BR122020005905B1 - Método para produzir lmetionina - Google Patents

Método para produzir lmetionina Download PDF

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Publication number
BR122020005905B1
BR122020005905B1 BR122020005905-2A BR122020005905A BR122020005905B1 BR 122020005905 B1 BR122020005905 B1 BR 122020005905B1 BR 122020005905 A BR122020005905 A BR 122020005905A BR 122020005905 B1 BR122020005905 B1 BR 122020005905B1
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BR
Brazil
Prior art keywords
homoserine
activity
acetyl
microorganism
acetyl homoserine
Prior art date
Application number
BR122020005905-2A
Other languages
English (en)
Portuguese (pt)
Inventor
Hyun Ah Kim
Ju Hee Seo
Yong Uk Shin
So Young Kim
Sang Kyoum Kim
Kwang Ho Na
Jee Yeon Bae
Sung Kwang Son
Hye Ryun Yoo
Jin Geun Choi
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Cj Cheiljedang Corporation
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Publication date
Application filed by Cj Cheiljedang Corporation filed Critical Cj Cheiljedang Corporation
Publication of BR122020005905B1 publication Critical patent/BR122020005905B1/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01031—Homoserine O-acetyltransferase (2.3.1.31)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/12—Methionine; Cysteine; Cystine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/03—Acyl groups converted into alkyl on transfer (2.3.3)
    • C12Y203/03001—Citrate (Si)-synthase (2.3.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01039—Homoserine kinase (2.7.1.39)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01031—Phosphoenolpyruvate carboxylase (4.1.1.31)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
BR122020005905-2A 2014-06-23 2015-06-22 Método para produzir lmetionina BR122020005905B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR1020140076779A KR101641770B1 (ko) 2014-06-23 2014-06-23 O-아세틸 호모세린을 생산하는 미생물 및 상기 미생물을 이용하여 o-아세틸 호모세린을 생산하는 방법
KR10-2014-0076779 2014-06-23
PCT/KR2015/006307 WO2015199396A1 (ko) 2014-06-23 2015-06-22 O-아세틸 호모세린을 생산하는 미생물 및 상기 미생물을 이용하여 o-아세틸 호모세린을 생산하는 방법

Publications (1)

Publication Number Publication Date
BR122020005905B1 true BR122020005905B1 (pt) 2022-07-26

Family

ID=54938419

Family Applications (2)

Application Number Title Priority Date Filing Date
BR122020005905-2A BR122020005905B1 (pt) 2014-06-23 2015-06-22 Método para produzir lmetionina
BR112015029716-1A BR112015029716B1 (pt) 2014-06-23 2015-06-22 Microrganismo produtor de o-acetil homoserina e método de produção o-acetil homoserina usando o mesmo

Family Applications After (1)

Application Number Title Priority Date Filing Date
BR112015029716-1A BR112015029716B1 (pt) 2014-06-23 2015-06-22 Microrganismo produtor de o-acetil homoserina e método de produção o-acetil homoserina usando o mesmo

Country Status (14)

Country Link
US (2) US10465217B2 (https=)
EP (2) EP2998388B1 (https=)
JP (2) JP6336074B2 (https=)
KR (1) KR101641770B1 (https=)
CN (2) CN105392880B (https=)
AU (1) AU2015264911B2 (https=)
BR (2) BR122020005905B1 (https=)
DK (1) DK2998388T3 (https=)
ES (1) ES2833432T3 (https=)
MY (1) MY185764A (https=)
PL (1) PL2998388T3 (https=)
RU (1) RU2614253C1 (https=)
SG (1) SG11201509451WA (https=)
WO (1) WO2015199396A1 (https=)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111315877B (zh) * 2017-06-30 2023-08-08 Cj第一制糖株式会社 新型o-琥珀酰高丝氨酸转移酶变体及使用其生产o-琥珀酰高丝氨酸的方法
CN110129294B (zh) * 2018-02-02 2021-12-24 中国科学院天津工业生物技术研究所 高丝氨酸乙酰基转移酶突变体及其宿主细胞和应用
KR101915433B1 (ko) 2018-02-13 2018-11-05 씨제이제일제당 (주) 시트레이트 신타아제 (Citrate synthase)의 활성이 약화된 변이형 폴리펩타이드 및 이를 이용한 L-아미노산 생산방법
CN113122486B (zh) * 2019-12-31 2023-06-13 江南大学 一种丙二酸的全生物合成的方法
KR102285951B1 (ko) 2020-01-30 2021-08-04 씨제이제일제당 주식회사 시트레이트 신타아제의 활성이 약화된 신규한 변이형 폴리펩티드 및 이를 이용한 l-아미노산 생산 방법
CN115427580A (zh) * 2020-02-07 2022-12-02 代谢探索者公司 用于改善依克多因产生的经修饰的微生物和方法
KR102344689B1 (ko) * 2020-09-01 2021-12-29 씨제이제일제당 주식회사 L-발린 생산 미생물 및 이를 이용한 l-발린 생산 방법
CN112501144B (zh) * 2021-02-02 2021-04-20 中国科学院天津工业生物技术研究所 高丝氨酸乙酰转移酶突变体及其在生产o-乙酰高丝氨酸中的应用
KR102525073B1 (ko) * 2021-03-10 2023-04-24 씨제이제일제당 주식회사 신규한 시트레이트 신타아제 변이체 및 이를 이용한 l-발린 생산 방법
KR102525074B1 (ko) * 2021-03-10 2023-04-24 씨제이제일제당 주식회사 신규한 시트레이트 신타아제 변이체 및 이를 이용한 o-아세틸-l-호모세린 또는 l-메티오닌 생산 방법
KR102525072B1 (ko) * 2021-03-10 2023-04-24 씨제이제일제당 주식회사 신규한 시트레이트 신타아제 변이체 및 이를 이용한 l-아미노산 생산 방법
CN113388564B (zh) * 2021-06-04 2022-03-01 浙江工业大学 一种o-乙酰-l-高丝氨酸生产菌、构建方法及应用
KR102952497B1 (ko) * 2022-03-28 2026-04-15 씨제이제일제당 주식회사 O-아세틸 호모세린 생산 미생물 및 이를 이용한 o-아세틸 호모세린 또는 l-메치오닌 생산 방법
KR20230139598A (ko) * 2022-03-28 2023-10-05 씨제이제일제당 (주) O-아세틸 호모세린 생산 미생물 및 이를 이용한 o-아세틸 호모세린 또는 l-메치오닌 생산 방법
KR20240147322A (ko) 2023-03-31 2024-10-08 씨제이제일제당 (주) 미생물 발효를 활용한 o-아세틸 호모세린 제조 공정

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58170488A (ja) 1982-03-31 1983-10-07 Ajinomoto Co Inc 発酵法によるｌ−アスパラギン酸の製造法
WO2005054490A1 (en) * 2003-12-05 2005-06-16 Ajinomoto Co., Inc. L-threonine producing bacterium belonging to the genus escherichia and method for producing l-threonine
JP4913818B2 (ja) * 2005-10-31 2012-04-11 エボニック デグサ ゲーエムベーハー Ｌ−メチオニン調製のための微生物および方法
WO2008013996A2 (en) * 2006-07-27 2008-01-31 Gevo Inc. Engineered microorganisms for increasing product yield in biotransformations, related methods and systems
KR100905381B1 (ko) 2006-07-28 2009-06-30 씨제이제일제당 (주) Ｌ-메치오닌 전구체 생산 균주 및 상기 ｌ-메치오닌전구체로부터의 ｌ-메치오닌 및 유기산의 생산방법
JP5656183B2 (ja) * 2006-08-22 2015-01-21 国立大学法人 東京医科歯科大学 滑膜由来間葉幹細胞（ＭＳＣｓ）の軟骨・半月板再生への応用
US7851180B2 (en) * 2008-04-04 2010-12-14 Cj Cheiljedang Corporation Microorganism producing L-methionine precursor and the method of producing L-methionine precursor using the microorganism
US9005952B2 (en) 2008-04-04 2015-04-14 Cj Cheiljedang Corporation Microorganism producing L-methionine precursor and the method of producing L-methionine precursor using the microorganism
RU2411289C2 (ru) * 2008-09-30 2011-02-10 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) Бактерия, принадлежащая к роду pantoea, - продуцент l-аспартата или метаболита, являющегося производным l-аспартата, и способ получения l-аспартата или метаболита, являющегося производным l-аспартата
US8609396B2 (en) * 2009-08-28 2013-12-17 Cj Cheiljedang Corporation Microorganism producing O-acetyl-homoserine and the method of producing O-acetyl-homoserine using the microorganism
US8283152B2 (en) * 2009-08-28 2012-10-09 Cj Cheiljedang Corporation Microorganism producing O-acetyl-homoserine and the method of producing O-acetyl-homoserine using the microorganism
WO2011073738A1 (en) 2009-12-14 2011-06-23 Metabolic Explorer Use of inducible promoters in the production of methionine
RU2010101135A (ru) * 2010-01-15 2011-07-20 Закрытое акционерное общество "Научно-исследовательский институт "Аджиномото-Генетика" (ЗАО АГРИ) (RU) Бактерия семейства enterobacteriaceae - продуцент l-аспартата или метаболитов, производных l-аспартата, и способ получения l-аспартата или метаболитов, производных l-аспартата
JP5758499B2 (ja) 2010-12-21 2015-08-05 シージェイ チェイルジェダン コーポレイション ホモセリンアセチルトランスフェラーゼ活性を有する変異型ポリペプチド及びそれを発現する微生物

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Publication number Publication date
AU2015264911B2 (en) 2017-11-23
PL2998388T3 (pl) 2021-05-17
JP6607974B2 (ja) 2019-11-20
KR101641770B1 (ko) 2016-07-22
BR112015029716A2 (pt) 2017-09-26
EP3783096B1 (en) 2024-03-13
KR20160000098A (ko) 2016-01-04
RU2614253C1 (ru) 2017-03-24
EP2998388A4 (en) 2017-03-22
SG11201509451WA (en) 2016-01-28
CN105392880A (zh) 2016-03-09
EP2998388B1 (en) 2020-10-07
JP6336074B2 (ja) 2018-06-06
BR112015029716B1 (pt) 2022-07-19
JP2018046875A (ja) 2018-03-29
EP2998388A1 (en) 2016-03-23
US10815509B2 (en) 2020-10-27
CN105392880B (zh) 2019-11-08
DK2998388T3 (da) 2020-11-23
AU2015264911A1 (en) 2016-01-21
US20170101655A1 (en) 2017-04-13
ES2833432T3 (es) 2021-06-15
CN110592153A (zh) 2019-12-20
WO2015199396A1 (ko) 2015-12-30
US20200024626A1 (en) 2020-01-23
EP3783096A1 (en) 2021-02-24
JP2016526927A (ja) 2016-09-08
MY185764A (en) 2021-06-04
US10465217B2 (en) 2019-11-05

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