AR039113A1 - Construcciones de acidos nucleicos y metodos para producir composiciones de aceites de semillas alteradas - Google Patents

Construcciones de acidos nucleicos y metodos para producir composiciones de aceites de semillas alteradas

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Publication number
AR039113A1
AR039113A1 ARP030101010A ARP030101010A AR039113A1 AR 039113 A1 AR039113 A1 AR 039113A1 AR P030101010 A ARP030101010 A AR P030101010A AR P030101010 A ARP030101010 A AR P030101010A AR 039113 A1 AR039113 A1 AR 039113A1
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AR
Argentina
Prior art keywords
gene
weight
dna sequences
ketoacyl
expressed
Prior art date
Application number
ARP030101010A
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English (en)
Inventor
Joanne J Fillatti
Neal A Bringe
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Monsanto Technology Llc
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Publication date
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Publication of AR039113A1 publication Critical patent/AR039113A1/es

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    • CCHEMISTRY; METALLURGY
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    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23DEDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
    • A23D9/00Other edible oils or fats, e.g. shortenings, cooking oils
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/142Amino acids; Derivatives thereof
    • A23K20/147Polymeric derivatives, e.g. peptides or proteins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/158Fatty acids; Fats; Products containing oils or fats
    • CCHEMISTRY; METALLURGY
    • C10PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
    • C10LFUELS NOT OTHERWISE PROVIDED FOR; NATURAL GAS; SYNTHETIC NATURAL GAS OBTAINED BY PROCESSES NOT COVERED BY SUBCLASSES C10G, C10K; LIQUEFIED PETROLEUM GAS; ADDING MATERIALS TO FUELS OR FIRES TO REDUCE SMOKE OR UNDESIRABLE DEPOSITS OR TO FACILITATE SOOT REMOVAL; FIRELIGHTERS
    • C10L1/00Liquid carbonaceous fuels
    • C10L1/02Liquid carbonaceous fuels essentially based on components consisting of carbon, hydrogen, and oxygen only
    • C10L1/026Liquid carbonaceous fuels essentially based on components consisting of carbon, hydrogen, and oxygen only for compression ignition
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11BPRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00Production of fats or fatty oils from raw materials
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237Externally regulated expression systems
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0071Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083Miscellaneous (1.14.99)
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Animal Husbandry (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Nutrition Science (AREA)
  • Mycology (AREA)
  • Physiology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Reivindicación 1: Una semilla de soja, caracterizada porque presenta una composición de aceites que comprende entre 55 y 75% en peso de ácido oleico, entre 10 y 30% en peso de ácido linoleico, 4% o menos en peso de ácido linolénico y 6% o menos en peso de ácidos grasos saturados. Reivindicación 2: La semilla de soja de la cláusula 1, caracterizada porque dicha semilla comprende una molécula de ácido nucleico recombinante, donde dicha molécula comprende un primer conjunto de secuencias de ADN que, cuando se expresa en una célula huésped, tiene la capacidad de suprimir la expresión endógena de al menos dos genes seleccionados del grupo formado por los genes FAD2, FAD3 y FATB y un segundo conjunto de secuencias de ADN que, cuando se expresa en una célula huésped, tiene la capacidad de incrementar la expresión endógena de al menos un gen seleccionado del grupo formado por un gen de b-cetoacil-ACP sintetasa I, un gen de b-cetoacil-ACP sintetasa IV y un gen de d-9-desaturasa. Reivindicación 8: Aceite derivado de la semilla de soja de la cláusula 2, caracterizado porque dicho aceite presenta un contenido reducido de ácidos grasos saturados, un contenido reducido de ácidos grasos poliinsaturados y un mayor contenido de ácido oleico con relación a un aceite derivado de las semillas de una planta de antecedentes genéticos similares pero que no contiene la molécula de ácido nucleico recombinante. Reivindicación 11: Una semilla de soja caracterizada porque presenta una composición de aceites que comprende entre 69 y 73% en peso de ácido oleico, entre 21 y 24% en peso de ácido linoleico, entre 0,5 y 3% en peso de ácido linolénico y 2-3% en peso de ácidos grasos saturados. Reivindicación 12: Un aceite de soja crudo, caracterizado porque presenta una composición de aceites que comprende entre 55 y 75% en peso de ácido oleico, entre 10 y 30% en peso de ácido linoleico, 4% o menos en peso de ácido linolénico y 6% o menos en peso de ácidos grasos saturados. Reivindicación 17: Una planta de soja transformada portadora de semillas, caracterizada porque dichas semillas presentan una composición de aceites que comprende entre 55 y 75% en peso de ácido oleico, entre 10 y 30% en peso de ácido linoleico, 4% o menos en peso de ácido linolénico y 6% o menos en peso de ácidos grasos saturados. Reivindicación 18: La planta de soja transformada de la cláusula 17, caracterizada porque dicha planta de soja transformada comprende una molécula de ácido nucleico recombinante que comprende: un primer conjunto de secuencias de ADN que, cuando se expresa en una célula huésped, tiene la capacidad de suprimir la expresión endógena de un gen de FAD2 y un gen de FAD3 y un segundo conjunto de secuencias de ADN que, cuando se expresa en una célula huésped, tiene la capacidad de incrementar la expresión endógena de al menos un gen seleccionado del grupo formado por un gen de b-cetoacil-ACP sintetasa I, un gen de b-cetoacil-ACP sintetasa IV y un gen de d-9-desaturasa. Reivindicación 27: Un método para alterar la composición de aceites de una célula vegetal, caracterizado porque comprende: (A) transformar una célula vegetal con una molécula de ácido nucleico recombinante que comprende un primer conjunto de secuencias de ADN que, cuando se expresa en una célula huésped, tiene la capacidad de suprimir la expresión endógena de al menos dos genes seleccionados del grupo formado por los genes FAD2, FAD3 y FATB y un segundo conjunto de secuencias de ADN que, cuando se expresa en una célula huésped, tiene la capacidad de incrementar la expresión endógena de al menos un gen seleccionado del grupo formado por un gen de b-cetoacil-ACP sintetasa I, un gen de b-cetoacil-ACP sintetasa IV y un gen de d-9-desaturasa; y (B) cultivar dicha célula vegetal bajo condiciones que permitan la transcripción de dicho primer conjunto de secuencias de ADN y dicho segundo conjunto de secuencias de ADN, con lo cual se altera la composición de aceites con relación a una célula vegetal de antecedentes genéticos similares pero que no contiene la molécula de ácido nucleico recombinante. Reivindicación 36: Una molécula de ácido nucleico recombinante, caracterizada porque comprende: un primer conjunto de secuencias de ADN que, cuando se expresa en una célula huésped, tiene la capacidad de suprimir la expresión endógena de al menos dos genes seleccionados del grupo formado por los genes FAD2, FAD3 y FATB; y un segundo conjunto de secuencias de ADN que, cuando se expresa en una célula huésped, tiene la capacidad de incrementar la expresión endógena de al menos un gen seleccionado del grupo formado por un gen de b-cetoacil-ACP sintetasa I, un gen de b-cetoacil-ACP sintetasa IV y un gen de d-9-desaturasa.
ARP030101010A 2002-03-21 2003-03-21 Construcciones de acidos nucleicos y metodos para producir composiciones de aceites de semillas alteradas AR039113A1 (es)

Applications Claiming Priority (2)

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US36579402P 2002-03-21 2002-03-21
US39018502P 2002-06-21 2002-06-21

Publications (1)

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AR039113A1 true AR039113A1 (es) 2005-02-09

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US (3) US20060080750A1 (es)
EP (2) EP1484959A4 (es)
CN (1) CN100510084C (es)
AR (1) AR039113A1 (es)
AU (1) AU2003214247B2 (es)
BR (1) BR0308614A (es)
CA (1) CA2479587A1 (es)
MX (1) MXPA04009134A (es)
WO (1) WO2003080802A2 (es)
ZA (1) ZA200407546B (es)

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US20060080750A1 (en) 2006-04-13
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