CN111868252B - 甜菊糖苷莱苞迪苷j和莱苞迪苷n的生物合成生产 - Google Patents

甜菊糖苷莱苞迪苷j和莱苞迪苷n的生物合成生产 Download PDF

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CN111868252B
CN111868252B CN201980018867.0A CN201980018867A CN111868252B CN 111868252 B CN111868252 B CN 111868252B CN 201980018867 A CN201980018867 A CN 201980018867A CN 111868252 B CN111868252 B CN 111868252B
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seq
rebaudioside
reb
recombinant polypeptide
sequence
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CN111868252A (zh
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毛国红
M·巴腾
P·宏特
余晓丹
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Conagen Inc
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Conagen Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • A23L27/33—Artificial sweetening agents containing sugars or derivatives
    • A23L27/36—Terpene glycosides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01017—Glucuronosyltransferase (2.4.1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01013—Sucrose synthase (2.4.1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/99—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Nutrition Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Mycology (AREA)
CN201980018867.0A 2018-03-12 2019-03-12 甜菊糖苷莱苞迪苷j和莱苞迪苷n的生物合成生产 Active CN111868252B (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201862641590P 2018-03-12 2018-03-12
US62/641590 2018-03-12
US201862682260P 2018-06-08 2018-06-08
US62/682260 2018-06-08
US201862695252P 2018-07-09 2018-07-09
US62/695252 2018-07-09
PCT/US2019/021876 WO2019178116A1 (en) 2018-03-12 2019-03-12 Biosynthetic production of steviol glycosides rebaudioside j and rebaudioside n

Publications (2)

Publication Number Publication Date
CN111868252A CN111868252A (zh) 2020-10-30
CN111868252B true CN111868252B (zh) 2025-06-03

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Country Status (8)

Country Link
US (4) US11447808B2 (https=)
EP (2) EP3765627A4 (https=)
JP (3) JP7365707B2 (https=)
KR (1) KR20210153670A (https=)
CN (1) CN111868252B (https=)
BR (1) BR112021021828A2 (https=)
MX (1) MX2021013721A (https=)
WO (2) WO2019178116A1 (https=)

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* Cited by examiner, † Cited by third party
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CN111868252B (zh) 2018-03-12 2025-06-03 科纳根公司 甜菊糖苷莱苞迪苷j和莱苞迪苷n的生物合成生产
BR112021019470A2 (pt) * 2019-03-29 2021-11-30 Conagen Inc Produção biossintética de udp-ramnose
CN112080480B (zh) * 2019-06-14 2023-01-03 中国科学院分子植物科学卓越创新中心 糖基转移酶突变体及其应用
WO2022253282A1 (zh) * 2021-06-01 2022-12-08 弈柯莱生物科技(上海)股份有限公司 一种糖基转移酶及其应用
US20250051818A1 (en) * 2021-12-24 2025-02-13 Samyang Corporation Production of rebaudioside
CN119842657B (zh) * 2025-03-18 2025-05-27 青岛奔月生物技术有限公司 蔗糖合酶突变体及其应用

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WO2016073740A1 (en) * 2014-11-05 2016-05-12 Manus Biosynthesis, Inc. Microbial production of steviol glycosides
CN107105686A (zh) * 2014-09-02 2017-08-29 谱赛科美国股份有限公司 富含莱鲍迪苷d、e、n和/或o的甜菊提取物及其制备方法
WO2018031955A2 (en) * 2016-08-12 2018-02-15 Amyris, Inc. Udp-dependent glycosyltransferase for high efficiency production of rebaudiosides

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US8791253B2 (en) * 2006-06-19 2014-07-29 The Coca-Cola Company Rebaudioside A composition and method for purifying rebaudioside A
BRPI1014253A2 (pt) * 2009-07-02 2016-04-12 Merz Pharma Gmbh & Co Kgaa polinucleotídeo codificado um polipeptídeo de neurotoxina, vetor, célula hospedeira, anticorpo, uso de um polinucleotídeo, método para a fabricação de um polipeptídeo de neurotoxina e método para a fabricação de um medicamento
CA3128532A1 (en) * 2011-08-08 2013-02-14 Evolva Sa Recombinant production of steviol glycosides
US9752174B2 (en) * 2013-05-28 2017-09-05 Purecircle Sdn Bhd High-purity steviol glycosides
MX395285B (es) * 2012-05-22 2025-03-25 Purecircle Sdn Bhd Glucosidos de esteviol de alta pureza.
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US9522929B2 (en) 2014-05-05 2016-12-20 Conagen Inc. Non-caloric sweetener
EP3683315A1 (en) 2014-08-19 2020-07-22 Purecircle SDN BHD Method for preparing rebaudioside m
BR112017006163B1 (pt) * 2014-10-03 2022-04-05 Conagen Inc Produto de consumo oral e método para adoçar o mesmo
KR102569053B1 (ko) 2015-04-14 2023-08-21 코나겐 인크. 조작된 전체 세포 촉매를 사용한 비칼로리 감미료의 제조
US10612065B2 (en) * 2015-05-29 2020-04-07 Cargill, Incorporated Heat treatment to produce glycosides
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WO2018031955A2 (en) * 2016-08-12 2018-02-15 Amyris, Inc. Udp-dependent glycosyltransferase for high efficiency production of rebaudiosides

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US11441165B2 (en) 2022-09-13
CN111868252A (zh) 2020-10-30
MX2021013721A (es) 2021-12-10
JP2021515555A (ja) 2021-06-24
US20210189448A1 (en) 2021-06-24
JP7305213B2 (ja) 2023-07-10
JP2023126903A (ja) 2023-09-12
EP3997233A4 (en) 2023-08-23
US20200087695A1 (en) 2020-03-19
US11447808B2 (en) 2022-09-20
EP3765627A4 (en) 2021-10-13
US20200002742A1 (en) 2020-01-02
US20220411843A1 (en) 2022-12-29
WO2019178116A8 (en) 2019-10-10
EP3997233A1 (en) 2022-05-18
JP2022540306A (ja) 2022-09-15
WO2019178116A1 (en) 2019-09-19
US10883130B2 (en) 2021-01-05
BR112021021828A2 (pt) 2022-01-04
KR20210153670A (ko) 2021-12-17
JP7365707B2 (ja) 2023-10-20
EP3765627A1 (en) 2021-01-20
WO2021007423A1 (en) 2021-01-14

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