BR112017017975A2 - kit para preparação de amostra para detecção de anticorpo monoclonal - Google Patents

kit para preparação de amostra para detecção de anticorpo monoclonal

Info

Publication number
BR112017017975A2
BR112017017975A2 BR112017017975A BR112017017975A BR112017017975A2 BR 112017017975 A2 BR112017017975 A2 BR 112017017975A2 BR 112017017975 A BR112017017975 A BR 112017017975A BR 112017017975 A BR112017017975 A BR 112017017975A BR 112017017975 A2 BR112017017975 A2 BR 112017017975A2
Authority
BR
Brazil
Prior art keywords
monoclonal antibody
nanoparticles
porous body
sample preparation
preparation kit
Prior art date
Application number
BR112017017975A
Other languages
English (en)
Inventor
Iwamoto Noriko
Shimada Takashi
Original Assignee
Shimadzu Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shimadzu Corp filed Critical Shimadzu Corp
Publication of BR112017017975A2 publication Critical patent/BR112017017975A2/pt

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/10—Devices for withdrawing samples in the liquid or fluent state
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/62—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode
    • G01N27/622—Ion mobility spectrometry
    • G01N27/623—Ion mobility spectrometry combined with mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G01N2030/065—Preparation using different phases to separate parts of sample
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G01N2030/067—Preparation by reaction, e.g. derivatising the sample
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/901—Antibodies with enzymatic activity; e.g. abzymes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Hydrology & Water Resources (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Electrochemistry (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Peptides Or Proteins (AREA)

Abstract

um kit de preparação de amostra relacionado à presente invenção fornece uma técnica analítica significantemente versátil que não é afetada pela diversidade de anticorpos, diferença em espécies, matriz e similares. para a preparação de uma amostra a ser usada para detecção de um anticorpo monoclonal através de cromatografia líquida de desempenho elevado-espectrometria de massa (lc-ms), o kit inclui um corpo poroso para a imobilização de um anticorpo monoclonal a ser detectado; nanopartículas com uma protease imobilizada; um vaso de reação para digerir seletivamente o anticorpo monoclonal conduzindo o corpo poroso e nanopartículas em contato; um tampão a ser introduzido no vaso de reação junto com as nanopartículas e corpo poroso a fim de que uma reação de protease seja realizada; e uma membrana de filtragem para remover o corpo poroso e nanopartículas após a proteólise a fim de extrair o produto de reação e o tampão.
BR112017017975A 2015-03-10 2015-12-18 kit para preparação de amostra para detecção de anticorpo monoclonal BR112017017975A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2015047729 2015-03-10
PCT/JP2015/085459 WO2016143227A1 (ja) 2015-03-10 2015-12-18 モノクローナル抗体の検出のためのサンプル調製用キット

Publications (1)

Publication Number Publication Date
BR112017017975A2 true BR112017017975A2 (pt) 2018-04-10

Family

ID=56879312

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017017975A BR112017017975A2 (pt) 2015-03-10 2015-12-18 kit para preparação de amostra para detecção de anticorpo monoclonal

Country Status (7)

Country Link
US (2) US20180052172A1 (pt)
EP (1) EP3270152A4 (pt)
JP (1) JP6515995B2 (pt)
KR (1) KR20170120178A (pt)
CN (2) CN115932065A (pt)
BR (1) BR112017017975A2 (pt)
WO (1) WO2016143227A1 (pt)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3270154B1 (en) * 2015-03-09 2020-03-04 Shimadzu Corporation Method for detecting a monoclonal antibody using mass spectrometry
WO2019130536A1 (ja) 2017-12-28 2019-07-04 株式会社島津製作所 モノクローナル抗体の簡素化された定量方法
JP7056675B2 (ja) * 2018-02-08 2022-04-19 株式会社島津製作所 モノクローナル抗体の検出結果を向上する方法
JP7215467B2 (ja) * 2019-08-19 2023-01-31 株式会社島津製作所 ホルマリン固定パラフィン包埋組織試料からの抗体の分離、検出及び／又は分析方法
CN112326816B (zh) * 2020-10-19 2021-07-20 广东莱恩医药研究院有限公司 一种定量检测血清中地舒单抗的液质联用方法
WO2022270126A1 (ja) 2021-06-23 2022-12-29 株式会社島津製作所 抗体を分析する方法
CN114878289B (zh) * 2022-07-12 2022-09-27 北京大学 一种耐低温低盐的蓝藻藻胆体样品的制备方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003221671A1 (en) * 2002-04-05 2003-10-27 University Of Georgia Research Foundation, Inc. Method for cleaving and deglycosylating antibodies to promote ligand binding
ZA200707249B (en) * 2005-01-28 2010-08-25 Galen Bio Inc Immunologically active compositions
US20100015652A1 (en) * 2006-12-21 2010-01-21 Brian Walter Granda Antibody quantitation
DK3023777T3 (en) * 2013-09-09 2018-01-15 Shimadzu Corp Method for Preparation of Peptide Fragments, Kits for Preparation of Peptide Fragments for Use Therein, and Method of Analysis

Also Published As

Publication number Publication date
US20180052172A1 (en) 2018-02-22
WO2016143227A1 (ja) 2016-09-15
US20220137062A1 (en) 2022-05-05
JPWO2016143227A1 (ja) 2017-12-28
JP6515995B2 (ja) 2019-05-22
CN115932065A (zh) 2023-04-07
CN107407665A (zh) 2017-11-28
KR20170120178A (ko) 2017-10-30
EP3270152A1 (en) 2018-01-17
EP3270152A4 (en) 2019-05-15

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B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements