plant stearoyl-acp thioesterase sequences and methods to increase stearate content in plant seed oils

Also Published As

Publication number Publication date
DE69832283T2 (de) 2006-08-10
JP2002502263A (ja) 2002-01-22
CA2291452A1 (en) 1998-12-10
US6150512A (en) 2000-11-21
ATE309372T1 (de) 2005-11-15
EP1003884A1 (en) 2000-05-31
EP1003884B1 (en) 2005-11-09
WO1998055633A1 (en) 1998-12-10
EP1605048A1 (en) 2005-12-14
DE69832283D1 (de) 2005-12-15
ES2251770T3 (es) 2006-05-01

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Legal Events

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B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: DE ACORDO COM O ART. 37, OPINO PELO INDEFERIMENTO DO PRESENTE PEDIDO, UMA VEZ QUE NAO ATENDE AOS REQUISITOS DE NOVIDADE ( ART. 8O COMBINADO COM O ART. 11 DA LPI 9279/96) E DE ATIVIDADE INVENTIVA ( ART. 8O COMBINADO COM O ART. 13 DA LPI 9279/96).

B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: MANTIDO O INDEFERIMENTO UMA VEZ QUE NAO FOI APRESENTADO RECURSO DENTRO DO PRAZO LEGAL.