BR112021021828A2 - Método para sintetizar rebaudiosídeo i e mutante ugt76g1 - Google Patents
Método para sintetizar rebaudiosídeo i e mutante ugt76g1Info
- Publication number
- BR112021021828A2 BR112021021828A2 BR112021021828A BR112021021828A BR112021021828A2 BR 112021021828 A2 BR112021021828 A2 BR 112021021828A2 BR 112021021828 A BR112021021828 A BR 112021021828A BR 112021021828 A BR112021021828 A BR 112021021828A BR 112021021828 A2 BR112021021828 A2 BR 112021021828A2
- Authority
- BR
- Brazil
- Prior art keywords
- rebaudioside
- synthesize
- ugt76g1 mutant
- ugt76g1
- mutant
- Prior art date
Links
- 229930188195 rebaudioside Natural products 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 239000001512 FEMA 4601 Substances 0.000 abstract 1
- HELXLJCILKEWJH-SEAGSNCFSA-N Rebaudioside A Natural products O=C(O[C@H]1[C@@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1)[C@@]1(C)[C@@H]2[C@](C)([C@H]3[C@@]4(CC(=C)[C@@](O[C@H]5[C@H](O[C@H]6[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O6)[C@@H](O[C@H]6[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O6)[C@H](O)[C@@H](CO)O5)(C4)CC3)CC2)CCC1 HELXLJCILKEWJH-SEAGSNCFSA-N 0.000 abstract 1
- 239000004383 Steviol glycoside Substances 0.000 abstract 1
- HSCJRCZFDFQWRP-JZMIEXBBSA-N UDP-alpha-D-glucose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OP(O)(=O)OP(O)(=O)OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C(NC(=O)C=C2)=O)O1 HSCJRCZFDFQWRP-JZMIEXBBSA-N 0.000 abstract 1
- HELXLJCILKEWJH-UHFFFAOYSA-N entered according to Sigma 01432 Natural products C1CC2C3(C)CCCC(C)(C(=O)OC4C(C(O)C(O)C(CO)O4)O)C3CCC2(C2)CC(=C)C21OC(C1OC2C(C(O)C(O)C(CO)O2)O)OC(CO)C(O)C1OC1OC(CO)C(O)C(O)C1O HELXLJCILKEWJH-UHFFFAOYSA-N 0.000 abstract 1
- 125000003147 glycosyl group Chemical group 0.000 abstract 1
- HELXLJCILKEWJH-NCGAPWICSA-N rebaudioside A Chemical compound O([C@H]1[C@H](O)[C@@H](CO)O[C@H]([C@@H]1O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O HELXLJCILKEWJH-NCGAPWICSA-N 0.000 abstract 1
- 235000019203 rebaudioside A Nutrition 0.000 abstract 1
- 229930182488 steviol glycoside Natural products 0.000 abstract 1
- 235000019411 steviol glycoside Nutrition 0.000 abstract 1
- 150000008144 steviol glycosides Chemical class 0.000 abstract 1
- 235000019202 steviosides Nutrition 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/44—Preparation of O-glycosides, e.g. glucosides
- C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/30—Artificial sweetening agents
- A23L27/33—Artificial sweetening agents containing sugars or derivatives
- A23L27/36—Terpene glycosides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01013—Sucrose synthase (2.4.1.13)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01017—Glucuronosyltransferase (2.4.1.17)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/99—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Nutrition Science (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Enzymes And Modification Thereof (AREA)
- Mycology (AREA)
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201862641590P | 2018-03-12 | 2018-03-12 | |
| US201862682260P | 2018-06-08 | 2018-06-08 | |
| US201862695252P | 2018-07-09 | 2018-07-09 | |
| US16/506,892 US11447808B2 (en) | 2018-03-12 | 2019-07-09 | Biosynthetic production of steviol glycoside rebaudioside I via variant enzymes |
| US16/679,032 US10883130B2 (en) | 2018-03-12 | 2019-11-08 | Biosynthetic production of steviol glycosides rebaudioside J and rebaudioside N |
| PCT/US2020/041394 WO2021007423A1 (en) | 2018-03-12 | 2020-07-09 | Biosynthetic production of steviol glycoside rebaudioside i via variant enzymes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112021021828A2 true BR112021021828A2 (pt) | 2022-01-04 |
Family
ID=67908465
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112021021828A BR112021021828A2 (pt) | 2018-03-12 | 2020-07-09 | Método para sintetizar rebaudiosídeo i e mutante ugt76g1 |
Country Status (8)
| Country | Link |
|---|---|
| US (4) | US11447808B2 (https=) |
| EP (2) | EP3765627A4 (https=) |
| JP (3) | JP7365707B2 (https=) |
| KR (1) | KR20210153670A (https=) |
| CN (1) | CN111868252B (https=) |
| BR (1) | BR112021021828A2 (https=) |
| MX (1) | MX2021013721A (https=) |
| WO (2) | WO2019178116A1 (https=) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111868252B (zh) | 2018-03-12 | 2025-06-03 | 科纳根公司 | 甜菊糖苷莱苞迪苷j和莱苞迪苷n的生物合成生产 |
| BR112021019470A2 (pt) * | 2019-03-29 | 2021-11-30 | Conagen Inc | Produção biossintética de udp-ramnose |
| CN112080480B (zh) * | 2019-06-14 | 2023-01-03 | 中国科学院分子植物科学卓越创新中心 | 糖基转移酶突变体及其应用 |
| WO2022253282A1 (zh) * | 2021-06-01 | 2022-12-08 | 弈柯莱生物科技(上海)股份有限公司 | 一种糖基转移酶及其应用 |
| US20250051818A1 (en) * | 2021-12-24 | 2025-02-13 | Samyang Corporation | Production of rebaudioside |
| CN119842657B (zh) * | 2025-03-18 | 2025-05-27 | 青岛奔月生物技术有限公司 | 蔗糖合酶突变体及其应用 |
Family Cites Families (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL128193A (en) * | 1999-01-22 | 2008-03-20 | Yeda Res & Dev | Rhamnosyl-transferase gene and uses thereof |
| US20060143729A1 (en) * | 2004-06-30 | 2006-06-29 | Ceres, Inc. | Nucleotide sequences and polypeptides encoded thereby useful for modifying plant characteristics |
| US8791253B2 (en) * | 2006-06-19 | 2014-07-29 | The Coca-Cola Company | Rebaudioside A composition and method for purifying rebaudioside A |
| BRPI1014253A2 (pt) * | 2009-07-02 | 2016-04-12 | Merz Pharma Gmbh & Co Kgaa | polinucleotídeo codificado um polipeptídeo de neurotoxina, vetor, célula hospedeira, anticorpo, uso de um polinucleotídeo, método para a fabricação de um polipeptídeo de neurotoxina e método para a fabricação de um medicamento |
| CA3128532A1 (en) * | 2011-08-08 | 2013-02-14 | Evolva Sa | Recombinant production of steviol glycosides |
| US9752174B2 (en) * | 2013-05-28 | 2017-09-05 | Purecircle Sdn Bhd | High-purity steviol glycosides |
| MX395285B (es) * | 2012-05-22 | 2025-03-25 | Purecircle Sdn Bhd | Glucosidos de esteviol de alta pureza. |
| MY199452A (en) * | 2013-02-06 | 2023-10-30 | Evolva Sa | Methods for improved production of rebaudioside d and rebaudioside m |
| US9522929B2 (en) | 2014-05-05 | 2016-12-20 | Conagen Inc. | Non-caloric sweetener |
| EP3683315A1 (en) | 2014-08-19 | 2020-07-22 | Purecircle SDN BHD | Method for preparing rebaudioside m |
| US11202461B2 (en) * | 2014-09-02 | 2021-12-21 | Purecircle Sdn Bhd | Stevia extracts |
| BR112017006163B1 (pt) * | 2014-10-03 | 2022-04-05 | Conagen Inc | Produto de consumo oral e método para adoçar o mesmo |
| CN107109453A (zh) | 2014-11-05 | 2017-08-29 | 马努斯生物合成股份有限公司 | 甜菊醇糖苷的微生物产生 |
| KR102569053B1 (ko) | 2015-04-14 | 2023-08-21 | 코나겐 인크. | 조작된 전체 세포 촉매를 사용한 비칼로리 감미료의 제조 |
| US10612065B2 (en) * | 2015-05-29 | 2020-04-07 | Cargill, Incorporated | Heat treatment to produce glycosides |
| EP3387136B1 (en) * | 2015-12-10 | 2022-01-05 | Evolva SA | Production of steviol glycosides in recombinant hosts |
| JP6771937B2 (ja) * | 2016-04-21 | 2020-10-21 | キヤノンメディカルシステムズ株式会社 | 医用画像表示装置および医用画像管理システム |
| PT3497222T (pt) * | 2016-08-12 | 2022-02-09 | Amyris Inc | Glicosiltransferase dependente de udp para produção de eficácia elevada de rebaudiosídeos |
| BR112019007504B1 (pt) * | 2016-10-14 | 2023-05-09 | Conagen Inc | Método de preparação de uma composição de glicosídeo de esteviol |
| CN116574775A (zh) | 2016-10-21 | 2023-08-11 | 百事可乐公司 | 一种酶法制备瑞鲍迪甙j的方法 |
| CN111868252B (zh) | 2018-03-12 | 2025-06-03 | 科纳根公司 | 甜菊糖苷莱苞迪苷j和莱苞迪苷n的生物合成生产 |
| MY204735A (en) * | 2018-07-16 | 2024-09-11 | Manus Bio Inc | Production of steviol glycosides through whole cell biotransformation |
| BR112021019470A2 (pt) * | 2019-03-29 | 2021-11-30 | Conagen Inc | Produção biossintética de udp-ramnose |
| WO2020264179A1 (en) * | 2019-06-25 | 2020-12-30 | Manus Bio, Inc. | Uridine diphosphate-dependent glycosyltransferase enzyme |
| US20240148037A1 (en) * | 2019-10-16 | 2024-05-09 | Sweegen, Inc. | Steviol glycoside formulations for food and beverages |
-
2019
- 2019-03-12 CN CN201980018867.0A patent/CN111868252B/zh active Active
- 2019-03-12 WO PCT/US2019/021876 patent/WO2019178116A1/en not_active Ceased
- 2019-03-12 JP JP2020546338A patent/JP7365707B2/ja active Active
- 2019-03-12 EP EP19767823.8A patent/EP3765627A4/en active Pending
- 2019-07-09 US US16/506,892 patent/US11447808B2/en active Active
- 2019-11-08 US US16/679,032 patent/US10883130B2/en active Active
-
2020
- 2020-07-09 WO PCT/US2020/041394 patent/WO2021007423A1/en not_active Ceased
- 2020-07-09 JP JP2021571495A patent/JP7305213B2/ja active Active
- 2020-07-09 EP EP20836091.7A patent/EP3997233A4/en active Pending
- 2020-07-09 BR BR112021021828A patent/BR112021021828A2/pt unknown
- 2020-07-09 KR KR1020217037306A patent/KR20210153670A/ko not_active Ceased
- 2020-07-09 MX MX2021013721A patent/MX2021013721A/es unknown
- 2020-12-02 US US17/109,473 patent/US11441165B2/en active Active
-
2022
- 2022-08-12 US US17/819,423 patent/US20220411843A1/en active Pending
-
2023
- 2023-07-04 JP JP2023110059A patent/JP2023126903A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US11441165B2 (en) | 2022-09-13 |
| CN111868252A (zh) | 2020-10-30 |
| MX2021013721A (es) | 2021-12-10 |
| JP2021515555A (ja) | 2021-06-24 |
| US20210189448A1 (en) | 2021-06-24 |
| JP7305213B2 (ja) | 2023-07-10 |
| JP2023126903A (ja) | 2023-09-12 |
| EP3997233A4 (en) | 2023-08-23 |
| US20200087695A1 (en) | 2020-03-19 |
| US11447808B2 (en) | 2022-09-20 |
| EP3765627A4 (en) | 2021-10-13 |
| US20200002742A1 (en) | 2020-01-02 |
| CN111868252B (zh) | 2025-06-03 |
| US20220411843A1 (en) | 2022-12-29 |
| WO2019178116A8 (en) | 2019-10-10 |
| EP3997233A1 (en) | 2022-05-18 |
| JP2022540306A (ja) | 2022-09-15 |
| WO2019178116A1 (en) | 2019-09-19 |
| US10883130B2 (en) | 2021-01-05 |
| KR20210153670A (ko) | 2021-12-17 |
| JP7365707B2 (ja) | 2023-10-20 |
| EP3765627A1 (en) | 2021-01-20 |
| WO2021007423A1 (en) | 2021-01-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| BR112021021828A2 (pt) | Método para sintetizar rebaudiosídeo i e mutante ugt76g1 | |
| NZ725866A (en) | A non-caloric sweetener | |
| MX2021005862A (es) | Glucosiltransferasas modificadas con ingenieria genetica y metodos de glucosilacion de glucosido de esteviol. | |
| MX2021001260A (es) | Glucosiltransferasas modificadas con ingenieria genetica y metodos de glucosilacion de glucosido de esteviol. | |
| BR112018074999A2 (pt) | glicosiltransferase dependente de udp para a produção de alta eficiência de rebaudiosídeo | |
| MX2018014533A (es) | Composiciones de glucósido de esteviol glucosilado y métodos para preperarlas. | |
| MY204735A (en) | Production of steviol glycosides through whole cell biotransformation | |
| MX2020001903A (es) | Novedoso polipeptido y procedimiento de produccion de imp mediante el uso del mismo. | |
| MY205372A (en) | Imp-producing microorganism and method of producing imp using the same | |
| PH12018502655A1 (en) | Enzymatic glycosylation of steviol glycosides and other compounds with glucose-1-phosphate | |
| BR112018012950A2 (pt) | produtos lácteos adoçados com glicosídeos de esteviol e enzima lactase | |
| BR112019007504A2 (pt) | produção biossintética de glicosídeos de steviol e seus processos | |
| MX349121B (es) | Produccion recombinante de esteviol glucosidos. | |
| BR112018002371A2 (pt) | método para a produção de glicosídeo(s) de esteviol | |
| BR112021015766A2 (pt) | Método de biossíntese da diosmina e/ou da hesperidina em um microrganismo | |
| MX2018012561A (es) | Produccion de glicosidos de esteviol en huespedes recombinantes. | |
| MX2024014263A (es) | Produccion biosintetica del rebaudiosido d4 de glucosido de esteviol a partir del rebaudiosido e | |
| EP4275516A3 (en) | Steviol glycoside compositions | |
| BR112021019470A2 (pt) | Produção biossintética de udp-ramnose | |
| PY21104309A (es) | Composición que comprende estevia glucosilada y que tiene una calidad de dulce mejorada | |
| MX2020003234A (es) | Metodos para preparar glucosidos de esteviol y usos de los mismos. | |
| PY2018536A (es) | Composición que comprende glucósido de esteviol transfructosilado | |
| MX2019004629A (es) | Metodo para preparar rebaudiosido c mediante el uso del metodo enzimatico. | |
| ZA202107583B (en) | Cytidine derivatives and methods of forming cytidine derivatives | |
| PY2015735A (es) | Composición que comprende esteviósido transglucosilado y rebaudiósido a transglucosilado |