BR9809240A - Clone de hpiv recombinante, processos para preparar vìrus parainfluenza humano recombinante e para introduzir uma mutação especìfica de sìtio dentro do genoma de um vìrus parainfluenza humano, e, célula hospedeira - Google Patents
Clone de hpiv recombinante, processos para preparar vìrus parainfluenza humano recombinante e para introduzir uma mutação especìfica de sìtio dentro do genoma de um vìrus parainfluenza humano, e, célula hospedeiraInfo
- Publication number
- BR9809240A BR9809240A BR9809240-5A BR9809240A BR9809240A BR 9809240 A BR9809240 A BR 9809240A BR 9809240 A BR9809240 A BR 9809240A BR 9809240 A BR9809240 A BR 9809240A
- Authority
- BR
- Brazil
- Prior art keywords
- hpiv
- clone
- human parainfluenza
- parainfluenza virus
- genome
- Prior art date
Links
- 241000700605 Viruses Species 0.000 title abstract 7
- 208000002606 Paramyxoviridae Infections Diseases 0.000 title abstract 6
- 238000000034 method Methods 0.000 title abstract 4
- 230000035772 mutation Effects 0.000 title abstract 3
- 239000002773 nucleotide Substances 0.000 abstract 5
- 125000003729 nucleotide group Chemical group 0.000 abstract 5
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- 102000004169 proteins and genes Human genes 0.000 abstract 3
- 108091026890 Coding region Proteins 0.000 abstract 2
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 abstract 2
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 abstract 2
- 101001065501 Escherichia phage MS2 Lysis protein Proteins 0.000 abstract 2
- 210000004027 cell Anatomy 0.000 abstract 2
- 102000053642 Catalytic RNA Human genes 0.000 abstract 1
- 108090000994 Catalytic RNA Proteins 0.000 abstract 1
- 101900161954 Human parainfluenza 3 virus Phosphoprotein Proteins 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 102100034574 P protein Human genes 0.000 abstract 1
- 101710181008 P protein Proteins 0.000 abstract 1
- 101710177166 Phosphoprotein Proteins 0.000 abstract 1
- 239000000427 antigen Substances 0.000 abstract 1
- 102000036639 antigens Human genes 0.000 abstract 1
- 108091007433 antigens Proteins 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 210000005260 human cell Anatomy 0.000 abstract 1
- 208000015181 infectious disease Diseases 0.000 abstract 1
- 230000002458 infectious effect Effects 0.000 abstract 1
- 108091092562 ribozyme Proteins 0.000 abstract 1
- 238000003786 synthesis reaction Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/18011—Paramyxoviridae
- C12N2760/18611—Respirovirus, e.g. Bovine, human parainfluenza 1,3
- C12N2760/18621—Viruses as such, e.g. new isolates, mutants or their genomic sequences
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/18011—Paramyxoviridae
- C12N2760/18611—Respirovirus, e.g. Bovine, human parainfluenza 1,3
- C12N2760/18622—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/18011—Paramyxoviridae
- C12N2760/18611—Respirovirus, e.g. Bovine, human parainfluenza 1,3
- C12N2760/18634—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/18011—Paramyxoviridae
- C12N2760/18611—Respirovirus, e.g. Bovine, human parainfluenza 1,3
- C12N2760/18651—Methods of production or purification of viral material
- C12N2760/18652—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
''CLONE DE HPIV RECOMBINANTE, PROCESSOS PARA PREPARAR VìRUS PARAINFLUENZA HUMANO RECOMBINANTE E PARA INTRODUZIR UMA MUTAçãO ESPECìFICA DE SìTIO DENTRO DO GENOMA DE UM VìRUS PARAINFLUENZA HUMANO, E, CéLULA HOSPEDEIRA''. é fornecido um sistema para gerar vírus parainfluenza humano recombinante, particularmente vírus parainfluenza humano, recombinante, infeccioso, tipo 3 (HPIV-3). Em uma versão, o sistema compreende um clone compreendendo uma seq³ência de nucleotídeo que codifica um anti-genoma de comprimento total, de sentido positivo, do HPIV e pelo menos um clone de suporte compreendendo uma seq³ência de nucleotídeo que codifica a proteína P do HPIV e a proteína L do HPIV. Em outra versão, o sistema compreende ainda um clone de suporte, que compreende uma seq³ência de nucleotídeo que codifica a proteína NP do HPIV. A presente invenção também fornece um clone que compreende uma seq³ência de nucleotídeo codificando o antigenoma de comprimento total, de sentido positivo do HPIV-3. O clone também compreende um promotor de polimerase do RNA, operativamente ligado à seq³ência de codificação do antigenoma do HPIV-3. Em uma versão preferida, o clone compreende também um promotor da polimerase do RNA, operativamente ligado à seq³ência codificadora do antigenoma do HPIV-3. Em uma versão preferida, o clone compreende ainda uma seq³ência de nucleotídeo que codifica uma ribozima imediatamente a jusante da seq³ência codificando o anti-genoma do HPIV-3. A presente invenção também refere-se a um processo de preparar vírus HPIV-3 recombinante, tendo mutações específicas de sítio no genoma HPIV-3. O processo compreende preparar um clone compreendendo uma seq³ência codificando o antigenoma do HPIV-3 modificado; introduzir o clone do HPIV-3 modificado e suportar clones que compreendem seq³ências de nucleotídeo codificando uma proteína P de HPIV-3, uma proteína L do HPIV-3 e, preferivelmente, uma proteína NP do HPIV-3 dentro das células hospedeiras; e cultivar as células hospedeiras sob condições que permitam a síntese do antigenoma do HPIV-3 modificado e das proteínas L, P e NP do HPIV-3.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US4580597P | 1997-05-07 | 1997-05-07 | |
| PCT/US1998/009270 WO1998050405A1 (en) | 1997-05-07 | 1998-05-06 | An infectious clone for human parainfluenza virus type 3 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR9809240A true BR9809240A (pt) | 2000-06-27 |
Family
ID=21939986
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR9809240-5A BR9809240A (pt) | 1997-05-07 | 1998-05-06 | Clone de hpiv recombinante, processos para preparar vìrus parainfluenza humano recombinante e para introduzir uma mutação especìfica de sìtio dentro do genoma de um vìrus parainfluenza humano, e, célula hospedeira |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US6248578B1 (pt) |
| EP (1) | EP0981537B1 (pt) |
| JP (1) | JP2002512519A (pt) |
| KR (2) | KR100585946B1 (pt) |
| CN (1) | CN1208340C (pt) |
| AT (1) | ATE367397T1 (pt) |
| AU (1) | AU736586B2 (pt) |
| BR (1) | BR9809240A (pt) |
| CA (1) | CA2289776C (pt) |
| CY (1) | CY1107730T1 (pt) |
| DE (1) | DE69838097T2 (pt) |
| DK (1) | DK0981537T3 (pt) |
| ES (1) | ES2289782T3 (pt) |
| HK (1) | HK1025973A1 (pt) |
| PT (1) | PT981537E (pt) |
| WO (1) | WO1998050405A1 (pt) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7465574B2 (en) | 1994-09-30 | 2008-12-16 | Medimmune, Llc | Recombinant RSV virus expression systems and vaccines |
| US6830748B1 (en) | 1997-09-26 | 2004-12-14 | Medimmune Vaccines, Inc. | Recombinant RSV virus expression systems and vaccines |
| US6764685B1 (en) * | 2000-03-21 | 2004-07-20 | Medimmune Vaccines, Inc. | Recombinant parainfluenza virus expression systems and vaccines |
| CN1646684B (zh) * | 2002-02-21 | 2010-10-06 | 免疫医疗疫苗公司 | 重组副流感病毒表达系统以及包含源自间质肺病毒的异种抗原的疫苗 |
| US20100316991A1 (en) * | 2009-06-11 | 2010-12-16 | Utah State University | Human parainfluenza virus type 3 expressing the enhanced green fluorescent protein for use in high-throughput antiviral assays |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5166057A (en) | 1989-08-28 | 1992-11-24 | The Mount Sinai School Of Medicine Of The City University Of New York | Recombinant negative strand rna virus expression-systems |
| ES2210273T5 (es) * | 1994-07-18 | 2010-03-29 | Conzelmann, Karl-Klaus, Prof. Dr. | Virus con arn de cadena negativa no segmentado recombinante infeccioso. |
| US5869036A (en) * | 1995-12-08 | 1999-02-09 | St. Louis University | Live attenuated vaccines based on CP45 HPIV-3 strain and method to ensure attenuation in such vaccine |
-
1998
- 1998-05-06 BR BR9809240-5A patent/BR9809240A/pt not_active Application Discontinuation
- 1998-05-06 KR KR1019997010246A patent/KR100585946B1/ko not_active IP Right Cessation
- 1998-05-06 AT AT98922118T patent/ATE367397T1/de not_active IP Right Cessation
- 1998-05-06 KR KR1020067002175A patent/KR100678818B1/ko not_active IP Right Cessation
- 1998-05-06 CN CNB988048833A patent/CN1208340C/zh not_active Expired - Fee Related
- 1998-05-06 PT PT98922118T patent/PT981537E/pt unknown
- 1998-05-06 AU AU74731/98A patent/AU736586B2/en not_active Ceased
- 1998-05-06 WO PCT/US1998/009270 patent/WO1998050405A1/en active IP Right Grant
- 1998-05-06 ES ES98922118T patent/ES2289782T3/es not_active Expired - Lifetime
- 1998-05-06 US US09/073,492 patent/US6248578B1/en not_active Expired - Fee Related
- 1998-05-06 DE DE69838097T patent/DE69838097T2/de not_active Expired - Fee Related
- 1998-05-06 CA CA002289776A patent/CA2289776C/en not_active Expired - Fee Related
- 1998-05-06 JP JP54846598A patent/JP2002512519A/ja active Pending
- 1998-05-06 EP EP98922118A patent/EP0981537B1/en not_active Expired - Lifetime
- 1998-05-06 DK DK98922118T patent/DK0981537T3/da active
-
2000
- 2000-08-21 HK HK00105223A patent/HK1025973A1/xx not_active IP Right Cessation
-
2007
- 2007-09-10 CY CY20071101163T patent/CY1107730T1/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| KR100678818B1 (ko) | 2007-02-07 |
| KR20010012294A (ko) | 2001-02-15 |
| WO1998050405A1 (en) | 1998-11-12 |
| DE69838097D1 (de) | 2007-08-30 |
| JP2002512519A (ja) | 2002-04-23 |
| KR20060014461A (ko) | 2006-02-15 |
| EP0981537A1 (en) | 2000-03-01 |
| CN1208340C (zh) | 2005-06-29 |
| ES2289782T3 (es) | 2008-02-01 |
| HK1025973A1 (en) | 2000-12-01 |
| CY1107730T1 (el) | 2013-04-18 |
| ATE367397T1 (de) | 2007-08-15 |
| EP0981537B1 (en) | 2007-07-18 |
| KR100585946B1 (ko) | 2006-06-07 |
| DK0981537T3 (da) | 2007-11-19 |
| US6248578B1 (en) | 2001-06-19 |
| DE69838097T2 (de) | 2008-04-10 |
| AU736586B2 (en) | 2001-08-02 |
| CN1255140A (zh) | 2000-05-31 |
| PT981537E (pt) | 2007-10-16 |
| EP0981537A4 (en) | 2002-09-18 |
| CA2289776C (en) | 2008-10-21 |
| AU7473198A (en) | 1998-11-27 |
| CA2289776A1 (en) | 1998-11-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Van Der Werf et al. | Synthesis of infectious poliovirus RNA by purified T7 RNA polymerase. | |
| Jackson et al. | Product of per locus of Drosophila shares homology with proteoglycans | |
| DE69840361D1 (de) | Neue knochenmineralisierungsproteine, dna, vektoren, expressionssysteme | |
| BRPI9905902B8 (pt) | "molécula polinucleotídica isolada, plasmídeo, e método para produzir um vírus prrs norte-americano" | |
| Barrett et al. | Nucleotide sequence of the entire protein coding region of canine distemper virus polymerase-associated (P) protein mRNA | |
| Murphy et al. | A functional antigenomic promoter for the paramyxovirus simian virus 5 requires proper spacing between an essential internal segment and the 3′ terminus | |
| CA2236378A1 (en) | Recombinant sendai virus | |
| US20070202568A1 (en) | Insertion of Furin Protease Cleavage Sites in Membrane Proteins and Uses Thereof | |
| ES2075901T3 (es) | Sistemas de expresion de virus recombinantes de arn con cadena negativa y vacunas. | |
| BR9712592A (pt) | Processo para gerar um clone infeccioso, ácido nucléico recombinante, molécula do mesmo, vìrus de rna modificado, célula infectada com o mesmo, vacina, proteìna e/ou antìgeno, e, ensaio diagnóstico | |
| ATE253633T1 (de) | Cystic fibrosis gen | |
| PT95494A (pt) | Processo para a producao de dna recombinante compreendendo sequencias derivadas de virus de rna, e manipulacoes geneticas que o utilizam | |
| BR0016145A (pt) | Clones infecciosos | |
| BRPI0504945A (pt) | método para a produção de vìrus recombinante contendo sequências de nucleotìdeos codificantes do todo ou parte de proteìnas heterólogas, constructos de dna, vìrus recombinantes resultantes e composições vacinais contendo tais vìrus recombinantes para imunizar contra flavivirus e/ou outros patógenos | |
| ATE309367T1 (de) | Nukleotid- und aminosäuresequenzen der gene des hüllproteins 1 und des kernproteins von hepatitis c virus | |
| BR9803960A (pt) | Gene de desidrogenase de d-sorbitol. | |
| BR9809240A (pt) | Clone de hpiv recombinante, processos para preparar vìrus parainfluenza humano recombinante e para introduzir uma mutação especìfica de sìtio dentro do genoma de um vìrus parainfluenza humano, e, célula hospedeira | |
| EP0585549B2 (en) | Method for producing ectoprotein of hepatitis C virus | |
| CA2333313A1 (en) | Novel methods for rescue of rna viruses | |
| BR9815912A (pt) | Cepa viral de hepatite b induzida por vacina e seus usos | |
| Murakami et al. | High-level expression of exogenous genes by replication-competent retrovirus vectors with an internal ribosomal entry site | |
| Mosser et al. | Proteins of Rous-associated virus type 61: polypeptide stoichiometry and evidence that glycoprotein gp35 is not a cleavage product of gp85 | |
| Ghassemi et al. | Translational efficiency of BVDV IRES and EMCV IRES for T7 RNA polymerase driven cytoplasmic expression in mammalian cell lines | |
| Tsuchiya et al. | Ku antigen binds to Alu family DNA | |
| EP0457527A1 (en) | Plasmids, transformed animal cells and process for producing foreign protein |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
| B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
| B09B | Patent application refused [chapter 9.2 patent gazette] |
Free format text: INDEFIRO O PEDIDO DE ACORDO COM O(S) ARTIGO(S) 8O E 13 DA LPI. |
|
| B09B | Patent application refused [chapter 9.2 patent gazette] |
Free format text: MANTIDO O INDEFERIMENTO UMA VEZ QUE NAO FOI APRESENTADO RECURSO DENTRO DO PRAZO LEGAL. |
|
| B15K | Others concerning applications: alteration of classification |
Ipc: C07K 14/005 (2006.01), C12N 7/00 (2006.0 |