BR112019008385A2 - método de preparação de células de levedura eletrocompetentes e métodos de uso das referidas células - Google Patents
método de preparação de células de levedura eletrocompetentes e métodos de uso das referidas célulasInfo
- Publication number
- BR112019008385A2 BR112019008385A2 BR112019008385A BR112019008385A BR112019008385A2 BR 112019008385 A2 BR112019008385 A2 BR 112019008385A2 BR 112019008385 A BR112019008385 A BR 112019008385A BR 112019008385 A BR112019008385 A BR 112019008385A BR 112019008385 A2 BR112019008385 A2 BR 112019008385A2
- Authority
- BR
- Brazil
- Prior art keywords
- cells
- methods
- electrocompetent yeast
- yeast cells
- preparing electrocompetent
- Prior art date
Links
- 210000005253 yeast cell Anatomy 0.000 title abstract 3
- 210000004027 cell Anatomy 0.000 title 1
- 230000009466 transformation Effects 0.000 abstract 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract 1
- 238000004520 electroporation Methods 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/70503—Immunoglobulin superfamily
- C07K14/7051—T-cell receptor (TcR)-CD3 complex
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Cell Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Crystallography & Structural Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Botany (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
a presente invenção refere-se à transformação e melhoramento de células de levedura e a bibliotecas de células de levedura assim transformadas. mais especificamente, a presente invenção refere-se à transformação de leveduras por eletroporação.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE102016121899.5A DE102016121899A1 (de) | 2016-11-15 | 2016-11-15 | Verfahren zur Herstellung von elektrokompetenten Hefezellen und Verfahren zur Verwendung dieser Zellen |
PCT/EP2017/079011 WO2018091396A1 (en) | 2016-11-15 | 2017-11-13 | Method for preparing electrocompetent yeast cells, and method for using said cells |
Publications (1)
Publication Number | Publication Date |
---|---|
BR112019008385A2 true BR112019008385A2 (pt) | 2019-07-09 |
Family
ID=60484335
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112019008385A BR112019008385A2 (pt) | 2016-11-15 | 2017-11-13 | método de preparação de células de levedura eletrocompetentes e métodos de uso das referidas células |
Country Status (23)
Country | Link |
---|---|
US (3) | US10683495B2 (pt) |
EP (1) | EP3541947B1 (pt) |
JP (2) | JP7133226B2 (pt) |
KR (1) | KR102194520B1 (pt) |
CN (1) | CN109983130B (pt) |
AR (1) | AR110087A1 (pt) |
AU (1) | AU2017360666C1 (pt) |
BR (1) | BR112019008385A2 (pt) |
CA (1) | CA3043953A1 (pt) |
DE (1) | DE102016121899A1 (pt) |
DK (1) | DK3541947T3 (pt) |
EA (1) | EA201990758A1 (pt) |
ES (1) | ES2952039T3 (pt) |
IL (1) | IL266538A (pt) |
MA (1) | MA46835A (pt) |
MX (1) | MX2019005622A (pt) |
MY (1) | MY191776A (pt) |
NZ (1) | NZ752651A (pt) |
PH (1) | PH12019501001A1 (pt) |
SG (1) | SG11201903329WA (pt) |
TW (1) | TWI720268B (pt) |
UA (1) | UA125076C2 (pt) |
WO (1) | WO2018091396A1 (pt) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN118324918A (zh) | 2017-11-06 | 2024-07-12 | 伊玛提克斯生物技术有限公司 | 新型改造t细胞受体及其免疫治疗的应用 |
DE102017125888A1 (de) | 2017-11-06 | 2019-05-23 | Immatics Biotechnologies Gmbh | Neue konstruierte T-Zell-Rezeptoren und deren Verwendung in Immuntherapie |
DE102019108125B4 (de) | 2019-03-28 | 2022-02-03 | Immatics US, Inc. | Cd28 t-zellkulturen, zusammensetzungen und verfahren zu deren verwendung |
WO2020191172A1 (en) | 2019-03-19 | 2020-09-24 | Immatics US, Inc. | Cd28 t cell cultures, compositions, and methods of using thereof |
DE102019121007A1 (de) | 2019-08-02 | 2021-02-04 | Immatics Biotechnologies Gmbh | Antigenbindende Proteine, die spezifisch an MAGE-A binden |
EP4090365A1 (en) | 2020-01-15 | 2022-11-23 | Immatics Biotechnologies GmbH | Antigen binding proteins specifically binding prame |
TW202309071A (zh) | 2021-05-05 | 2023-03-01 | 德商英麥提克生物技術股份有限公司 | 特異性結合prame之抗原結合蛋白 |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DK1471142T3 (da) | 1991-04-10 | 2009-03-09 | Scripps Research Inst | Heterodimere receptor-biblioteker under anvendelse af fagemider |
DK2193199T3 (da) | 2007-08-20 | 2014-01-13 | Nextera As | Pvii fag-display |
US9200270B2 (en) | 2008-03-03 | 2015-12-01 | Abbvie Inc. | Methods for transforming yeast |
JP5180326B2 (ja) | 2008-03-13 | 2013-04-10 | カーディアック ペースメイカーズ, インコーポレイテッド | 自律神経系の緊張度を調整する医療システム |
EA201390011A1 (ru) | 2010-07-28 | 2013-07-30 | Иммьюнокор Лтд. | Т-клеточные рецепторы |
WO2016102508A1 (en) | 2014-12-22 | 2016-06-30 | Universität Zürich | Directed evolution of membrane proteins in eukaryotic cells with a cell wall |
-
2016
- 2016-11-15 DE DE102016121899.5A patent/DE102016121899A1/de not_active Withdrawn
-
2017
- 2017-11-13 EP EP17805126.4A patent/EP3541947B1/en active Active
- 2017-11-13 EA EA201990758A patent/EA201990758A1/ru unknown
- 2017-11-13 UA UAA201904554A patent/UA125076C2/uk unknown
- 2017-11-13 MA MA046835A patent/MA46835A/fr unknown
- 2017-11-13 JP JP2019521754A patent/JP7133226B2/ja active Active
- 2017-11-13 CA CA3043953A patent/CA3043953A1/en active Pending
- 2017-11-13 AU AU2017360666A patent/AU2017360666C1/en active Active
- 2017-11-13 MY MYPI2019002307A patent/MY191776A/en unknown
- 2017-11-13 KR KR1020197013641A patent/KR102194520B1/ko active IP Right Grant
- 2017-11-13 ES ES17805126T patent/ES2952039T3/es active Active
- 2017-11-13 WO PCT/EP2017/079011 patent/WO2018091396A1/en unknown
- 2017-11-13 MX MX2019005622A patent/MX2019005622A/es unknown
- 2017-11-13 CN CN201780069926.8A patent/CN109983130B/zh active Active
- 2017-11-13 SG SG11201903329WA patent/SG11201903329WA/en unknown
- 2017-11-13 DK DK17805126.4T patent/DK3541947T3/da active
- 2017-11-13 BR BR112019008385A patent/BR112019008385A2/pt not_active Application Discontinuation
- 2017-11-13 NZ NZ752651A patent/NZ752651A/en not_active IP Right Cessation
- 2017-11-15 US US15/813,879 patent/US10683495B2/en active Active
- 2017-11-15 TW TW106139472A patent/TWI720268B/zh active
- 2017-11-15 AR ARP170103182A patent/AR110087A1/es unknown
-
2019
- 2019-05-07 PH PH12019501001A patent/PH12019501001A1/en unknown
- 2019-05-08 IL IL266538A patent/IL266538A/en unknown
-
2020
- 2020-05-08 US US16/869,784 patent/US11104894B2/en active Active
-
2021
- 2021-07-29 US US17/389,061 patent/US20210355478A1/en active Pending
-
2022
- 2022-06-17 JP JP2022097799A patent/JP2022130501A/ja active Pending
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Legal Events
Date | Code | Title | Description |
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B350 | Update of information on the portal [chapter 15.35 patent gazette] | ||
B06W | Patent application suspended after preliminary examination (for patents with searches from other patent authorities) chapter 6.23 patent gazette] | ||
B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
B09B | Patent application refused [chapter 9.2 patent gazette] |