BR112017017975A2 - kit para preparação de amostra para detecção de anticorpo monoclonal - Google Patents
kit para preparação de amostra para detecção de anticorpo monoclonalInfo
- Publication number
- BR112017017975A2 BR112017017975A2 BR112017017975A BR112017017975A BR112017017975A2 BR 112017017975 A2 BR112017017975 A2 BR 112017017975A2 BR 112017017975 A BR112017017975 A BR 112017017975A BR 112017017975 A BR112017017975 A BR 112017017975A BR 112017017975 A2 BR112017017975 A2 BR 112017017975A2
- Authority
- BR
- Brazil
- Prior art keywords
- monoclonal antibody
- nanoparticles
- porous body
- sample preparation
- preparation kit
- Prior art date
Links
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/72—Mass spectrometers
- G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/62—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode
- G01N27/622—Ion mobility spectrometry
- G01N27/623—Ion mobility spectrometry combined with mass spectrometry
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6848—Methods of protein analysis involving mass spectrometry
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N2030/065—Preparation using different phases to separate parts of sample
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N2030/067—Preparation by reaction, e.g. derivatising the sample
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
- G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
- G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/901—Antibodies with enzymatic activity; e.g. abzymes
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Hydrology & Water Resources (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Electrochemistry (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Peptides Or Proteins (AREA)
Abstract
um kit de preparação de amostra relacionado à presente invenção fornece uma técnica analítica significantemente versátil que não é afetada pela diversidade de anticorpos, diferença em espécies, matriz e similares. para a preparação de uma amostra a ser usada para detecção de um anticorpo monoclonal através de cromatografia líquida de desempenho elevado-espectrometria de massa (lc-ms), o kit inclui um corpo poroso para a imobilização de um anticorpo monoclonal a ser detectado; nanopartículas com uma protease imobilizada; um vaso de reação para digerir seletivamente o anticorpo monoclonal conduzindo o corpo poroso e nanopartículas em contato; um tampão a ser introduzido no vaso de reação junto com as nanopartículas e corpo poroso a fim de que uma reação de protease seja realizada; e uma membrana de filtragem para remover o corpo poroso e nanopartículas após a proteólise a fim de extrair o produto de reação e o tampão.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2015047729 | 2015-03-10 | ||
PCT/JP2015/085459 WO2016143227A1 (ja) | 2015-03-10 | 2015-12-18 | モノクローナル抗体の検出のためのサンプル調製用キット |
Publications (1)
Publication Number | Publication Date |
---|---|
BR112017017975A2 true BR112017017975A2 (pt) | 2018-04-10 |
Family
ID=56879312
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112017017975A BR112017017975A2 (pt) | 2015-03-10 | 2015-12-18 | kit para preparação de amostra para detecção de anticorpo monoclonal |
Country Status (7)
Country | Link |
---|---|
US (2) | US20180052172A1 (pt) |
EP (1) | EP3270152A4 (pt) |
JP (1) | JP6515995B2 (pt) |
KR (1) | KR20170120178A (pt) |
CN (2) | CN115932065A (pt) |
BR (1) | BR112017017975A2 (pt) |
WO (1) | WO2016143227A1 (pt) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3270154B1 (en) * | 2015-03-09 | 2020-03-04 | Shimadzu Corporation | Method for detecting a monoclonal antibody using mass spectrometry |
JP6835260B2 (ja) * | 2017-12-28 | 2021-02-24 | 株式会社島津製作所 | モノクローナル抗体の簡素化された定量方法 |
CN111656190B (zh) * | 2018-02-08 | 2024-02-06 | 株式会社岛津制作所 | 改善单克隆抗体的检测结果的方法 |
JP7215467B2 (ja) * | 2019-08-19 | 2023-01-31 | 株式会社島津製作所 | ホルマリン固定パラフィン包埋組織試料からの抗体の分離、検出及び/又は分析方法 |
CN112326816B (zh) * | 2020-10-19 | 2021-07-20 | 广东莱恩医药研究院有限公司 | 一种定量检测血清中地舒单抗的液质联用方法 |
EP4365586A1 (en) | 2021-06-23 | 2024-05-08 | Shimadzu Corporation | Method for analyzing antibody |
CN114878289B (zh) * | 2022-07-12 | 2022-09-27 | 北京大学 | 一种耐低温低盐的蓝藻藻胆体样品的制备方法 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2003087821A2 (en) * | 2002-04-05 | 2003-10-23 | University Of Georgia Research Foundation | Method for cleaving and deglycosylating antibodies to promote ligand binding |
BRPI0607254A2 (pt) * | 2005-01-28 | 2009-08-25 | Susan Szathmary | composições imunologicamente ativa, método in vitro para identificação de peptìdeo ou proteìna, uso da composição e célula dendrìtica |
ATE529752T1 (de) * | 2006-12-21 | 2011-11-15 | Novartis Ag | Antikörperquantifizierung |
PT3023777T (pt) * | 2013-09-09 | 2018-01-19 | Shimadzu Corp | Método para preparar fragmentos peptídicos, kit para preparar fragmentos peptídicos para serem utilizados neste, e método de análise |
-
2015
- 2015-12-18 WO PCT/JP2015/085459 patent/WO2016143227A1/ja active Application Filing
- 2015-12-18 CN CN202210803075.8A patent/CN115932065A/zh active Pending
- 2015-12-18 EP EP15884711.1A patent/EP3270152A4/en not_active Withdrawn
- 2015-12-18 CN CN201580077602.XA patent/CN107407665A/zh active Pending
- 2015-12-18 BR BR112017017975A patent/BR112017017975A2/pt not_active Application Discontinuation
- 2015-12-18 JP JP2017504580A patent/JP6515995B2/ja active Active
- 2015-12-18 KR KR1020177027613A patent/KR20170120178A/ko not_active Application Discontinuation
- 2015-12-18 US US15/556,813 patent/US20180052172A1/en not_active Abandoned
-
2021
- 2021-11-16 US US17/455,089 patent/US20220137062A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
CN115932065A (zh) | 2023-04-07 |
KR20170120178A (ko) | 2017-10-30 |
CN107407665A (zh) | 2017-11-28 |
WO2016143227A1 (ja) | 2016-09-15 |
EP3270152A1 (en) | 2018-01-17 |
JPWO2016143227A1 (ja) | 2017-12-28 |
EP3270152A4 (en) | 2019-05-15 |
JP6515995B2 (ja) | 2019-05-22 |
US20220137062A1 (en) | 2022-05-05 |
US20180052172A1 (en) | 2018-02-22 |
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Legal Events
Date | Code | Title | Description |
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B06U | Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette] | ||
B11B | Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements |