BR112019008385A2 - método de preparação de células de levedura eletrocompetentes e métodos de uso das referidas células - Google Patents

método de preparação de células de levedura eletrocompetentes e métodos de uso das referidas células

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Publication number
BR112019008385A2
BR112019008385A2 BR112019008385A BR112019008385A BR112019008385A2 BR 112019008385 A2 BR112019008385 A2 BR 112019008385A2 BR 112019008385 A BR112019008385 A BR 112019008385A BR 112019008385 A BR112019008385 A BR 112019008385A BR 112019008385 A2 BR112019008385 A2 BR 112019008385A2
Authority
BR
Brazil
Prior art keywords
cells
methods
electrocompetent yeast
yeast cells
preparing electrocompetent
Prior art date
Application number
BR112019008385A
Other languages
English (en)
Inventor
Maurer Dominik
Unverdorben Felix
Bunk Sebastian
Original Assignee
Immatics Biotechnologies Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Immatics Biotechnologies Gmbh filed Critical Immatics Biotechnologies Gmbh
Publication of BR112019008385A2 publication Critical patent/BR112019008385A2/pt

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503Immunoglobulin superfamily
    • C07K14/7051T-cell receptor (TcR)-CD3 complex
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1037Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • C12N15/902Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/905Stable introduction of foreign DNA into chromosome using homologous recombination in yeast

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

a presente invenção refere-se à transformação e melhoramento de células de levedura e a bibliotecas de células de levedura assim transformadas. mais especificamente, a presente invenção refere-se à transformação de leveduras por eletroporação.
BR112019008385A 2016-11-15 2017-11-13 método de preparação de células de levedura eletrocompetentes e métodos de uso das referidas células BR112019008385A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102016121899.5A DE102016121899A1 (de) 2016-11-15 2016-11-15 Verfahren zur Herstellung von elektrokompetenten Hefezellen und Verfahren zur Verwendung dieser Zellen
PCT/EP2017/079011 WO2018091396A1 (en) 2016-11-15 2017-11-13 Method for preparing electrocompetent yeast cells, and method for using said cells

Publications (1)

Publication Number Publication Date
BR112019008385A2 true BR112019008385A2 (pt) 2019-07-09

Family

ID=60484335

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112019008385A BR112019008385A2 (pt) 2016-11-15 2017-11-13 método de preparação de células de levedura eletrocompetentes e métodos de uso das referidas células

Country Status (23)

Country Link
US (3) US10683495B2 (pt)
EP (1) EP3541947B1 (pt)
JP (2) JP7133226B2 (pt)
KR (1) KR102194520B1 (pt)
CN (1) CN109983130B (pt)
AR (1) AR110087A1 (pt)
AU (1) AU2017360666C1 (pt)
BR (1) BR112019008385A2 (pt)
CA (1) CA3043953A1 (pt)
DE (1) DE102016121899A1 (pt)
DK (1) DK3541947T3 (pt)
EA (1) EA201990758A1 (pt)
ES (1) ES2952039T3 (pt)
IL (1) IL266538A (pt)
MA (1) MA46835A (pt)
MX (1) MX2019005622A (pt)
MY (1) MY191776A (pt)
NZ (1) NZ752651A (pt)
PH (1) PH12019501001A1 (pt)
SG (1) SG11201903329WA (pt)
TW (1) TWI720268B (pt)
UA (1) UA125076C2 (pt)
WO (1) WO2018091396A1 (pt)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN118324918A (zh) 2017-11-06 2024-07-12 伊玛提克斯生物技术有限公司 新型改造t细胞受体及其免疫治疗的应用
DE102017125888A1 (de) 2017-11-06 2019-05-23 Immatics Biotechnologies Gmbh Neue konstruierte T-Zell-Rezeptoren und deren Verwendung in Immuntherapie
DE102019108125B4 (de) 2019-03-28 2022-02-03 Immatics US, Inc. Cd28 t-zellkulturen, zusammensetzungen und verfahren zu deren verwendung
WO2020191172A1 (en) 2019-03-19 2020-09-24 Immatics US, Inc. Cd28 t cell cultures, compositions, and methods of using thereof
DE102019121007A1 (de) 2019-08-02 2021-02-04 Immatics Biotechnologies Gmbh Antigenbindende Proteine, die spezifisch an MAGE-A binden
EP4090365A1 (en) 2020-01-15 2022-11-23 Immatics Biotechnologies GmbH Antigen binding proteins specifically binding prame
TW202309071A (zh) 2021-05-05 2023-03-01 德商英麥提克生物技術股份有限公司 特異性結合prame之抗原結合蛋白

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1471142T3 (da) 1991-04-10 2009-03-09 Scripps Research Inst Heterodimere receptor-biblioteker under anvendelse af fagemider
DK2193199T3 (da) 2007-08-20 2014-01-13 Nextera As Pvii fag-display
US9200270B2 (en) 2008-03-03 2015-12-01 Abbvie Inc. Methods for transforming yeast
JP5180326B2 (ja) 2008-03-13 2013-04-10 カーディアック ペースメイカーズ, インコーポレイテッド 自律神経系の緊張度を調整する医療システム
EA201390011A1 (ru) 2010-07-28 2013-07-30 Иммьюнокор Лтд. Т-клеточные рецепторы
WO2016102508A1 (en) 2014-12-22 2016-06-30 Universität Zürich Directed evolution of membrane proteins in eukaryotic cells with a cell wall

Also Published As

Publication number Publication date
JP2022130501A (ja) 2022-09-06
NZ752651A (en) 2022-01-28
ES2952039T3 (es) 2023-10-26
WO2018091396A1 (en) 2018-05-24
KR102194520B1 (ko) 2020-12-23
US20210355478A1 (en) 2021-11-18
TW201819621A (zh) 2018-06-01
EP3541947B1 (en) 2023-06-14
US20200263162A1 (en) 2020-08-20
PH12019501001A1 (en) 2019-11-11
TWI720268B (zh) 2021-03-01
AU2017360666B2 (en) 2021-01-07
MY191776A (en) 2022-07-14
CN109983130B (zh) 2023-10-20
SG11201903329WA (en) 2019-05-30
AU2017360666C1 (en) 2021-09-02
CA3043953A1 (en) 2018-05-24
US11104894B2 (en) 2021-08-31
DE102016121899A1 (de) 2018-05-17
MX2019005622A (es) 2019-08-14
JP7133226B2 (ja) 2022-09-08
DK3541947T3 (da) 2023-08-07
US20180135039A1 (en) 2018-05-17
AR110087A1 (es) 2019-02-20
EP3541947A1 (en) 2019-09-25
CN109983130A (zh) 2019-07-05
KR20190065400A (ko) 2019-06-11
UA125076C2 (uk) 2022-01-05
EA201990758A1 (ru) 2019-10-31
US10683495B2 (en) 2020-06-16
AU2017360666A1 (en) 2019-05-02
IL266538A (en) 2019-07-31
JP2019531751A (ja) 2019-11-07
MA46835A (fr) 2019-09-25

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B06W Patent application suspended after preliminary examination (for patents with searches from other patent authorities) chapter 6.23 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]